National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14641R-1 
 Symbol CG14641  Full Name CG14641 
 CG No CG14641  Old CG No CG14641 
 Synonyms cg14641, CG14641 
 Accession No (Link to NCBI) NM_141183.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.<br> A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.<br> Dev Cell (2010) 18(5) 862-76 [ PubMed ID = 20493818 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGGCAGAACTGGGAAGATGCT 
 Primer Seq. 3' 
aatctagaggtaccTCCTCTCCACGCTTGCACTCG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGGCAGAACT GGGAAGATGC TGAATTCCCA ATTCTGTGCC AAACTTGCCT TGNCGACAAT 
0061 CCGTACGTGC GGATGATTAA AGAGCGGTTC GGCAAGGAAT GCAAGATATG CACGCGTCCA 
0121 TTCACAATAT TTCGATGGTG TCCTGGTGCT CGTATGCGCT TCAAAAAGAC GGAGATATGT 
0181 CAAACGTGTG CGCGCCTGAA GAACGTGTGT CAGACCTGCC TCTTGGACCT GGAGTACGGA 
0241 CTGCCGATTC AAGTACGAGA TGCGGCGCTC AAAGTGGCTG ACAATATGCC ACAAAGCGAC 
0301 GTTAACAAGG AATACTACAT CCAAAACATA GACGCCCAGC TGCAGGACGG AGATGGCACG 
0361 GAGGCGGCAG GAGCTGTCGG TCGATCCCTC GCCGCCAACG AGATGTTGTC CAAgcTTGCG 
0421 CgCACAGCAC CCTATTACAA AAGAAATCGG CCGCACATCT GCTCCTTTTG GGTTAAGGGC 
0481 GAGTGCAAGC GTGGAGAGGA  
 in silico PCR Fragment 
0001 CGGCAGAACT GGGAAGATGC TGAATTCCCA ATTCTGTGCC AAACTTGCCT TGGCGACAAT 
0061 CCGTACGTGC GGATGATTAA AGAGCGGTTC GGCAAGGAAT GCAAGATATG CACGCGTCCA 
0121 TTCACAATAT TTCGATGGTG TCCTGGTGCT CGTATGCGCT TCAAAAAGAC GGAGATATGT 
0181 CAAACGTGTG CGCGCCTGAA GAACGTGTGT CAGACCTGCC TCTTGGACCT GGAGTACGGA 
0241 CTGCCGATTC AAGTACGAGA TGCGGCGCTC AAAGTGGCTG ACAATATGCC ACAAAGCGAC 
0301 GTTAACAAGG AATACTACAT CCAAAACATA GACGCCCAGC TGCAGGACGG AGATGGCACG 
0361 GAGGCGGCAG GAGCTGTCGG TCGATCCCTC GCCGCCAACG AGATGTTGTC CAAGCTTGCG 
0421 CGCACAGCAC CCTATTACAA AAGAAATCGG CCGCACATCT GCTCCTTTTG GGTTAAGGGC 
0481 GAGTGCAAGC GTGGAGAGGA  
 Assemble Data 
<PRE>

14641R-1.IR_full       1   CGGCAGAACTGGGAAGATGCTGAATTCCCAATTCTGTGCCAAACTTGCCTTG<span class="snp"><tt>N</tt></span>CGACAAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
14641R-1.in silico     1   CGGCAGAACTGGGAAGATGCTGAATTCCCAATTCTGTGCCAAACTTGCCTTG<span class="snp"><tt>G</tt></span>CGACAAT 60


14641R-1.IR_full       61  CCGTACGTGCGGATGATTAAAGAGCGGTTCGGCAAGGAATGCAAGATATGCACGCGTCCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14641R-1.in silico     61  CCGTACGTGCGGATGATTAAAGAGCGGTTCGGCAAGGAATGCAAGATATGCACGCGTCCA 120


14641R-1.IR_full       121 TTCACAATATTTCGATGGTGTCCTGGTGCTCGTATGCGCTTCAAAAAGACGGAGATATGT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14641R-1.in silico     121 TTCACAATATTTCGATGGTGTCCTGGTGCTCGTATGCGCTTCAAAAAGACGGAGATATGT 180


14641R-1.IR_full       181 CAAACGTGTGCGCGCCTGAAGAACGTGTGTCAGACCTGCCTCTTGGACCTGGAGTACGGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14641R-1.in silico     181 CAAACGTGTGCGCGCCTGAAGAACGTGTGTCAGACCTGCCTCTTGGACCTGGAGTACGGA 240


