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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13733R-1 
 Symbol CG13733  Full Name CG13733 
 CG No CG13733  Old CG No CG13733 
 Synonyms CG13733 
 Accession No (Link to NCBI) NM_140729.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTCGAGCCAAAGCCCGATTGGA 
 Primer Seq. 3' 
aatctagaggtaccGGGCTTCGTACCTGGAGATGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TCGAGCCAAA GCCCGATTGG AGAACGAGGT GGTTCTGCTG CGCGAgCGAG GTGTcNTCGC 
0061 TGCGCTCCAA GCACAACCAA CATCGTAAGT ATCGGCAGGA GCAGGCGGTT NCTGCAGGCG 
0121 CGTATGGAAC AGGAGATCCA GCTTCTACAG AGCGAACTAG CCGCAGCTCA ATCGAATCGG 
0181 AATGAGCGGG AGTGCGGTGG AGCCGAGCAG TGTTCAGTGT TGAGCCAGCA GCTGGAGAAA 
0241 CTTGACGAGC ACGTGGCTAA GCAGGAGAAG TACATTGCCT TCCTGGAGGA GCAGATCAAT 
0301 CTCACACGTA ACAAGTACCA GCAACGTATG ACCGAAGTCC GGCAGAATGC CGAACTGGTG 
0361 GAAAAGGAGA TGAAGCGGGT GCGCCGCGAG ATGAAGACCA TTGCCGAGCA GGCTGGGGAA 
0421 GTGGACAGCC TCAAGAACCA AGTCGGTTTC CTCACCGCCA AGCTGGAGCG CCGGAACGCC 
0481 ATCATCTCCA GGTACGAAGC CC 
 in silico PCR Fragment 
0001 TCGAGCCAAA GCCCGATTGG AGAACGAGGT GGTTCTCCTG CG-GAGCGAG GTGTCCTCGC 
0061 TGCGCTCCAA GCACAACCAA CATCGTAAGT ATCGGCAGGA GCAGGTGGTT -CTGCAGGCG 
0121 CGTATGGAAC AGGAGATCCA GCTTCTACAG AGCGAACTAG CCGCAGCTCA ATCGAATCGG 
0181 AATGAGCGGG AGTGCGGTGG AGCCGAGCAG TGTTCAGTGT TGAGCCAGCA GCTGGAGAAA 
0241 CTTGACGAGC ACGTGGCTAA GCAGGAGAAG TACATTGCCT TCCTGGAGGA GCAGATCAAT 
0301 CTCACACGTA ACAAGTACCA GCAACGTATG ACCGAAGTCC GGCAGAATGC CGAACTGGTG 
0361 GAAAAGGAGA TGAAGCGGGT GCGCCGCGAG ATGAAGACCA TTGCCGAGCA GGCTGGGGAA 
0421 GTGGACAGCC TCAAGAACCA AGTCGGTTTC CTCACCGCCA AGCTGGAGCG CCGGAACGCC 
0481 ATCATCTCCA GGTACGAAGC CC 
 Assemble Data 
<PRE>

13733R-1.IR_full       1   TCGAGCCAAAGCCCGATTGGAGAACGAGGTGGTTCT<span class="snp"><tt>G</tt></span>CTGCG<span class="snp"><tt>C</tt></span>GAGCGAGGTGTC<span class="snp"><tt>N</tt></span>TCGC 60
                           ||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||
13733R-1.in silico     1   TCGAGCCAAAGCCCGATTGGAGAACGAGGTGGTTCT<span class="snp"><tt>C</tt></span>CTGCG<span class="snp"><tt>-</tt></span>GAGCGAGGTGTC<span class="snp"><tt>C</tt></span>TCGC 60


13733R-1.IR_full       61  TGCGCTCCAAGCACAACCAACATCGTAAGTATCGGCAGGAGCAGG<span class="snp"><tt>C</tt></span>GGTT<span class="snp"><tt>N</tt></span>CTGCAGGCG 120
                           |||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||
13733R-1.in silico     61  TGCGCTCCAAGCACAACCAACATCGTAAGTATCGGCAGGAGCAGG<span class="snp"><tt>T</tt></span>GGTT<span class="snp"><tt>-</tt></span>CTGCAGGCG 120


13733R-1.IR_full       121 CGTATGGAACAGGAGATCCAGCTTCTACAGAGCGAACTAGCCGCAGCTCAATCGAATCGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13733R-1.in silico     121 CGTATGGAACAGGAGATCCAGCTTCTACAGAGCGAACTAGCCGCAGCTCAATCGAATCGG 180


