NIG-Fly - Fly Stocks of National Institute of Genetics -

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

14174R-1

Symbol

CG14174

Full Name

CG14174

CG No

CG14174

Old CG No

CG14174

Synonyms

CG14174

Accession No (Link to NCBI)

NM_140094.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGAAACAACCTCCGTTGGTGAC

Primer Seq. 3'

aatctagaggtaccGCAAGGAGCTCAGTTTGCTAA

Predicted Fragment Size

IR fragment full Seq

0001 NAAACAACCT CCGTTGGTGA CGCTGCTGGT GAAGGACACG GGTTTCGCAA AAAATGTGTT 
0061 CGTGGTCATC AACAGGTTCC TGCAACAGTT GTCNGAACCG GATGCCAAGG ATTTCGAGGA 
0121 GACGGCGGCG ATGATTGGAC GACTAATGGC TCGCCGGAAG AACTCCTTCC GCAATATGCC 
0181 GGGATTTCGT TCCGTCTGCA AACTTAATGC TGCACTGTGC CGCNCTgcTG CGCTTGGATT 
0241 TGGCCAGGGA TCTGAAAAAC TTTCGCGGTG TGCTGCCGGA TGTGTGCGAT GAAGATTTgG 
0301 GCGGANNCAT GCCCACGNGA AGCAGCTTTG AGTTTATTTT GGTGCGTCTC CTGGGTTTTT 
0361 ATCATCTGCA CGAGCGGATC AGGGAATgCT GCCTCAGTGc AGCCGCATAC TTCACTCAAC 
0421 TCCTGCGCAA CAACTTCTTC ATGGACTTCA CCACGcTGCT AATCGCTGCC ATTGCCAAGA 
0481 TTAGCAAACT GAGCTNCTTG C

in silico PCR Fragment

0001 GAAACAACCT CCGTTGGTGA CGCTGCTGGT GAAGGACACG GGTTTCGCAA AAAATGTGTT 
0061 CGTGGTCATC AACAGGTTCC TGCAACAGTT GTCCGAACCG GATGCCAAGG ATTTCGAGGA 
0121 GACGGCGGCG ATGATTGGAC GACTAATGGC TCGCCGGAAG AACTCCTTCC GCAATATGCC 
0181 GGGATTTCGT TCCGTCTGCA AACTTAATGC GGCACTGTGC CGC-CTGCTG CGCTTGGATT 
0241 TGGCCAGGGA TCTGAAAAAC TTTCGCGGTG TGCTGCCGGA TGTGTGCGAT GAAGATTTGG 
0301 GCGGAGCCAT GCCCACGCGA AGCAGCTTTG AGTTTATTTT GGTGCGTCTC CTGGGTTTTT 
0361 ATCATCTGCA CGAGCGGATC AGGGAATGCT GCCTCAGTGC AGCCGCATAC TTCACTCAAC 
0421 TCCTGCGCAA CAACTTCTTC ATGGACTTCA CCACGCTGCT AATCGCTGCC ATTGCCAAGA 
0481 TTAGCAAACT GAGCTCCTTG C

Assemble Data

<PRE>

14174R-1.IR_full        1   AAACAACCTCCGTTGGTGACGCTGCTGGTGAAGGACACGGGTTTCGCAAAAAATGTGTT 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-1.in silico      1   AAACAACCTCCGTTGGTGACGCTGCTGGTGAAGGACACGGGTTTCGCAAAAAATGTGTT 59


14174R-1.IR_full       61  CGTGGTCATCAACAGGTTCCTGCAACAGTTGTC<span class="snp"><tt>N</tt></span>GAACCGGATGCCAAGGATTTCGAGGA 119
                           |||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||
14174R-1.in silico     61  CGTGGTCATCAACAGGTTCCTGCAACAGTTGTC<span class="snp"><tt>C</tt></span>GAACCGGATGCCAAGGATTTCGAGGA 119


14174R-1.IR_full       121 GACGGCGGCGATGATTGGACGACTAATGGCTCGCCGGAAGAACTCCTTCCGCAATATGCC 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-1.in silico     121 GACGGCGGCGATGATTGGACGACTAATGGCTCGCCGGAAGAACTCCTTCCGCAATATGCC 179


14174R-1.IR_full       181 GGGATTTCGTTCCGTCTGCAAACTTAATGC<span class="snp"><tt>T</tt></span>GCACTGTGCCGC<span class="snp"><tt>N</tt></span>CTGCTGCGCTTGGATT 239
                           ||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||
14174R-1.in silico     181 GGGATTTCGTTCCGTCTGCAAACTTAATGC<span class="snp"><tt>G</tt></span>GCACTGTGCCGC<span class="snp"><tt>-</tt></span>CTGCTGCGCTTGGATT 239


