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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13892R-3 
 Symbol Cypl  Full Name Cyclophilin-like 
 CG No CG13892  Old CG No CG13892 
 Synonyms CG13892, Cypl 
 Accession No (Link to NCBI) NM_079150.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAATGCTGGTGGCATTCCCGA 
 Primer Seq. 3' 
aatctagaggtaccTCTCCACCTTGGCCTTGATGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CAATGCTGGT GGCATTCCCG ACAAGGCGTG GCAGCCGCAC TTCGTGACCC TGGAAACGAG 
0061 CATGGGAGAA ATCACCGTGG AGCTGTACTG GAAACACGCG CCCAACACCT GCCGCAACTT 
0121 TGCCGAACTC TCGAGAAGGG GCTACTACAA CAACGTGGTG TTCCACCGGA TCATCAGGGA 
0181 CTTCATGATC CAGGGCGGCG ATCCCACGGG CACTGGCCGC GGAGGCGCCN TCCATCTACG 
0241 GATCGGAGTT CGCGGACGAG CTGCACGGCG ACTTGAGGCA CACGGGCGCG GGCATCCTCT 
0301 CGATGGCCAA CTCTGGACCG GACACCAACG GGTCCCAGTT CTTCATCACG CTGGCGCCCA 
0361 CGCAGTGGCT GGATGGCAAG CACACGATCT TCGGCAGGGT CTACACGGGC ATGGAGGTGG 
0421 TCAAGCGGAT CGGCATGGTG GAAACCGACA AGAACGATCG CCCCGTGGAT CCCCTAAGGA 
0481 TCATCAAGGC CAAGGTGGAG A 
 in silico PCR Fragment 
0001 CAATGCTGGT GGCATTCCCG ACAAGGCGTG GCAGCCGCAC TTCGTGACCC TGGAAACGAG 
0061 CATGGGAGAA ATCACCGTGG AGCTGTACTG GAAACACGCG CCCAACACCT GCCGCAACTT 
0121 TGCCGAACTC TCGAGAAGGG GCTACTACAA CAACGTGGTG TTCCACCGGA TCATCAGGGA 
0181 CTTCATGATC CAGGGCGGCG ATCCCACGGG CACTGGCCGC GGAGGCGCC- TCCATCTACG 
0241 GATCGGAGTT CGCGGACGAG CTGCACGGCG ACTTGAGGCA CACGGGCGCG GGCATCCTCT 
0301 CGATGGCCAA CTCTGGACCG GACACCAACG GGTCCCAGTT CTTCATCACG CTGGCGCCCA 
0361 CGCAGTGGCT GGATGGCAAG CACACGATCT TCGGCAGGGT CTACACGGGC ATGGAGGTGG 
0421 TCAAGCGGAT CGGCATGGTG GAAACCGACA AGAACGATCG CCCCGTGGAT CCCCTAAGGA 
0481 TCATCAAGGC CAAGGTGGAG A 
 Assemble Data 
<PRE>

13892R-3.IR_full       1   CAATGCTGGTGGCATTCCCGACAAGGCGTGGCAGCCGCACTTCGTGACCCTGGAAACGAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13892R-3.in silico     1   CAATGCTGGTGGCATTCCCGACAAGGCGTGGCAGCCGCACTTCGTGACCCTGGAAACGAG 60


13892R-3.IR_full       61  CATGGGAGAAATCACCGTGGAGCTGTACTGGAAACACGCGCCCAACACCTGCCGCAACTT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13892R-3.in silico     61  CATGGGAGAAATCACCGTGGAGCTGTACTGGAAACACGCGCCCAACACCTGCCGCAACTT 120


13892R-3.IR_full       121 TGCCGAACTCTCGAGAAGGGGCTACTACAACAACGTGGTGTTCCACCGGATCATCAGGGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13892R-3.in silico     121 TGCCGAACTCTCGAGAAGGGGCTACTACAACAACGTGGTGTTCCACCGGATCATCAGGGA 180


13892R-3.IR_full       181 CTTCATGATCCAGGGCGGCGATCCCACGGGCACTGGCCGCGGAGGCGCC<span class="snp"><tt>N</tt></span>TCCATCTACG 240
                           |||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||
13892R-3.in silico     181 CTTCATGATCCAGGGCGGCGATCCCACGGGCACTGGCCGCGGAGGCGCC<span class="snp"><tt>-</tt></span>TCCATCTACG 240


