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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13681R-1 |
| Symbol |
CG13681 |
Full Name |
CG13681 |
| CG No |
CG13681 |
Old CG No |
CG13681 |
| Synonyms |
CG13681 |
| Accession No (Link to NCBI) |
NM_139913.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52
[
PubMed ID = 22798488
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGAACTCACCACAGCTGGCG |
| Primer Seq. 3' |
aatctagaggtaccATCACAGGCGACATGTCCTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGAACTCAC CACAGCTGGC GCCCACCACT GCGCAATCTC ACCACGCTGA GAGCGCTGAC
0061 AAAACAGCGC CGCCTGGAAA TAAATATGCG CCTGGAAGAA GGCTAGCTGC GCGGCAAACT
0121 CTTCGATTGG CAATACCGAA GGCGCAACCC GGTGAACCAC TGAATCACCC AACGAGTGTC
0181 GGCCTCTTTC GTCCCTCACC GTCGCAACTG CCACCGGCAC CGATTTTGAA GCGCGTTCCA
0241 GCGGTCAGCG GCGGGTGCAG TGGAATACCG AAGTCCGCCT CGCCACTGGA ATCGCACCAC
0301 CTGCCCAATG TCACGCCCAC CAACGCGCAC ACCCGTTTGG GCTATGGCAT GCAGGGCGCG
0361 GGGGGTGGCA GTGGCTGTTC CAGTGCCTCA TCAGCATCCA ATTTTCTGGG CGTGCGCAAG
0421 CCCAGCCTGA CCTTGAACTC ATCGGATATC CGGAACAACA ACGATCTGTT CGCCTTCCCC
0481 AAGGACATGT CGCCTGTGAT |
| in silico PCR Fragment |
0001 ATGAACTCAC CACAGCTGGC GCCCACCACT GCGCAATCTC ACCACGCTGA GAGCGCTGAC
0061 AAAACAGCGC CGCCTGGAAA TAAATATGCG CCTGGAAGAA GGCTAGCTGC GCGGCAAACT
0121 CTTCGATTGG CAATACCGAA GGCGCAACCC GGTGACCCAC TGAATCACCC AACGAGTGTC
0181 GGCCTCTTTC GTCCCTCACC ATCGCAACTG CCACCGGCAC CGATTTTGAA GCGCGTTCCA
0241 GCGGTCAGCG GCGGGTGCAG TGGAATACCG AAGTCCGCCT CGCCACTGGA ATCGCACCAC
0301 CTGCCCAATG TCACGCCCAC CAACGCGCAC ACCCGTTTGG GCTATGGCAT GCAGGGCGCG
0361 GGGGGAGGCA GTAGCTGTTC CAGTGCCTCA TCAGCATCCA ATTTTCTGGG CGTGCGCAAG
0421 CCCAGCCTGA CCTTGAACTC ATCGGATATC CGGAACAACA ACGATCTGTT CGCCTTCCCC
0481 AAGGACATGT CGCCTGTGAT |
| Assemble Data |
13681R-1.IR_full 1 ATGAACTCACCACAGCTGGCGCCCACCACTGCGCAATCTCACCACGCTGAGAGCGCTGAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-1.in silico 1 ATGAACTCACCACAGCTGGCGCCCACCACTGCGCAATCTCACCACGCTGAGAGCGCTGAC 60
13681R-1.IR_full 61 AAAACAGCGCCGCCTGGAAATAAATATGCGCCTGGAAGAAGGCTAGCTGCGCGGCAAACT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-1.in silico 61 AAAACAGCGCCGCCTGGAAATAAATATGCGCCTGGAAGAAGGCTAGCTGCGCGGCAAACT 120
13681R-1.IR_full 121 CTTCGATTGGCAATACCGAAGGCGCAACCCGGTGAACCACTGAATCACCCAACGAGTGTC 180
||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
13681R-1.in silico 121 CTTCGATTGGCAATACCGAAGGCGCAACCCGGTGACCCACTGAATCACCCAACGAGTGTC 180
13681R-1.IR_full 181 GGCCTCTTTCGTCCCTCACCGTCGCAACTGCCACCGGCACCGATTTTGAAGCGCGTTCCA 240
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
13681R-1.in silico 181 GGCCTCTTTCGTCCCTCACCATCGCAACTGCCACCGGCACCGATTTTGAAGCGCGTTCCA 240
13681R-1.IR_full 241 GCGGTCAGCGGCGGGTGCAGTGGAATACCGAAGTCCGCCTCGCCACTGGAATCGCACCAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-1.in silico 241 GCGGTCAGCGGCGGGTGCAGTGGAATACCGAAGTCCGCCTCGCCACTGGAATCGCACCAC 300
13681R-1.IR_full 301 CTGCCCAATGTCACGCCCACCAACGCGCACACCCGTTTGGGCTATGGCATGCAGGGCGCG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-1.in silico 301 CTGCCCAATGTCACGCCCACCAACGCGCACACCCGTTTGGGCTATGGCATGCAGGGCGCG 360
13681R-1.IR_full 361 GGGGGTGGCAGTGGCTGTTCCAGTGCCTCATCAGCATCCAATTTTCTGGGCGTGCGCAAG 420
||||| |||||| |||||||||||||||||||||||||||||||||||||||||||||||
13681R-1.in silico 361 GGGGGAGGCAGTAGCTGTTCCAGTGCCTCATCAGCATCCAATTTTCTGGGCGTGCGCAAG 420
