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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13879R-2 |
| Symbol |
CG13879 |
Full Name |
CG13879 |
| CG No |
CG13879 |
Old CG No |
CG13879 |
| Synonyms |
CG13879 |
| Accession No (Link to NCBI) |
NM_138176.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Kamimura K, Koyama T, Habuchi H, Ueda R, Masu M, Kimata K, Nakato H.<br> Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling.<br> J Cell Biol (2006) 174(6) 773-8
[
PubMed ID = 16966419
]
[
RRC reference
]
Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.<br> Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.<br> Proc Natl Acad Sci U S A (2006) 103(39) 14417-22
[
PubMed ID = 16971484
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCTAGGCCAGCACTTCGATGA |
| Primer Seq. 3' |
aatctagaggtaccGCGAATCTACCTTCTCGATGA |
| Predicted Fragment Size |
434 |
| IR fragment full Seq |
0001 TCTAGGCCAG CACTTCGATG ACCAGTTAAA GCTGGCTAGC GTGCGAGTTG GGCGATTTCT
0061 CGGACGATGA CCTGGCGCTC CTTGACAAAT GCGCCCAATA TTATTCACTT TTGCACATTC
0121 ACGACATCAA TCTGAACTAC TTGCGCGACT TCTATTGTGC CAAGAAGAGG GAATGCATAG
0181 AAAACCGGCA GACCACAGTG CAGCAACGCG AAGANGCTGC AGCGCATCCT CTCGTCCATC
0241 GAGGAGGCGA CTAGGGACGT AGTTATGTTG GAGAGATTTA ATGCTGCTGC GGAAGAGCGG
0301 CTCATCCCGG ATATCGTCGT AATGCAGCGA AACGCCCAAC AGCTTGCAAC AAAACAGGCC
0361 TTACTGGACC GGCAAAAAAC CCTTAAGATC CCAAAGGATT TTAGCATTGA AAGTGTCATC
0421 GAGAAGGTAG ATTCNC |
| in silico PCR Fragment |
0001 TCTAGGCCAG CACTTCGATG ACCAGTTAAA GCTGGCTAGC GT-CGAGTTG GGCGATTTCT
0061 CGGACGATGA CCTGGCGCTC CTTGACAAAT GCGCCCAATA TTATTCACTT TTGCACATTC
0121 ACGACATCAA TCTGAACTAC TTGCGCGACT TCTATTGTGC CAAGAAGAGG GAATGCATAG
0181 AAAACCGGCA GACCACAGTG CAGCAACGCG TAGA-GCTGC AGCGCATCCT CTCGTCCATC
0241 GAGGAGGCGA CTAGGGACGT AGTTATGTTG GAGAGATTTA ATGCTGCTGC GGAAGAGCGG
0301 CTCATCCCGG ATATCGTCGT AATGCAGCGA AACGCCCAAC AGCTTGCAAC AAAACAGGCC
0361 TTACTGGACC GGCAAAAAAC CCTTAAGATC CCAAAGGATT TTAGCATTGA AAGTGTCATC
0421 GAGAAGGTAG ATTCGC |
| Assemble Data |
<PRE>
13879R-2.IR_full 1 TCTAGGCCAGCACTTCGATGACCAGTTAAAGCTGGCTAGCGT<span class="snp"><tt>G</tt></span>CGAGTTGGGCGATTTCT 60
||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||
13879R-2.in silico 1 TCTAGGCCAGCACTTCGATGACCAGTTAAAGCTGGCTAGCGT<span class="snp"><tt>-</tt></span>CGAGTTGGGCGATTTCT 60
13879R-2.IR_full 61 CGGACGATGACCTGGCGCTCCTTGACAAATGCGCCCAATATTATTCACTTTTGCACATTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13879R-2.in silico 61 CGGACGATGACCTGGCGCTCCTTGACAAATGCGCCCAATATTATTCACTTTTGCACATTC 120
13879R-2.IR_full 121 ACGACATCAATCTGAACTACTTGCGCGACTTCTATTGTGCCAAGAAGAGGGAATGCATAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13879R-2.in silico 121 ACGACATCAATCTGAACTACTTGCGCGACTTCTATTGTGCCAAGAAGAGGGAATGCATAG 180
13879R-2.IR_full 181 AAAACCGGCAGACCACAGTGCAGCAACGCG<span class="snp"><tt>A</tt></span>AGA<span class="snp"><tt>N</tt></span>GCTGCAGCGCATCCTCTCGTCCATC 240
||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||
13879R-2.in silico 181 AAAACCGGCAGACCACAGTGCAGCAACGCG<span class="snp"><tt>T</tt></span>AGA<span class="snp"><tt>-</tt></span>GCTGCAGCGCATCCTCTCGTCCATC 240
13879R-2.IR_full 241 GAGGAGGCGACTAGGGACGTAGTTATGTTGGAGAGATTTAATGCTGCTGCGGAAGAGCGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13879R-2.in silico 241 GAGGAGGCGACTAGGGACGTAGTTATGTTGGAGAGATTTAATGCTGCTGCGGAAGAGCGG 300
13879R-2.IR_full 301 CTCATCCCGGATATCGTCGTAATGCAGCGAAACGCCCAACAGCTTGCAACAAAACAGGCC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13879R-2.in silico 301 CTCATCCCGGATATCGTCGTAATGCAGCGAAACGCCCAACAGCTTGCAACAAAACAGGCC 360