14641R-1.IR_full       241 CTGCCGATTCAAGTACGAGATGCGGCGCTCAAAGTGGCTGACAATATGCCACAAAGCGAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14641R-1.in silico     241 CTGCCGATTCAAGTACGAGATGCGGCGCTCAAAGTGGCTGACAATATGCCACAAAGCGAC 300


14641R-1.IR_full       301 GTTAACAAGGAATACTACATCCAAAACATAGACGCCCAGCTGCAGGACGGAGATGGCACG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14641R-1.in silico     301 GTTAACAAGGAATACTACATCCAAAACATAGACGCCCAGCTGCAGGACGGAGATGGCACG 360


14641R-1.IR_full       361 GAGGCGGCAGGAGCTGTCGGTCGATCCCTCGCCGCCAACGAGATGTTGTCCAAGCTTGCG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14641R-1.in silico     361 GAGGCGGCAGGAGCTGTCGGTCGATCCCTCGCCGCCAACGAGATGTTGTCCAAGCTTGCG 420


14641R-1.IR_full       421 CGCACAGCACCCTATTACAAAAGAAATCGGCCGCACATCTGCTCCTTTTGGGTTAAGGGC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14641R-1.in silico     421 CGCACAGCACCCTATTACAAAAGAAATCGGCCGCACATCTGCTCCTTTTGGGTTAAGGGC 480


14641R-1.IR_full       481 GAGTGCAAGCGTGGAGAGGA 500
                           ||||||||||||||||||||
14641R-1.in silico     481 GAGTGCAAGCGTGGAGAGGA 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_141183.2  CG14641-RA (CG14641), mRNA 
0.2   1  2  3  2  NM_167196.1  CG12141-RB, transcript variant B (Aats-lys), mRNA 
0.2   1  2  3  2  NM_132345.1  CG12141-RA, transcript variant A (Aats-lys), mRNA 
0   0  1  4  2  NM_132302.3  CG7055-RA (dalao), mRNA 
0   0  1  4  2  NM_167045.1  CG32758-RA (CG32758), mRNA 
0   0  1  4  2  NM_136895.2  CG13176-RA (CG13176), mRNA 
0   0  1  3  3  NM_166757.1  CG1710-RC, transcript variant C (Hcf), mRNA 
0   0  1  3  3  NM_166756.1  CG1710-RB, transcript variant B (Hcf), mRNA 
0   0  1  3  3  NM_079882.2  CG1710-RA, transcript variant A (Hcf), mRNA 
0   0  1  3  3  NM_205873.1  CG1710-RD, transcript variant D (Hcf), mRNA 
0   0  1  3  3  NM_140898.3  CG32217-RA (Su(Tpl)), mRNA 
0   0  1  2  3  NM_139448.2  CG32300-RB (oxt), mRNA 
0   0  1  2  2  NM_176699.1  CG2977-RA, transcript variant A (inx7), mRNA 
0   0  1  2  2  NM_176698.1  CG2977-RB, transcript variant B (inx7), mRNA 
0   0  0  4  5  NM_134605.2  CG1696-RA (l(1)G0269), mRNA 
0   0  0  4  4  NM_169996.1  CG5740-RA, transcript variant A (CG5740), mRNA 
0   0  0  4  4  NM_142753.2  CG5740-RB, transcript variant B (CG5740), mRNA 
0   0  0  4  3  NM_132738.1  CG9413-RA, transcript variant A (CG9413), mRNA 
0   0  0  4  3  NM_167411.1  CG9413-RB, transcript variant B (CG9413), mRNA 
0   0  0  4  2  NM_165152.1  CG5848-RA, transcript variant A (cact), mRNA 
0   0  0  4  2  NM_057594.3  CG5848-RB, transcript variant B (cact), mRNA 
0   0  0  4  2  NM_205999.1  CG5848-RD, transcript variant D (cact), mRNA 
0   0  0  4  2  NM_057595.3  CG5848-RC, transcript variant C (cact), mRNA 
0   0  0  3  6  NM_058067.2  CG6147-RA (Tsc1), mRNA 
0   0  0  3  5  NM_079913.2  CG8110-RA, transcript variant A (syd), mRNA 
0   0  0  3  5  NM_137739.2  CG10795-RA (CG10795), mRNA 
0   0  0  3  5  NM_130526.2  CG3655-RA (CG3655), mRNA 
0   0  0  3  4  NM_164931.1  CG31719-RB, transcript variant B (RluA-1), mRNA 
0   0  0  3  4  NM_164932.1  CG31719-RC, transcript variant C (RluA-1), mRNA 
0   0  0  3  4  NM_164930.1  CG31719-RA, transcript variant A (RluA-1), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme03040 : Spliceosome

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.