|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13472R-1 |
| Symbol |
CG13472 |
Full Name |
CG13472 |
| CG No |
CG13472 |
Old CG No |
CG13472 |
| Synonyms |
CG13472 |
| Accession No (Link to NCBI) |
NM_140467.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Park J, Lee J, Kim JH, Lee J, Park H, Lim C.<br> ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD.<br> Nucleic Acids Res (2021) 49(19) 11294-11311
[
PubMed ID = 34551427
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.<br> Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.<br> Nature (2009) 458(7241) 987-92
[
PubMed ID = 19363474
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgACCTGACAGAGGAGGGCCTTA |
| Primer Seq. 3' |
aatctagaggtaccTGGCGCTGATGTCCCATTCAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ACCTGACAGA GGAgGGCCTT AAAATCGTGA CAACAGCCGT CGGCAGCGAG GATGTCCGGA
0061 AAATAGTCAA TGATGCACTG AATCGAGATC TGCGCGATAT TGGCGGTGGC GCACTGNCCG
0121 CGAAACGCGA AGATGCCACT TTGCCCGGTA AAATTGTGCT GCAGGTTCAG CGTGTGCGAA
0181 ATATTGCCGC TCCCAAGGCG AATGAGGAAT CGAAGGCGGC GCCACGCCTC CNTTCAGTTG
0241 GATCTGAGTG ATGGGCAGAA TTCCATAAAG GCCTTGGAGC TGGAGCCAGT TCCCCAACTG
0301 AATCTCAATG TGGCTCCCGG AAGCAAGATC TATTTCAAGG CCGAGAAACT GCAGCTTATG
0361 CAGGGATTCC TGCTGCTAAA GTCCAGTGAA ATTCAGCTGC TCGGCGGCCG TGTGGATGCA
0421 CTCTACGAGA AATGGGATCT GGCCAGGACC ATGATGAGAT ATGCNCGCAG TGGGCGCCCC
0481 TTGAATGGGA CATCAGCGCC A |
| in silico PCR Fragment |
0001 ACCTGACAGA GGAGGGCCTT AAAATCGTGA CAACAGCCGT CGGCAGCGAG GATGTCCGGA
0061 AAATAGTCAA TGATGCACTG AATCGAGATC TGCGCGATAT TGGCGGTGGC GCACTGCCCG
0121 CGAAACGCGA AGATGCCACT TTGCCCGGTA AAATTGTGCT GCAGGTTCAG CGTGTGCGAA
0181 ATATTGCCGC TCCCAAGGCG AATGAGGAAT CGAAGGCGGC GCCACGCCTC C-TTCAGTTG
0241 GATCTGAGTG ATGGGCAGAA TTCCATAAAG GCCTTGGAGC TGGAGCCAGT TCCCCAACTG
0301 AATCTCAATG TGGCTCCCGG AAGCAAGATC TATTTCAAGG CCGAGAAACT GCAGCTTATG
0361 CAGGGATTCC TGCTGCTAAA GTCCAGTGAA ATTCAGCTGC TCGGCGGCCG TGTGGATGCA
0421 CTCTACGAGA AATGGGATCT GGCCAGGACC ATGATGAGAT ATGCGCGCAG TGGGCGCCCC
0481 TTGAATGGGA CATCAGCGCC A |
| Assemble Data |
<PRE>
13472R-1.IR_full 1 ACCTGACAGAGGAGGGCCTTAAAATCGTGACAACAGCCGTCGGCAGCGAGGATGTCCGGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13472R-1.in silico 1 ACCTGACAGAGGAGGGCCTTAAAATCGTGACAACAGCCGTCGGCAGCGAGGATGTCCGGA 60
13472R-1.IR_full 61 AAATAGTCAATGATGCACTGAATCGAGATCTGCGCGATATTGGCGGTGGCGCACTG<span class="snp"><tt>N</tt></span>CCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||
13472R-1.in silico 61 AAATAGTCAATGATGCACTGAATCGAGATCTGCGCGATATTGGCGGTGGCGCACTG<span class="snp"><tt>C</tt></span>CCG 120
13472R-1.IR_full 121 CGAAACGCGAAGATGCCACTTTGCCCGGTAAAATTGTGCTGCAGGTTCAGCGTGTGCGAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13472R-1.in silico 121 CGAAACGCGAAGATGCCACTTTGCCCGGTAAAATTGTGCTGCAGGTTCAGCGTGTGCGAA 180
13472R-1.IR_full 181 ATATTGCCGCTCCCAAGGCGAATGAGGAATCGAAGGCGGCGCCACGCCTCC<span class="snp"><tt>N</tt></span>TTCAGTTG 240
|||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||
13472R-1.in silico 181 ATATTGCCGCTCCCAAGGCGAATGAGGAATCGAAGGCGGCGCCACGCCTCC<span class="snp"><tt>-</tt></span>TTCAGTTG 240
13472R-1.IR_full 241 GATCTGAGTGATGGGCAGAATTCCATAAAGGCCTTGGAGCTGGAGCCAGTTCCCCAACTG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13472R-1.in silico 241 GATCTGAGTGATGGGCAGAATTCCATAAAGGCCTTGGAGCTGGAGCCAGTTCCCCAACTG 300
13472R-1.IR_full 301 AATCTCAATGTGGCTCCCGGAAGCAAGATCTATTTCAAGGCCGAGAAACTGCAGCTTATG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13472R-1.in silico 301 AATCTCAATGTGGCTCCCGGAAGCAAGATCTATTTCAAGGCCGAGAAACTGCAGCTTATG 360
