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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13470R-3 
 Symbol CG33260  Full Name CG33260 
 CG No CG33260  Old CG No CG13470 
 Synonyms CG13470, CG33260 
 Accession No (Link to NCBI) NM_001043140.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGAGCAAACATCGCATAATGG 
 Primer Seq. 3' 
aatctagaggtaccGAAAACGCGGTATTTCAGGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGAGCAAACA TCGCATAATG GGTGCTCCAG TGGGCACAGC GAACACAAAA GGCTGGAGTC 
0061 CTAATCAATC CACTCTGCCC GTGGAGCTCA GTAAATTTGA AACCCAATTG CTTTTGGATG 
0121 AGAATATAGC AAAGTTAGTT GATAAATCCA AAGATCTAAA GGCTAAACCC ACTGCCGAGA 
0181 AACTTGAGGA GTGCAAGGCG GATTTTGAAT CCCGGTTACT GGGACAGGAG GAAGCATTGA 
0241 AAACGGAGAA ACTACGGGAA ACTGAACGAT ACATGGATAA GATTCTGACG GGAAAACGCA 
0301 AGAAGTTGCT CAAGCAGGGC AAAAATGAAT TGGCTGCCGC TCTGACTGAT AAGGATGTAC 
0361 TATTGGAGAT GTCTGTGAAT TTCAAATTCG ATCGCCAAAA TGCCTTGCTG GAGGTGCATT 
0421 GTGAACATCC CACGGATCAC TCTGCGCAAA TAGTTACCGG TCCCATTGTG CACACCAATT 
0481 CCCTGAAATA CCGCGTTTTC  
 in silico PCR Fragment 
0001 GGAGCAAACA TCGCATAATG GGTGCTCCAG TGGGCACAGC GAACACAAAA GGCTGGAGTC 
0061 CTAATCAATC CACTCTGCCC GTGGAGCTCA GTAAATTTGA AACCCAATTG CTTTTGGATG 
0121 AGAATATAGC AAAGTTAGTT GATAAATCCA AAGATCTAAA GGCTAAACCC ACTGCCGAGA 
0181 AACTTGAGGA GTGCAAGGCG GATTTTGAAT CCCGGTTACT GGGACAGGAG GAAGCATTGA 
0241 AAACGGAGAA ACTACGGGAA ACTGAACGAT ACATGGATAA GATTCTGACG GGAAAACGCA 
0301 AGAAGTTGCT CAAGCAGGGC AAAAATGAAT TGGCTGCCGC TCTGACTGAT AAGGATGTAC 
0361 TATTGGAGAT GTCTGTGAAT TTCAAATTCG ATCGCCAAAA TGCCTTGCTG GAGGTGCATT 
0421 GTGAACATCC CACGGATCAC TCTGCGCAAA TAGTTACCGG TCCCATTGTG CACACCAATT 
0481 CCCTGAAATA CCGCGTTTTC  
 Assemble Data 
<PRE>

13470R-3.IR_full       1   GGAGCAAACATCGCATAATGGGTGCTCCAGTGGGCACAGCGAACACAAAAGGCTGGAGTC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13470R-3.in silico     1   GGAGCAAACATCGCATAATGGGTGCTCCAGTGGGCACAGCGAACACAAAAGGCTGGAGTC 60


13470R-3.IR_full       61  CTAATCAATCCACTCTGCCCGTGGAGCTCAGTAAATTTGAAACCCAATTGCTTTTGGATG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13470R-3.in silico     61  CTAATCAATCCACTCTGCCCGTGGAGCTCAGTAAATTTGAAACCCAATTGCTTTTGGATG 120


13470R-3.IR_full       121 AGAATATAGCAAAGTTAGTTGATAAATCCAAAGATCTAAAGGCTAAACCCACTGCCGAGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13470R-3.in silico     121 AGAATATAGCAAAGTTAGTTGATAAATCCAAAGATCTAAAGGCTAAACCCACTGCCGAGA 180


13470R-3.IR_full       181 AACTTGAGGAGTGCAAGGCGGATTTTGAATCCCGGTTACTGGGACAGGAGGAAGCATTGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13470R-3.in silico     181 AACTTGAGGAGTGCAAGGCGGATTTTGAATCCCGGTTACTGGGACAGGAGGAAGCATTGA 240


