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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13894R-1

Symbol CG13894 Full Name CG13894

CG No CG13894 Old CG No CG13894

Synonyms dmCG13894, CG13894

Accession No (Link to NCBI) NM_138210.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Bauke AC, Sasse S, Matzat T, Klämbt C.
A transcriptional network controlling glial development in the Drosophila visual system.
Development (2015) 142(12) 2184-93 [ PubMed ID = 26015542 ] [ RRC reference ]

Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52 [ PubMed ID = 22798488 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAATTCGCGTCAGCACCCGAG

Primer Seq. 3'

aatctagaggtaccGTGGCTTCAACTGGTTGCTGG

Predicted Fragment Size

IR fragment full Seq

0001 CAATTCGCGN CAGcaCCCGA GTATGCAGTT CTTTGCGTTT CCCCGGCCGG AGAACCCCTT 
0061 CCACAAGCTG TGGAAGGAGG CGTGCCACGC CTCCCTAAAG ACGAATTGTG CCCTTCAAAA 
0121 AGCCAGTGGT ATGTGCCCTG CACTTTGACC CCAGTGTCTT GGGTGGTAGG CGACTGCAGT 
0181 CGAATGCGCT GCCCACCCTG CGTCTGGAAG TTCCCTCCAA CCTGGAGGCC GTTGAGCAGC 
0241 AGGCCATGGT GGAGGAGATC GAGAGGAGCC GAAAGTGCGC CTACATCAAT GCAGTGGTAT 
0301 ACGAATGGCT AGTAAGGGCC AATATCAATC CCCAGCTGCG AGGTAGCATT ACCCACGGAA 
0361 TGATCAAGGA CAAGGCGGAG AATGCCCGAC AAGTCATAGG GAGCACATCC TTTATAGCAG 
0421 ACAATCGCTG GTTAAACCGT TTCCGAGAGA CTCATCTTca aNGNTTTGCC CAAAAACTAG 
0481 CCAGCAACCA GTTGAAgNCA C

in silico PCR Fragment

0001 CAATTCGCGT CAGCACCCGA GTATGCAGTT CTTTGCGTTT CCCCGGCCGG AGAACCCCTT 
0061 CCACAAGCTG TGGAAGGAGG CGTGCCACGC CTCCCT-AAG ACGAATTGTG CCCTTCAAAA 
0121 AGCCAGTGGT ATGTGCCCTG CACTTTGACC CCAGTGTCTT GGGTGGCAGG CGACTGCAGT 
0181 CGAATGCGCT GCCCACCCTG CGTCTGGAAG TTCCCTCCAA CCTGGAGGCC GTTGAGCAGC 
0241 AGGCCATGGT GGAGGAGATC GAGAGGAGCC GAAAGTGCGC CTACATCAAT GCAGTGGTAT 
0301 ACGAATGGCT AGTAAGGGCC AATATCAATC CCCAGCTGCG AGGTAGCATT ACCCACGGAA 
0361 TGATCAAGGA CAAGGCGGAG AATGCCAGAC AAGTCATAGG GAGCACATCC TTTATAGCAG 
0421 ACAATCGCTG GTTAAACCGT TTCCGAGAGA CTCATCTTCA AGGCTTTGCC CAAAAACTAG 
0481 CCAGCAACCA GTTGAAGCCA C

Assemble Data


13894R-1.IR_full       1   CAATTCGCGNCAGCACCCGAGTATGCAGTTCTTTGCGTTTCCCCGGCCGGAGAACCCCTT 60
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
13894R-1.in silico     1   CAATTCGCGTCAGCACCCGAGTATGCAGTTCTTTGCGTTTCCCCGGCCGGAGAACCCCTT 60


13894R-1.IR_full       61  CCACAAGCTGTGGAAGGAGGCGTGCCACGCCTCCCTAAAGACGAATTGTGCCCTTCAAAA 120
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
13894R-1.in silico     61  CCACAAGCTGTGGAAGGAGGCGTGCCACGCCTCCCT-AAGACGAATTGTGCCCTTCAAAA 120


13894R-1.IR_full       121 AGCCAGTGGTATGTGCCCTGCACTTTGACCCCAGTGTCTTGGGTGGTAGGCGACTGCAGT 180
                           |||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
13894R-1.in silico     121 AGCCAGTGGTATGTGCCCTGCACTTTGACCCCAGTGTCTTGGGTGGCAGGCGACTGCAGT 180


13894R-1.IR_full       181 CGAATGCGCTGCCCACCCTGCGTCTGGAAGTTCCCTCCAACCTGGAGGCCGTTGAGCAGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13894R-1.in silico     181 CGAATGCGCTGCCCACCCTGCGTCTGGAAGTTCCCTCCAACCTGGAGGCCGTTGAGCAGC 240


