NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13999R-2

Symbol CG13999 Full Name CG13999

CG No CG13999 Old CG No CG13999

Synonyms CG13999

Accession No (Link to NCBI) NM_135128.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAGCTAACACTGCGACGCTACT

Primer Seq. 3'

aatctagaggtaccCTTATCGCCCAGCTGATCTGC

Predicted Fragment Size

IR fragment full Seq

0001 AGCTAACACT GCGACgNTAC TCCTGGGTGC TCCTGTTCCA GTTCGCCCTT TTGGGAGTGG 
0061 ACCTCTTTTG CAACGCCTTT GGTCCTTCGC TGGCCAGAAA CCGATTGCAG ACAGCCATAA 
0121 TTCTCTTTGT CACTCAGGAT GCCCTGATTA TCGCGGAGTA CCTGCTGTTC ACCCTCGCCC 
0181 TGCACTCGAC GTGTGTGTAC CAGGTCGGCG CCTCGCACAT AATNCTGCGG AATTGCAAGC 
0241 TCTTCATGGC CAGCATTACC ATCTACTTCC TTCTGTCCGC CTCCCAGCAC TTTTGGATCA 
0301 TCTACCAATA CCGTCAGCCG CCAGAAGAGG ACGGTCATCA TTGGCCACTG GGTCTGATTG 
0361 CCCTATCAGT GGCACAGCGC ATCATGTCGG TTTTCTATTA CTACAGCTCC AAGTCGACGG 
0421 CTCTGACCAT GGNCAGATCC TCGCTTCAAG GAGGAGCATC TTNGACTGGA TCGCAGATCA 
0481 GCTGGGCGAT AAG

in silico PCR Fragment

0001 AGCTAACACT GCGACGCTAC TCCTGGGTGC TCCTGTTCCA GTTCGCCCTT TTGGGAGTGG 
0061 ACCTCTTTTG CAACGCCTTT GGTCCTTCGC TGGCCAGAAA CCGATTGCAG ACAGCCATAA 
0121 TTCTCTTTGT CACTCAGGAT GCCCTGATTA TCGCGGAGTA CCTGCTGTTC ACCCTCGCCC 
0181 TGCACTCGAC GTGTGTGTAC CAGGTCGGCG CCTCGCACAT AATCCTGCGG AATTGCAAGC 
0241 TCTTCATGGC CAGCATTACC ATCTACTTCC TTCTGTCCGC CTCCCAGCAC TTTTGGATCA 
0301 TCTACCAATA CCGTCAGCCG CCAGAAGAGG ACGGTCATCA TTGGCCACTG GGTCTGATTG 
0361 CCCTATCAGT GGCACAGCGC ATCATGTCGG TTTTCTATTA CTACAGCTCC AAGTCGACGG 
0421 CTCTGACCAT GG-CAGATCC TCGCTTCAAG GAGGAGCATC TT-GACTGGA TCGCAGATCA 
0481 GCTGGGCGAT AAG

Assemble Data


13999R-2.IR_full       1   AGCTAACACTGCGACGNTACTCCTGGGTGCTCCTGTTCCAGTTCGCCCTTTTGGGAGTGG 60
                           |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
13999R-2.in silico     1   AGCTAACACTGCGACGCTACTCCTGGGTGCTCCTGTTCCAGTTCGCCCTTTTGGGAGTGG 60


13999R-2.IR_full       61  ACCTCTTTTGCAACGCCTTTGGTCCTTCGCTGGCCAGAAACCGATTGCAGACAGCCATAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13999R-2.in silico     61  ACCTCTTTTGCAACGCCTTTGGTCCTTCGCTGGCCAGAAACCGATTGCAGACAGCCATAA 120


13999R-2.IR_full       121 TTCTCTTTGTCACTCAGGATGCCCTGATTATCGCGGAGTACCTGCTGTTCACCCTCGCCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13999R-2.in silico     121 TTCTCTTTGTCACTCAGGATGCCCTGATTATCGCGGAGTACCTGCTGTTCACCCTCGCCC 180


13999R-2.IR_full       181 TGCACTCGACGTGTGTGTACCAGGTCGGCGCCTCGCACATAATNCTGCGGAATTGCAAGC 240
                           ||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
13999R-2.in silico     181 TGCACTCGACGTGTGTGTACCAGGTCGGCGCCTCGCACATAATCCTGCGGAATTGCAAGC 240


