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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

13674R-7

Symbol

CG13674

Full Name

CG13674

CG No

CG13674

Old CG No

CG13674

Synonyms

CG13674

Accession No (Link to NCBI)

NM_139935.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

semi-lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTCTTCGCTGTTGTGGCTGTG

Primer Seq. 3'

aatctagaggtaccTTCTTCAGCAGAACTGGGGCA

Predicted Fragment Size

IR fragment full Seq

0001 TTCTTCGCTG TTGTGGCTGT GNCTGCTGCC AAACCCGGTA TTGTGGCTCC TCTGGCCTAC 
0061 ACCGCTCCGG CTGTGGTGGG CAGTGCCGCC TACGTGGCTC CCTTACGCCT CCAAGCTACA 
0121 CCGCCAACTC GGTGGCCCAC AGCGCCGCCT NTCCCAGCTG CCTACACCGC CGCCTACACC 
0181 GCTCCCGTTG CTGCAGCCNT ACGCCGCTCC AGTGGCTGCT GCTTATACCG CTCCAGTGGC 
0241 CGCTGCGTAC GNCGCCCCAG CTGNCTATAC CGCTGCCTAC ACCGCCCCCA TTGCCCGTTA 
0301 TGCCGCCACC CCCTTCGCAG CACCCATCGC CGCTCCCGTG GCTGCCGCCT ACACCGCCCC 
0361 CANTCGCCGC NGCTGCCCCA GTTCTGCTGA AGAA

in silico PCR Fragment

0001 TTCTTCGCTG TTGTGGCTGT GGCTGCTGCC AAACCCGGTA TTGTGGCTCC TCTGGCCTAC 
0061 ACCGCTCCGG CTGTGGTGGG CAGTGCCGCC TACGTGGCTC CC-TACGCCT CC-AGCTACA 
0121 CCGCCAACTC GGTGGCCCAC AGCGCCGCCT -TCCCAGCTG CCTACACCGC CGCCTACACT 
0181 GCTCCCGTTG CTGCTGCC-T ATACCGCTCC AGTGGCTGCT GCTTATACCG CTCCAGTGGC 
0241 CGCTGCGTAC GCCGCCCCAG CTGCCTATAC CGCTGCCTAC ACCGCCCCCA TTGCCCGTTA 
0301 TGCCGCCACC CCCTTCGCAG CACCCATCGC CGCTCCCGTG GCTGCCGCCT ACACCGCCCC 
0361 CA-TCGCCGC CGCTGCCCCA GTTCTGCTGA AGAA

Assemble Data

<PRE>

13674R-7.IR_full       1   TTCTTCGCTGTTGTGGCTGTG<span class="snp"><tt>N</tt></span>CTGCTGCCAAACCCGGTATTGTGGCTCCTCTGGCCTAC 60
                           |||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||
13674R-7.in silico     1   TTCTTCGCTGTTGTGGCTGTG<span class="snp"><tt>G</tt></span>CTGCTGCCAAACCCGGTATTGTGGCTCCTCTGGCCTAC 60


13674R-7.IR_full       61  ACCGCTCCGGCTGTGGTGGGCAGTGCCGCCTACGTGGCTCCC<span class="snp"><tt>T</tt></span>TACGCCTCC<span class="snp"><tt>A</tt></span>AGCTACA 120
                           ||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
13674R-7.in silico     61  ACCGCTCCGGCTGTGGTGGGCAGTGCCGCCTACGTGGCTCCC<span class="snp"><tt>-</tt></span>TACGCCTCC<span class="snp"><tt>-</tt></span>AGCTACA 120


13674R-7.IR_full       121 CCGCCAACTCGGTGGCCCACAGCGCCGCCT<span class="snp"><tt>N</tt></span>TCCCAGCTGCCTACACCGCCGCCTACAC<span class="snp"><tt>C</tt></span> 180
                           ||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>
13674R-7.in silico     121 CCGCCAACTCGGTGGCCCACAGCGCCGCCT<span class="snp"><tt>-</tt></span>TCCCAGCTGCCTACACCGCCGCCTACAC<span class="snp"><tt>T</tt></span> 180


13674R-7.IR_full       181 GCTCCCGTTGCTGC<span class="snp"><tt>A</tt></span>GCC<span class="snp"><tt>N</tt></span>TA<span class="snp"><tt>C</tt></span><span class="snp"><tt>G</tt></span>CCGCTCCAGTGGCTGCTGCTTATACCGCTCCAGTGGC 240
                           ||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||
13674R-7.in silico     181 GCTCCCGTTGCTGC<span class="snp"><tt>T</tt></span>GCC<span class="snp"><tt>-</tt></span>TA<span class="snp"><tt>T</tt></span><span class="snp"><tt>A</tt></span>CCGCTCCAGTGGCTGCTGCTTATACCGCTCCAGTGGC 240


