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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13995R-3 |
| Symbol |
CG13995 |
Full Name |
CG13995 |
| CG No |
CG13995 |
Old CG No |
CG13995 |
| Synonyms |
anon-WO0170980.170, anon-WO0170980.169, CG13995 |
| Accession No (Link to NCBI) |
NM_135142.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Yano H, Yamamoto-Hino M, Awano W, Aoki-Kinoshita KF, Tsuda-Sakurai K, Okano H, Goto S.
Identification of proteasome components required for apical localization of Chaoptin using functional genomics.
J Neurogenet (2012) 26(1) 53-63
[
PubMed ID = 22417167
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAGGGACAATCTGCAGCAGTGG |
| Primer Seq. 3' |
aatctagaggtaccCGGTCAAGGGAGAGCATGAAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AGGGACAATC TGCAGCAGTG GTGGGAGAAC TCGTATCGGC GCCAACATCC AGAGCCCACC
0061 GATGACCTCG GCCTGGACTC CGCGGAGCTG NCATCTCGCC CTCCAGGAGC CCAACCAGCT
0121 GCCCGCGGAC TACGACTATG GCAACTTTAG TCTAGGCAAT CCGTATGACG TGGACTCAGA
0181 GCACTCCATT TCCCCGCTGA CGCTGCTCCT GCTCGCTGTT AGCTATGGCC TGGTGGTTTT
0241 CGGCGGCGTC GTGGGCAACT CCACGCTCGT TCTGACCCTC TGCTCCGCCT CTTCGGTGCG
0301 TTTGCGTAAC CCGCTGCTGC TGGCCGTCTG GCATTGCCGA TCTCCTGGTC ACCgGNATNT
0361 NCCGNNCCGG TCACGCTCCT CAATCTCGCG ATGAACCGGA GGNCGNGATC GCTGCCCCTT
0421 GTGCTGTGCA AGGTCATACA cTACGTACAG GTGCATGcCC GTTTCGGCCA GCACTATTTC
0481 GTTTTTCATG CTCTNCCTTG ACCG |
| in silico PCR Fragment |
0001 AGGGACAATC TGCAGCAGTG GTGGGAGAAC TCGTATCGGC GCCAACATCC AGAGCCCACC
0061 GATGACCTCG GCCTGGACTC CGCGGAGCTG -CATCTCGCC CTCCAGGAGC CCAACCAGCT
0121 GCCCGCGGAC TACGACTATG GCAACTTTAG TCTAGGCAAT CCGTATGACG TGGACTCAGA
0181 GCACTCCATT TCCCCGCTGA CGCTGCTCCT GCTCGCTGTT AGCTATGGCC TGGTGGTTTT
0241 CGGCGGCGTC GTGGGCAACT CCACGCTCGT TCTGACCCTC TGCTCCGCCT CTTCGGTGCG
0301 TTTGCGTAAC CCGCTGCTGC TGGCCGTCT- GCATTGCCGA TCTCCTGGTC ACCGGCATCT
0361 -CCGCTCCGG TCACGCTCCT CAATCTCGCG ATGAACCGGA GGACGCGATC GCTGCCCCTT
0421 GTGCTGTGCA AGGTCATACA CTACGTACAG GT-CATGCCC GTTTCGGCCA GCACTATTTC
0481 GTTTTTCATG CTCTCCCTTG ACCG |
| Assemble Data |
13995R-3.IR_full 1 AGGGACAATCTGCAGCAGTGGTGGGAGAACTCGTATCGGCGCCAACATCCAGAGCCCACC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13995R-3.in silico 1 AGGGACAATCTGCAGCAGTGGTGGGAGAACTCGTATCGGCGCCAACATCCAGAGCCCACC 60
13995R-3.IR_full 61 GATGACCTCGGCCTGGACTCCGCGGAGCTGNCATCTCGCCCTCCAGGAGCCCAACCAGCT 120
|||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
13995R-3.in silico 61 GATGACCTCGGCCTGGACTCCGCGGAGCTG-CATCTCGCCCTCCAGGAGCCCAACCAGCT 120
13995R-3.IR_full 121 GCCCGCGGACTACGACTATGGCAACTTTAGTCTAGGCAATCCGTATGACGTGGACTCAGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13995R-3.in silico 121 GCCCGCGGACTACGACTATGGCAACTTTAGTCTAGGCAATCCGTATGACGTGGACTCAGA 180
13995R-3.IR_full 181 GCACTCCATTTCCCCGCTGACGCTGCTCCTGCTCGCTGTTAGCTATGGCCTGGTGGTTTT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13995R-3.in silico 181 GCACTCCATTTCCCCGCTGACGCTGCTCCTGCTCGCTGTTAGCTATGGCCTGGTGGTTTT 240
13995R-3.IR_full 241 CGGCGGCGTCGTGGGCAACTCCACGCTCGTTCTGACCCTCTGCTCCGCCTCTTCGGTGCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13995R-3.in silico 241 CGGCGGCGTCGTGGGCAACTCCACGCTCGTTCTGACCCTCTGCTCCGCCTCTTCGGTGCG 300
13995R-3.IR_full 301 TTTGCGTAACCCGCTGCTGCTGGCCGTCTGGCATTGCCGATCTCCTGGTCACCGGNATNT 360
||||||||||||||||||||||||||||| ||||||||||||||||||||||||| || |
13995R-3.in silico 301 TTTGCGTAACCCGCTGCTGCTGGCCGTCT-GCATTGCCGATCTCCTGGTCACCGGCATCT 360
13995R-3.IR_full 361 CCGNNCCGGTCACGCTCCTCAATCTCGCGATGAACCGGAGGNCGNGATCGCTGCCCCTT 419
||| |||||||||||||||||||||||||||||||||||| || ||||||||||||||
13995R-3.in silico 361 CCGCTCCGGTCACGCTCCTCAATCTCGCGATGAACCGGAGGACGCGATCGCTGCCCCTT 419
13995R-3.IR_full 421 GTGCTGTGCAAGGTCATACACTACGTACAGGTGCATGCCCGTTTCGGCCAGCACTATTTC 479
|||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
13995R-3.in silico 421 GTGCTGTGCAAGGTCATACACTACGTACAGGT-CATGCCCGTTTCGGCCAGCACTATTTC 479
