|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13459R-2 |
| Symbol |
cp309 |
Full Name |
cp309 |
| CG No |
CG33957 |
Old CG No |
CG13459 |
| Synonyms |
D-PLP, CG6735, CG18648, CG13459, d-plp, CP309, AKAP450, l(3)s2172, CG33957, cp309, PACT, dplp |
| Accession No (Link to NCBI) |
NM_001032402.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male pupal semi-lethal, female late pupal semi-lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCACGGTGCCAGTGTAAGCTAT |
| Primer Seq. 3' |
aatctagaggtaccGTGGTCGTGGTTGTCCGTTGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CACGGTGCCA GTGTAAGCTA TAAGGCGATC GAAGCGGCGA CTCTCGAAGA ATTGCCAGCA
0061 ACATCCGTGG CAACAGCAAC ATCCACGGCT GCAACATCAC GCGCCACTGG AGGAGCAGCA
0121 GCAACTTGTG TGGACGTTGA GTTCATACCC ACGCTAAATA TCATATTTGA GAANGCCGAC
0181 TTCGTCTAGT GAGCAACTAC AGTGCGCCGA GAAATCGGAA CATGTGGCCG GTGGTGTTTT
0241 CGATAAGGAA CCAGCAGATG ATTGTGACTC CGACAATGAT TCCACGCGCA CATACATAAT
0301 CTCGCGAAGC AGTTGGACGG GGGTAACATC AAGTAGCAGC ACCCCATATG CGGTTACATC
0361 CACGGATACC GCCGAATTAC TCAGGCGCCA GAACAATCTC AGCCTTACGG AGGAGGATCA
0421 ATCGGTGTCG TCGTTTAGCA TCGAGCAGCC CACCAGTTCC ATAACCATTG AAATGGCCGA
0481 TCAACGGACA ACCACGACCA C |
| in silico PCR Fragment |
0001 CACGGTGCCA GTGTAAGCTA TAAGGCGATC GAAGCGGCGA CTCTCGAAGA ATTGCCAGCA
0061 ACATCCGTGG CAACAGCAAC ATCCACGGCT GCAACATCAC GCGCCACTGG AGGAGCAGCA
0121 GCAACTTGTG TGGACGTTGA GTTCATACCC ACGCTAAATA TCATATTTGA GAA-GCCGAC
0181 TTCGTCGAGT GAGCAACTAC AGTGCGCCGA GAAATCGGAA CATGTGGCCG GTGGTGTGTC
0241 CGATAAGGAA CCAGCAGATG ATTGTGACTC CGACAATGAT TCTACGCGCA CATATATAAT
0301 CTCGCGAAGC AGTTGGACGG GGGTAACATC GAGTAGCAGC ACCCCATATG CGGTTACATC
0361 CACGGATACC GCCGAATTAC TCAGGCGCCA GAACAATCTC AGCCTTACGG AGGAAGATCA
0421 ATCTGTGTCG TCGTTTAGCA TCGAGCAGCC CACCAGTTCC ATAACCATTG AAATGGCCGA
0481 TCAACGGACA ACCACGACCA C |
| Assemble Data |
<PRE>
13459R-2.IR_full 1 CACGGTGCCAGTGTAAGCTATAAGGCGATCGAAGCGGCGACTCTCGAAGAATTGCCAGCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13459R-2.in silico 1 CACGGTGCCAGTGTAAGCTATAAGGCGATCGAAGCGGCGACTCTCGAAGAATTGCCAGCA 60
13459R-2.IR_full 61 ACATCCGTGGCAACAGCAACATCCACGGCTGCAACATCACGCGCCACTGGAGGAGCAGCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13459R-2.in silico 61 ACATCCGTGGCAACAGCAACATCCACGGCTGCAACATCACGCGCCACTGGAGGAGCAGCA 120
13459R-2.IR_full 121 GCAACTTGTGTGGACGTTGAGTTCATACCCACGCTAAATATCATATTTGAGAA<span class="snp"><tt>N</tt></span>GCCGAC 180
|||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||
13459R-2.in silico 121 GCAACTTGTGTGGACGTTGAGTTCATACCCACGCTAAATATCATATTTGAGAA<span class="snp"><tt>-</tt></span>GCCGAC 180
13459R-2.IR_full 181 TTCGTC<span class="snp"><tt>T</tt></span>AGTGAGCAACTACAGTGCGCCGAGAAATCGGAACATGTGGCCGGTGGTGT<span class="snp"><tt>T</tt></span>T<span class="snp"><tt>T</tt></span> 240
||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>
13459R-2.in silico 181 TTCGTC<span class="snp"><tt>G</tt></span>AGTGAGCAACTACAGTGCGCCGAGAAATCGGAACATGTGGCCGGTGGTGT<span class="snp"><tt>G</tt></span>T<span class="snp"><tt>C</tt></span> 240
13459R-2.IR_full 241 CGATAAGGAACCAGCAGATGATTGTGACTCCGACAATGATTC<span class="snp"><tt>C</tt></span>ACGCGCACATA<span class="snp"><tt>C</tt></span>ATAAT 300
||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||<span class="snp"><tt> </tt></span>|||||
13459R-2.in silico 241 CGATAAGGAACCAGCAGATGATTGTGACTCCGACAATGATTC<span class="snp"><tt>T</tt></span>ACGCGCACATA<span class="snp"><tt>T</tt></span>ATAAT 300
13459R-2.IR_full 301 CTCGCGAAGCAGTTGGACGGGGGTAACATC<span class="snp"><tt>A</tt></span>AGTAGCAGCACCCCATATGCGGTTACATC 360
||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||