13674R-7.IR_full       241 CGCTGCGTACG<span class="snp"><tt>N</tt></span>CGCCCCAGCTG<span class="snp"><tt>N</tt></span>CTATACCGCTGCCTACACCGCCCCCATTGCCCGTTA 300
                           |||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||
13674R-7.in silico     241 CGCTGCGTACG<span class="snp"><tt>C</tt></span>CGCCCCAGCTG<span class="snp"><tt>C</tt></span>CTATACCGCTGCCTACACCGCCCCCATTGCCCGTTA 300


13674R-7.IR_full       301 TGCCGCCACCCCCTTCGCAGCACCCATCGCCGCTCCCGTGGCTGCCGCCTACACCGCCCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13674R-7.in silico     301 TGCCGCCACCCCCTTCGCAGCACCCATCGCCGCTCCCGTGGCTGCCGCCTACACCGCCCC 360


13674R-7.IR_full       361 CA<span class="snp"><tt>N</tt></span>TCGCCGC<span class="snp"><tt>N</tt></span>GCTGCCCCAGTTCTGCTGAAGAA 394
                           ||<span class="snp"><tt>&nbsp;</tt></span>|||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||
13674R-7.in silico     361 CA<span class="snp"><tt>-</tt></span>TCGCCGC<span class="snp"><tt>C</tt></span>GCTGCCCCAGTTCTGCTGAAGAA 394

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	371	36	58	122	NM_139935.1	CG13674-RA (CG13674), mRNA
23.98	89	34	47	137	NM_139936.1	CG13678-RA (CG13678), mRNA
16.44	61	86	109	109	NM_139933.1	CG13679-RA, transcript variant A (CG13679), mRNA
16.44	61	86	109	109	NM_168260.1	CG13679-RB, transcript variant B (CG13679), mRNA
1.07	4	27	62	92	NM_140599.1	CG13068-RA (CG13068), mRNA
0.53	2	5	20	45	NM_144160.1	CG13066-RA (CG13066), mRNA
0.26	1	5	3	3	NM_167614.1	CG32549-RE, transcript variant E (CG32549), mRNA
0	0	8	10	15	NM_144161.1	CG13069-RA (CG13069), mRNA
0	0	7	37	112	NM_167394.1	CG32603-RA (CG32603), mRNA
0	0	5	30	52	NM_140600.1	CG13067-RA, transcript variant A (CG13067), mRNA
0	0	5	30	52	NM_206383.1	CG13067-RB, transcript variant B (CG13067), mRNA
0	0	4	5	28	NM_168096.1	CG32245-RB, transcript variant B (CG32245), mRNA
0	0	3	13	38	NM_140605.1	CG13047-RA (CG13047), mRNA
0	0	3	5	5	NM_206475.1	CG6959-RB, transcript variant B (CG6959), mRNA
0	0	3	5	5	NM_141865.1	CG6959-RA, transcript variant A (CG6959), mRNA
0	0	3	4	7	NM_001032052.1	CG33715-RE, transcript variant E (Msp-300), mRNA
0	0	3	3	17	NM_137564.2	CG7417-RA (Tab2), mRNA
0	0	3	3	16	NM_141072.2	CG7177-RA (CG7177), mRNA
0	0	3	3	7	NM_167282.2	CG1725-RA, transcript variant A (dlg1), mRNA
0	0	3	3	7	NM_206681.2	CG1725-RH, transcript variant H (dlg1), mRNA
0	0	3	3	7	NM_206683.2	CG1725-RB, transcript variant B (dlg1), mRNA
0	0	3	3	5	NM_206684.2	CG1725-RE, transcript variant E (dlg1), mRNA
0	0	3	3	5	NM_206682.2	CG1725-RG, transcript variant G (dlg1), mRNA
0	0	3	3	5	NM_078565.3	CG1725-RD, transcript variant D (dlg1), mRNA
0	0	3	3	4	NM_164688.1	CG31640-RA (CG31640), mRNA
0	0	3	2	2	NM_206531.1	CG5730-RC, transcript variant C (AnnIX), mRNA
0	0	3	2	2	NM_057256.3	CG5730-RA, transcript variant A (AnnIX), mRNA
0	0	3	2	2	NM_206530.1	CG5730-RD, transcript variant D (AnnIX), mRNA
0	0	3	2	2	NM_057255.2	CG5730-RB, transcript variant B (AnnIX), mRNA
0	0	2	15	26	NM_140610.2	CG13044-RA (CG13044), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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