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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13761R-3

Symbol Bzd Full Name Buzidau

CG No CG13761 Old CG No CG13761

Synonyms CG13761, EG:BACR7C10.4, unnamed, Bzd

Accession No (Link to NCBI) NM_080029.3

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCACCGCCACCGTTGGTTCATC

Primer Seq. 3'

aatctagaggtaccTGAGGACGGCATGCAGCGAGT

Predicted Fragment Size

IR fragment full Seq

0001 CACCGCCACC GTTGGTTCAT CCAGATCCGC AACCACATCC GCAACCGCAT CCGCATGCTC 
0061 ATCCAAATCC AAGAACCTAA AGAATCCGGC GCCGCAAATA AAAAGGGGAC AGCGGATCCT 
0121 CACGGAGAAA CCATTCGCCT TCGTCTTGAA ATCACAGTAC CGCCTGGAGC GGTGCGAATA 
0181 ACTGCCTGGA GGCGACCAAG GTGCTGAAGT GCTCCAACTG CAGGTATGTG TCCTACTGCC 
0241 ACCGCTCATG CCAGATGCAA GCTTGGGGCC AGCACAAGCA CGAGTGTCCT TTCCTCAAGA 
0301 AGGTGCATCC TCGAGTTGTG CCCGATGCTG CCCGAATGCT CTGCCGACTG ATCCTGCGCC 
0361 TGGAGCACGG CGGNAGACCT GATCCGCGGC TATTACACCG AGCACGGCTC CCGCAAGTTT 
0421 CGCGATCTCA TGTCCCATTA CGCCGAAATC AAGAACGATC CCATGCGACT GGAGCACTTG 
0481 GACTCGCTGC ATGCCGTCCT CA

in silico PCR Fragment

0001 CACCGCCACC GTTGGTTCAT CCAGATCCGC AACCACATCC GCAACCGCAT CCGCATGCTC 
0061 ATCCAAATCC AAGAACCTAA AGAATCCGGC GCCGCAAATA AAAAGGGGAC AGCGGATCCT 
0121 CACGGAGAAA CCATTCGCCT TCGTCTTGAA ATCACAGTAC CGCCTGGAGC GGTGCG-ATA 
0181 ACTGCCTGGA GGCGACCAAG GTGCTGAAGT GCTCCAACTG CAGGTATGTG TCCTACTGCC 
0241 ACCGCTCATG CCAGATGCAA GCTTGGGGCC AGCACAAGCA CGAGTGTCCT TTCCTCAAGA 
0301 AGGTGCATCC TCGAGTTGTG CCCGATGCTG CCCGAATGCT CTGCCGACTG ATCCTGCGCC 
0361 TGGAGCACGG CGG-AGACCT GATCCGCGGC TATTACACCG AGCACGGCTC CCGCAAGTTT 
0421 CGCGATCTCA TGTCCCATTA CGCCGAAATC AAGAACGATC CCATGCGACT GGAGCACTTG 
0481 GACTCGCTGC ATGCCGTCCT CA

Assemble Data


13761R-3.IR_full       1   CACCGCCACCGTTGGTTCATCCAGATCCGCAACCACATCCGCAACCGCATCCGCATGCTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13761R-3.in silico     1   CACCGCCACCGTTGGTTCATCCAGATCCGCAACCACATCCGCAACCGCATCCGCATGCTC 60


13761R-3.IR_full       61  ATCCAAATCCAAGAACCTAAAGAATCCGGCGCCGCAAATAAAAAGGGGACAGCGGATCCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13761R-3.in silico     61  ATCCAAATCCAAGAACCTAAAGAATCCGGCGCCGCAAATAAAAAGGGGACAGCGGATCCT 120


13761R-3.IR_full       121 CACGGAGAAACCATTCGCCTTCGTCTTGAAATCACAGTACCGCCTGGAGCGGTGCGAATA 180
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
13761R-3.in silico     121 CACGGAGAAACCATTCGCCTTCGTCTTGAAATCACAGTACCGCCTGGAGCGGTGCG-ATA 180


13761R-3.IR_full       181 ACTGCCTGGAGGCGACCAAGGTGCTGAAGTGCTCCAACTGCAGGTATGTGTCCTACTGCC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13761R-3.in silico     181 ACTGCCTGGAGGCGACCAAGGTGCTGAAGTGCTCCAACTGCAGGTATGTGTCCTACTGCC 240


