NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14642R-1

Symbol

CG14642

Full Name

CG14642

CG No

CG14642

Old CG No

CG14642

Synonyms

SP29, CG14642

Accession No (Link to NCBI)

NM_141184.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Kambris Z, Brun S, Jang IH, Nam HJ, Romeo Y, Takahashi K, Lee WJ, Ueda R, Lemaitre B.<br> Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation.<br> Curr Biol (2006) 16(8) 808-13 [ PubMed ID = 16631589 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGCAATCTGTCCGTGTGTCTG

Primer Seq. 3'

aatctagaggtaccTGCCCTCTGTCCGACTCGAAG

Predicted Fragment Size

IR fragment full Seq

0001 CGCAATCTGT CCGTGTGTCT GATATCATTT ATCGGGCTCT GGTGCCTGAG CAATACTCAC 
0061 ACCCAGCGCC TGCCCCCAGA AgGGCGAATG CGCCCCTTGC AAGACGACTC GATCCGCTCT 
0121 CCCGTGGACC GCGACATAGT CTTTCCGGAG TTAGACGCGG GACCAGGCAA GCCCGAGGAG 
0181 AAGATGTGGT TTCACATAAC AGACTTTCAA TTTGATCGGG TGGAGGGCCC GACCCAGCCC 
0241 AAGCCCAAGC CCAGACAATA CCCCCCGCCG CCGATGCCCG GCCAGCCCTT TCCGCCGCCC 
0301 CCCGGGGGCT TTAAAAAGAA GGAAAACAAG CAACGCCGCC TCTGCGAGCA AAAATATTCC 
0361 GAATATGTGG AGCGCATCTT TCCCAATGAT ACCGCAGTGG CTGCCGATGC CAACGACGCA 
0421 GACTTCGATG GTCGGGTACC TGGCCCGGCC CGGCTGNCGT GGGCTATCGA GTCGGACAGA 
0481 GGGCA

in silico PCR Fragment

0001 CGCAATCTGT CCGTGTGTCT GATATCATTT ATCGGGCTCT GGTGCCTGAG CAATACTCAC 
0061 ACCCAGCGCC TGCCCCCAGA AGGGCGAATG CGCCCCTTGC AAGACGACTC GATCCGCTCT 
0121 CCCGTGGACC GCGACATAGT CTTTCCGGAG TTAGACGCGG GACCAGGCAA GCCCGAGGAG 
0181 AAGATGTGGT TTCACATAAC AGACTTTCAA TTTGATCGGG TGGAGGGCCC GACCCAGCCC 
0241 AAGCCCAAGC CCAGACAATA CCCCCCGCCG CCGATGCCCG GCCAGCCCTT TCCGCCGCCC 
0301 CCCGGGGGCT TTAAAAAGAA GGAAAACAAG CAACGCCGCC TCTGCGAGCA AAAATATTCC 
0361 GAATATGTGG AGCGCATCTT TCCCAATGAT ACCGCAGTGG CTGCCGATGC CAACGACGCA 
0421 GACTTCGATG GTCGGGT-CC TGGCCCGGCC CGGCTGCCGT GGGCT-TCGA GTCGGACAGA 
0481 GGGCA

Assemble Data

<PRE>

14642R-1.IR_full       1   CGCAATCTGTCCGTGTGTCTGATATCATTTATCGGGCTCTGGTGCCTGAGCAATACTCAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14642R-1.in silico     1   CGCAATCTGTCCGTGTGTCTGATATCATTTATCGGGCTCTGGTGCCTGAGCAATACTCAC 60


14642R-1.IR_full       61  ACCCAGCGCCTGCCCCCAGAAGGGCGAATGCGCCCCTTGCAAGACGACTCGATCCGCTCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14642R-1.in silico     61  ACCCAGCGCCTGCCCCCAGAAGGGCGAATGCGCCCCTTGCAAGACGACTCGATCCGCTCT 120


14642R-1.IR_full       121 CCCGTGGACCGCGACATAGTCTTTCCGGAGTTAGACGCGGGACCAGGCAAGCCCGAGGAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14642R-1.in silico     121 CCCGTGGACCGCGACATAGTCTTTCCGGAGTTAGACGCGGGACCAGGCAAGCCCGAGGAG 180


14642R-1.IR_full       181 AAGATGTGGTTTCACATAACAGACTTTCAATTTGATCGGGTGGAGGGCCCGACCCAGCCC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14642R-1.in silico     181 AAGATGTGGTTTCACATAACAGACTTTCAATTTGATCGGGTGGAGGGCCCGACCCAGCCC 240


