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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
14174R-2 |
| Symbol |
CG14174 |
Full Name |
CG14174 |
| CG No |
CG14174 |
Old CG No |
CG14174 |
| Synonyms |
CG14174 |
| Accession No (Link to NCBI) |
NM_140094.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAACAACCTCCGTTGGTGAC |
| Primer Seq. 3' |
aatctagaggtaccGCAAGGAGCTCAGTTTGCTAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NAAACAACCT CCGTTGGTGA CGCTGCTGGT GAAGGACACG GGTTTCGCAA AAAATGTGTT
0061 CGTGGTCATC AACAGGTTCC TGCAACAGTT GTCNGAACCG GATGCCAAGG ATTTCGAGGA
0121 GACGGCGGCG ATGATTGGAC GACTAATGGC TCGCCGGAAG AACTCCTTCC GCAATATGCC
0181 GGGATTTCGT TCCGTCTGCA AACTTAATGC TGCACTGTGC CGCNCTgcTG CGCTTGGATT
0241 TGGCCAGGGA TCTGAAAAAC TTTCGCGGTG TGCTGCCGGA TGTGTGCGAT GAAGATTTgG
0301 GCGGANNCAT GCCCACGNGA AGCAGCTTTG AGTTTATTTT GGTGCGTCTC CTGGGTTTTT
0361 ATCATCTGCA CGAGCGGATC AGGGAATgCT GCCTCAGTGc AGCCGCATAC TTCACTCAAC
0421 TCCTGCGCAA CAACTTCTTC ATGGACTTCA CCACGcTGCT AATCGCTGCC ATTGCCAAGA
0481 TTAGCAAACT GAGCTNCTTG C |
| in silico PCR Fragment |
0001 GAAACAACCT CCGTTGGTGA CGCTGCTGGT GAAGGACACG GGTTTCGCAA AAAATGTGTT
0061 CGTGGTCATC AACAGGTTCC TGCAACAGTT GTCCGAACCG GATGCCAAGG ATTTCGAGGA
0121 GACGGCGGCG ATGATTGGAC GACTAATGGC TCGCCGGAAG AACTCCTTCC GCAATATGCC
0181 GGGATTTCGT TCCGTCTGCA AACTTAATGC GGCACTGTGC CGC-CTGCTG CGCTTGGATT
0241 TGGCCAGGGA TCTGAAAAAC TTTCGCGGTG TGCTGCCGGA TGTGTGCGAT GAAGATTTGG
0301 GCGGAGCCAT GCCCACGCGA AGCAGCTTTG AGTTTATTTT GGTGCGTCTC CTGGGTTTTT
0361 ATCATCTGCA CGAGCGGATC AGGGAATGCT GCCTCAGTGC AGCCGCATAC TTCACTCAAC
0421 TCCTGCGCAA CAACTTCTTC ATGGACTTCA CCACGCTGCT AATCGCTGCC ATTGCCAAGA
0481 TTAGCAAACT GAGCTCCTTG C |
| Assemble Data |
<PRE>
14174R-2.IR_full 1 AAACAACCTCCGTTGGTGACGCTGCTGGTGAAGGACACGGGTTTCGCAAAAAATGTGTT 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-2.in silico 1 AAACAACCTCCGTTGGTGACGCTGCTGGTGAAGGACACGGGTTTCGCAAAAAATGTGTT 59
14174R-2.IR_full 61 CGTGGTCATCAACAGGTTCCTGCAACAGTTGTC<span class="snp"><tt>N</tt></span>GAACCGGATGCCAAGGATTTCGAGGA 119
|||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||
14174R-2.in silico 61 CGTGGTCATCAACAGGTTCCTGCAACAGTTGTC<span class="snp"><tt>C</tt></span>GAACCGGATGCCAAGGATTTCGAGGA 119
14174R-2.IR_full 121 GACGGCGGCGATGATTGGACGACTAATGGCTCGCCGGAAGAACTCCTTCCGCAATATGCC 179
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-2.in silico 121 GACGGCGGCGATGATTGGACGACTAATGGCTCGCCGGAAGAACTCCTTCCGCAATATGCC 179
14174R-2.IR_full 181 GGGATTTCGTTCCGTCTGCAAACTTAATGC<span class="snp"><tt>T</tt></span>GCACTGTGCCGC<span class="snp"><tt>N</tt></span>CTGCTGCGCTTGGATT 239
||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||
14174R-2.in silico 181 GGGATTTCGTTCCGTCTGCAAACTTAATGC<span class="snp"><tt>G</tt></span>GCACTGTGCCGC<span class="snp"><tt>-</tt></span>CTGCTGCGCTTGGATT 239
14174R-2.IR_full 241 TGGCCAGGGATCTGAAAAACTTTCGCGGTGTGCTGCCGGATGTGTGCGATGAAGATTTGG 299
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-2.in silico 241 TGGCCAGGGATCTGAAAAACTTTCGCGGTGTGCTGCCGGATGTGTGCGATGAAGATTTGG 299
14174R-2.IR_full 301 GCGGA<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>CATGCCCACG<span class="snp"><tt>N</tt></span>GAAGCAGCTTTGAGTTTATTTTGGTGCGTCTCCTGGGTTTTT 359
|||||<span class="snp"><tt> </tt></span><span class="snp"><tt> </tt></span>||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||
14174R-2.in silico 301 GCGGA<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>CATGCCCACG<span class="snp"><tt>C</tt></span>GAAGCAGCTTTGAGTTTATTTTGGTGCGTCTCCTGGGTTTTT 359
14174R-2.IR_full 361 ATCATCTGCACGAGCGGATCAGGGAATGCTGCCTCAGTGCAGCCGCATACTTCACTCAAC 419
