NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14561R-1

Symbol

CG14561

Full Name

CG14561

CG No

CG14561

Old CG No

CG14561

Synonyms

CG14561

Accession No (Link to NCBI)

NM_141120.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCAGTGCTCGATGTCCTCCGT

Primer Seq. 3'

aatctagaggtaccGCGAGGGTAAATGCGGGTCAG

Predicted Fragment Size

IR fragment full Seq

0001 GCAGTGCTCG ATGTCCTCCG TGGATNATGG AGAGCGAGAG TGACCTCTCG YCTGGCATTC 
0061 GAGCGCGAGN AGCAAATACC GGATGGCGGC TNCCGACGCN DCTGCCGGCC TTCGAGATCC 
0121 GCGCCTTCNT CGCCGCATGG ACGCACCCCG TCGCCCATCG ACGATCTCGA TCTGTCGGAC 
0181 ATCGAGACGC CCAAGCAGCC GCAGANGCAG CATCTCCTTC CGGAAGAGGA ACCCCAGAAC 
0241 GATCCTCAGT ACGTTGCCCT ACACGAGATA AACGCCCAGA TCAGCCCGCC CAATGACGCC 
0301 GACGACTCCA ACAGCTTGGA GACGATCTTC GAGGGCGTTT TCCTGAGCAC CCCgCCCCGC 
0361 GATAAAgNTT CCAGCTCGGG CAATTCCCGA TTTACgCCCA AGCGCGGACG TGCAAATCTC 
0421 ATCGAGCTGA TgGNCAACCG GCTGCACGGC GGCAAGGANA ACCAGTCGCC GGGAGCgNGA 
0481 GTCTCTGACC CGCATTTACC NTNGC

in silico PCR Fragment

0001 GCAGTGCTCG ATGTCCTCCG TGGAT-ATGG AGAGCGAGAG TGACCTCTCG -CTGGCATTC 
0061 GAGCGCGAGG AGCAAATACC GGATGGCGGC T-GCGACG-A GCTGCCGGCC TTCGAGATCC 
0121 GCGCCTTC-T CGCCGCATGG ACGCACCCCG TCGCCCATCG ACGATCTCGA TCTGTCGGAC 
0181 ATCGAGACGC CCAAGCAGCC GCAGAGGCAG CATCTCCTTC CGGAAGAGGA ACCCCAGAAC 
0241 GATCCTCAGT ACGTTGCCCT ACACGAGATA AACGCCCAGA TCAGCCCGCC CAATGACGCC 
0301 GACGACTCCA ACAGCTTGGA GACGATCTTC GAGGGCGTTT TCCTGAGCAC CCCGCCCCGC 
0361 GATAAAGCTT CCAGCTCGGG CAATTCCCGA TTTACGCCCA AGCGCGGACG TGCAAATCTC 
0421 ATCGAGCTGA TGGCCAACCG GCTGCACGGC GGCAAGGAGA ACCAGTCGCC GGGAGCGCGA 
0481 GTCTCTGACC CGCATTTACC CTCGC

Assemble Data

<PRE>

14561R-1.IR_full       1   GCAGTGCTCGATGTCCTCCGTGGAT<span class="snp"><tt>N</tt></span>ATGGAGAGCGAGAGTGACCTCTCG<span class="snp"><tt>Y</tt></span>CTGGCATTC 60
                           |||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||
14561R-1.in silico     1   GCAGTGCTCGATGTCCTCCGTGGAT<span class="snp"><tt>-</tt></span>ATGGAGAGCGAGAGTGACCTCTCG<span class="snp"><tt>-</tt></span>CTGGCATTC 60


14561R-1.IR_full       61  GAGCGCGAG<span class="snp"><tt>N</tt></span>AGCAAATACCGGATGGCGGCT<span class="snp"><tt>N</tt></span><span class="snp"><tt>C</tt></span>CGACG<span class="snp"><tt>C</tt></span><span class="snp"><tt>N</tt></span><span class="snp"><tt>D</tt></span>CTGCCGGCCTTCGAGATCC 120
                           |||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
14561R-1.in silico     61  GAGCGCGAG<span class="snp"><tt>G</tt></span>AGCAAATACCGGATGGCGGCT<span class="snp"><tt>-</tt></span><span class="snp"><tt>G</tt></span>CGACG<span class="snp"><tt>-</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span>CTGCCGGCCTTCGAGATCC 120


14561R-1.IR_full       121 GCGCCTTC<span class="snp"><tt>N</tt></span>TCGCCGCATGGACGCACCCCGTCGCCCATCGACGATCTCGATCTGTCGGAC 180
                           ||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||
14561R-1.in silico     121 GCGCCTTC<span class="snp"><tt>-</tt></span>TCGCCGCATGGACGCACCCCGTCGCCCATCGACGATCTCGATCTGTCGGAC 180


14561R-1.IR_full       181 ATCGAGACGCCCAAGCAGCCGCAGA<span class="snp"><tt>N</tt></span>GCAGCATCTCCTTCCGGAAGAGGAACCCCAGAAC 240
                           |||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||
14561R-1.in silico     181 ATCGAGACGCCCAAGCAGCCGCAGA<span class="snp"><tt>G</tt></span>GCAGCATCTCCTTCCGGAAGAGGAACCCCAGAAC 240


