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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 13379R-4 
 Symbol CG13379  Full Name CG13379 
 CG No CG13379  Old CG No CG13379 
 Synonyms CG13379 
 Accession No (Link to NCBI) NM_140793.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgCAGCCAACATGCCGACGACAA 
 Primer Seq. 3'
aatctagaggtaccGTCCACGTCATCCTCATCATC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 CAGCCAACAT GCCGACGACA ACAGGAGCTC AGGGATCGGG AAACCAAGTT CCCACAACTA 
0061 GCACTACGAT TGTAAACCAC TTCCGGGAGT TGATCAAGGA ACCGAAGAAC CTCGACGAGG 
0121 CGGCCAACTA TCTGTACCAG TCGCTGCTCG ACGACGCCGT AGTCGGAATC TTTAACGAGA 
0181 CACATCATCT GCGAAAGTCA GGGAATCTGG CCGCCCTGGA CGGAGTGCCG GAGGACTCAA 
0241 CCTATCGAAT GTGCGAGATG CCCAACCTGG ACATATTCGG CATCTCAACG GCCAAAAAGC 
0301 CAATGGACTG CACCTGCCCC AACTGTGATC GCCTGGTGGC CGCCGCCCGC TTCGCCCCGC 
0361 ACCTGGAAAA GTGCATGGGC ATGGGTCGTA TCTCGTCGCG AATTGCCTCT CGTCGCTTGG 
0421 CCACCAAAGA GGGCGCCACA TCCGCCCACC TGCACTCTTC GGGAAATACC GGAGGCACTG 
0481 ATGATGAGGA TGACGTGGAC  
 in silico PCR Fragment
0001 CAGCCAACAT GCCGACGACA ACAGGAGCTC AGGGATCGGG AAACCAAGTT CCCACAACTA 
0061 GCACTACGAT TGTAAACCAC TTCCGGGAGT TGATCAAGGA ACCGAAGAAC CTCGACGAGG 
0121 CGGCCAACTA TCTGTACCAG TCGCTGCTCG ACGACGCCGT AGTCGGAATC TTTAACGAGA 
0181 CACATCATCT GCGAAAGTCA GGGAATCTGG CCGCCCTGGA CGGAGTGCCG GAGGACTCAA 
0241 CCTATCGAAT GTGCGAGATG CCCAACCTGG ACATATTCGG CATCTCAACG GCCAAAAAGC 
0301 CAATGGACTG CACCTGCCCC AACTGTGATC GCCTGGTGGC CGCCGCCCGC TTCGCCCCGC 
0361 ACCTGGAAAA GTGCATGGGC ATGGGTCGTA TCTCGTCGCG AATTGCCTCT CGTCGCTTGG 
0421 CCACCAAAGA GGGCGCCACA TCCGCCCACC TGCACTCTTC GGGAAATACC GGAGGCACTG 
0481 ATGATGAGGA TGACGTGGAC  
 Assemble Data

13379R-4.IR_full       1   CAGCCAACATGCCGACGACAACAGGAGCTCAGGGATCGGGAAACCAAGTTCCCACAACTA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13379R-4.in silico     1   CAGCCAACATGCCGACGACAACAGGAGCTCAGGGATCGGGAAACCAAGTTCCCACAACTA 60


13379R-4.IR_full       61  GCACTACGATTGTAAACCACTTCCGGGAGTTGATCAAGGAACCGAAGAACCTCGACGAGG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13379R-4.in silico     61  GCACTACGATTGTAAACCACTTCCGGGAGTTGATCAAGGAACCGAAGAACCTCGACGAGG 120


13379R-4.IR_full       121 CGGCCAACTATCTGTACCAGTCGCTGCTCGACGACGCCGTAGTCGGAATCTTTAACGAGA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13379R-4.in silico     121 CGGCCAACTATCTGTACCAGTCGCTGCTCGACGACGCCGTAGTCGGAATCTTTAACGAGA 180


13379R-4.IR_full       181 CACATCATCTGCGAAAGTCAGGGAATCTGGCCGCCCTGGACGGAGTGCCGGAGGACTCAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13379R-4.in silico     181 CACATCATCTGCGAAAGTCAGGGAATCTGGCCGCCCTGGACGGAGTGCCGGAGGACTCAA 240