13761R-3.IR_full       241 ACCGCTCATGCCAGATGCAAGCTTGGGGCCAGCACAAGCACGAGTGTCCTTTCCTCAAGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13761R-3.in silico     241 ACCGCTCATGCCAGATGCAAGCTTGGGGCCAGCACAAGCACGAGTGTCCTTTCCTCAAGA 300


13761R-3.IR_full       301 AGGTGCATCCTCGAGTTGTGCCCGATGCTGCCCGAATGCTCTGCCGACTGATCCTGCGCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13761R-3.in silico     301 AGGTGCATCCTCGAGTTGTGCCCGATGCTGCCCGAATGCTCTGCCGACTGATCCTGCGCC 360


13761R-3.IR_full       361 TGGAGCACGGCGGNAGACCTGATCCGCGGCTATTACACCGAGCACGGCTCCCGCAAGTTT 420
                           ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
13761R-3.in silico     361 TGGAGCACGGCGG-AGACCTGATCCGCGGCTATTACACCGAGCACGGCTCCCGCAAGTTT 420


13761R-3.IR_full       421 CGCGATCTCATGTCCCATTACGCCGAAATCAAGAACGATCCCATGCGACTGGAGCACTTG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13761R-3.in silico     421 CGCGATCTCATGTCCCATTACGCCGAAATCAAGAACGATCCCATGCGACTGGAGCACTTG 480


13761R-3.IR_full       481 GACTCGCTGCATGCCGTCCTCA 502
                           ||||||||||||||||||||||
13761R-3.in silico     481 GACTCGCTGCATGCCGTCCTCA 502

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	2	10	10	NM_080029.3	CG13761-RB (Bzd), mRNA
0.62	3	9	24	61	NM_167487.1	CG32577-RA (disco-r), mRNA
0	0	4	9	25	NM_080531.2	CG5481-RA (lea), mRNA
0	0	4	5	13	NM_169560.1	CG2988-RA (ems), mRNA
0	0	3	13	23	NM_167647.2	CG12199-RB, transcript variant B (kek5), mRNA
0	0	3	13	23	NM_133154.2	CG12199-RA, transcript variant A (kek5), mRNA
0	0	3	6	9	NM_078651.2	CG18582-RA (mbt), mRNA
0	0	3	3	15	NM_167455.1	CG12708-RA (CG12708), mRNA
0	0	3	3	14	NM_079491.2	CG5723-RB (Ten-m), mRNA
0	0	2	10	28	NM_001038902.1	CG33993-RA (CG33993), mRNA
0	0	2	8	6	NM_137177.2	CG11808-RA (CG11808), mRNA
0	0	2	7	16	NM_136732.1	CG12904-RA (CG12904), mRNA
0	0	2	2	5	NM_136267.3	CG3305-RA (CG3305), mRNA
0	0	2	2	3	NM_136367.1	CG30431-RA (CG30431), mRNA
0	0	2	2	3	NM_134917.2	CG15406-RA (CG15406), mRNA
0	0	2	2	2	NM_078943.1	CG12931-RA (Or45b), mRNA
0	0	1	14	15	NM_206567.1	CG6892-RB, transcript variant B (Ets96B), mRNA
0	0	1	14	15	NM_143029.2	CG6892-RA, transcript variant A (Ets96B), mRNA
0	0	1	12	21	NM_176603.1	CG31000-RH, transcript variant H (heph), mRNA
0	0	1	12	14	NM_139364.2	CG1049-RA, transcript variant A (Cct1), mRNA
0	0	1	12	14	NM_167893.1	CG1049-RC, transcript variant C (Cct1), mRNA
0	0	1	12	14	NM_167892.1	CG1049-RB, transcript variant B (Cct1), mRNA
0	0	1	12	14	NM_167894.1	CG1049-RD, transcript variant D (Cct1), mRNA
0	0	1	10	37	NM_167441.2	CG9176-RB, transcript variant B (cngl), mRNA
0	0	1	10	36	NM_078608.3	CG9176-RC, transcript variant C (cngl), mRNA
0	0	1	10	18	NM_078772.2	CG13772-RA (neuroligin), mRNA
0	0	1	9	34	NM_137146.1	CG12856-RA (CG12856), mRNA
0	0	1	9	34	NM_080074.2	CG10197-RA (kn), mRNA
0	0	1	9	24	NM_132787.1	CG15032-RA (CG15032), mRNA
0	0	1	9	13	NM_133114.2	CG32541-RA (CG32541), mRNA

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