NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

13670R-2

Symbol

CG13670

Full Name

CG13670

CG No

CG13670

Old CG No

CG13670

Synonyms

CG13670

Accession No (Link to NCBI)

NM_139950.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

semi-lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCGCATAACCAGCACAGCTGGA

Primer Seq. 3'

aatctagaggtaccCCGTGGCCGTGCAGAGTTTTC

Predicted Fragment Size

IR fragment full Seq

0001 CGCATAACCA GCACAGCTGG AGATGCCAAT GGACCTGGCG TTGGACCAAA AGCACGGACT 
0061 TCAANCTCGC TGGCGCTTCC GCATTgCGTT GCGAAGAGAG GCACGCTTCG GCCAGGCGGT 
0121 GCGGCAACTC AAAGATTTGT TGCCACTGTT GGCGCTAATT GCTGGATGCG GAATGATCGG 
0181 TGCATGCGCA GCGNTTGGCT ACTCCTATGC AAGATTATTC GAGGNTCCGN TGGTGGGNCC 
0241 GNAGCATCTG NTCACCGTCC ACGACGGACG CACCGTGGAC TATGTGGCCC GTCCCGANTA 
0301 cAGNTTCGCC TATGGAGTCG AGGATGGCAN AGACGCGCGT GCTGCAGAAT CGCAAGGAGA 
0361 CGCGCAACGG GGACGAGGTG AGAGGAGTGT ACAGCGTAGT CGATCCGGAT GGCACCTTGC 
0421 GTGTGGTTAA ATACACGGCC GACGATgNCA ACGGCTTCCA AGCCGAGGTC ATTACCAATG 
0481 GTGTGAAAAC TCTGCAcggC CACGG

in silico PCR Fragment

0001 CGCATAACCA GCACAGCTGG AGATGCCAAT GGACCTGGCG TTGGACCAAA AGCACGGACT 
0061 TCAA-CTCGC TGGCGCTTCC GCATTGCGTT GCGAAGAGAG GCACGCTTCG GCCAGGCGGT 
0121 GCGGCAACTC AAAGATTTGT TGCCACTGTT GGCGCTAATT GCTGGATGCG GAATGATCGG 
0181 TGCATGCGCA GCGGTTGGCT ACTCCTATGC AAG---ATTC GAGGGTCCGG TGGTGGGGCC 
0241 GGAGCATCTG GTCACCGTCC ACGACGGACG CACCGTGGAC TATGTGGCCC GTCCCGAGTA 
0301 CAGCTTCGCC TATGGAGTCG AGGATGGCA- AGACGCGCGT GCTGCAGAAT CGCAAGGAGA 
0361 CGCGCAACGG GGACGAGGTG AGAGGAGTGT ACAGCGTAGT CGATCCGGAT GGCACCTTGC 
0421 GTGTGGTTAA ATACACGGCC GACGATGCCA ACGGCTTCCA AGCCGAGGTC ATTACCAATG 
0481 GTGTGAAAAC TCTGCACGGC CACGG

Assemble Data

<PRE>

13670R-2.IR_full       1   CGCATAACCAGCACAGCTGGAGATGCCAATGGACCTGGCGTTGGACCAAAAGCACGGACT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13670R-2.in silico     1   CGCATAACCAGCACAGCTGGAGATGCCAATGGACCTGGCGTTGGACCAAAAGCACGGACT 60


13670R-2.IR_full       61  TCAA<span class="snp"><tt>N</tt></span>CTCGCTGGCGCTTCCGCATTGCGTTGCGAAGAGAGGCACGCTTCGGCCAGGCGGT 120
                           ||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||||||||||
13670R-2.in silico     61  TCAA<span class="snp"><tt>-</tt></span>CTCGCTGGCGCTTCCGCATTGCGTTGCGAAGAGAGGCACGCTTCGGCCAGGCGGT 120


13670R-2.IR_full       121 GCGGCAACTCAAAGATTTGTTGCCACTGTTGGCGCTAATTGCTGGATGCGGAATGATCGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13670R-2.in silico     121 GCGGCAACTCAAAGATTTGTTGCCACTGTTGGCGCTAATTGCTGGATGCGGAATGATCGG 180


13670R-2.IR_full       181 TGCATGCGCAGCG<span class="snp"><tt>N</tt></span>TTGGCTACTCCTATGCAAG<span class="snp"><tt>A</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>T</tt></span>ATTCGAGG<span class="snp"><tt>N</tt></span>TCCG<span class="snp"><tt>N</tt></span>TGGTGGG<span class="snp"><tt>N</tt></span>CC 240
                           |||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>||||<span class="snp"><tt>&nbsp;</tt></span>|||||||<span class="snp"><tt>&nbsp;</tt></span>||
13670R-2.in silico     181 TGCATGCGCAGCG<span class="snp"><tt>G</tt></span>TTGGCTACTCCTATGCAAG<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>ATTCGAGG<span class="snp"><tt>G</tt></span>TCCG<span class="snp"><tt>G</tt></span>TGGTGGG<span class="snp"><tt>G</tt></span>CC 240


