NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

13289R-1

Symbol

CG33993

Full Name

CG33993

CG No

CG33993

Old CG No

CG13289

Synonyms

CG13289, CG13290, CG33993

Accession No (Link to NCBI)

NM_001038902.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCATTGGACTGCTGCGGGGAA

Primer Seq. 3'

aatctagaggtaccTGGTGAAGGTGGTGGCACTCG

Predicted Fragment Size

IR fragment full Seq

0001 GCATTGGACT GCTGCGGGGA ACCGAGGACA GCCGCCCCCT GATGCACAGC ACCTGCGGCT 
0061 CCTCGCTGAC CTCCGGATCC GGCTGTGGAT CCACTCAGAC CATCTCCGAT GGCTATGTGA 
0121 ACGAGGCCAT CTGCGAGCCG GACAAGTACG TGGCCAGCAA GTTCGGCGAC TCCTGCCGCC 
0181 AGTCACTGTC CGCACTGGAG AGTGCCACCC AGCGGCTGCA GGTGGAGCTG CCCGCCGCCA 
0241 GCAAAAAGTA TCTGAGAGAG ACGTGCAGCG CCAACTCCAG TCCGAAATTG TTTCCCGGCC 
0301 ACAGTGCCCT GCGCCTGGAT AATCTCAGCC TATCCGAGCA GCAGGAGCTT AAGGGAGCCG 
0361 CCTGGTTTCA AGCGGGCATT CCCAGGGAAA TATCCCTGGA GGTCCTGTCC CGACAGAGTC 
0421 CAGGTGCGTT TCTAGTGCGC CAGAGCAGCA CAAAGCCGGG ATGTTTCGCC CTCTCGCTGC 
0481 GAGTGCCACC ACCTTCACCA

in silico PCR Fragment

0001 GCATTGGACT GCTGCGGGGA ACCGAGGACA GCCGCCCCCT GATGCACAGC ACCTGCGGCT 
0061 CCTCGCTGAC CTCCGGATCC GGCTGTGGAT CCACTCAGAC CATCTCCGAT GGCTATGTGA 
0121 ACGAGGCCAT CTGCGAGCCG GACAAGTACG TGGCCAGCAA GTTCGGCGAC TCCTGCCGCC 
0181 AGTCACTGTC CGCACTGGAG AGTGCCACCC AGCGGCTGCA GGTGGAGCTG CCCGCCGCCA 
0241 GCAAAAAGTA TCTGAGAGAG ACGTGCAGCG CCAACTCCAG TCCGAAATTG TTTCCCGGCC 
0301 ACAGTGCCCT GCGCCTGGAT AATCTCAGCC TAGCCGAGCA GCAGGAGCTT AAGGGAGCCG 
0361 CCTGGTTTCA AGCGGGCATT CCCAGGGAAA TATCCCTGGA GGCCCTGTCC CGACAGAGTC 
0421 CAGGTGCGTT TCTAGTGCGC CAGAGCAGCA CAAAGCCGGG ATGTTTCGCC CTCTCGCTGC 
0481 GAGTGCCACC ACCTTCACCA

Assemble Data

<PRE>

13289R-1.IR_full       1   GCATTGGACTGCTGCGGGGAACCGAGGACAGCCGCCCCCTGATGCACAGCACCTGCGGCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13289R-1.in silico     1   GCATTGGACTGCTGCGGGGAACCGAGGACAGCCGCCCCCTGATGCACAGCACCTGCGGCT 60


13289R-1.IR_full       61  CCTCGCTGACCTCCGGATCCGGCTGTGGATCCACTCAGACCATCTCCGATGGCTATGTGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13289R-1.in silico     61  CCTCGCTGACCTCCGGATCCGGCTGTGGATCCACTCAGACCATCTCCGATGGCTATGTGA 120


13289R-1.IR_full       121 ACGAGGCCATCTGCGAGCCGGACAAGTACGTGGCCAGCAAGTTCGGCGACTCCTGCCGCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13289R-1.in silico     121 ACGAGGCCATCTGCGAGCCGGACAAGTACGTGGCCAGCAAGTTCGGCGACTCCTGCCGCC 180


13289R-1.IR_full       181 AGTCACTGTCCGCACTGGAGAGTGCCACCCAGCGGCTGCAGGTGGAGCTGCCCGCCGCCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13289R-1.in silico     181 AGTCACTGTCCGCACTGGAGAGTGCCACCCAGCGGCTGCAGGTGGAGCTGCCCGCCGCCA 240