14174R-1.IR_full       241 TGGCCAGGGATCTGAAAAACTTTCGCGGTGTGCTGCCGGATGTGTGCGATGAAGATTTGG 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-1.in silico     241 TGGCCAGGGATCTGAAAAACTTTCGCGGTGTGCTGCCGGATGTGTGCGATGAAGATTTGG 299


14174R-1.IR_full       301 GCGGA<span class="snp"><tt>N</tt></span><span class="snp"><tt>N</tt></span>CATGCCCACG<span class="snp"><tt>N</tt></span>GAAGCAGCTTTGAGTTTATTTTGGTGCGTCTCCTGGGTTTTT 359
                           |||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
14174R-1.in silico     301 GCGGA<span class="snp"><tt>G</tt></span><span class="snp"><tt>C</tt></span>CATGCCCACG<span class="snp"><tt>C</tt></span>GAAGCAGCTTTGAGTTTATTTTGGTGCGTCTCCTGGGTTTTT 359


14174R-1.IR_full       361 ATCATCTGCACGAGCGGATCAGGGAATGCTGCCTCAGTGCAGCCGCATACTTCACTCAAC 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-1.in silico     361 ATCATCTGCACGAGCGGATCAGGGAATGCTGCCTCAGTGCAGCCGCATACTTCACTCAAC 419


14174R-1.IR_full       421 TCCTGCGCAACAACTTCTTCATGGACTTCACCACGCTGCTAATCGCTGCCATTGCCAAGA 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14174R-1.in silico     421 TCCTGCGCAACAACTTCTTCATGGACTTCACCACGCTGCTAATCGCTGCCATTGCCAAGA 479


14174R-1.IR_full       481 TTAGCAAACTGAGCT<span class="snp"><tt>N</tt></span>CTTGC 500
                           |||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||
14174R-1.in silico     481 TTAGCAAACTGAGCT<span class="snp"><tt>C</tt></span>CTTGC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	1	NM_140094.1	CG14174-RA (CG14174), mRNA
0	0	3	3	3	NM_206289.1	CG33278-RA (CG33278), mRNA
0	0	3	3	2	NM_144348.2	CG12135-RA (c12.1), mRNA
0	0	3	2	8	NM_137155.2	CG10228-RA (Pcf11), mRNA
0	0	3	2	4	NM_167435.2	CG6146-RB, transcript variant B (Top1), mRNA
0	0	3	2	4	NM_001014742.1	CG6146-RC, transcript variant C (Top1), mRNA
0	0	3	2	4	NM_078606.3	CG6146-RA, transcript variant A (Top1), mRNA
0	0	2	3	19	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	2	3	17	NM_001032053.1	CG33715-RB, transcript variant B (Msp-300), mRNA
0	0	2	3	3	NM_165329.1	CG10947-RC, transcript variant C (CG10947), mRNA
0	0	2	3	3	NM_136187.2	CG10947-RA, transcript variant A (CG10947), mRNA
0	0	2	3	3	NM_165328.1	CG10947-RB, transcript variant B (CG10947), mRNA
0	0	1	5	4	NM_140000.1	CG13310-RA (CG13310), mRNA
0	0	1	4	4	NM_143415.2	CG1709-RE, transcript variant E (Vha100-1), mRNA
0	0	1	4	4	NM_170392.1	CG1709-RA, transcript variant A (Vha100-1), mRNA
0	0	1	4	4	NM_170396.1	CG1709-RD, transcript variant D (Vha100-1), mRNA
0	0	1	3	8	NM_136354.1	CG7856-RA (CG7856), mRNA
0	0	1	3	4	NM_137276.2	CG6251-RA (Nup62), mRNA
0	0	1	3	2	NM_142602.2	CG4424-RA (CG4424), mRNA
0	0	1	3	2	NM_078541.3	CG9022-RA (Ost48), mRNA
0	0	1	2	9	NM_135207.2	CG11319-RA (CG11319), mRNA
0	0	1	2	7	NM_140445.1	CG3868-RA (CG3868), mRNA
0	0	1	2	4	NM_001014684.1	CG7921-RB, transcript variant B (Mgat2), mRNA
0	0	1	2	4	NM_143506.3	CG7921-RA, transcript variant A (Mgat2), mRNA
0	0	1	2	3	NM_058079.2	CG3159-RA, transcript variant A (Eaat2), mRNA
0	0	1	2	3	NM_205887.1	CG3159-RB, transcript variant B (Eaat2), mRNA
0	0	1	2	2	NM_001038976.1	CG4637-RB, transcript variant B (hh), mRNA
0	0	1	2	2	NM_132066.1	CG4320-RA (raptor), mRNA
0	0	1	2	2	NM_079735.3	CG4637-RA, transcript variant A (hh), mRNA
0	0	1	1	6	NM_169704.1	CG32855-RA (CG32855), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

Back