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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14514R-1

Symbol

Brd8

Full Name

Brd8

CG No

CG14514

Old CG No

CG14514

Synonyms

dBrd8, CG14514, Brd8

Accession No (Link to NCBI)

NM_143428.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCGACCAGAACTGGATCACG

Primer Seq. 3'

aatctagaggtaccTGGTTGGAACGCTTGACGGCT

Predicted Fragment Size

IR fragment full Seq

0001 gGNGANCCAG AACTGGATCA CGGTGAGCCG CACGCTGAAG ACGATCTGCG GCAATGGCAG 
0061 CAGTAACCGA CCGGCGGACT GGTTCTCGCA GAAGAACTGT GCCATGCAGT ACGGCAATCT 
0121 GCTGGAGAGC GTGGAGGCCA CCAAGCGCAA GAAGCgCAGC AGCGANAAGN NGTGCCGGCG 
0181 TCTCCTCGCC GGCCACCGTG GAGACGCCCA CAGAATTGCT GCTCCGTCGG CTCACGGAGG 
0241 AGCGTCAGGC GGAGATCANA GGCGCAGATG CGTCAGGATC AGGAGACTTA CCGACGCATC 
0301 CAGCGGGAGA TTGAGTCGCT GCAATCGGAC GCGGTGACCG AGCAGGAGCT GCAGGACATG 
0361 TGGCTGGAGA TCGAGAAGGA GCAGGANGGC GCGCCGCATC GAGGAGATGA AGCTGGAGAA 
0421 TCGCATGCGG GAGNGCGAGC AGCGCAAGAA GGACCTGGCC AGCAACTGGC GAAACAGCAG 
0481 TCTAGCCGTC AAGCGTTCCN AACCA

in silico PCR Fragment

0001 GGCGA-CCAG AACTGGATCA CGGTGAGCCG CACGCTGAAG ACGATCTGCG GCAATGGCAG 
0061 CAGTAACCGA CCGGCGGACT GGTTCTCGCA GAAGAACTGT GCCATGCAGT ACGGCAATCT 
0121 GCTGGAGAGC GTGGAGGCCA CCAAGCGCAA GAAGCGCAGC AGCGA-AAGC AGTGCCGGCG 
0181 TCTCCTCGCC GGCCACCGTG GAGACGCCCA CAGAATTGCT GCTCCGTCGG CTCACGGAGG 
0241 AGCGTCAGGC GGAGATCA-A GGCGCAGATG CGTCAGGATC AGGAGACTTA CCGACGCATC 
0301 CAGCGGGAGA TTGAGTCGCT GCAATCGGAC GCGGTGACCG AGCAGGAGCT GCAGGACATG 
0361 TGGCTGGAGA TCGAGAAGGA GCAGGA-GGC GCGCCGCATC GAGGAGATGA AGCTGGAGAA 
0421 TCGCATGCGG GAGCGCGAGC AGCGCAAGAA GGACCTGGCC AGCAACTGGC GAAACAGCAG 
0481 TCTAGCCGTC AAGCGTTCC- AACCA

Assemble Data

<PRE>

14514R-1.IR_full       1   GG<span class="snp"><tt>N</tt></span>GA<span class="snp"><tt>N</tt></span>CCAGAACTGGATCACGGTGAGCCGCACGCTGAAGACGATCTGCGGCAATGGCAG 60
                           ||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||
14514R-1.in silico     1   GG<span class="snp"><tt>C</tt></span>GA<span class="snp"><tt>-</tt></span>CCAGAACTGGATCACGGTGAGCCGCACGCTGAAGACGATCTGCGGCAATGGCAG 60


14514R-1.IR_full       61  CAGTAACCGACCGGCGGACTGGTTCTCGCAGAAGAACTGTGCCATGCAGTACGGCAATCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14514R-1.in silico     61  CAGTAACCGACCGGCGGACTGGTTCTCGCAGAAGAACTGTGCCATGCAGTACGGCAATCT 120


14514R-1.IR_full       121 GCTGGAGAGCGTGGAGGCCACCAAGCGCAAGAAGCGCAGCAGCGA<span class="snp"><tt>N</tt></span>AAG<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>GTGCCGGCG 180
                           |||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||
14514R-1.in silico     121 GCTGGAGAGCGTGGAGGCCACCAAGCGCAAGAAGCGCAGCAGCGA<span class="snp"><tt>-</tt></span>AAG<span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span>GTGCCGGCG 180


14514R-1.IR_full       181 TCTCCTCGCCGGCCACCGTGGAGACGCCCACAGAATTGCTGCTCCGTCGGCTCACGGAGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14514R-1.in silico     181 TCTCCTCGCCGGCCACCGTGGAGACGCCCACAGAATTGCTGCTCCGTCGGCTCACGGAGG 240


