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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
13243R-4 |
| Symbol |
CG13243 |
Full Name |
CG13243 |
| CG No |
CG13243 |
Old CG No |
CG13243 |
| Synonyms |
BG:DS02252.1, CG13243 |
| Accession No (Link to NCBI) |
NM_135925.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
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| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGTGACCTGTGACATGGAGCA |
| Primer Seq. 3' |
aatctagaggtaccCTACCCGAGTATTGTCGATTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGTGACCTGT GACATGGAGC AGTTTCGCGA TCCAAACAAG AAGCGTGCCG CCAACGATGC
0061 CTGCGAGGAG CTGGACAAGC TGGATGCCCA GGACGAGATC AGTGTGTCGA GTATCATTTC
0121 GGCGCTATTG AGACCGATTG AGCCCTCGGA CGATCAGTAC AAGGTGTGCG ACTGGGGCAT
0181 CAATCCCAAG CAGTTTATGC CCACCAAACA GGCGATCATT TTCAAGGAAC AGTTGTTCAT
0241 ATCGAAGTTG CGGAATACAA ATTGCAAGCT AAATGAAAAT CTAAAAATCT TCTTTGAGCG
0301 GGAACTTGAG CAGATTGGAC GCTTCTGTTT GAATGTGGAG GAATCCTTTG ATGGCTATGT
0361 GATCTTCAGC AGCAGCAAAA TGAAGAAGGG CAAGGGTATG ACGGAATGTG GTCACCAGTT
0421 AAAGGGGAAG TACGATGACA AGTTCCTTCT CATCTGCGAG AAGAGATCGG AGTTCGACCG
0481 AATCGACAAT ACTCGGGTAG |
| in silico PCR Fragment |
0001 TGTGACCTGT GACATGGAGC AGTTTCGCGA TCCAAACAAG AAGCGTGCCG CCAACGATGC
0061 CTGCGAGGAG CTGGACAAGC TGGATGCCCA GGACGAGATC AGTGTGTCGA GTATCATTTC
0121 GGCGCTATTG AGACCGATTG AGCCCTCGGA CGATCAGTAC AAGGTGTGCG ACTGGGGCAT
0181 CAATCCCAAG CAGTTTATGC CCACCAAACA GGCGATCATT TTCAAGGAAC AGTTGTTCAT
0241 ATCGAAGTTG CGGAATACAA ATTGCAAGCT AAATGAAAAT CTAAAAATCT TCTTTGAGCG
0301 GGAACTTGAG CAGATTGGAC GCTTCTGTTT GAATGTGGAG GAATCCTTTG ATGGCTATGT
0361 GATCTTCAGC AGCAGCAAAA TGAAGAAGGG CAAGGGTATG ACGGAATGTG GTCACCAGTT
0421 AAAGGGGAAG TACGATGACA AGTTCCTTCT CATCTGCGAG AAGAGATCGG AGTTCGACCG
0481 AATCGACAAT ACTCGGGTAG |
| Assemble Data |
13243R-4.IR_full 1 TGTGACCTGTGACATGGAGCAGTTTCGCGATCCAAACAAGAAGCGTGCCGCCAACGATGC 60
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13243R-4.in silico 1 TGTGACCTGTGACATGGAGCAGTTTCGCGATCCAAACAAGAAGCGTGCCGCCAACGATGC 60
13243R-4.IR_full 61 CTGCGAGGAGCTGGACAAGCTGGATGCCCAGGACGAGATCAGTGTGTCGAGTATCATTTC 120
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13243R-4.in silico 61 CTGCGAGGAGCTGGACAAGCTGGATGCCCAGGACGAGATCAGTGTGTCGAGTATCATTTC 120
13243R-4.IR_full 121 GGCGCTATTGAGACCGATTGAGCCCTCGGACGATCAGTACAAGGTGTGCGACTGGGGCAT 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-4.in silico 121 GGCGCTATTGAGACCGATTGAGCCCTCGGACGATCAGTACAAGGTGTGCGACTGGGGCAT 180
13243R-4.IR_full 181 CAATCCCAAGCAGTTTATGCCCACCAAACAGGCGATCATTTTCAAGGAACAGTTGTTCAT 240
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13243R-4.in silico 181 CAATCCCAAGCAGTTTATGCCCACCAAACAGGCGATCATTTTCAAGGAACAGTTGTTCAT 240
13243R-4.IR_full 241 ATCGAAGTTGCGGAATACAAATTGCAAGCTAAATGAAAATCTAAAAATCTTCTTTGAGCG 300
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13243R-4.in silico 241 ATCGAAGTTGCGGAATACAAATTGCAAGCTAAATGAAAATCTAAAAATCTTCTTTGAGCG 300
13243R-4.IR_full 301 GGAACTTGAGCAGATTGGACGCTTCTGTTTGAATGTGGAGGAATCCTTTGATGGCTATGT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-4.in silico 301 GGAACTTGAGCAGATTGGACGCTTCTGTTTGAATGTGGAGGAATCCTTTGATGGCTATGT 360
13243R-4.IR_full 361 GATCTTCAGCAGCAGCAAAATGAAGAAGGGCAAGGGTATGACGGAATGTGGTCACCAGTT 420
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13243R-4.in silico 361 GATCTTCAGCAGCAGCAAAATGAAGAAGGGCAAGGGTATGACGGAATGTGGTCACCAGTT 420
