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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
temporarily unavailable
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13780R-2 |
| Symbol |
Pvf2 |
Full Name |
PDGF- and VEGF-related factor 2 |
| CG No |
CG13780 |
Old CG No |
CG13780 |
| Synonyms |
PVF, VEGF-2, PVF2, VEGF, PVFs, pvf2, VEGF27Cb, Vegf27Cb, CG13780, DmVEGF-2, Pvf2, PV2 |
| Accession No (Link to NCBI) |
NM_078775.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Pan X, O'Connor MB.<br> Coordination among multiple receptor tyrosine kinase signals controls Drosophila developmental timing and body size.<br> Cell Rep (2021) 36(9) 109644
[
PubMed ID = 34469735
]
[
RRC reference
]
Lopez-Bellido R, Puig S, Huang PJ, Tsai CR, Turner HN, Galko MJ, Gutstein HB.<br> Growth Factor Signaling Regulates Mechanical Nociception in Flies and Vertebrates.<br> J Neurosci (2019) 39(30) 6012-6030
[
PubMed ID = 31138657
]
[
RRC reference
]
Song W, Kir S, Hong S, Hu Y, Wang X, Binari R, Tang HW, Chung V, Banks AS, Spiegelman B, Perrimon N.<br> Tumor-Derived Ligands Trigger Tumor Growth and Host Wasting via Differential MEK Activation.<br> Dev Cell (2019) 48(2) 277-286.e6
[
PubMed ID = 30639055
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.<br> Identification and functional analysis of healing regulators in Drosophila.<br> PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Wu Y, Brock AR, Wang Y, Fujitani K, Ueda R, Galko MJ.<br> A blood-borne PDGF/VEGF-like ligand initiates wound-induced epidermal cell migration in Drosophila larvae.<br> Curr Biol (2009) 19(17) 1473-7
[
PubMed ID = 19646875
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATTTTCGAAGACGACACACGC |
| Primer Seq. 3' |
aatctagaggtaccTGAGCTCCCTGGTACGATTAT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATTTTCGAAG ACGACACACG CTTATTCCAG TTTTCCAACT GACATAAATA GCAATTACTT
0061 CGACGTGCGG AATTCGACCT TGCCCATGAC CATGACCACC TCCCATCCAC AAACCAATCG
0121 TCCTCTCGCT CGCTACGTTC TCCCCCCAAA CGAGTTGCAT ACCGCGGACG AGGACGAGGA
0181 GTCCTCTGAC TTAGAGCCGT ACTTCGAGGA TCGAGCGGTC CAGCGGGACA GGTCGTATCT
0241 CATCCGATTG ATCCGCCGGC GCGTGCCACG TGATCAGGCC CCCCAGCAAG TAATTCTTCA
0301 GGGCCGCAGT CTCAGTGGCG TTCACTGGGG GCAGGGCGAC GACAATCATC TCAGCTCCGA
0361 TGCGGCCGTC GATGGGAGCG ATGACTATCC ATCGGATCAG CCGGGCGATT TGACAGTGTT
0421 GAAAGAGCGA ATCGCAGAAC AAAACTCCGT TGAAAAGTTG AGGACTATGA AATACAACAA
0481 TAATCGTACC AGGGAGCTCA |
| in silico PCR Fragment |
0001 ATTTTCGAAG ACGACACACG CTTATTCCAG TTTTCCAACT GACATAAATA GCAATTACTT
0061 CGACGTGCGG AATTCGACCT TGCCCATGAC CATGACCACC TCCCATCCAC AAACCAATCG
0121 TCCTCTCGCT CGCTACGTTC TCCCCCCAAA CGAGTTGCAT ACCGCGGACG AGGACGAAGA
0181 GTCCTCTGAC TTAGAGCCGT ACTTTGAGGA TCGAGCGGTC CAGCGGGACA GGTCGTATCT
0241 CATCCGATTG ATCCGCCGGC GCGTGCCACG TGATCAGGCC CCCCAGCAAG TAATTCTTCA
0301 GGGCCGCAGT CTCAGTGGCG TTCACTGGGG GCAGGGCGAC GACAATCATC TCAGCTCCGA
0361 TGCGGCCGTC GATGGGAGCG ATGACTATCC ATCGGATCAG CCGGGCGATT TGACAGTGTT
0421 GAAAGAGCGA ATCGCCGAAC AAAACTCCGT TGAAAAGTTG AGGACTATGA AATACAACAA
0481 TAATCGTACC AGGGAGCTCA |
| Assemble Data |
<PRE>
13780R-2.IR_full 1 ATTTTCGAAGACGACACACGCTTATTCCAGTTTTCCAACTGACATAAATAGCAATTACTT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13780R-2.in silico 1 ATTTTCGAAGACGACACACGCTTATTCCAGTTTTCCAACTGACATAAATAGCAATTACTT 60
13780R-2.IR_full 61 CGACGTGCGGAATTCGACCTTGCCCATGACCATGACCACCTCCCATCCACAAACCAATCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13780R-2.in silico 61 CGACGTGCGGAATTCGACCTTGCCCATGACCATGACCACCTCCCATCCACAAACCAATCG 120
13780R-2.IR_full 121 TCCTCTCGCTCGCTACGTTCTCCCCCCAAACGAGTTGCATACCGCGGACGAGGACGA<span class="snp"><tt>G</tt></span>GA 180
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||
13780R-2.in silico 121 TCCTCTCGCTCGCTACGTTCTCCCCCCAAACGAGTTGCATACCGCGGACGAGGACGA<span class="snp"><tt>A</tt></span>GA 180
13780R-2.IR_full 181 GTCCTCTGACTTAGAGCCGTACTT<span class="snp"><tt>C</tt></span>GAGGATCGAGCGGTCCAGCGGGACAGGTCGTATCT 240
||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||
