NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14510Ra-4

Symbol

CG14509

Full Name

CG14509

CG No

CG14509

Old CG No

CG14510

Synonyms

CG14510, CG14509

Accession No (Link to NCBI)

NM_176582.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCTCCATCTGCGATCCCAAG

Primer Seq. 3'

aatctagaggtaccACTCGGAATGGGAGCCTAGCG

Predicted Fragment Size

IR fragment full Seq

0001 GGCTCCATCT GCGATCCCAA GANGANGTCC TGCCAGTGCA CCGAGGATCT GCCCGTCACC 
0061 AATCACTATG ACAAATGTGG CAAAGAGGCC GCCGTGAACG AGTCCTGCTT CTTTAACGAG 
0121 CAGTGCGAGA TGAGATACTT CCAGACGGAG TGCCGCGATG GACGCTGCAT CTGCCGGTTC 
0181 GAGATGTCAC CCATTTGGGG CAAGGACGGA TCCGTGGAGT GCAAAGGGCG CCAGGACAAG 
0241 AGGGGACCCG AGACCTACAT CGACCCGGCC ATGATTGGCG TGCTGGTAGG AATGGCATTG 
0301 ATGTTTGTTA TTATTTGTGT CGTCCTGCGA TTGTTTAGCC AAGCTCGCTG GCGTGAGAAC 
0361 CGAACCATCT TCAACACGCC CAATCCTCGC CTGATGAACG TCTCCCTGCT GCGGGACAGC 
0421 AAGCTGCTCC ACGGCCAGGA GCGCCGAGGG TCCAGGATGT CGGTGCGGGC GCCATCTCGT 
0481 CAGCCCAGCA TGGCCTNCCT GCGGCCCCAC TCCCCCAATC CGNNGCTAGG CTCCCATTCC 
0541 GAGT

in silico PCR Fragment

0001 GGCTCCATCT GCGATCCCAA GAAGAAGTCC TGCCAGTGCA CCGAGGATCT GCCCGTCACC 
0061 AATCACTATG ACAAATGTGG CAAAGATGAG ATACTTCCAG ACGGAGTGCC GCGATGGACG 
0121 CTGCATCTGC CGGTTCGAGA TGTCACCCAT TTGGGGCAAG GACGGATCCG TGGAGTGCAA 
0181 AGGGCGCCAG GACAAGAGGG GACCCGAGAC CTACATCGAC CCGGCCATGA TTGGCGTGCT 
0241 GGTAGGAATG GCATTGATGT TTGTTATTAT TTGTGTCGTC CTGCGATTGT TTAGCCAAGC 
0301 TCGCTGGCGT GAGAACCGAA CCATCTTCAA CACGCCCAAT CCTCGCCTGA TGAACGTCTC 
0361 CCTGCTGCGG GACAGCAAGC TGCTCCACGG CCAGGAGCGC CGAGGGTCCA GGATGTCGGT 
0421 GCGAGCGCCA TCTCGTCAGC CCAGCATGGC CTCCCTGCGG CCCCACTCCC CCAATCCGTC 
0481 GCTAGGCTCC CATTCCGAGT

Assemble Data

<PRE>

14510Ra-4.IR full       1   GGCTCCATCTGCGATCCCAAGA<span class="snp"><tt>N</tt></span>GA<span class="snp"><tt>N</tt></span>GTCCTGCCAGTGCACCGAGGATCTGCCCGTCACC 60
                            ||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||
14510Ra-4.in silico     1   GGCTCCATCTGCGATCCCAAGA<span class="snp"><tt>A</tt></span>GA<span class="snp"><tt>A</tt></span>GTCCTGCCAGTGCACCGAGGATCTGCCCGTCACC 60


14510Ra-4.IR full       61  AATCACTATGACAAATGTGGCAAAGA<span class="snp"><tt>G</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span> 120
                            ||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>
14510Ra-4.in silico     61  AATCACTATGACAAATGTGGCAAAGA<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span> 120


14510Ra-4.IR full       121 <span class="snp"><tt>C</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span><span class="snp"><tt>G</tt></span><span class="snp"><tt>A</tt></span>TGAGATACTTCCAGACGGAGTGCCGCGATGGACGCTGCATCTGCCGGTTC 180
                            <span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||
14510Ra-4.in silico     121 <span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>TGAGATACTTCCAGACGGAGTGCCGCGATGGACGCTGCATCTGCCGGTTC 180