13459R-2.in silico 301 CTCGCGAAGCAGTTGGACGGGGGTAACATC<span class="snp"><tt>G</tt></span>AGTAGCAGCACCCCATATGCGGTTACATC 360
13459R-2.IR_full 361 CACGGATACCGCCGAATTACTCAGGCGCCAGAACAATCTCAGCCTTACGGAGGA<span class="snp"><tt>G</tt></span>GATCA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||
13459R-2.in silico 361 CACGGATACCGCCGAATTACTCAGGCGCCAGAACAATCTCAGCCTTACGGAGGA<span class="snp"><tt>A</tt></span>GATCA 420
13459R-2.IR_full 421 ATC<span class="snp"><tt>G</tt></span>GTGTCGTCGTTTAGCATCGAGCAGCCCACCAGTTCCATAACCATTGAAATGGCCGA 480
|||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13459R-2.in silico 421 ATC<span class="snp"><tt>T</tt></span>GTGTCGTCGTTTAGCATCGAGCAGCCCACCAGTTCCATAACCATTGAAATGGCCGA 480
13459R-2.IR_full 481 TCAACGGACAACCACGACCAC 501
|||||||||||||||||||||
13459R-2.in silico 481 TCAACGGACAACCACGACCAC 501
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
1 |
4 |
NM_001032402.1 |
CG33957-RB, transcript variant B (cp309), mRNA |
| 0.2 |
1 |
3 |
3 |
7 |
NM_079729.2 |
CG4792-RA (Dcr-1), mRNA |
| 0 |
0 |
3 |
5 |
14 |
NM_176591.1 |
CG15532-RC, transcript variant C (hdc), mRNA |
| 0 |
0 |
3 |
5 |
14 |
NM_079853.2 |
CG15532-RA, transcript variant A (hdc), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_079985.2 |
CG5206-RA (bon), mRNA |
| 0 |
0 |
2 |
15 |
11 |
NM_001043262.1 |
CG34157-RE, transcript variant E (Dys), mRNA |
| 0 |
0 |
2 |
10 |
18 |
NM_135715.2 |
CG31761-RA, transcript variant A (bru-2), mRNA |
| 0 |
0 |
2 |
10 |
18 |
NM_165007.1 |
CG31761-RC, transcript variant C (bru-2), mRNA |
| 0 |
0 |
2 |
10 |
16 |
NM_176025.1 |
CG31761-RD, transcript variant D (bru-2), mRNA |
| 0 |
0 |
2 |
10 |
16 |
NM_001042891.1 |
CG31761-RE, transcript variant E (bru-2), mRNA |
| 0 |
0 |
2 |
6 |
9 |
NM_142755.1 |
CG5732-RA (CG5732), mRNA |
| 0 |
0 |
2 |
5 |
8 |
NM_144022.2 |
CG32428-RA (CG32428), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_132665.1 |
CG15753-RA (CG15753), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_168671.2 |
CG32158-RA, transcript variant A (CG32158), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_206386.1 |
CG32158-RF, transcript variant F (CG32158), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_167037.1 |
CG4165-RD, transcript variant D (CG4165), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_131992.2 |
CG4165-RA, transcript variant A (CG4165), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_167036.1 |
CG4165-RC, transcript variant C (CG4165), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_168670.1 |
CG32158-RB, transcript variant B (CG32158), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_167035.1 |
CG4165-RB, transcript variant B (CG4165), mRNA |
| 0 |
0 |
2 |
3 |
9 |
NM_169389.2 |
CG5270-RB, transcript variant B (CG5270), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_164439.2 |
CG31929-RA (Gr22c), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_138167.1 |
CG17143-RB, transcript variant B (thoc7), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_167804.1 |
CG17143-RA, transcript variant A (thoc7), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_001043241.1 |
CG14713-RB (CG14713), mRNA |
| 0 |
0 |
2 |
2 |
7 |
NM_141814.2 |
CG5270-RA, transcript variant A (CG5270), mRNA |
| 0 |
0 |
1 |
6 |
13 |
NM_142521.1 |
CG6026-RA (CG6026), mRNA |
| 0 |
0 |
1 |
6 |
12 |
NM_134763.1 |
CG14351-RA (CG14351), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_141100.1 |
CG14567-RA (CG14567), mRNA |
| 0 |
0 |
1 |
4 |
13 |
NM_001032249.1 |
CG30084-RF, transcript variant F (CG30084), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
|
|
|