14514R-1.IR_full       241 AGCGTCAGGCGGAGATCA<span class="snp"><tt>N</tt></span>AGGCGCAGATGCGTCAGGATCAGGAGACTTACCGACGCATC 300
                           ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||
14514R-1.in silico     241 AGCGTCAGGCGGAGATCA<span class="snp"><tt>-</tt></span>AGGCGCAGATGCGTCAGGATCAGGAGACTTACCGACGCATC 300


14514R-1.IR_full       301 CAGCGGGAGATTGAGTCGCTGCAATCGGACGCGGTGACCGAGCAGGAGCTGCAGGACATG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14514R-1.in silico     301 CAGCGGGAGATTGAGTCGCTGCAATCGGACGCGGTGACCGAGCAGGAGCTGCAGGACATG 360


14514R-1.IR_full       361 TGGCTGGAGATCGAGAAGGAGCAGGA<span class="snp"><tt>N</tt></span>GGCGCGCCGCATCGAGGAGATGAAGCTGGAGAA 420
                           ||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||
14514R-1.in silico     361 TGGCTGGAGATCGAGAAGGAGCAGGA<span class="snp"><tt>-</tt></span>GGCGCGCCGCATCGAGGAGATGAAGCTGGAGAA 420


14514R-1.IR_full       421 TCGCATGCGGGAG<span class="snp"><tt>N</tt></span>GCGAGCAGCGCAAGAAGGACCTGGCCAGCAACTGGCGAAACAGCAG 480
                           |||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||
14514R-1.in silico     421 TCGCATGCGGGAG<span class="snp"><tt>C</tt></span>GCGAGCAGCGCAAGAAGGACCTGGCCAGCAACTGGCGAAACAGCAG 480


14514R-1.IR_full       481 TCTAGCCGTCAAGCGTTCC<span class="snp"><tt>N</tt></span>AACCA 505
                           |||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||
14514R-1.in silico     481 TCTAGCCGTCAAGCGTTCC<span class="snp"><tt>-</tt></span>AACCA 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_143428.2	CG14514-RA (Brd8), mRNA
0.41	2	3	2	5	NM_143619.2	CG11522-RB, transcript variant B (RpL6), mRNA
0.41	2	3	2	5	NM_170554.1	CG11522-RA, transcript variant A (RpL6), mRNA
0.2	1	2	3	2	NM_176384.2	CG10508-RC, transcript variant C (CG10508), mRNA
0.2	1	2	3	2	NM_206412.1	CG10508-RF, transcript variant F (CG10508), mRNA
0	0	4	4	3	NM_135798.2	CG9306-RA (CG9306), mRNA
0	0	4	3	5	NM_136854.1	CG13194-RA (pyr), mRNA
0	0	4	3	4	NM_001014604.1	CG9795-RD, transcript variant D (CG9795), mRNA
0	0	4	3	4	NM_141197.3	CG9795-RA, transcript variant A (CG9795), mRNA
0	0	4	3	4	NM_176396.1	CG9795-RC, transcript variant C (CG9795), mRNA
0	0	4	3	4	NM_164338.2	CG9795-RB, transcript variant B (CG9795), mRNA
0	0	3	4	3	NM_170485.1	CG2139-RC, transcript variant C (aralar1), mRNA
0	0	3	4	3	NM_170487.1	CG2139-RB, transcript variant B (aralar1), mRNA
0	0	3	4	3	NM_143538.2	CG2139-RA, transcript variant A (aralar1), mRNA
0	0	3	4	3	NM_170486.1	CG2139-RD, transcript variant D (aralar1), mRNA
0	0	3	3	11	NM_166318.1	CG15105-RB, transcript variant B (CG15105), mRNA
0	0	3	3	11	NM_137546.2	CG15105-RA, transcript variant A (CG15105), mRNA
0	0	3	3	3	NM_079956.2	CG18455-RA, transcript variant A (Optix), mRNA
0	0	3	2	7	NM_206295.1	CG33275-RB, transcript variant B (CG33275), mRNA
0	0	3	2	7	NM_206294.1	CG33275-RA, transcript variant A (CG33275), mRNA
0	0	3	2	4	NM_166402.1	CG30147-RB, transcript variant B (Hil), mRNA
0	0	3	2	4	NM_137649.2	CG30147-RA, transcript variant A (Hil), mRNA
0	0	3	2	2	NM_168319.1	CG4760-RA, transcript variant A (bol), mRNA
0	0	3	2	2	NM_079265.2	CG4760-RB, transcript variant B (bol), mRNA
0	0	3	2	2	NM_168318.1	CG4760-RD, transcript variant D (bol), mRNA
0	0	3	2	2	NM_168320.1	CG4760-RC, transcript variant C (bol), mRNA
0	0	2	4	9	NM_205886.1	CG11838-RB, transcript variant B (Oseg3), mRNA
0	0	2	3	13	NM_168103.1	CG7507-RB, transcript variant B (Dhc64C), mRNA
0	0	2	3	7	NM_166927.1	CG3981-RA, transcript variant A (Unc-76), mRNA
0	0	2	3	7	NM_130625.2	CG3981-RB, transcript variant B (Unc-76), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme03082 : ATP-dependent chromatin remodeling

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