NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14508R-2

Symbol

CG14508

Full Name

CG14508

CG No

CG14508

Old CG No

CG14508

Synonyms

CG14508

Accession No (Link to NCBI)

NM_143426.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

pupal lethal

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.<br> Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.<br> Genetics (2012) 192(2) 741-52 [ PubMed ID = 22798488 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCGGTGCGTCCGCGGATAAGT

Primer Seq. 3'

aatctagaggtaccCCCTTGCGAGCCGACACAATA

Predicted Fragment Size

IR fragment full Seq

0001 TCGGTGCGTC CGCGGATAAG TTTTCCGGGT CGTGGAAGCG GCTTTACTGG AAATTCGCAA 
0061 GCTGCTGGGA GCCCTGGGAG CCCTAACCGG AACAGCAGGG CTGHTGATCT ACGCCCTGGA 
0121 AACCAGCGTG GATGCCTCCT CGGACTGCGT GCATCCGGCC CACCAGCACT GGAATCACAA 
0181 AGGGCTTTTG TCCGCCTTGG ACAAGGAGAG CGTGCGTCGA GGTTACACCG TTTACAAGGA 
0241 GGTCTGCTCC TCCTGCCACT CATTGCAGTA CATGGCCTAT CGCAACCTGG TGGGAGTGTG 
0301 CATGACGGAG GCGGAGGCCA AGGCCGAGGC GGTAGGCCAT CACGGTGCGG GATGGGCCCA 
0361 ACGAGGAGGG CGAGTACTAC GAGCGTCCCG GCAAGCTGTC CGATCACTTC CCCTCTCCGT 
0421 ACGCCAACGA GGAGGCGGCC CGTTCCGCCA ACAATGGCTC CTATCCGCCG GATCTCAGCT 
0481 ATATTGTGTC GGCTCGCAAG GG

in silico PCR Fragment

0001 TCGGTGCGTC CGCGGATAAG TTTTCCGGGT CGTGGAAGCG GCTTTACTGG AAA-TCGCAA 
0061 GCTGCTGGGA GCCCTGGGAG CCCTAACCGG AACAGCAGGG CTGCTGATCT ACGCCCTGGA 
0121 AACCAGCGTG GATGCCTCCT CGGACTGCGT GCATCCGGCC CACCAGCACT GGAATCACAA 
0181 AGGGCTTTTG TCCGCCTTGG ACAAGGAGAG CGTGCGTCGA GGTTACACCG TTTACAAGGA 
0241 GGTCTGCTCC TCCTGCCACT CATTGCAGTA CATGGCCTAT CGCAACCTGG TGGGAGTGTG 
0301 CATGACGGAG GCGGAGGCCA AGGCCGAGGC GG-AGGCCAT CACGGTGCGG GATGGGCCCA 
0361 ACGAGGAGGG CGAGTACTAC GAGCGTCCCG GCAAGCTGTC CGATCACTTC CCCTCTCCGT 
0421 ACGCCAACGA GGAGGCGGCC CGTTCCGCCA ACAATGGCTC CTATCCGCCG GATCTCAGCT 
0481 ATATTGTGTC GGCTCGCAAG GG

Assemble Data

<PRE>

14508R-2.IR_full       1   TCGGTGCGTCCGCGGATAAGTTTTCCGGGTCGTGGAAGCGGCTTTACTGGAAA<span class="snp"><tt>T</tt></span>TCGCAA 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||
14508R-2.in silico     1   TCGGTGCGTCCGCGGATAAGTTTTCCGGGTCGTGGAAGCGGCTTTACTGGAAA<span class="snp"><tt>-</tt></span>TCGCAA 60


14508R-2.IR_full       61  GCTGCTGGGAGCCCTGGGAGCCCTAACCGGAACAGCAGGGCTG<span class="snp"><tt>H</tt></span>TGATCTACGCCCTGGA 120
                           |||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||
14508R-2.in silico     61  GCTGCTGGGAGCCCTGGGAGCCCTAACCGGAACAGCAGGGCTG<span class="snp"><tt>C</tt></span>TGATCTACGCCCTGGA 120


14508R-2.IR_full       121 AACCAGCGTGGATGCCTCCTCGGACTGCGTGCATCCGGCCCACCAGCACTGGAATCACAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14508R-2.in silico     121 AACCAGCGTGGATGCCTCCTCGGACTGCGTGCATCCGGCCCACCAGCACTGGAATCACAA 180


14508R-2.IR_full       181 AGGGCTTTTGTCCGCCTTGGACAAGGAGAGCGTGCGTCGAGGTTACACCGTTTACAAGGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14508R-2.in silico     181 AGGGCTTTTGTCCGCCTTGGACAAGGAGAGCGTGCGTCGAGGTTACACCGTTTACAAGGA 240


