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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13762R-3

Symbol CG13762 Full Name CG13762

CG No CG13762 Old CG No CG13762

Synonyms EG:BACR7C10.7, CT33244, CG13762

Accession No (Link to NCBI) NM_130657.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCATCGCTGTGTTCGCCGGTTT

Primer Seq. 3'

aatctagaggtaccGCACCAGAGTGGACTCGATGA

Predicted Fragment Size

IR fragment full Seq

0001 CATCGCTGTG TTCGCCGGTT TCCAGCACGA GGACGTCAAG TTCAAGACGA TGCTGGTGAG 
0061 CATTGTGATA GTGCTGATCT TCCAGTTCCT CATCGGTGAC CCGATCAAGT TCGTGATCCT 
0121 GTCCATCGAC CGGGCCCTGT GGCCACCGCG GATCTATATA CCGCCGCGAT GTGACAAGGA 
0181 TGAGATGGAT GAGAAGGAGG AACGCAGGGA CTTCCTCAAG CAGCGTCTGA TTAACAAGCG 
0241 CGCCAACCTG ATGCTGACCT CGCGCTACAG GAACTATAAG CTCAACGATC AGTACAAGAT 
0301 GATCGCCCAC GATCTCTTCA TCTACGGCCC GTACTTTCTC TGCCTGATGT GCCTGGTGCT 
0361 GGTGACGAGG GATCAAACCC TCTACCACAA CACTCGCATC ATCACGGATC TGTTTATGTA 
0421 CAACCACACG GACTACACGG GCCTGCAGGA GGTGTACTTC CTCAACCAGC TGTTCGACTT 
0481 CATCGAGTCC ACTCTGGTGC

in silico PCR Fragment

0001 CATCGCTGTG TTCGCCGGTT TCCAGCACGA GGACGTCAAG TTCAAGACGA TGCTGGTGAG 
0061 CATTGTGATA GTGCTGATCT TCCAGTTCCT CATCGGTGAC CCGATCAAGT TCGTTATCCT 
0121 GTCCATCGAC CGGGCCCTGT GGCCACCGCG GATCTATATA CCGCCGCGAT GTGACAAGGA 
0181 TGAGATGGAT GAGAAGGAGG AACGCAGGGA CTTCCTCAAG CAGCGCCTGA TTAACAAGCG 
0241 CGCCAACCTG ATGCTGACCT CGCGCTACAG GAACTATAAG CTCAACGATC AGTACAAGAT 
0301 GATCGCCCAC GATCTCTTCA TCTACGGCCC GTACTTTCTC TGCCTGATGT GCCTGGTGCT 
0361 GGTGACGAGG GATCAAACCC TCTACCACAA CACTCGCATC ATCACGGATC TGTTTATGTA 
0421 CAACCACACG GACTACACGG GCCTGCAGGA GGTGTACTTC CTCAACCAGC TGTTCGACTT 
0481 CATCGAGTCC ACTCTGGTGC

Assemble Data


13762R-3.IR_full       1   CATCGCTGTGTTCGCCGGTTTCCAGCACGAGGACGTCAAGTTCAAGACGATGCTGGTGAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13762R-3.in silico     1   CATCGCTGTGTTCGCCGGTTTCCAGCACGAGGACGTCAAGTTCAAGACGATGCTGGTGAG 60


13762R-3.IR_full       61  CATTGTGATAGTGCTGATCTTCCAGTTCCTCATCGGTGACCCGATCAAGTTCGTGATCCT 120
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
13762R-3.in silico     61  CATTGTGATAGTGCTGATCTTCCAGTTCCTCATCGGTGACCCGATCAAGTTCGTTATCCT 120


13762R-3.IR_full       121 GTCCATCGACCGGGCCCTGTGGCCACCGCGGATCTATATACCGCCGCGATGTGACAAGGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13762R-3.in silico     121 GTCCATCGACCGGGCCCTGTGGCCACCGCGGATCTATATACCGCCGCGATGTGACAAGGA 180


13762R-3.IR_full       181 TGAGATGGATGAGAAGGAGGAACGCAGGGACTTCCTCAAGCAGCGTCTGATTAACAAGCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||
13762R-3.in silico     181 TGAGATGGATGAGAAGGAGGAACGCAGGGACTTCCTCAAGCAGCGCCTGATTAACAAGCG 240