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-2.in silico 361 ATCATCTGCACGAGCGGATCAGGGAATGCTGCCTCAGTGCAGCCGCATACTTCACTCAAC 419
14174R-2.IR_full 421 TCCTGCGCAACAACTTCTTCATGGACTTCACCACGCTGCTAATCGCTGCCATTGCCAAGA 479
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-2.in silico 421 TCCTGCGCAACAACTTCTTCATGGACTTCACCACGCTGCTAATCGCTGCCATTGCCAAGA 479
14174R-2.IR_full 481 TTAGCAAACTGAGCT<span class="snp"><tt>N</tt></span>CTTGC 500
|||||||||||||||<span class="snp"><tt> </tt></span>|||||
14174R-2.in silico 481 TTAGCAAACTGAGCT<span class="snp"><tt>C</tt></span>CTTGC 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_140094.1 |
CG14174-RA (CG14174), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_206289.1 |
CG33278-RA (CG33278), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_144348.2 |
CG12135-RA (c12.1), mRNA |
| 0 |
0 |
3 |
2 |
8 |
NM_137155.2 |
CG10228-RA (Pcf11), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_167435.2 |
CG6146-RB, transcript variant B (Top1), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_001014742.1 |
CG6146-RC, transcript variant C (Top1), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_078606.3 |
CG6146-RA, transcript variant A (Top1), mRNA |
| 0 |
0 |
2 |
3 |
19 |
NM_001032051.1 |
CG33715-RD, transcript variant D (Msp-300), mRNA |
| 0 |
0 |
2 |
3 |
17 |
NM_001032053.1 |
CG33715-RB, transcript variant B (Msp-300), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_165329.1 |
CG10947-RC, transcript variant C (CG10947), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_136187.2 |
CG10947-RA, transcript variant A (CG10947), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_165328.1 |
CG10947-RB, transcript variant B (CG10947), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_140000.1 |
CG13310-RA (CG13310), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_143415.2 |
CG1709-RE, transcript variant E (Vha100-1), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_170392.1 |
CG1709-RA, transcript variant A (Vha100-1), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_170396.1 |
CG1709-RD, transcript variant D (Vha100-1), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_136354.1 |
CG7856-RA (CG7856), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137276.2 |
CG6251-RA (Nup62), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142602.2 |
CG4424-RA (CG4424), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_078541.3 |
CG9022-RA (Ost48), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_135207.2 |
CG11319-RA (CG11319), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_140445.1 |
CG3868-RA (CG3868), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014684.1 |
CG7921-RB, transcript variant B (Mgat2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_143506.3 |
CG7921-RA, transcript variant A (Mgat2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_058079.2 |
CG3159-RA, transcript variant A (Eaat2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_205887.1 |
CG3159-RB, transcript variant B (Eaat2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001038976.1 |
CG4637-RB, transcript variant B (hh), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132066.1 |
CG4320-RA (raptor), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079735.3 |
CG4637-RA, transcript variant A (hh), mRNA |
| 0 |
0 |
1 |
1 |
6 |
NM_169704.1 |
CG32855-RA (CG32855), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| + |
|
|
Caspase-3 signal
| N |
|
| ++ |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| ++ |
|
| VB |
|
| ++ |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
