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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13681R-3

Symbol CG13681 Full Name CG13681

CG No CG13681 Old CG No CG13681

Synonyms CG13681

Accession No (Link to NCBI) NM_139913.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGAACTCACCACAGCTGGCG

Primer Seq. 3'

aatctagaggtaccATCACAGGCGACATGTCCTTG

Predicted Fragment Size

IR fragment full Seq

0001 ATGAACTCAC CACAGCTGGC GCCCACCACT GCGCAATCTC ACCACGCTGA GAGCGCTGAC 
0061 AAAACAGCGC CGCCTGGAAA TAAATATGCG CCTGGAAGAA GGCTAGCTGC GCGGCAAACT 
0121 CTTCGATTGG CAATACCGAA GGCGCAACCC GGTGAACCAC TGAATCACCC AACGAGTGTC 
0181 GGCCTCTTTC GTCCCTCACC GTCGCAACTG CCACCGGCAC CGATTTTGAA GCGCGTTCCA 
0241 GCGGTCAGCG GCGGGTGCAG TGGAATACCG AAGTCCGCCT CGCCACTGGA ATCGCACCAC 
0301 CTGCCCAATG TCACGCCCAC CAACGCGCAC ACCCGTTTGG GCTATGGCAT GCAGGGCGCG 
0361 GGGGGTGGCA GTGGCTGTTC CAGTGCCTCA TCAGCATCCA ATTTTCTGGG CGTGCGCAAG 
0421 CCCAGCCTGA CCTTGAACTC ATCGGATATC CGGAACAACA ACGATCTGTT CGCCTTCCCC 
0481 AAGGACATGT CGCCTGTGAT

in silico PCR Fragment

0001 ATGAACTCAC CACAGCTGGC GCCCACCACT GCGCAATCTC ACCACGCTGA GAGCGCTGAC 
0061 AAAACAGCGC CGCCTGGAAA TAAATATGCG CCTGGAAGAA GGCTAGCTGC GCGGCAAACT 
0121 CTTCGATTGG CAATACCGAA GGCGCAACCC GGTGACCCAC TGAATCACCC AACGAGTGTC 
0181 GGCCTCTTTC GTCCCTCACC ATCGCAACTG CCACCGGCAC CGATTTTGAA GCGCGTTCCA 
0241 GCGGTCAGCG GCGGGTGCAG TGGAATACCG AAGTCCGCCT CGCCACTGGA ATCGCACCAC 
0301 CTGCCCAATG TCACGCCCAC CAACGCGCAC ACCCGTTTGG GCTATGGCAT GCAGGGCGCG 
0361 GGGGGAGGCA GTAGCTGTTC CAGTGCCTCA TCAGCATCCA ATTTTCTGGG CGTGCGCAAG 
0421 CCCAGCCTGA CCTTGAACTC ATCGGATATC CGGAACAACA ACGATCTGTT CGCCTTCCCC 
0481 AAGGACATGT CGCCTGTGAT

Assemble Data


13681R-3.IR_full       1   ATGAACTCACCACAGCTGGCGCCCACCACTGCGCAATCTCACCACGCTGAGAGCGCTGAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-3.in silico     1   ATGAACTCACCACAGCTGGCGCCCACCACTGCGCAATCTCACCACGCTGAGAGCGCTGAC 60


13681R-3.IR_full       61  AAAACAGCGCCGCCTGGAAATAAATATGCGCCTGGAAGAAGGCTAGCTGCGCGGCAAACT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-3.in silico     61  AAAACAGCGCCGCCTGGAAATAAATATGCGCCTGGAAGAAGGCTAGCTGCGCGGCAAACT 120


13681R-3.IR_full       121 CTTCGATTGGCAATACCGAAGGCGCAACCCGGTGAACCACTGAATCACCCAACGAGTGTC 180
                           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
13681R-3.in silico     121 CTTCGATTGGCAATACCGAAGGCGCAACCCGGTGACCCACTGAATCACCCAACGAGTGTC 180


13681R-3.IR_full       181 GGCCTCTTTCGTCCCTCACCGTCGCAACTGCCACCGGCACCGATTTTGAAGCGCGTTCCA 240
                           |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
13681R-3.in silico     181 GGCCTCTTTCGTCCCTCACCATCGCAACTGCCACCGGCACCGATTTTGAAGCGCGTTCCA 240


13681R-3.IR_full       241 GCGGTCAGCGGCGGGTGCAGTGGAATACCGAAGTCCGCCTCGCCACTGGAATCGCACCAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-3.in silico     241 GCGGTCAGCGGCGGGTGCAGTGGAATACCGAAGTCCGCCTCGCCACTGGAATCGCACCAC 300