13243R-4.IR_full 421 AAAGGGGAAGTACGATGACAAGTTCCTTCTCATCTGCGAGAAGAGATCGGAGTTCGACCG 480
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13243R-4.in silico 421 AAAGGGGAAGTACGATGACAAGTTCCTTCTCATCTGCGAGAAGAGATCGGAGTTCGACCG 480
13243R-4.IR_full 481 AATCGACAATACTCGGGTAG 500
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13243R-4.in silico 481 AATCGACAATACTCGGGTAG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_135925.2 |
CG13243-RA (CG13243), mRNA |
| 0.2 |
1 |
4 |
3 |
3 |
NM_167637.1 |
CG32544-RA (CG32544), mRNA |
| 0 |
0 |
5 |
10 |
9 |
NM_170379.2 |
CG31048-RA (CG31048), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_165479.1 |
CG15845-RA, transcript variant A (Adf1), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_206028.1 |
CG15845-RC, transcript variant C (Adf1), mRNA |
| 0 |
0 |
4 |
3 |
2 |
NM_165480.1 |
CG15845-RB, transcript variant B (Adf1), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_139632.1 |
CG1319-RA (CG1319), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_138047.2 |
CG4065-RA, transcript variant A (CG4065), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_001014546.1 |
CG4065-RB, transcript variant B (CG4065), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_143492.2 |
CG15522-RA (CG15522), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_140291.1 |
CG17152-RA (CG17152), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_132375.1 |
CG2972-RA (CG2972), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_135005.1 |
CG11929-RA (CG11929), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001031870.1 |
CG15892-RA, transcript variant A (CG15892), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_001031869.1 |
CG15892-RB, transcript variant B (CG15892), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167069.1 |
CG15891-RA, transcript variant A (CG15891), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_206435.1 |
CG33324-RA (CG33324), mRNA |
| 0 |
0 |
1 |
5 |
14 |
NM_079919.2 |
CG5700-RB (prc), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_132009.2 |
CG11473-RA (CG11473), mRNA |
| 0 |
0 |
1 |
4 |
6 |
NM_001038909.1 |
CG33205-RI, transcript variant I (CG33205), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_139819.2 |
CG8638-RA (CG8638), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_166874.1 |
CG3638-RA, transcript variant A (CG3638), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_130541.3 |
CG3638-RC, transcript variant C (CG3638), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_166872.1 |
CG3638-RD, transcript variant D (CG3638), mRNA |
| 0 |
0 |
1 |
3 |
8 |
NM_166873.1 |
CG3638-RB, transcript variant B (CG3638), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_078866.2 |
CG6549-RA, transcript variant A (fws), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_165221.1 |
CG6549-RC, transcript variant C (fws), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_057262.2 |
CG1828-RA, transcript variant A (dre4), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_167922.1 |
CG1828-RB, transcript variant B (dre4), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136727.2 |
CG18408-RA, transcript variant A (CAP), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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