NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

1458R-2

Symbol

CG1458

Full Name

CG1458

CG No

CG1458

Old CG No

CG1458

Synonyms

BcDNA:RE49709, CG1458

Accession No (Link to NCBI)

NM_143427.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGAGCCCATATCACATCTG

Primer Seq. 3'

aatctagaggtaccTTGATGACAATTGGTCCGACG

Predicted Fragment Size

IR fragment full Seq

0001 ATGGAGCCCA TATCACATCT GGTGAAGTCC TCGCTGCCCA ATTACTTGTC AAGTCTGCCG 
0061 GTTCCCGACA GCATCGGCGG CTGGTTTAAG CTCTCCTTCA AGGATTGGTT GGCCCTGATC 
0121 CCACCCACCG TGGTGGTGGC CGGACNTCgG CTACACCGCC TACCTGGCCT ACTGTCCGGC 
0181 GGCACGGGCC AGCTGCGCGG CCAAAAACAG CGGACGCTGC AACAACCACA TCCGCAAGAA 
0241 CGAGCCCAAG GTGGTGGACA TGATCGACGT GGAGGATATT GCGGAGAAGG CGGCCTTCTG 
0301 TCGCTGCTGG AAGACCAAGA ACTGGCCCTA CTGCGATGGC AGTCATGGCG AGCACAACAA 
0361 GCAGACTGGA GACAACGTCG GACCAATTGT CATCAA

in silico PCR Fragment

0001 ATGGAGCCCA TATCACATCT GGTGAAGTCC TCGCTGCCCA ATTACTTGTC AAGTCTGCCG 
0061 GTTCCCGACA GCATCGGCGG CTGGTTTAAG CTCTCCTTCA AGGATTGGTT GGCCCTGATC 
0121 CCACCCACCG TGGTGGTGGC CGGAC-TCGG CTACACCGCC TACCTGGCCT ACTGTCCGGC 
0181 GGCACGGGCC AGCTGCGCGG CCAAAAACAG CGGACGCTGC AACAACCACA TCCGCAAGAA 
0241 CGAGCCCAAG GTGGTGGACA TGATCGACGT GGAGGATATT GCGGAGAAGG CGGCCTTCTG 
0301 TCGCTGCTGG AAGACCAAGA ACTGGCCCTA CTGCGATGGC AGTCATGGCG AGCACAACAA 
0361 GCAGACTGGA GACAACGTCG GACCAATTGT CATCAA

Assemble Data

<PRE>

1458R-2.IR_full       1   ATGGAGCCCATATCACATCTGGTGAAGTCCTCGCTGCCCAATTACTTGTCAAGTCTGCCG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1458R-2.in silico     1   ATGGAGCCCATATCACATCTGGTGAAGTCCTCGCTGCCCAATTACTTGTCAAGTCTGCCG 60


1458R-2.IR_full       61  GTTCCCGACAGCATCGGCGGCTGGTTTAAGCTCTCCTTCAAGGATTGGTTGGCCCTGATC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1458R-2.in silico     61  GTTCCCGACAGCATCGGCGGCTGGTTTAAGCTCTCCTTCAAGGATTGGTTGGCCCTGATC 120


1458R-2.IR_full       121 CCACCCACCGTGGTGGTGGCCGGAC<span class="snp"><tt>N</tt></span>TCGGCTACACCGCCTACCTGGCCTACTGTCCGGC 180
                          |||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||
1458R-2.in silico     121 CCACCCACCGTGGTGGTGGCCGGAC<span class="snp"><tt>-</tt></span>TCGGCTACACCGCCTACCTGGCCTACTGTCCGGC 180


1458R-2.IR_full       181 GGCACGGGCCAGCTGCGCGGCCAAAAACAGCGGACGCTGCAACAACCACATCCGCAAGAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1458R-2.in silico     181 GGCACGGGCCAGCTGCGCGGCCAAAAACAGCGGACGCTGCAACAACCACATCCGCAAGAA 240