13670R-2.IR_full       241 G<span class="snp"><tt>N</tt></span>AGCATCTG<span class="snp"><tt>N</tt></span>TCACCGTCCACGACGGACGCACCGTGGACTATGTGGCCCGTCCCGA<span class="snp"><tt>N</tt></span>TA 300
                           |<span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||
13670R-2.in silico     241 G<span class="snp"><tt>G</tt></span>AGCATCTG<span class="snp"><tt>G</tt></span>TCACCGTCCACGACGGACGCACCGTGGACTATGTGGCCCGTCCCGA<span class="snp"><tt>G</tt></span>TA 300


13670R-2.IR_full       301 CAG<span class="snp"><tt>N</tt></span>TTCGCCTATGGAGTCGAGGATGGCA<span class="snp"><tt>N</tt></span>AGACGCGCGTGCTGCAGAATCGCAAGGAGA 360
                           |||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
13670R-2.in silico     301 CAG<span class="snp"><tt>C</tt></span>TTCGCCTATGGAGTCGAGGATGGCA<span class="snp"><tt>-</tt></span>AGACGCGCGTGCTGCAGAATCGCAAGGAGA 360


13670R-2.IR_full       361 CGCGCAACGGGGACGAGGTGAGAGGAGTGTACAGCGTAGTCGATCCGGATGGCACCTTGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13670R-2.in silico     361 CGCGCAACGGGGACGAGGTGAGAGGAGTGTACAGCGTAGTCGATCCGGATGGCACCTTGC 420


13670R-2.IR_full       421 GTGTGGTTAAATACACGGCCGACGATG<span class="snp"><tt>N</tt></span>CAACGGCTTCCAAGCCGAGGTCATTACCAATG 480
                           |||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||
13670R-2.in silico     421 GTGTGGTTAAATACACGGCCGACGATG<span class="snp"><tt>C</tt></span>CAACGGCTTCCAAGCCGAGGTCATTACCAATG 480


13670R-2.IR_full       481 GTGTGAAAACTCTGCACGGCCACGG 505
                           |||||||||||||||||||||||||
13670R-2.in silico     481 GTGTGAAAACTCTGCACGGCCACGG 505

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_139950.1	CG13670-RA (CG13670), mRNA
0.2	1	3	3	8	NM_166385.2	CG8908-RA, transcript variant A (CG8908), mRNA
0.2	1	3	3	8	NM_137620.2	CG8908-RB, transcript variant B (CG8908), mRNA
0	0	2	4	11	NM_140626.1	CG12255-RA (CG12255), mRNA
0	0	2	3	5	NM_140866.1	CG9299-RA (CG9299), mRNA
0	0	2	3	2	NM_140295.2	CG6811-RA (RhoGAP68F), mRNA
0	0	2	2	2	NM_139753.1	CG10477-RA (CG10477), mRNA
0	0	1	3	7	NM_142925.1	CG10217-RA, transcript variant A (CG10217), mRNA
0	0	1	3	7	NM_170091.1	CG10217-RB, transcript variant B (CG10217), mRNA
0	0	1	3	2	NM_079192.2	CG15811-RA (Rop), mRNA
0	0	1	2	4	NM_141216.2	CG14650-RA (CG14650), mRNA
0	0	1	2	4	NM_132472.1	CG12625-RA (CG12625), mRNA
0	0	1	2	4	NM_135804.2	CG7099-RA (CG7099), mRNA
0	0	1	2	2	NM_057260.3	CG10561-RA (CG10561), mRNA
0	0	1	2	2	NM_080025.1	CG11387-RA, transcript variant A (ct), mRNA
0	0	1	2	2	NM_167130.1	CG11387-RB, transcript variant B (ct), mRNA
0	0	0	7	7	NM_169852.1	CG3772-RA (cry), mRNA
0	0	0	5	10	NM_078601.2	CG9533-RA (rut), mRNA
0	0	0	5	7	NM_001014622.1	CG33555-RA, transcript variant A (btsz), mRNA
0	0	0	5	7	NM_001014625.1	CG33555-RB, transcript variant B (btsz), mRNA
0	0	0	5	3	NM_167475.1	CG17336-RA, transcript variant A (Lcch3), mRNA
0	0	0	5	2	NM_206746.1	CG17336-RC, transcript variant C (Lcch3), mRNA
0	0	0	5	2	NM_078632.2	CG17336-RB, transcript variant B (Lcch3), mRNA
0	0	0	4	7	NM_140266.1	CG5718-RA (CG5718), mRNA
0	0	0	4	6	NM_001043216.1	CG34113-RO, transcript variant O (CG34113), mRNA
0	0	0	4	5	NM_130604.2	CG17766-RA (CG17766), mRNA
0	0	0	3	8	NM_079876.2	CG1483-RA, transcript variant A (Map205), mRNA
0	0	0	3	8	NM_170586.1	CG1483-RB, transcript variant B (Map205), mRNA
0	0	0	3	6	NM_134516.2	CG32529-RA, transcript variant A (CG32529), mRNA
0	0	0	3	5	NM_206440.1	CG31481-RC, transcript variant C (pb), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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