13470R-3.IR_full       241 AAACGGAGAAACTACGGGAAACTGAACGATACATGGATAAGATTCTGACGGGAAAACGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13470R-3.in silico     241 AAACGGAGAAACTACGGGAAACTGAACGATACATGGATAAGATTCTGACGGGAAAACGCA 300


13470R-3.IR_full       301 AGAAGTTGCTCAAGCAGGGCAAAAATGAATTGGCTGCCGCTCTGACTGATAAGGATGTAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13470R-3.in silico     301 AGAAGTTGCTCAAGCAGGGCAAAAATGAATTGGCTGCCGCTCTGACTGATAAGGATGTAC 360


13470R-3.IR_full       361 TATTGGAGATGTCTGTGAATTTCAAATTCGATCGCCAAAATGCCTTGCTGGAGGTGCATT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13470R-3.in silico     361 TATTGGAGATGTCTGTGAATTTCAAATTCGATCGCCAAAATGCCTTGCTGGAGGTGCATT 420


13470R-3.IR_full       421 GTGAACATCCCACGGATCACTCTGCGCAAATAGTTACCGGTCCCATTGTGCACACCAATT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13470R-3.in silico     421 GTGAACATCCCACGGATCACTCTGCGCAAATAGTTACCGGTCCCATTGTGCACACCAATT 480


13470R-3.IR_full       481 CCCTGAAATACCGCGTTTTC 500
                           ||||||||||||||||||||
13470R-3.in silico     481 CCCTGAAATACCGCGTTTTC 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_001043140.1  CG33260-RB (CG33260), mRNA 
25.72   124  0  0  5  NM_206360.1  CG33261-RF, transcript variant F (Trl), mRNA 
0   0  5  4  2  NM_168064.2  CG14998-RA, transcript variant A (CG14998), mRNA 
0   0  5  4  2  NM_168063.2  CG14998-RD, transcript variant D (CG14998), mRNA 
0   0  5  4  2  NM_168062.2  CG14998-RE, transcript variant E (CG14998), mRNA 
0   0  5  4  2  NM_168061.2  CG14998-RB, transcript variant B (CG14998), mRNA 
0   0  5  4  2  NM_139604.2  CG14998-RC, transcript variant C (CG14998), mRNA 
0   0  5  2  3  NM_132819.2  CG8105-RA (CG8105), mRNA 
0   0  3  5  4  NM_141429.2  CG1984-RA (djl), mRNA 
0   0  3  3  3  NM_137847.2  CG4207-RA (bonsai), mRNA 
0   0  1  7  5  NM_140068.2  CG3428-RA (CG3428), mRNA 
0   0  1  5  2  NM_170177.2  CG3744-RB, transcript variant B (CG3744), mRNA 
0   0  1  5  2  NM_206569.1  CG3744-RC, transcript variant C (CG3744), mRNA 
0   0  1  5  2  NM_143047.2  CG3744-RA, transcript variant A (CG3744), mRNA 
0   0  1  4  5  NM_135224.1  CG11201-RA (CG11201), mRNA 
0   0  1  4  3  NM_143282.2  CG5880-RA (CG5880), mRNA 
0   0  1  4  3  NM_142993.2  CG17785-RA (Golgin84), mRNA 
0   0  1  4  2  NM_130705.1  CG10804-RB, transcript variant B (CG10804), mRNA 
0   0  1  4  2  NM_166975.1  CG10804-RA, transcript variant A (CG10804), mRNA 
0   0  1  4  2  NM_140730.2  CG13732-RA (CG13732), mRNA 
0   0  1  3  4  NM_141611.1  CG9746-RA (CG9746), mRNA 
0   0  1  2  6  NM_168254.1  CG7915-RB, transcript variant B (Ect4), mRNA 
0   0  1  2  6  NM_001043129.1  CG7915-RC, transcript variant C (Ect4), mRNA 
0   0  1  2  3  NM_132019.1  CG4096-RA (CG4096), mRNA 
0   0  1  2  2  NM_132912.1  CG4521-RA (mthl1), mRNA 
0   0  1  2  2  NM_167239.2  CG32677-RA (CG32677), mRNA 
0   0  0  6  4  NM_080495.3  CG12047-RC, transcript variant C (mud), mRNA 
0   0  0  6  4  NM_167400.3  CG12047-RA, transcript variant A (mud), mRNA 
0   0  0  6  4  NM_167399.4  CG12047-RB, transcript variant B (mud), mRNA 
0   0  0  5  8  NM_176557.1  CG33106-RB, transcript variant B (mask), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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