1458R-2.IR_full       241 CGAGCCCAAGGTGGTGGACATGATCGACGTGGAGGATATTGCGGAGAAGGCGGCCTTCTG 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1458R-2.in silico     241 CGAGCCCAAGGTGGTGGACATGATCGACGTGGAGGATATTGCGGAGAAGGCGGCCTTCTG 300


1458R-2.IR_full       301 TCGCTGCTGGAAGACCAAGAACTGGCCCTACTGCGATGGCAGTCATGGCGAGCACAACAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1458R-2.in silico     301 TCGCTGCTGGAAGACCAAGAACTGGCCCTACTGCGATGGCAGTCATGGCGAGCACAACAA 360


1458R-2.IR_full       361 GCAGACTGGAGACAACGTCGGACCAATTGTCATCAA 396
                          ||||||||||||||||||||||||||||||||||||
1458R-2.in silico     361 GCAGACTGGAGACAACGTCGGACCAATTGTCATCAA 396

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	377	0	0	0	NM_143427.2	CG1458-RA (CG1458), mRNA
0	0	3	3	6	NM_133012.2	CG32560-RA (CG32560), mRNA
0	0	3	3	6	NM_165630.1	CG8266-RB, transcript variant B (sec31), mRNA
0	0	3	3	6	NM_136570.2	CG8266-RA, transcript variant A (sec31), mRNA
0	0	3	2	3	NM_079861.2	CG1455-RA (CanA1), mRNA
0	0	2	4	2	NM_143451.2	CG18287-RA (ppk19), mRNA
0	0	2	2	3	NM_141099.1	CG14573-RA (CG14573), mRNA
0	0	1	6	3	NM_132352.2	CG1354-RA, transcript variant A (CG1354), mRNA
0	0	1	6	3	NM_167202.2	CG1354-RD, transcript variant D (CG1354), mRNA
0	0	1	6	3	NM_167201.2	CG1354-RC, transcript variant C (CG1354), mRNA
0	0	1	4	3	NM_142762.2	CG7080-RA (CG7080), mRNA
0	0	1	3	4	NM_130641.2	CG2865-RA (CG2865), mRNA
0	0	1	3	3	NM_079090.2	CG3668-RA (fd59A), mRNA
0	0	1	2	6	NM_134789.1	CG15356-RA (CG15356), mRNA
0	0	1	2	3	NM_132363.1	CG1791-RA (CG1791), mRNA
0	0	1	2	3	NM_143533.1	CG2218-RA (CG2218), mRNA
0	0	1	2	3	NM_079112.3	CG3140-RA (Adk2), mRNA
0	0	1	2	2	NM_170187.1	CG31238-RA (CG31238), mRNA
0	0	0	8	3	NM_057902.3	CG7486-RC, transcript variant C (Dredd), mRNA
0	0	0	8	3	NM_057901.3	CG7486-RB, transcript variant B (Dredd), mRNA
0	0	0	8	3	NM_057903.3	CG7486-RA, transcript variant A (Dredd), mRNA
0	0	0	7	29	NM_168626.1	CG7439-RC, transcript variant C (AGO2), mRNA
0	0	0	7	29	NM_140518.1	CG7439-RB, transcript variant B (AGO2), mRNA
0	0	0	6	4	NM_141312.2	CG1245-RA (MED27), mRNA
0	0	0	6	3	NM_136073.2	CG18397-RA (CG18397), mRNA
0	0	0	5	10	NM_080366.2	CG2238-RA, transcript variant A (Ef2b), mRNA
0	0	0	5	10	NM_165395.1	CG2238-RC, transcript variant C (Ef2b), mRNA
0	0	0	5	10	NM_165394.1	CG2238-RB, transcript variant B (Ef2b), mRNA
0	0	0	5	5	NM_079618.2	CG8599-RA (Su(var)3-7), mRNA
0	0	0	4	9	NM_001038742.1	CG6824-RD, transcript variant D (ovo), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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