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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

13627R-3

Symbol

CG13627

Full Name

CG13627

CG No

CG13627

Old CG No

CG13627

Synonyms

CG13627

Accession No (Link to NCBI)

NM_143022.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.<br> Zyxin links fat signaling to the hippo pathway.<br> PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCCATTTCTGCTGTTCGCACT

Primer Seq. 3'

aatctagaggtaccTGTCGTATCCGAATGTGGGTT

Predicted Fragment Size

IR fragment full Seq

0001 GCCATTTTNT CTGCTGTNTC GCACTGCTCC TGGCGCTGGC TAACTGCGAG CAGCGTCCAC 
0061 TGGTTTTCAC CAACTGGCCA CCGAAGAGCT CCAAGCAGCT GAGTCGTCCC GCCATTGGAG 
0121 CTGGAGGTCC TGGATCCGCT GGAGCGCCTG GAAAGCAGCG CACGCTGGTC GTCCAAATAC 
0181 GCAGCGATCA ACCGGTGCCC TTGGTACTGA AGGGTCGTCC TGGACATGGA GCGCACAGCC 
0241 ATGCCGCCCA TCTGGCCCAT CCGCATTCCG TGCATCCTGC ACATGTCCTG TCGCACGCCC 
0301 ATTCACATGC CCATCTACAC TCGCATCCGC TGATCCATGT GCCACATCAG TACCAGCATC 
0361 AGCACCTACG CGGACCACCT CGTCCCATGA AGCTGAGTGG TCCGTCGCCC GTGTACCTGA 
0421 AACCAGCTGG ATCTCTAAGG AAACCACTGA AGATCAAACT GAACAATGTG AAGCCCAAGA 
0481 CGAAACCCAC ATTCGGATAC GACA

in silico PCR Fragment

0001 GCCATT---T CTGCTGT-TC GCACTGCTCC TGGCGCTGGC TAACTGCGAG CAGCGTCCAC 
0061 TGGTTTTCAC CAACTGGCCA CCGAAGAGCT CCAAGCAGCT GAGTCGTCCC GCCATTGGAG 
0121 CTGGAGGTCC TGGATCCGCT GGAGCGCCTG GAAAGCAGCG CACGCTGGTC GTCCAAATAC 
0181 GCAGCGATCA ACCGGTGCCC TTGGTACTGA AGGGTCGTCC TGGACATGGA GCGCACAGCC 
0241 ATGCCGCCCA TCTGGCCCAT CCGCATTCCG TGCATCCTGC ACATGTCCTG TCGCACGCCC 
0301 ATTCACATGC CCATCTACAC TCGCATCCGC TGATCCATGT GCCACATCAG TACCAGCATC 
0361 AGCACCTACG CGGACCACCT CGTCCCATGA AGCTGAGTGG TCCGTCGCCC GTGTACCTGA 
0421 AACCAGCTGG ATCTCTAAGG AAACCACTGA AGATCAAACT GAACAATGTG AAGCCCAAGA 
0481 CGAAACCCAC ATTCGGATAC GACA

Assemble Data

<PRE>

13627R-3.IR_full       1   GCCATT<span class="snp"><tt>T</tt></span><span class="snp"><tt>T</tt></span><span class="snp"><tt>N</tt></span>TCTGCTGT<span class="snp"><tt>N</tt></span>TCGCACTGCTCCTGGCGCTGGCTAACTGCGAGCAGCGTCCAC 60
                           ||||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||
13627R-3.in silico     1   GCCATT<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>TCTGCTGT<span class="snp"><tt>-</tt></span>TCGCACTGCTCCTGGCGCTGGCTAACTGCGAGCAGCGTCCAC 60


13627R-3.IR_full       61  TGGTTTTCACCAACTGGCCACCGAAGAGCTCCAAGCAGCTGAGTCGTCCCGCCATTGGAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13627R-3.in silico     61  TGGTTTTCACCAACTGGCCACCGAAGAGCTCCAAGCAGCTGAGTCGTCCCGCCATTGGAG 120


13627R-3.IR_full       121 CTGGAGGTCCTGGATCCGCTGGAGCGCCTGGAAAGCAGCGCACGCTGGTCGTCCAAATAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13627R-3.in silico     121 CTGGAGGTCCTGGATCCGCTGGAGCGCCTGGAAAGCAGCGCACGCTGGTCGTCCAAATAC 180