13681R-3.IR_full       301 CTGCCCAATGTCACGCCCACCAACGCGCACACCCGTTTGGGCTATGGCATGCAGGGCGCG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-3.in silico     301 CTGCCCAATGTCACGCCCACCAACGCGCACACCCGTTTGGGCTATGGCATGCAGGGCGCG 360


13681R-3.IR_full       361 GGGGGTGGCAGTGGCTGTTCCAGTGCCTCATCAGCATCCAATTTTCTGGGCGTGCGCAAG 420
                           ||||| |||||| |||||||||||||||||||||||||||||||||||||||||||||||
13681R-3.in silico     361 GGGGGAGGCAGTAGCTGTTCCAGTGCCTCATCAGCATCCAATTTTCTGGGCGTGCGCAAG 420


13681R-3.IR_full       421 CCCAGCCTGACCTTGAACTCATCGGATATCCGGAACAACAACGATCTGTTCGCCTTCCCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-3.in silico     421 CCCAGCCTGACCTTGAACTCATCGGATATCCGGAACAACAACGATCTGTTCGCCTTCCCC 480


13681R-3.IR_full       481 AAGGACATGTCGCCTGTGAT 500
                           ||||||||||||||||||||
13681R-3.in silico     481 AAGGACATGTCGCCTGTGAT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	2	2	NM_139913.1	CG13681-RA (CG13681), mRNA
0.2	1	2	2	5	NM_136091.3	CG10473-RA, transcript variant A (CG10473), mRNA
0.2	1	2	2	3	NM_165273.1	CG10473-RB, transcript variant B (CG10473), mRNA
0	0	4	3	4	NM_167474.1	CG8909-RB (CG8909), mRNA
0	0	4	2	4	NM_169882.1	CG4836-RB, transcript variant B (CG4836), mRNA
0	0	4	2	4	NM_169883.1	CG4836-RA, transcript variant A (CG4836), mRNA
0	0	4	2	4	NM_142599.1	CG4836-RC, transcript variant C (CG4836), mRNA
0	0	3	3	13	NM_057941.3	CG5227-RA, transcript variant A (sdk), mRNA
0	0	3	3	13	NM_134314.2	CG5227-RB, transcript variant B (sdk), mRNA
0	0	3	3	13	NM_134315.2	CG5227-RD, transcript variant D (sdk), mRNA
0	0	3	3	13	NM_057942.3	CG5227-RC, transcript variant C (sdk), mRNA
0	0	2	4	2	NM_057882.3	CG1609-RA (Gcn2), mRNA
0	0	2	3	3	NM_137546.2	CG15105-RA, transcript variant A (CG15105), mRNA
0	0	2	3	3	NM_166318.1	CG15105-RB, transcript variant B (CG15105), mRNA
0	0	2	3	2	NM_131951.1	CG15473-RA (CG15473), mRNA
0	0	2	2	4	NM_137806.2	CG5465-RA (MED16), mRNA
0	0	1	4	2	NM_078871.4	CG10305-RB, transcript variant B (RpS26), mRNA
0	0	1	4	2	NM_165252.1	CG10305-RA, transcript variant A (RpS26), mRNA
0	0	1	4	2	NM_165253.1	CG10305-RC, transcript variant C (RpS26), mRNA
0	0	1	3	5	NM_143704.1	CG2916-RB, transcript variant B (Sep5), mRNA
0	0	1	3	5	NM_165578.1	CG2916-RA, transcript variant A (Sep5), mRNA
0	0	1	3	4	NM_168571.2	CG32133-RA (CG32133), mRNA
0	0	1	3	4	NM_142367.1	CG5866-RA (CG5866), mRNA
0	0	1	3	2	NM_079159.2	CG1004-RA (rho), mRNA
0	0	1	3	2	NM_132068.1	CG15899-RB (Ca-alpha1T), mRNA
0	0	1	2	4	NM_001038730.1	CG3711-RB, transcript variant B (CG3711), mRNA
0	0	1	2	4	NM_130513.2	CG3711-RA, transcript variant A (CG3711), mRNA
0	0	1	2	2	NM_141998.1	CG11670-RA (CG11670), mRNA
0	0	0	7	3	NM_165497.1	CG3358-RA, transcript variant A (CG3358), mRNA
0	0	0	7	3	NM_136404.2	CG3358-RB, transcript variant B (CG3358), mRNA

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