13879R-2.IR_full 361 TTACTGGACCGGCAAAAAACCCTTAAGATCCCAAAGGATTTTAGCATTGAAAGTGTCATC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13879R-2.in silico 361 TTACTGGACCGGCAAAAAACCCTTAAGATCCCAAAGGATTTTAGCATTGAAAGTGTCATC 420
13879R-2.IR_full 421 GAGAAGGTAGATTC<span class="snp"><tt>N</tt></span>C 436
||||||||||||||<span class="snp"><tt> </tt></span>|
13879R-2.in silico 421 GAGAAGGTAGATTC<span class="snp"><tt>G</tt></span>C 436
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
416 |
0 |
0 |
0 |
NM_138176.1 |
CG13879-RA (CG13879), mRNA |
| 0.48 |
2 |
3 |
3 |
3 |
NM_144094.1 |
CG15386-RA (CG15386), mRNA |
| 0.24 |
1 |
3 |
3 |
3 |
NM_143409.1 |
CG11873-RA (CG11873), mRNA |
| 0.24 |
1 |
3 |
2 |
2 |
NM_140936.2 |
CG17147-RA (CG17147), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_142563.1 |
CG16718-RA, transcript variant A (CG16718), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_169867.1 |
CG16718-RB, transcript variant B (CG16718), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_132041.2 |
CG15765-RA (CG15765), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_134508.2 |
CG32528-RA (CG32528), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_001042804.1 |
CG2174-RB, transcript variant B (Myo10A), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_132441.1 |
CG2174-RA, transcript variant A (Myo10A), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_169656.1 |
CG6125-RA, transcript variant A (CG6125), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142208.2 |
CG6125-RB, transcript variant B (CG6125), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_079191.2 |
CG14999-RA (RfC40), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_176243.1 |
CG18375-RB, transcript variant B (CG18375), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137721.1 |
CG18375-RA, transcript variant A (CG18375), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079940.4 |
CG16973-RA, transcript variant A (msn), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206250.2 |
CG16973-RC, transcript variant C (msn), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206252.2 |
CG16973-RB, transcript variant B (msn), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206251.2 |
CG16973-RD, transcript variant D (msn), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206249.2 |
CG16973-RE, transcript variant E (msn), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135294.1 |
CG13795-RA (CG13795), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_142307.1 |
CG12783-RA (CG12783), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_135966.1 |
CG17928-RA (CG17928), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_133061.2 |
CG32549-RD, transcript variant D (CG32549), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_206785.1 |
CG32549-RF, transcript variant F (CG32549), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_206787.1 |
CG32549-RA, transcript variant A (CG32549), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_206786.1 |
CG32549-RB, transcript variant B (CG32549), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167614.1 |
CG32549-RE, transcript variant E (CG32549), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_167615.1 |
CG32549-RC, transcript variant C (CG32549), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_206412.1 |
CG10508-RF, transcript variant F (CG10508), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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