13627R-3.IR_full       181 GCAGCGATCAACCGGTGCCCTTGGTACTGAAGGGTCGTCCTGGACATGGAGCGCACAGCC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13627R-3.in silico     181 GCAGCGATCAACCGGTGCCCTTGGTACTGAAGGGTCGTCCTGGACATGGAGCGCACAGCC 240


13627R-3.IR_full       241 ATGCCGCCCATCTGGCCCATCCGCATTCCGTGCATCCTGCACATGTCCTGTCGCACGCCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13627R-3.in silico     241 ATGCCGCCCATCTGGCCCATCCGCATTCCGTGCATCCTGCACATGTCCTGTCGCACGCCC 300


13627R-3.IR_full       301 ATTCACATGCCCATCTACACTCGCATCCGCTGATCCATGTGCCACATCAGTACCAGCATC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13627R-3.in silico     301 ATTCACATGCCCATCTACACTCGCATCCGCTGATCCATGTGCCACATCAGTACCAGCATC 360


13627R-3.IR_full       361 AGCACCTACGCGGACCACCTCGTCCCATGAAGCTGAGTGGTCCGTCGCCCGTGTACCTGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13627R-3.in silico     361 AGCACCTACGCGGACCACCTCGTCCCATGAAGCTGAGTGGTCCGTCGCCCGTGTACCTGA 420


13627R-3.IR_full       421 AACCAGCTGGATCTCTAAGGAAACCACTGAAGATCAAACTGAACAATGTGAAGCCCAAGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13627R-3.in silico     421 AACCAGCTGGATCTCTAAGGAAACCACTGAAGATCAAACTGAACAATGTGAAGCCCAAGA 480


13627R-3.IR_full       481 CGAAACCCACATTCGGATACGACA 504
                           ||||||||||||||||||||||||
13627R-3.in silico     481 CGAAACCCACATTCGGATACGACA 504

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	1	7	NM_143022.2	CG13627-RA, transcript variant A (CG13627), mRNA
99.79	481	1	1	7	NM_170166.2	CG13627-RB, transcript variant B (CG13627), mRNA
0	0	5	5	15	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	5	5	15	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	5	5	15	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	4	6	12	NM_130635.2	CG3078-RA (CG3078), mRNA
0	0	3	6	23	NM_132538.1	CG15737-RA (CG15737), mRNA
0	0	2	6	17	NM_078797.2	CG13109-RA (tai), mRNA
0	0	2	5	14	NM_169245.2	CG31349-RA, transcript variant A (pyd), mRNA
0	0	2	5	9	NM_169246.2	CG31349-RC, transcript variant C (pyd), mRNA
0	0	2	5	9	NM_176426.2	CG31349-RF, transcript variant F (pyd), mRNA
0	0	2	5	9	NM_057349.4	CG31349-RB, transcript variant B (pyd), mRNA
0	0	2	4	9	NM_132441.1	CG2174-RA, transcript variant A (Myo10A), mRNA
0	0	2	4	9	NM_001042804.1	CG2174-RB, transcript variant B (Myo10A), mRNA
0	0	2	4	2	NM_139849.2	CG8598-RA, transcript variant A (eco), mRNA
0	0	2	4	2	NM_168201.2	CG8598-RB, transcript variant B (eco), mRNA
0	0	2	3	6	NM_169223.2	CG31369-RA (PQBP-1), mRNA
0	0	2	3	6	NM_167625.1	CG6500-RA, transcript variant A (Bx), mRNA
0	0	2	3	6	NM_176758.1	CG6500-RC, transcript variant C (Bx), mRNA
0	0	2	3	6	NM_078678.2	CG6500-RB, transcript variant B (Bx), mRNA
0	0	2	2	4	NM_079002.2	CG3905-RA (Su(z)2), mRNA
0	0	2	2	4	NM_136598.2	CG8196-RA (Ance-4), mRNA
0	0	1	5	7	NM_169455.1	CG4795-RB, transcript variant B (Cpn), mRNA
0	0	1	5	7	NM_169454.1	CG4795-RA, transcript variant A (Cpn), mRNA
0	0	1	4	12	NM_165217.1	CG6667-RA, transcript variant A (dl), mRNA
0	0	1	4	12	NM_165218.1	CG6667-RB, transcript variant B (dl), mRNA
0	0	1	4	8	NM_168879.2	CG9936-RC, transcript variant C (skd), mRNA
0	0	1	4	8	NM_165556.1	CG30497-RC, transcript variant C (CG30497), mRNA
0	0	1	4	8	NM_165555.1	CG30497-RB, transcript variant B (CG30497), mRNA
0	0	1	4	8	NM_136477.2	CG30497-RA, transcript variant A (CG30497), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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