13780R-2.in silico 181 GTCCTCTGACTTAGAGCCGTACTT<span class="snp"><tt>T</tt></span>GAGGATCGAGCGGTCCAGCGGGACAGGTCGTATCT 240
13780R-2.IR_full 241 CATCCGATTGATCCGCCGGCGCGTGCCACGTGATCAGGCCCCCCAGCAAGTAATTCTTCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13780R-2.in silico 241 CATCCGATTGATCCGCCGGCGCGTGCCACGTGATCAGGCCCCCCAGCAAGTAATTCTTCA 300
13780R-2.IR_full 301 GGGCCGCAGTCTCAGTGGCGTTCACTGGGGGCAGGGCGACGACAATCATCTCAGCTCCGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13780R-2.in silico 301 GGGCCGCAGTCTCAGTGGCGTTCACTGGGGGCAGGGCGACGACAATCATCTCAGCTCCGA 360
13780R-2.IR_full 361 TGCGGCCGTCGATGGGAGCGATGACTATCCATCGGATCAGCCGGGCGATTTGACAGTGTT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13780R-2.in silico 361 TGCGGCCGTCGATGGGAGCGATGACTATCCATCGGATCAGCCGGGCGATTTGACAGTGTT 420
13780R-2.IR_full 421 GAAAGAGCGAATCGC<span class="snp"><tt>A</tt></span>GAACAAAACTCCGTTGAAAAGTTGAGGACTATGAAATACAACAA 480
|||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||
13780R-2.in silico 421 GAAAGAGCGAATCGC<span class="snp"><tt>C</tt></span>GAACAAAACTCCGTTGAAAAGTTGAGGACTATGAAATACAACAA 480
13780R-2.IR_full 481 TAATCGTACCAGGGAGCTCA 500
||||||||||||||||||||
13780R-2.in silico 481 TAATCGTACCAGGGAGCTCA 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_078775.2 |
CG13780-RA (Pvf2), mRNA |
| 0.2 |
1 |
2 |
4 |
2 |
NM_134562.2 |
CG1324-RA (CG1324), mRNA |
| 0 |
0 |
4 |
8 |
6 |
NM_143029.2 |
CG6892-RA, transcript variant A (Ets96B), mRNA |
| 0 |
0 |
4 |
8 |
6 |
NM_206567.1 |
CG6892-RB, transcript variant B (Ets96B), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_141002.1 |
CG11451-RA (CG11451), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_133081.1 |
CG15047-RA (CG15047), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_135019.1 |
CG15627-RA (CG15627), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_140067.1 |
CG3654-RD (CG3654), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_141067.2 |
CG7186-RA (SAK), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_132578.1 |
CG11146-RA (CG11146), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_138206.1 |
CG3371-RA (CG3371), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168383.1 |
CG6718-RB, transcript variant B (CG6718), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168385.1 |
CG6718-RD, transcript variant D (CG6718), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140109.1 |
CG6718-RA, transcript variant A (CG6718), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_168384.1 |
CG6718-RC, transcript variant C (CG6718), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_137510.2 |
CG30122-RB (CG30122), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079456.2 |
CG6625-RA (Snap), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137370.3 |
CG14478-RB, transcript variant B (CG14478), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166229.2 |
CG14478-RA, transcript variant A (CG14478), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079938.3 |
CG2102-RA (cas), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_170550.1 |
CG11337-RB, transcript variant B (CG11337), mRNA |
| 0 |
0 |
1 |
2 |
1 |
NM_057519.3 |
CG17998-RA (Gprk2), mRNA |
| 0 |
0 |
0 |
11 |
11 |
NM_133088.1 |
CG6632-RA (Ing3), mRNA |
| 0 |
0 |
0 |
6 |
11 |
NM_166895.1 |
CG11491-RD, transcript variant D (br), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_131948.1 |
CG3081-RA (CG3081), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_001042868.1 |
CG34124-RA (CG34124), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_079317.2 |
CG10704-RA (toe), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_165812.1 |
CG30029-RA (CG30029), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_135869.1 |
CG3474-RA (CG3474), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_132772.2 |
CG5599-RA (CG5599), mRNA |
|
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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