13379R-4.IR_full       241 CCTATCGAATGTGCGAGATGCCCAACCTGGACATATTCGGCATCTCAACGGCCAAAAAGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13379R-4.in silico     241 CCTATCGAATGTGCGAGATGCCCAACCTGGACATATTCGGCATCTCAACGGCCAAAAAGC 300


13379R-4.IR_full       301 CAATGGACTGCACCTGCCCCAACTGTGATCGCCTGGTGGCCGCCGCCCGCTTCGCCCCGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13379R-4.in silico     301 CAATGGACTGCACCTGCCCCAACTGTGATCGCCTGGTGGCCGCCGCCCGCTTCGCCCCGC 360


13379R-4.IR_full       361 ACCTGGAAAAGTGCATGGGCATGGGTCGTATCTCGTCGCGAATTGCCTCTCGTCGCTTGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13379R-4.in silico     361 ACCTGGAAAAGTGCATGGGCATGGGTCGTATCTCGTCGCGAATTGCCTCTCGTCGCTTGG 420


13379R-4.IR_full       421 CCACCAAAGAGGGCGCCACATCCGCCCACCTGCACTCTTCGGGAAATACCGGAGGCACTG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13379R-4.in silico     421 CCACCAAAGAGGGCGCCACATCCGCCCACCTGCACTCTTCGGGAAATACCGGAGGCACTG 480


13379R-4.IR_full       481 ATGATGAGGATGACGTGGAC 500
                           ||||||||||||||||||||
13379R-4.in silico     481 ATGATGAGGATGACGTGGAC 500

 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_140793.1  CG13379-RA (CG13379), mRNA 
0   NM_169826.1  CG31224-RA (CG31224), mRNA 
0   NM_138012.2  CG2980-RA (thoc5), mRNA 
0   NM_078831.1  CG5006-RA (Or33c), mRNA 
0   NM_165123.2  CG3938-RC, transcript variant C (CycE), mRNA 
0   NM_057611.4  CG3938-RA, transcript variant A (CycE), mRNA 
0   NM_165125.2  CG3938-RE, transcript variant E (CycE), mRNA 
0   NM_165124.2  CG3938-RD, transcript variant D (CycE), mRNA 
0   NM_057612.4  CG3938-RB, transcript variant B (CycE), mRNA 
0   NM_176735.1  CG33206-RB, transcript variant B (l(1)G0168), mRNA 
0   NM_176734.1  CG33206-RA, transcript variant A (l(1)G0168), mRNA 
0   NM_167647.2  CG12199-RB, transcript variant B (kek5), mRNA 
0   NM_133154.2  CG12199-RA, transcript variant A (kek5), mRNA 
0   NM_140401.1  CG10741-RB, transcript variant B (CG10741), mRNA 
0   NM_168555.1  CG10741-RA, transcript variant A (CG10741), mRNA 
0   NM_169806.1  CG31235-RA (CG31235), mRNA 
0   NM_168670.1  CG32158-RB, transcript variant B (CG32158), mRNA 
0   NM_140919.1  CG7385-RA (CG7385), mRNA 
0   NM_058154.3  CG10060-RA (G-ialpha65A), mRNA 
0   NM_057685.3  CG11527-RA (Tig), mRNA 
0   NM_142495.1  CG14299-RA, transcript variant A (CG14299), mRNA 
0   NM_164689.1  CG11567-RB, transcript variant B (Cpr), mRNA 
0   NM_141538.1  CG7352-RA (CG7352), mRNA 
0   NM_176184.2  CG18250-RC, transcript variant C (Dg), mRNA 
0   NM_136243.2  CG9257-RA (CG9257), mRNA 
0   NM_133049.1  CG5988-RA (upd2), mRNA 
0   10  NM_142975.2  CG5669-RA (CG5669), mRNA 
0   NM_057737.2  CG15804-RA, transcript variant A (Dhc62B), mRNA 
0   NM_206236.1  CG15804-RB, transcript variant B (Dhc62B), mRNA 
0   NM_142220.1  CG6045-RA (CG6045), mRNA 
 Wing Disc
GFP
Puc
Caspase
DAPI
Merge
Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
DB
 
DC
DM
VM
 
VB
 
Dcompartment   Penetrance
     

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