13733R-1.IR_full       181 AATGAGCGGGAGTGCGGTGGAGCCGAGCAGTGTTCAGTGTTGAGCCAGCAGCTGGAGAAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13733R-1.in silico     181 AATGAGCGGGAGTGCGGTGGAGCCGAGCAGTGTTCAGTGTTGAGCCAGCAGCTGGAGAAA 240


13733R-1.IR_full       241 CTTGACGAGCACGTGGCTAAGCAGGAGAAGTACATTGCCTTCCTGGAGGAGCAGATCAAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13733R-1.in silico     241 CTTGACGAGCACGTGGCTAAGCAGGAGAAGTACATTGCCTTCCTGGAGGAGCAGATCAAT 300


13733R-1.IR_full       301 CTCACACGTAACAAGTACCAGCAACGTATGACCGAAGTCCGGCAGAATGCCGAACTGGTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13733R-1.in silico     301 CTCACACGTAACAAGTACCAGCAACGTATGACCGAAGTCCGGCAGAATGCCGAACTGGTG 360


13733R-1.IR_full       361 GAAAAGGAGATGAAGCGGGTGCGCCGCGAGATGAAGACCATTGCCGAGCAGGCTGGGGAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13733R-1.in silico     361 GAAAAGGAGATGAAGCGGGTGCGCCGCGAGATGAAGACCATTGCCGAGCAGGCTGGGGAA 420


13733R-1.IR_full       421 GTGGACAGCCTCAAGAACCAAGTCGGTTTCCTCACCGCCAAGCTGGAGCGCCGGAACGCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13733R-1.in silico     421 GTGGACAGCCTCAAGAACCAAGTCGGTTTCCTCACCGCCAAGCTGGAGCGCCGGAACGCC 480


13733R-1.IR_full       481 ATCATCTCCAGGTACGAAGCCC 502
                           ||||||||||||||||||||||
13733R-1.in silico     481 ATCATCTCCAGGTACGAAGCCC 502

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140729.1  CG13733-RA (CG13733), mRNA 
0   0  4  4  8  NM_142742.2  CG6332-RA (CG6332), mRNA 
0   0  4  4  4  NM_078760.2  CG6992-RI (GluRIIA), mRNA 
0   0  4  4  2  NM_140323.1  CG10681-RA (CG10681), mRNA 
0   0  2  6  5  NM_135766.2  CG16974-RA (CG16974), mRNA 
0   0  2  2  6  NM_078717.2  CG3696-RA, transcript variant A (kis), mRNA 
0   0  1  7  4  NM_137031.2  CG4840-RA (CG4840), mRNA 
0   0  1  6  4  NM_132308.1  CG12106-RA (CG12106), mRNA 
0   0  1  4  12  NM_133075.2  CG6461-RA (CG6461), mRNA 
0   0  1  4  3  NM_001015256.1  CG17167-PB (CG17167), mRNA 
0   0  1  4  3  NM_001015257.1  CG17167-PA (CG17167), mRNA 
0   0  1  3  4  NM_057953.3  CG3998-RA (zf30C), mRNA 
0   0  1  2  6  NM_134551.4  CG32506-RA (CG32506), mRNA 
0   0  1  2  6  NM_057719.3  CG8274-RA (Mtor), mRNA 
0   0  1  2  5  NM_168113.2  CG32423-RD, transcript variant D (alan-shepard), mRNA 
0   0  1  2  4  NM_206153.1  CG33130-RC, transcript variant C (l(2)k07433), mRNA 
0   0  1  2  4  NM_136741.2  CG11763-RC, transcript variant C (micr), mRNA 
0   0  1  2  4  NM_165770.1  CG11763-RA, transcript variant A (micr), mRNA 
0   0  1  2  4  NM_001038849.1  CG11763-RD, transcript variant D (micr), mRNA 
0   0  1  2  2  NM_137385.1  CG18432-RA (CG18432), mRNA 
0   0  1  2  2  NM_166580.1  CG32834-RA (CG32834), mRNA 
0   0  0  8  3  NM_136391.2  CG9436-RA (CG9436), mRNA 
0   0  0  7  7  NM_135156.2  CG13991-RA (CG13991), mRNA 
0   0  0  5  11  NM_141289.2  CG2519-RA, transcript variant A (CG2519), mRNA 
0   0  0  5  11  NM_206431.1  CG2519-RB, transcript variant B (CG2519), mRNA 
0   0  0  5  8  NM_057245.2  CG6189-RA (l(1)1Bi), mRNA 
0   0  0  5  4  NM_132456.1  CG11126-RA (CG11126), mRNA 
0   0  0  5  3  NM_165027.1  CG31849-RA (CG31849), mRNA 
0   0  0  5  2  NM_140491.2  CG13458-RA (CG13458), mRNA 
0   0  0  4  11  NM_001043297.1  CG3339-RB, transcript variant B (CG3339), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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