14561R-1.IR_full       241 GATCCTCAGTACGTTGCCCTACACGAGATAAACGCCCAGATCAGCCCGCCCAATGACGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14561R-1.in silico     241 GATCCTCAGTACGTTGCCCTACACGAGATAAACGCCCAGATCAGCCCGCCCAATGACGCC 300


14561R-1.IR_full       301 GACGACTCCAACAGCTTGGAGACGATCTTCGAGGGCGTTTTCCTGAGCACCCCGCCCCGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14561R-1.in silico     301 GACGACTCCAACAGCTTGGAGACGATCTTCGAGGGCGTTTTCCTGAGCACCCCGCCCCGC 360


14561R-1.IR_full       361 GATAAAG<span class="snp"><tt>N</tt></span>TTCCAGCTCGGGCAATTCCCGATTTACGCCCAAGCGCGGACGTGCAAATCTC 420
                           |||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||
14561R-1.in silico     361 GATAAAG<span class="snp"><tt>C</tt></span>TTCCAGCTCGGGCAATTCCCGATTTACGCCCAAGCGCGGACGTGCAAATCTC 420


14561R-1.IR_full       421 ATCGAGCTGATGG<span class="snp"><tt>N</tt></span>CAACCGGCTGCACGGCGGCAAGGA<span class="snp"><tt>N</tt></span>AACCAGTCGCCGGGAGCG<span class="snp"><tt>N</tt></span>GA 480
                           |||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||
14561R-1.in silico     421 ATCGAGCTGATGG<span class="snp"><tt>C</tt></span>CAACCGGCTGCACGGCGGCAAGGA<span class="snp"><tt>G</tt></span>AACCAGTCGCCGGGAGCG<span class="snp"><tt>C</tt></span>GA 480


14561R-1.IR_full       481 GTCTCTGACCCGCATTTACC<span class="snp"><tt>N</tt></span>T<span class="snp"><tt>N</tt></span>GC 505
                           ||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|<span class="snp"><tt>&nbsp;</tt></span>||
14561R-1.in silico     481 GTCTCTGACCCGCATTTACC<span class="snp"><tt>C</tt></span>T<span class="snp"><tt>C</tt></span>GC 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141120.1	CG14561-RA (CG14561), mRNA
0	0	5	5	2	NM_137294.1	CG8317-RA (CG8317), mRNA
0	0	5	2	2	NM_144050.1	CG12848-RA (CG12848), mRNA
0	0	3	6	3	NM_130617.2	CG4281-RA (CG4281), mRNA
0	0	2	3	4	NM_134770.1	CG17657-RA (CG17657), mRNA
0	0	2	3	3	NM_142663.3	CG15695-RA (CG15695), mRNA
0	0	2	3	2	NM_176686.1	CG33080-RB, transcript variant B (CG33080), mRNA
0	0	2	3	2	NM_176685.1	CG33080-RA, transcript variant A (CG33080), mRNA
0	0	2	2	11	NM_132420.2	CG32676-RA (CG32676), mRNA
0	0	2	2	3	NM_137120.1	CG17386-RA (CG17386), mRNA
0	0	1	4	6	NM_142237.2	CG4606-RA (alpha-Man-IIb), mRNA
0	0	1	4	6	NM_170184.1	CG6238-RB, transcript variant B (ssh), mRNA
0	0	1	4	6	NM_079768.2	CG6238-RA, transcript variant A (ssh), mRNA
0	0	1	4	3	NM_079632.4	CG4264-RA, transcript variant A (Hsc70-4), mRNA
0	0	1	4	3	NM_176502.1	CG4264-RE, transcript variant E (Hsc70-4), mRNA
0	0	1	4	3	NM_169626.1	CG4264-RC, transcript variant C (Hsc70-4), mRNA
0	0	1	4	3	NM_169627.1	CG4264-RD, transcript variant D (Hsc70-4), mRNA
0	0	1	4	3	NM_176503.1	CG4264-RF, transcript variant F (Hsc70-4), mRNA
0	0	1	4	3	NM_169625.1	CG4264-RB, transcript variant B (Hsc70-4), mRNA
0	0	1	3	6	NM_141317.2	CG2108-RA (Rab23), mRNA
0	0	1	3	3	NM_168569.1	CG8937-RD, transcript variant D (Hsc70-1), mRNA
0	0	1	3	3	NM_079339.2	CG8937-RA, transcript variant A (Hsc70-1), mRNA
0	0	1	3	3	NM_168568.2	CG8937-RC, transcript variant C (Hsc70-1), mRNA
0	0	1	3	2	NM_139804.1	CG14821-RA (CG14821), mRNA
0	0	1	3	2	NM_137380.2	CG4866-RA (CG4866), mRNA
0	0	1	2	10	NM_132665.1	CG15753-RA (CG15753), mRNA
0	0	1	2	7	NM_170030.1	CG5248-RD, transcript variant D (loco), mRNA
0	0	1	2	6	NM_139833.1	CG8616-RA (CG8616), mRNA
0	0	1	2	2	NM_079203.2	CG11348-RA, transcript variant A (nAcRbeta-64B), mRNA
0	0	0	8	16	NM_166019.1	CG18076-RH, transcript variant H (shot), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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