13681R-1.IR_full 421 CCCAGCCTGACCTTGAACTCATCGGATATCCGGAACAACAACGATCTGTTCGCCTTCCCC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13681R-1.in silico 421 CCCAGCCTGACCTTGAACTCATCGGATATCCGGAACAACAACGATCTGTTCGCCTTCCCC 480
13681R-1.IR_full 481 AAGGACATGTCGCCTGTGAT 500
||||||||||||||||||||
13681R-1.in silico 481 AAGGACATGTCGCCTGTGAT 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
2 |
2 |
NM_139913.1 |
CG13681-RA (CG13681), mRNA |
| 0.2 |
1 |
2 |
2 |
5 |
NM_136091.3 |
CG10473-RA, transcript variant A (CG10473), mRNA |
| 0.2 |
1 |
2 |
2 |
3 |
NM_165273.1 |
CG10473-RB, transcript variant B (CG10473), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_167474.1 |
CG8909-RB (CG8909), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_169882.1 |
CG4836-RB, transcript variant B (CG4836), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_169883.1 |
CG4836-RA, transcript variant A (CG4836), mRNA |
| 0 |
0 |
4 |
2 |
4 |
NM_142599.1 |
CG4836-RC, transcript variant C (CG4836), mRNA |
| 0 |
0 |
3 |
3 |
13 |
NM_057941.3 |
CG5227-RA, transcript variant A (sdk), mRNA |
| 0 |
0 |
3 |
3 |
13 |
NM_134314.2 |
CG5227-RB, transcript variant B (sdk), mRNA |
| 0 |
0 |
3 |
3 |
13 |
NM_134315.2 |
CG5227-RD, transcript variant D (sdk), mRNA |
| 0 |
0 |
3 |
3 |
13 |
NM_057942.3 |
CG5227-RC, transcript variant C (sdk), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_057882.3 |
CG1609-RA (Gcn2), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_137546.2 |
CG15105-RA, transcript variant A (CG15105), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_166318.1 |
CG15105-RB, transcript variant B (CG15105), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_131951.1 |
CG15473-RA (CG15473), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_137806.2 |
CG5465-RA (MED16), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_078871.4 |
CG10305-RB, transcript variant B (RpS26), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_165252.1 |
CG10305-RA, transcript variant A (RpS26), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_165253.1 |
CG10305-RC, transcript variant C (RpS26), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_143704.1 |
CG2916-RB, transcript variant B (Sep5), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_165578.1 |
CG2916-RA, transcript variant A (Sep5), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_168571.2 |
CG32133-RA (CG32133), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_142367.1 |
CG5866-RA (CG5866), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_079159.2 |
CG1004-RA (rho), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132068.1 |
CG15899-RB (Ca-alpha1T), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001038730.1 |
CG3711-RB, transcript variant B (CG3711), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_130513.2 |
CG3711-RA, transcript variant A (CG3711), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_141998.1 |
CG11670-RA (CG11670), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_165497.1 |
CG3358-RA, transcript variant A (CG3358), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_136404.2 |
CG3358-RB, transcript variant B (CG3358), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| + |
|
| VM |
 |
| +- |
|
| VB |
 |
| +- |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
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