13289R-1.IR_full       241 GCAAAAAGTATCTGAGAGAGACGTGCAGCGCCAACTCCAGTCCGAAATTGTTTCCCGGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13289R-1.in silico     241 GCAAAAAGTATCTGAGAGAGACGTGCAGCGCCAACTCCAGTCCGAAATTGTTTCCCGGCC 300


13289R-1.IR_full       301 ACAGTGCCCTGCGCCTGGATAATCTCAGCCTA<span class="snp"><tt>T</tt></span>CCGAGCAGCAGGAGCTTAAGGGAGCCG 360
                           ||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||
13289R-1.in silico     301 ACAGTGCCCTGCGCCTGGATAATCTCAGCCTA<span class="snp"><tt>G</tt></span>CCGAGCAGCAGGAGCTTAAGGGAGCCG 360


13289R-1.IR_full       361 CCTGGTTTCAAGCGGGCATTCCCAGGGAAATATCCCTGGAGG<span class="snp"><tt>T</tt></span>CCTGTCCCGACAGAGTC 420
                           ||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||
13289R-1.in silico     361 CCTGGTTTCAAGCGGGCATTCCCAGGGAAATATCCCTGGAGG<span class="snp"><tt>C</tt></span>CCTGTCCCGACAGAGTC 420


13289R-1.IR_full       421 CAGGTGCGTTTCTAGTGCGCCAGAGCAGCACAAAGCCGGGATGTTTCGCCCTCTCGCTGC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13289R-1.in silico     421 CAGGTGCGTTTCTAGTGCGCCAGAGCAGCACAAAGCCGGGATGTTTCGCCCTCTCGCTGC 480


13289R-1.IR_full       481 GAGTGCCACCACCTTCACCA 500
                           ||||||||||||||||||||
13289R-1.in silico     481 GAGTGCCACCACCTTCACCA 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_001038902.1	CG33993-RA (CG33993), mRNA
0.2	1	2	2	3	NM_169411.2	CG6808-RA (CG6808), mRNA
0	0	3	22	38	NM_168153.1	CG32406-RA (CG32406), mRNA
0	0	3	3	8	NM_206803.1	CG1417-RH, transcript variant H (slgA), mRNA
0	0	3	3	8	NM_078709.2	CG1417-RE, transcript variant E (slgA), mRNA
0	0	3	3	8	NM_167751.1	CG1417-RA, transcript variant A (slgA), mRNA
0	0	3	3	8	NM_167752.1	CG1417-RD, transcript variant D (slgA), mRNA
0	0	3	3	8	NM_167753.1	CG1417-RB, transcript variant B (slgA), mRNA
0	0	3	3	8	NM_206805.1	CG1417-RF, transcript variant F (slgA), mRNA
0	0	3	3	8	NM_206804.1	CG1417-RG, transcript variant G (slgA), mRNA
0	0	2	6	3	NM_139732.2	CG10542-RA (CG10542), mRNA
0	0	2	4	5	NM_137035.1	CG6061-RA (mip120), mRNA
0	0	2	3	2	NM_141674.2	CG9448-RA (CG9448), mRNA
0	0	2	2	9	NM_078696.2	CG1467-RA (Syx16), mRNA
0	0	2	2	4	NM_167626.1	CG6531-RA (wgn), mRNA
0	0	1	5	2	NM_141369.1	CG1153-RA (Osi7), mRNA
0	0	1	4	5	NM_132687.1	CG9941-RA (CG9941), mRNA
0	0	1	4	3	NM_079206.2	CG7503-RA (Con), mRNA
0	0	1	4	3	NM_164869.1	CG18660-RA, transcript variant A (Nckx30C), mRNA
0	0	1	4	3	NM_164870.1	CG18660-RB, transcript variant B (Nckx30C), mRNA
0	0	1	4	2	NM_143754.2	CG18660-RC, transcript variant C (Nckx30C), mRNA
0	0	1	3	8	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
0	0	1	3	8	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
0	0	1	3	5	NM_135105.1	CG12511-RA (CG12511), mRNA
0	0	1	3	4	NM_142635.2	CG5180-RA (CG5180), mRNA
0	0	1	3	4	NM_141089.1	CG11249-RA (CG11249), mRNA
0	0	1	3	3	NM_169761.1	CG31190-RA, transcript variant A (CG31190), mRNA
0	0	1	3	3	NM_206504.1	CG31190-RB, transcript variant B (CG31190), mRNA
0	0	1	3	2	NM_167392.1	CG32614-RA (CG32614), mRNA
0	0	1	3	2	NM_079544.3	CG2505-RA (alpha-Est2), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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