13892R-3.IR_full       241 GATCGGAGTTCGCGGACGAGCTGCACGGCGACTTGAGGCACACGGGCGCGGGCATCCTCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13892R-3.in silico     241 GATCGGAGTTCGCGGACGAGCTGCACGGCGACTTGAGGCACACGGGCGCGGGCATCCTCT 300


13892R-3.IR_full       301 CGATGGCCAACTCTGGACCGGACACCAACGGGTCCCAGTTCTTCATCACGCTGGCGCCCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13892R-3.in silico     301 CGATGGCCAACTCTGGACCGGACACCAACGGGTCCCAGTTCTTCATCACGCTGGCGCCCA 360


13892R-3.IR_full       361 CGCAGTGGCTGGATGGCAAGCACACGATCTTCGGCAGGGTCTACACGGGCATGGAGGTGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13892R-3.in silico     361 CGCAGTGGCTGGATGGCAAGCACACGATCTTCGGCAGGGTCTACACGGGCATGGAGGTGG 420


13892R-3.IR_full       421 TCAAGCGGATCGGCATGGTGGAAACCGACAAGAACGATCGCCCCGTGGATCCCCTAAGGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13892R-3.in silico     421 TCAAGCGGATCGGCATGGTGGAAACCGACAAGAACGATCGCCCCGTGGATCCCCTAAGGA 480


13892R-3.IR_full       481 TCATCAAGGCCAAGGTGGAGA 501
                           |||||||||||||||||||||
13892R-3.in silico     481 TCATCAAGGCCAAGGTGGAGA 501

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_079150.2  CG13892-RA (Cypl), mRNA 
1.24   6  6  3  5  NM_136380.1  CG17266-RA (CG17266), mRNA 
0.62   3  11  23  8  NM_137851.2  CG2852-RA, transcript variant A (CG2852), mRNA 
0.62   3  11  23  8  NM_166539.1  CG2852-RB, transcript variant B (CG2852), mRNA 
0.2   1  2  5  6  NM_141889.2  CG3169-RA (Spt3), mRNA 
0.2   1  1  2  1  NM_137714.2  CG9437-RA (CG9437), mRNA 
0   0  4  4  3  NM_143034.3  CG5808-RA (CG5808), mRNA 
0   0  3  17  19  NM_078642.3  CG9916-RA (Cyp1), mRNA 
0   0  3  3  4  NM_134903.2  CG17261-RA (CG17261), mRNA 
0   0  2  4  5  NM_078781.1  CG6641-RA (Pbprp5), mRNA 
0   0  2  4  2  NM_130582.2  CG14798-RA, transcript variant A (CG14798), mRNA 
0   0  2  4  2  NM_166901.1  CG14798-RB, transcript variant B (CG14798), mRNA 
0   0  2  3  7  NM_143380.3  CG1520-RA, transcript variant A (WASp), mRNA 
0   0  2  3  7  NM_170378.2  CG1520-RB, transcript variant B (WASp), mRNA 
0   0  2  3  7  NM_176578.1  CG1520-RC, transcript variant C (WASp), mRNA 
0   0  2  2  8  NM_137617.3  CG11209-RA (ppk6), mRNA 
0   0  2  2  5  NM_143026.1  CG6879-RA (CG6879), mRNA 
0   0  1  6  6  NM_078845.3  CG15274-RA (GABA-B-R1), mRNA 
0   0  1  5  5  NM_143153.2  CG4719-RA (tankyrase), mRNA 
0   0  1  4  4  NR_002098.1  CG4719-RA (tankyrase), mRNA, miscRNA 
0   0  1  4  4  NR_002097.1  CG4719-RA (tankyrase), mRNA, miscRNA, miscRNA 
0   0  1  4  3  NM_080313.2  CG9659-RC, transcript variant C (egh), mRNA 
0   0  1  4  3  NM_166946.1  CG9659-RB, transcript variant B (egh), mRNA 
0   0  1  3  7  NM_206659.1  CG11265-RC, transcript variant C (CG11265), mRNA 
0   0  1  3  7  NM_206660.1  CG11265-RB, transcript variant B (CG11265), mRNA 
0   0  1  3  7  NM_206658.1  CG11265-RE, transcript variant E (CG11265), mRNA 
0   0  1  3  7  NM_132262.2  CG11265-RA, transcript variant A (CG11265), mRNA 
0   0  1  3  4  NM_165572.1  CG30381-RA (CG30381), mRNA 
0   0  1  3  3  NM_164343.1  CG1090-RA, transcript variant A (CG1090), mRNA 
0   0  1  3  3  NM_141202.2  CG1090-RB, transcript variant B (CG1090), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme03040 : Spliceosome

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