14642R-1.IR_full       241 AAGCCCAAGCCCAGACAATACCCCCCGCCGCCGATGCCCGGCCAGCCCTTTCCGCCGCCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14642R-1.in silico     241 AAGCCCAAGCCCAGACAATACCCCCCGCCGCCGATGCCCGGCCAGCCCTTTCCGCCGCCC 300


14642R-1.IR_full       301 CCCGGGGGCTTTAAAAAGAAGGAAAACAAGCAACGCCGCCTCTGCGAGCAAAAATATTCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14642R-1.in silico     301 CCCGGGGGCTTTAAAAAGAAGGAAAACAAGCAACGCCGCCTCTGCGAGCAAAAATATTCC 360


14642R-1.IR_full       361 GAATATGTGGAGCGCATCTTTCCCAATGATACCGCAGTGGCTGCCGATGCCAACGACGCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14642R-1.in silico     361 GAATATGTGGAGCGCATCTTTCCCAATGATACCGCAGTGGCTGCCGATGCCAACGACGCA 420


14642R-1.IR_full       421 GACTTCGATGGTCGGGT<span class="snp"><tt>A</tt></span>CCTGGCCCGGCCCGGCTG<span class="snp"><tt>N</tt></span>CGTGGGCT<span class="snp"><tt>A</tt></span>TCGAGTCGGACAGA 480
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||
14642R-1.in silico     421 GACTTCGATGGTCGGGT<span class="snp"><tt>-</tt></span>CCTGGCCCGGCCCGGCTG<span class="snp"><tt>C</tt></span>CGTGGGCT<span class="snp"><tt>-</tt></span>TCGAGTCGGACAGA 480


14642R-1.IR_full       481 GGGCA 485
                           |||||
14642R-1.in silico     481 GGGCA 485

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_164322.1	CG14642-RB, transcript variant B (CG14642), mRNA
93.15	449	2	2	6	NM_141184.2	CG14642-RA, transcript variant A (CG14642), mRNA
0.2	1	5	7	12	NM_140149.2	CG8003-RA (CG8003), mRNA
0	0	5	17	19	NM_080312.2	CG7803-RA, transcript variant A (z), mRNA
0	0	5	17	19	NM_001038738.1	CG7803-RB, transcript variant B (z), mRNA
0	0	2	4	5	NM_133081.1	CG15047-RA (CG15047), mRNA
0	0	2	3	2	NM_132650.1	CG10617-RA (CG10617), mRNA
0	0	2	3	2	NM_168124.1	CG10596-RC, transcript variant C (Msr-110), mRNA
0	0	2	3	2	NM_168123.1	CG10596-RA, transcript variant A (Msr-110), mRNA
0	0	2	3	2	NM_139716.2	CG10596-RB, transcript variant B (Msr-110), mRNA
0	0	2	2	7	NM_001042830.1	CG41474-RA (CG41474), mRNA
0	0	1	4	8	NM_134962.2	CG31772-RA (CG31772), mRNA
0	0	1	4	4	NM_057419.2	CG5619-RA (trk), mRNA
0	0	1	4	2	NM_132858.2	CG9056-RA (CG9056), mRNA
0	0	1	4	2	NM_136508.2	CG8711-RA (cul-4), mRNA
0	0	1	4	2	NM_137043.2	CG13333-RA (CG13333), mRNA
0	0	1	3	4	NM_057469.3	CG10718-RA (neb), mRNA
0	0	1	3	4	NM_169733.2	CG10325-RB, transcript variant B (abd-A), mRNA
0	0	1	3	4	NM_057345.2	CG10325-RA, transcript variant A (abd-A), mRNA
0	0	1	3	3	NM_135691.1	CG6734-RA (CG6734), mRNA
0	0	1	2	5	NM_168087.1	CG32241-RA (CG32241), mRNA
0	0	1	2	5	NM_137034.2	CG17064-RA (mars), mRNA
0	0	1	2	4	NM_136687.2	CG1472-RA (sec24), mRNA
0	0	1	2	2	NM_132621.1	CG12715-RA (CG12715), mRNA
0	0	1	2	2	NM_169036.1	CG31543-RC, transcript variant C (Hph), mRNA
0	0	1	2	2	NM_141268.2	CG31543-RA, transcript variant A (Hph), mRNA
0	0	1	2	2	NM_169037.1	CG31543-RB, transcript variant B (Hph), mRNA
0	0	0	6	9	NM_164524.1	CG9660-RD, transcript variant D (toc), mRNA
0	0	0	6	9	NM_057805.2	CG9660-RA, transcript variant A (toc), mRNA
0	0	0	6	3	NM_164953.1	CG16833-RB, transcript variant B (CG16833), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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