14508R-2.IR_full       241 GGTCTGCTCCTCCTGCCACTCATTGCAGTACATGGCCTATCGCAACCTGGTGGGAGTGTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14508R-2.in silico     241 GGTCTGCTCCTCCTGCCACTCATTGCAGTACATGGCCTATCGCAACCTGGTGGGAGTGTG 300


14508R-2.IR_full       301 CATGACGGAGGCGGAGGCCAAGGCCGAGGCGG<span class="snp"><tt>T</tt></span>AGGCCATCACGGTGCGGGATGGGCCCA 360
                           ||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||
14508R-2.in silico     301 CATGACGGAGGCGGAGGCCAAGGCCGAGGCGG<span class="snp"><tt>-</tt></span>AGGCCATCACGGTGCGGGATGGGCCCA 360


14508R-2.IR_full       361 ACGAGGAGGGCGAGTACTACGAGCGTCCCGGCAAGCTGTCCGATCACTTCCCCTCTCCGT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14508R-2.in silico     361 ACGAGGAGGGCGAGTACTACGAGCGTCCCGGCAAGCTGTCCGATCACTTCCCCTCTCCGT 420


14508R-2.IR_full       421 ACGCCAACGAGGAGGCGGCCCGTTCCGCCAACAATGGCTCCTATCCGCCGGATCTCAGCT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14508R-2.in silico     421 ACGCCAACGAGGAGGCGGCCCGTTCCGCCAACAATGGCTCCTATCCGCCGGATCTCAGCT 480


14508R-2.IR_full       481 ATATTGTGTCGGCTCGCAAGGG 502
                           ||||||||||||||||||||||
14508R-2.in silico     481 ATATTGTGTCGGCTCGCAAGGG 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	10	NM_143426.2	CG14508-RA (CG14508), mRNA
0.41	2	3	25	71	NM_139701.1	CG4769-RA (CG4769), mRNA
0.2	1	2	2	6	NM_206691.2	CG1817-RA, transcript variant A (Ptp10D), mRNA
0.2	1	2	2	6	NM_167292.2	CG1817-RB, transcript variant B (Ptp10D), mRNA
0.2	1	2	2	6	NM_206690.1	CG1817-RD, transcript variant D (Ptp10D), mRNA
0	0	5	2	2	NM_167012.1	CG7035-RA, transcript variant A (Cbp80), mRNA
0	0	5	2	2	NM_080011.2	CG7035-RB, transcript variant B (Cbp80), mRNA
0	0	4	2	7	NM_078574.2	CG1830-RA, transcript variant A (PhKgamma), mRNA
0	0	4	2	7	NM_167297.1	CG1830-RC, transcript variant C (PhKgamma), mRNA
0	0	3	26	33	NM_132120.1	CG14438-RA, transcript variant A (CG14438), mRNA
0	0	3	26	33	NM_167090.1	CG14438-RB, transcript variant B (CG14438), mRNA
0	0	3	3	4	NM_134994.1	CG15435-RA (CG15435), mRNA
0	0	2	6	13	NM_078846.2	CG4182-RA (yellow-c), mRNA
0	0	2	3	11	NM_135652.2	CG6287-RA (CG6287), mRNA
0	0	2	3	10	NM_135694.2	CG6785-RA (CG6785), mRNA
0	0	2	2	6	NM_141526.2	CG9617-RA (CG9617), mRNA
0	0	1	7	6	NM_078740.2	CG8817-RB, transcript variant B (lilli), mRNA
0	0	1	7	6	NM_164516.1	CG8817-RA, transcript variant A (lilli), mRNA
0	0	1	7	6	NM_164517.1	CG8817-RC, transcript variant C (lilli), mRNA
0	0	1	6	9	NM_136710.2	CG1381-RA (CG1381), mRNA
0	0	1	6	2	NM_001038857.1	CG8585-RD, transcript variant D (Ih), mRNA
0	0	1	6	2	NM_001038858.1	CG8585-RB, transcript variant B (Ih), mRNA
0	0	1	6	2	NM_001038859.1	CG8585-RE, transcript variant E (Ih), mRNA
0	0	1	6	2	NM_001038856.1	CG8585-RC, transcript variant C (Ih), mRNA
0	0	1	5	13	NM_001043070.1	CG34146-RA (brp), mRNA
0	0	1	5	12	NM_168064.2	CG14998-RA, transcript variant A (CG14998), mRNA
0	0	1	5	12	NM_168063.2	CG14998-RD, transcript variant D (CG14998), mRNA
0	0	1	5	12	NM_168062.2	CG14998-RE, transcript variant E (CG14998), mRNA
0	0	1	5	12	NM_139604.2	CG14998-RC, transcript variant C (CG14998), mRNA
0	0	1	5	12	NM_168061.2	CG14998-RB, transcript variant B (CG14998), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

dme00190 : Oxidative phosphorylation
dme01100 : Metabolic pathways

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