13999R-2.IR_full       241 TCTTCATGGCCAGCATTACCATCTACTTCCTTCTGTCCGCCTCCCAGCACTTTTGGATCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13999R-2.in silico     241 TCTTCATGGCCAGCATTACCATCTACTTCCTTCTGTCCGCCTCCCAGCACTTTTGGATCA 300


13999R-2.IR_full       301 TCTACCAATACCGTCAGCCGCCAGAAGAGGACGGTCATCATTGGCCACTGGGTCTGATTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13999R-2.in silico     301 TCTACCAATACCGTCAGCCGCCAGAAGAGGACGGTCATCATTGGCCACTGGGTCTGATTG 360


13999R-2.IR_full       361 CCCTATCAGTGGCACAGCGCATCATGTCGGTTTTCTATTACTACAGCTCCAAGTCGACGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13999R-2.in silico     361 CCCTATCAGTGGCACAGCGCATCATGTCGGTTTTCTATTACTACAGCTCCAAGTCGACGG 420


13999R-2.IR_full       421 CTCTGACCATGGNCAGATCCTCGCTTCAAGGAGGAGCATCTTNGACTGGATCGCAGATCA 480
                           |||||||||||| ||||||||||||||||||||||||||||| |||||||||||||||||
13999R-2.in silico     421 CTCTGACCATGG-CAGATCCTCGCTTCAAGGAGGAGCATCTT-GACTGGATCGCAGATCA 480


13999R-2.IR_full       481 GCTGGGCGATAAG 493
                           |||||||||||||
13999R-2.in silico     481 GCTGGGCGATAAG 493

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	473	0	0	0	NM_135128.1	CG13999-RA (CG13999), mRNA
0.21	1	2	2	2	NM_167164.2	CG12737-RB, transcript variant B (Crag), mRNA
0.21	1	2	2	2	NM_080341.2	CG12737-RA, transcript variant A (Crag), mRNA
0	0	4	3	6	NM_130730.1	CG2941-RA (CG2941), mRNA
0	0	2	5	2	NM_167026.2	CG6824-RB, transcript variant B (ovo), mRNA
0	0	2	3	2	NM_131964.1	CG15470-RA (CG15470), mRNA
0	0	1	4	6	NM_169826.1	CG31224-RA (CG31224), mRNA
0	0	1	4	4	NM_140627.2	CG4784-RA (CG4784), mRNA
0	0	1	3	2	NM_135618.1	CG17137-RA (Porin2), mRNA
0	0	1	3	2	NM_057802.4	CG12390-RA (dare), mRNA
0	0	1	2	4	NM_167230.1	CG32683-RA (CG32683), mRNA
0	0	1	2	3	NM_134761.2	CG10869-RA (CG10869), mRNA
0	0	1	2	2	NM_057520.3	CG3497-RA (Su(H)), mRNA
0	0	0	7	7	NM_142990.2	CG6432-RA (CG6432), mRNA
0	0	0	4	5	NM_135575.1	CG18301-RA (CG18301), mRNA
0	0	0	4	4	NM_143288.2	CG31064-RE, transcript variant E (CG31064), mRNA
0	0	0	4	4	NM_170325.1	CG31064-RA, transcript variant A (CG31064), mRNA
0	0	0	4	4	NM_170324.1	CG31064-RB, transcript variant B (CG31064), mRNA
0	0	0	4	2	NM_001015220.1	CG41141-PA (CG41141), mRNA
0	0	0	4	2	NM_132116.1	CG14441-RA (CG14441), mRNA
0	0	0	3	9	NM_001038946.1	CG33969-RB, transcript variant B (CG33969), mRNA
0	0	0	3	9	NM_080312.2	CG7803-RA, transcript variant A (z), mRNA
0	0	0	3	9	NM_001038738.1	CG7803-RB, transcript variant B (z), mRNA
0	0	0	3	8	NM_138243.2	CG9128-RA (Sac1), mRNA
0	0	0	3	7	NM_168852.2	CG5725-RD, transcript variant D (fbl), mRNA
0	0	0	3	7	NM_168851.2	CG5725-RC, transcript variant C (fbl), mRNA
0	0	0	3	7	NM_138997.2	CG5725-RA, transcript variant A (fbl), mRNA
0	0	0	3	7	NM_080131.4	CG5725-RE, transcript variant E (fbl), mRNA
0	0	0	3	7	NM_133434.3	CG5725-RB, transcript variant B (fbl), mRNA
0	0	0	3	5	NM_176447.1	CG33208-RF, transcript variant F (MICAL), mRNA

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