13472R-1.IR_full 361 CAGGGATTCCTGCTGCTAAAGTCCAGTGAAATTCAGCTGCTCGGCGGCCGTGTGGATGCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13472R-1.in silico 361 CAGGGATTCCTGCTGCTAAAGTCCAGTGAAATTCAGCTGCTCGGCGGCCGTGTGGATGCA 420
13472R-1.IR_full 421 CTCTACGAGAAATGGGATCTGGCCAGGACCATGATGAGATATGC<span class="snp"><tt>N</tt></span>CGCAGTGGGCGCCCC 480
||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||
13472R-1.in silico 421 CTCTACGAGAAATGGGATCTGGCCAGGACCATGATGAGATATGC<span class="snp"><tt>G</tt></span>CGCAGTGGGCGCCCC 480
13472R-1.IR_full 481 TTGAATGGGACATCAGCGCCA 501
|||||||||||||||||||||
13472R-1.in silico 481 TTGAATGGGACATCAGCGCCA 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140467.2 |
CG13472-RA (CG13472), mRNA |
| 0.2 |
1 |
2 |
4 |
2 |
NM_058140.3 |
CG10033-RB, transcript variant B (for), mRNA |
| 0.2 |
1 |
2 |
4 |
2 |
NM_205906.1 |
CG10033-RH, transcript variant H (for), mRNA |
| 0.2 |
1 |
2 |
4 |
2 |
NM_205904.1 |
CG10033-RI, transcript variant I (for), mRNA |
| 0.2 |
1 |
2 |
4 |
2 |
NM_058139.3 |
CG10033-RA, transcript variant A (for), mRNA |
| 0 |
0 |
2 |
6 |
5 |
NM_136502.2 |
CG14762-RA (CG14762), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_166524.1 |
CG3413-RA, transcript variant A (wdp), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_166525.1 |
CG3413-RC, transcript variant C (wdp), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_166526.1 |
CG3413-RD, transcript variant D (wdp), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_137817.2 |
CG3413-RB, transcript variant B (wdp), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_206560.1 |
CG13614-RB, transcript variant B (CG13614), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_142999.2 |
CG13614-RA, transcript variant A (CG13614), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166148.1 |
CG8430-RB, transcript variant B (Got1), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137242.2 |
CG8430-RA, transcript variant A (Got1), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079845.2 |
CG7951-RA (sima), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_169386.1 |
CG17228-RA, transcript variant A (pros), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_176459.1 |
CG17228-RD, transcript variant D (pros), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079593.3 |
CG17228-RC, transcript variant C (pros), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_134507.1 |
CG14234-RA (CG14234), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141948.1 |
CG5333-RA (trus), mRNA |
| 0 |
0 |
1 |
2 |
13 |
NM_143300.1 |
CG3339-RA, transcript variant A (CG3339), mRNA |
| 0 |
0 |
1 |
2 |
13 |
NM_001043297.1 |
CG3339-RB, transcript variant B (CG3339), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_135666.1 |
CG6589-RA (CG6589), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_166047.1 |
CG8603-RB, transcript variant B (CG8603), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_137108.2 |
CG8603-RA, transcript variant A (CG8603), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_079503.2 |
CG9761-RA (Nep2), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_137385.1 |
CG18432-RA (CG18432), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_168687.1 |
CG9674-RC, transcript variant C (CG9674), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_132959.2 |
CG8949-RA (CG8949), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_168686.1 |
CG9674-RB, transcript variant B (CG9674), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