13995R-3.IR_full 481 GTTTTTCATGCTCTNCCTTGACCG 503
|||||||||||||| |||||||||
13995R-3.in silico 481 GTTTTTCATGCTCTCCCTTGACCG 503
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_135142.1 |
CG13995-RA (CG13995), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_001038728.1 |
CG4293-RC, transcript variant C (CG4293), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_130491.3 |
CG4293-RA, transcript variant A (CG4293), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_166847.2 |
CG4293-RB, transcript variant B (CG4293), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_001038727.1 |
CG4293-RD, transcript variant D (CG4293), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_137918.2 |
CG12192-RA (Klp59D), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_001014758.2 |
CG33517-RC, transcript variant C (D2R), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_001031909.1 |
CG33517-RE, transcript variant E (D2R), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_001014760.2 |
CG33517-RA, transcript variant A (D2R), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_001014757.2 |
CG33517-RD, transcript variant D (D2R), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_165694.1 |
CG18345-RB, transcript variant B (trpl), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_165695.1 |
CG18345-RC, transcript variant C (trpl), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_057547.3 |
CG18345-RA, transcript variant A (trpl), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_141919.2 |
CG10038-RA, transcript variant A (CG10038), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_169460.1 |
CG10038-RB, transcript variant B (CG10038), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_001015269.1 |
CG40450-PA.3 (CG40450), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_001015268.1 |
CG40450-PB.3 (CG40450), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_131975.3 |
CG32767-RA, transcript variant A (CG32767), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_206628.1 |
CG32767-RB, transcript variant B (CG32767), mRNA |
| 0 |
0 |
1 |
12 |
4 |
NM_079677.2 |
CG6027-RA (cdi), mRNA |
| 0 |
0 |
1 |
6 |
4 |
NM_140771.2 |
CG5147-RA (CG5147), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_143596.2 |
CG12054-RA (CG12054), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_144350.1 |
CG7487-RA (RecQ4), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_135646.2 |
CG6230-RA (CG6230), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_135688.2 |
CG5202-RB, transcript variant B (escl), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_164979.1 |
CG5202-RA, transcript variant A (escl), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_140990.1 |
CG13253-RA (CG13253), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166484.1 |
CG17952-RC, transcript variant C (LBR), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137764.2 |
CG17952-RA, transcript variant A (LBR), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166485.1 |
CG17952-RB, transcript variant B (LBR), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
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| + |
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| DC |
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| + |
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| DM |
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| + |
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| VM |
 |
| + |
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| VB |
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| |
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| Dcompartment |
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Penetrance |
| + |
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