13894R-1.IR_full       241 AGGCCATGGTGGAGGAGATCGAGAGGAGCCGAAAGTGCGCCTACATCAATGCAGTGGTAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13894R-1.in silico     241 AGGCCATGGTGGAGGAGATCGAGAGGAGCCGAAAGTGCGCCTACATCAATGCAGTGGTAT 300


13894R-1.IR_full       301 ACGAATGGCTAGTAAGGGCCAATATCAATCCCCAGCTGCGAGGTAGCATTACCCACGGAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13894R-1.in silico     301 ACGAATGGCTAGTAAGGGCCAATATCAATCCCCAGCTGCGAGGTAGCATTACCCACGGAA 360


13894R-1.IR_full       361 TGATCAAGGACAAGGCGGAGAATGCCCGACAAGTCATAGGGAGCACATCCTTTATAGCAG 420
                           |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
13894R-1.in silico     361 TGATCAAGGACAAGGCGGAGAATGCCAGACAAGTCATAGGGAGCACATCCTTTATAGCAG 420


13894R-1.IR_full       421 ACAATCGCTGGTTAAACCGTTTCCGAGAGACTCATCTTCAANGNTTTGCCCAAAAACTAG 480
                           ||||||||||||||||||||||||||||||||||||||||| | ||||||||||||||||
13894R-1.in silico     421 ACAATCGCTGGTTAAACCGTTTCCGAGAGACTCATCTTCAAGGCTTTGCCCAAAAACTAG 480


13894R-1.IR_full       481 CCAGCAACCAGTTGAAGNCAC 501
                           ||||||||||||||||| |||
13894R-1.in silico     481 CCAGCAACCAGTTGAAGCCAC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	1	4	5	NM_138210.1	CG13894-RA (CG13894), mRNA
0.41	2	2	2	2	NM_143465.1	CG1973-RA (CG1973), mRNA
0	0	3	3	4	NM_169148.3	CG2336-RA (CG2336), mRNA
0	0	2	5	4	NM_135697.1	CG14945-RA, transcript variant A (CG14945), mRNA
0	0	2	5	4	NM_164987.1	CG14945-RB, transcript variant B (CG14945), mRNA
0	0	2	3	3	NM_142146.1	CG14860-RA (CG14860), mRNA
0	0	1	11	14	NM_057685.3	CG11527-RA (Tig), mRNA
0	0	1	5	2	NM_164863.1	CG31884-RB, transcript variant B (Trx-2), mRNA
0	0	1	5	2	NM_078802.2	CG31884-RA, transcript variant A (Trx-2), mRNA
0	0	1	4	3	NM_166411.1	CG8994-RB, transcript variant B (exu), mRNA
0	0	1	4	3	NM_166410.1	CG8994-RA, transcript variant A (exu), mRNA
0	0	1	4	3	NM_166412.1	CG8994-RC, transcript variant C (exu), mRNA
0	0	1	4	2	NM_142397.1	CG14318-RA (CG14318), mRNA
0	0	1	3	6	NM_142456.1	CG15803-RA (CG15803), mRNA
0	0	1	3	4	NM_134603.1	CG1489-RA (Pros45), mRNA
0	0	1	2	9	NM_140791.2	CG6897-RA (CG6897), mRNA
0	0	1	2	4	NM_135332.2	CG7380-RA (CG7380), mRNA
0	0	0	5	6	NM_139356.1	CG3524-RA (v(2)k05816), mRNA
0	0	0	5	3	NM_140060.1	CG3445-RA (phol), mRNA
0	0	0	4	12	NM_057531.3	CG6863-RA, transcript variant A (tok), mRNA
0	0	0	4	12	NM_170168.1	CG6863-RB, transcript variant B (tok), mRNA
0	0	0	4	8	NM_176734.1	CG33206-RA, transcript variant A (l(1)G0168), mRNA
0	0	0	4	7	NM_133014.2	CG6269-RA (unc-4), mRNA
0	0	0	4	6	NM_135753.2	CG9934-RA (CG9934), mRNA
0	0	0	4	6	NM_176735.1	CG33206-RB, transcript variant B (l(1)G0168), mRNA
0	0	0	4	6	NM_080327.2	CG3665-RA, transcript variant A (Fas2), mRNA
0	0	0	4	5	NM_167005.1	CG3665-RC, transcript variant C (Fas2), mRNA
0	0	0	4	5	NM_167376.1	CG7107-RD, transcript variant D (up), mRNA
0	0	0	4	5	NM_167004.1	CG3665-RB, transcript variant B (Fas2), mRNA
0	0	0	4	3	NM_080349.2	CG7107-RA, transcript variant A (up), mRNA

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