14510Ra-4.IR full       181 GAGATGTCACCCATTTGGGGCAAGGACGGATCCGTGGAGTGCAAAGGGCGCCAGGACAAG 240
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14510Ra-4.in silico     181 GAGATGTCACCCATTTGGGGCAAGGACGGATCCGTGGAGTGCAAAGGGCGCCAGGACAAG 240


14510Ra-4.IR full       241 AGGGGACCCGAGACCTACATCGACCCGGCCATGATTGGCGTGCTGGTAGGAATGGCATTG 300
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14510Ra-4.in silico     241 AGGGGACCCGAGACCTACATCGACCCGGCCATGATTGGCGTGCTGGTAGGAATGGCATTG 300


14510Ra-4.IR full       301 ATGTTTGTTATTATTTGTGTCGTCCTGCGATTGTTTAGCCAAGCTCGCTGGCGTGAGAAC 360
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14510Ra-4.in silico     301 ATGTTTGTTATTATTTGTGTCGTCCTGCGATTGTTTAGCCAAGCTCGCTGGCGTGAGAAC 360


14510Ra-4.IR full       361 CGAACCATCTTCAACACGCCCAATCCTCGCCTGATGAACGTCTCCCTGCTGCGGGACAGC 420
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14510Ra-4.in silico     361 CGAACCATCTTCAACACGCCCAATCCTCGCCTGATGAACGTCTCCCTGCTGCGGGACAGC 420


14510Ra-4.IR full       421 AAGCTGCTCCACGGCCAGGAGCGCCGAGGGTCCAGGATGTCGGTGCG<span class="snp"><tt>G</tt></span>GCGCCATCTCGT 480
                            |||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||
14510Ra-4.in silico     421 AAGCTGCTCCACGGCCAGGAGCGCCGAGGGTCCAGGATGTCGGTGCG<span class="snp"><tt>A</tt></span>GCGCCATCTCGT 480


14510Ra-4.IR full       481 CAGCCCAGCATGGCCT<span class="snp"><tt>N</tt></span>CCTGCGGCCCCACTCCCCCAATCCG<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>GCTAGGCTCCCATTCC 540
                            ||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||
14510Ra-4.in silico     481 CAGCCCAGCATGGCCT<span class="snp"><tt>C</tt></span>CCTGCGGCCCCACTCCCCCAATCCG<span class="snp"><tt>T</tt></span><span class="snp"><tt>C</tt></span>GCTAGGCTCCCATTCC 540


14510Ra-4.IR full       541 GAGT 544
                            ||||
14510Ra-4.in silico     541 GAGT 544

