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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14133Ra-2

Symbol Pldn Full Name Pallidin

CG No CG14133 Old CG No CG14133

Synonyms CG14133,NP_648494,Pallidin,pallidin,Pldn,pldn,Pallidin ortholog

Accession No (Link to NCBI) NM_140237.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGCCAGTCTAGCCGCTTTA

Primer Seq. 3'

aatctagaggtaccTGCCAATGAGCGACTCCTCGT

Predicted Fragment Size

IR fragment full Seq

0001 ATGGCCAGTC TAGCCGCTTT ACAGTTGTCC GCTGGCGTCC TGCAAATAGC GGAGCCTCCA 
0061 CTAAACCATG TGCGCACCCA GCTTAGGGAA TTGATCGGTA GGCAGAACAA AACATACATC 
0121 GATTTGTCCA AGGAGAAATA TAAACTGGAC TGCAGCGAAG TGGCCAGGCT GAATGATATG 
0181 ATGAGCGATG TAAAGCGATA CAAAGATAAG CTAACAAAAA TCAAGAAGGA AATGCAGGGC 
0241 GTCTATCAGC GCACCAAAGA GTTAAAGAAA CGAGCNCTGC TAACGAGGCG NCCTGNCAAG 
0301 CAGCGTGACT ACCAGCGGAA ACTCGAGAGG CTACAGCACG AGGAGTCGCT CATTGGCA

in silico PCR Fragment

0001 ATGGCCAGTC TAGCCGCTTT ACAGTTGTCC GCTGGCGTCC TGCAAATAGC GGAGCCTCCA 
0061 CTAAACCATG TGCGCACCCA GCTTAGGGAA TTGATCGGTA GGCAGAACAA AACATACATC 
0121 GATTTGTCCA AGGAGAAATA TAAACTGGAC TGCAGCGAAG TGGCCAGGCT GAATGATATG 
0181 ATGAGCGATG TAAAGCGATA CAAAGATAAG CTAACAAAAA TCAAGAAGGA AATGCAGGGC 
0241 GTCTATCAGC GCACCAAAGA GTTAAAGAAA CGAGCCGCTA ACGTGGCGGC CTGCAAGCAG 
0301 CGTGACTACC AGCGGAAACT CGAGAGGCTA CAGCACGAGG AGTCGCTCAT TGGCA

Assemble Data


14133Ra-2.IR full       1   ATGGCCAGTCTAGCCGCTTTACAGTTGTCCGCTGGCGTCCTGCAAATAGCGGAGCCTCCA 60
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14133Ra-2.in silico     1   ATGGCCAGTCTAGCCGCTTTACAGTTGTCCGCTGGCGTCCTGCAAATAGCGGAGCCTCCA 60


14133Ra-2.IR full       61  CTAAACCATGTGCGCACCCAGCTTAGGGAATTGATCGGTAGGCAGAACAAAACATACATC 120
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14133Ra-2.in silico     61  CTAAACCATGTGCGCACCCAGCTTAGGGAATTGATCGGTAGGCAGAACAAAACATACATC 120


14133Ra-2.IR full       121 GATTTGTCCAAGGAGAAATATAAACTGGACTGCAGCGAAGTGGCCAGGCTGAATGATATG 180
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14133Ra-2.in silico     121 GATTTGTCCAAGGAGAAATATAAACTGGACTGCAGCGAAGTGGCCAGGCTGAATGATATG 180


14133Ra-2.IR full       181 ATGAGCGATGTAAAGCGATACAAAGATAAGCTAACAAAAATCAAGAAGGAAATGCAGGGC 240
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14133Ra-2.in silico     181 ATGAGCGATGTAAAGCGATACAAAGATAAGCTAACAAAAATCAAGAAGGAAATGCAGGGC 240


14133Ra-2.IR full       241 GTCTATCAGCGCACCAAAGAGTTAAAGAAACGAGCNCTGCTAACGAGGCGNCCTGNCAAG 300
                            |||||||||||||||||||||||||||||||||||   ||||||| |||| ||||  |||
14133Ra-2.in silico     241 GTCTATCAGCGCACCAAAGAGTTAAAGAAACGAGCC--GCTAACGTGGCGGCCTGC-AAG 300


14133Ra-2.IR full       301 CAGCGTGACTACCAGCGGAAACTCGAGAGGCTACAGCACGAGGAGTCGCTCATTGGCA 358
                            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14133Ra-2.in silico     301 CAGCGTGACTACCAGCGGAAACTCGAGAGGCTACAGCACGAGGAGTCGCTCATTGGCA 358

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	337	0	0	2	NM_140237.1	CG14133-RA (CG14133), mRNA
0.29	1	4	2	2	NM_206649.1	CG2258-RB, transcript variant B (CG2258), mRNA
0.29	1	2	2	6	NM_164521.1	CG3542-RB, transcript variant B (CG3542), mRNA
0.29	1	2	2	6	NM_134894.2	CG3542-RA, transcript variant A (CG3542), mRNA
0	0	4	4	6	NM_080254.2	CG6392-RA (cmet), mRNA
0	0	3	2	2	NM_132426.1	CG15209-RA (CG15209), mRNA
0	0	1	3	6	NM_079345.2	CG4994-RA, transcript variant A (Mpcp), mRNA
0	0	1	2	2	NM_078691.2	CG12532-RA (Bap), mRNA
0	0	0	4	4	NM_170509.1	CG2246-RF, transcript variant F (CG2246), mRNA
0	0	0	4	4	NM_143556.2	CG2246-RE, transcript variant E (CG2246), mRNA
0	0	0	4	4	NM_137543.2	CG15106-RA (Jheh3), mRNA
0	0	0	4	4	NM_170508.1	CG2246-RD, transcript variant D (CG2246), mRNA
0	0	0	4	4	NM_079741.2	CG10210-RA (tst), mRNA
0	0	0	4	4	NM_170507.1	CG2246-RB, transcript variant B (CG2246), mRNA
0	0	0	4	4	NM_170510.1	CG2246-RA, transcript variant A (CG2246), mRNA
0	0	0	4	4	NM_170511.1	CG2246-RC, transcript variant C (CG2246), mRNA
0	0	0	3	7	NM_165034.1	CG6043-RA, transcript variant A (CG6043), mRNA
0	0	0	3	7	NM_165037.1	CG6043-RC, transcript variant C (CG6043), mRNA
0	0	0	3	7	NM_165035.1	CG6043-RB, transcript variant B (CG6043), mRNA
0	0	0	3	7	NM_135785.1	CG6043-RD, transcript variant D (CG6043), mRNA
0	0	0	3	4	NM_057349.4	CG31349-RB, transcript variant B (pyd), mRNA
0	0	0	3	4	NM_176426.2	CG31349-RF, transcript variant F (pyd), mRNA
0	0	0	3	4	NM_169245.2	CG31349-RA, transcript variant A (pyd), mRNA
0	0	0	3	4	NM_169246.2	CG31349-RC, transcript variant C (pyd), mRNA
0	0	0	3	3	NM_167491.1	CG3525-RC, transcript variant C (eas), mRNA
0	0	0	3	3	NM_132511.1	CG2444-RA (CG2444), mRNA
0	0	0	3	3	NM_001043127.1	CG34121-RA (CG34121), mRNA
0	0	0	3	3	NM_176741.1	CG3525-RE, transcript variant E (eas), mRNA
0	0	0	3	3	NM_167490.1	CG3525-RB, transcript variant B (eas), mRNA
0	0	0	3	3	NM_078640.2	CG3525-RA, transcript variant A (eas), mRNA

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