13762R-3.IR_full       241 CGCCAACCTGATGCTGACCTCGCGCTACAGGAACTATAAGCTCAACGATCAGTACAAGAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13762R-3.in silico     241 CGCCAACCTGATGCTGACCTCGCGCTACAGGAACTATAAGCTCAACGATCAGTACAAGAT 300


13762R-3.IR_full       301 GATCGCCCACGATCTCTTCATCTACGGCCCGTACTTTCTCTGCCTGATGTGCCTGGTGCT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13762R-3.in silico     301 GATCGCCCACGATCTCTTCATCTACGGCCCGTACTTTCTCTGCCTGATGTGCCTGGTGCT 360


13762R-3.IR_full       361 GGTGACGAGGGATCAAACCCTCTACCACAACACTCGCATCATCACGGATCTGTTTATGTA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13762R-3.in silico     361 GGTGACGAGGGATCAAACCCTCTACCACAACACTCGCATCATCACGGATCTGTTTATGTA 420


13762R-3.IR_full       421 CAACCACACGGACTACACGGGCCTGCAGGAGGTGTACTTCCTCAACCAGCTGTTCGACTT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13762R-3.in silico     421 CAACCACACGGACTACACGGGCCTGCAGGAGGTGTACTTCCTCAACCAGCTGTTCGACTT 480


13762R-3.IR_full       481 CATCGAGTCCACTCTGGTGC 500
                           ||||||||||||||||||||
13762R-3.in silico     481 CATCGAGTCCACTCTGGTGC 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_130657.2	CG13762-RA (CG13762), mRNA
0.2	1	4	6	2	NM_132691.2	CG11130-RA (Rtc1), mRNA
0.2	1	2	3	3	NM_139388.1	CG13930-RA (CG13930), mRNA
0	0	4	2	2	NM_058151.4	CG6551-RA (fu), mRNA
0	0	3	3	3	NM_170430.2	CG31037-RA (ca), mRNA
0	0	2	5	7	NM_057466.2	CG8896-RA (18w), mRNA
0	0	2	4	3	NM_079535.2	CG1121-RA (alpha-Est8), mRNA
0	0	2	4	2	NM_135607.2	CG6495-RA (CG6495), mRNA
0	0	2	2	3	NM_057832.3	CG5393-RA, transcript variant A (apt), mRNA
0	0	2	2	3	NM_166609.1	CG5393-RC, transcript variant C (apt), mRNA
0	0	2	2	3	NM_057831.3	CG5393-RB, transcript variant B (apt), mRNA
0	0	2	2	3	NM_166611.1	CG5393-RE, transcript variant E (apt), mRNA
0	0	2	2	3	NM_166610.1	CG5393-RD, transcript variant D (apt), mRNA
0	0	1	4	3	NM_078661.3	CG8649-RA, transcript variant A (Fim), mRNA
0	0	1	4	3	NM_167564.1	CG8649-RC, transcript variant C (Fim), mRNA
0	0	1	4	3	NM_167565.1	CG8649-RD, transcript variant D (Fim), mRNA
0	0	1	3	7	NM_001014606.1	CG1161-RB, transcript variant B (CG1161), mRNA
0	0	1	3	7	NM_141271.2	CG1161-RA, transcript variant A (CG1161), mRNA
0	0	1	3	5	NM_168577.2	CG32134-RA, transcript variant A (btl), mRNA
0	0	1	3	5	NM_001014583.1	CG32134-RB, transcript variant B (btl), mRNA
0	0	1	2	11	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	1	2	8	NM_001043297.1	CG3339-RB, transcript variant B (CG3339), mRNA
0	0	1	2	8	NM_143300.1	CG3339-RA, transcript variant A (CG3339), mRNA
0	0	1	2	6	NM_001032053.1	CG33715-RB, transcript variant B (Msp-300), mRNA
0	0	1	2	5	NM_079574.2	CG9474-RA (Snap24), mRNA
0	0	1	2	4	NM_079580.2	CG6515-RA (Takr86C), mRNA
0	0	1	2	4	NM_164433.1	CG17654-RD, transcript variant D (Eno), mRNA
0	0	1	2	4	NM_164432.1	CG17654-RC, transcript variant C (Eno), mRNA
0	0	1	2	4	NM_164434.1	CG17654-RE, transcript variant E (Eno), mRNA
0	0	1	2	4	NM_164431.1	CG17654-RB, transcript variant B (Eno), mRNA

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