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_176582.1	CG14509-RA (CG14509), mRNA
0	0	3	2	6	NM_137993.2	CG4735-RA (shu), mRNA
0	0	2	2	3	NM_144058.2	CG30158-RA (CG30158), mRNA
0	0	1	3	6	NM_142861.2	CG6697-RA (CG6697), mRNA
0	0	1	3	4	NM_134281.1	CG8651-RC, transcript variant C (trx), mRNA
0	0	1	3	4	NM_001014621.1	CG8651-RE, transcript variant E (trx), mRNA
0	0	1	3	4	NM_134282.1	CG8651-RA, transcript variant A (trx), mRNA
0	0	1	3	4	NM_057421.2	CG8651-RD, transcript variant D (trx), mRNA
0	0	1	3	4	NM_057422.2	CG8651-RB, transcript variant B (trx), mRNA
0	0	1	3	2	NM_169717.1	CG31279-RA (CG31279), mRNA
0	0	0	5	7	NM_079175.2	CG1242-RA (Hsp83), mRNA
0	0	0	4	6	NM_141666.2	CG9492-RA (CG9492), mRNA
0	0	0	4	5	NM_137628.1	CG13868-RA (CG13868), mRNA
0	0	0	4	5	NM_136982.2	CG3850-RA (sug), mRNA
0	0	0	4	5	NM_134833.4	CG9866-RA, transcript variant A (CG9866), mRNA
0	0	0	4	5	NM_164481.1	CG9866-RB, transcript variant B (CG9866), mRNA
0	0	0	4	2	NM_135125.1	CG14000-RA (CG14000), mRNA
0	0	0	4	2	NM_143555.2	CG9717-RA (CG9717), mRNA
0	0	0	3	12	NM_206586.1	CG31038-RC, transcript variant C (CG31038), mRNA
0	0	0	3	11	NM_206585.1	CG31038-RE, transcript variant E (CG31038), mRNA
0	0	0	3	11	NM_206584.1	CG31038-RF, transcript variant F (CG31038), mRNA
0	0	0	3	11	NM_170447.1	CG31038-RA, transcript variant A (CG31038), mRNA
0	0	0	3	10	NM_057326.4	CG12212-RA, transcript variant A (peb), mRNA
0	0	0	3	10	NM_001014722.1	CG12212-RB, transcript variant B (peb), mRNA
0	0	0	3	7	NM_078977.2	CG10897-RA, transcript variant A (tou), mRNA
0	0	0	3	7	NM_170040.2	CG31139-RA (CG31139), mRNA
0	0	0	3	7	NM_206138.1	CG15920-RB, transcript variant B (resilin), mRNA
0	0	0	3	7	NM_137313.2	CG15920-RA, transcript variant A (resilin), mRNA
0	0	0	3	6	NM_001031866.1	CG33950-RA, transcript variant A (trol), mRNA
0	0	0	3	6	NM_001031864.1	CG33950-RE, transcript variant E (trol), mRNA
100.83	485	75	146	174	NM_176582.1	CG14509-RA (CG14509), mRNA
40.95	197	885	2345	3073	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
40.95	197	885	2345	3073	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
40.95	197	885	2345	3073	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
11.22	54	334	995	1674	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
11.22	54	334	995	1674	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
10.18	49	225	607	1074	NM_079671.2	CG7847-RA, transcript variant A (sr), mRNA
10.18	49	206	503	836	NM_169786.1	CG7847-RB, transcript variant B (sr), mRNA
9.97	48	200	424	552	NM_001014565.1	CG10491-RB, transcript variant B (vn), mRNA
9.97	48	200	424	552	NM_079218.2	CG10491-RA, transcript variant A (vn), mRNA
9.77	47	220	596	1042	NM_131975.3	CG32767-RA, transcript variant A (CG32767), mRNA
9.77	47	220	596	1042	NM_206628.1	CG32767-RB, transcript variant B (CG32767), mRNA
9.77	47	132	193	151	NM_144114.3	CG14494-RA (CG14494), mRNA
9.77	47	128	192	107	NM_144133.1	CG13235-RA (CG13235), mRNA
8.52	41	239	794	1476	NM_001038734.1	CG16902-RC (Hr4), mRNA
8.52	41	145	313	538	NM_167239.2	CG32677-RA (CG32677), mRNA
8.31	40	148	332	495	NM_001038965.1	CG9924-RE, transcript variant E (CG9924), mRNA
8.31	40	118	267	293	NM_057678.3	CG31240-RA (repo), mRNA
7.9	38	109	161	125	NM_136061.1	CG15166-RA (CG15166), mRNA
7.69	37	137	344	511	NM_132172.2	CG15478-RA (CG15478), mRNA
7.48	36	180	491	741	NM_134474.4	CG32532-RA (CG32532), mRNA
7.48	36	129	219	327	NM_133012.2	CG32560-RA (CG32560), mRNA
7.06	34	92	143	112	NM_132301.3	CG7065-RA (CG7065), mRNA
6.86	33	92	126	92	NM_176711.2	CG1543-RB (Tbh), mRNA
6.65	32	135	363	619	NM_132569.1	CG11245-RA (CG11245), mRNA
6.44	31	132	363	441	NM_169657.1	CG5166-RB, transcript variant B (Atx2), mRNA
6.44	31	132	360	431	NM_169658.2	CG5166-RC, transcript variant C (Atx2), mRNA
6.44	31	132	360	431	NM_142209.2	CG5166-RA, transcript variant A (Atx2), mRNA
6.44	31	127	275	314	NM_135957.2	CG31738-RB, transcript variant B (CG31738), mRNA
6.44	31	80	120	97	NM_057368.3	CG2621-RD, transcript variant D (sgg), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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