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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

13243R-1

Symbol

CG13243

Full Name

CG13243

CG No

CG13243

Old CG No

CG13243

Synonyms

BG:DS02252.1, CG13243

Accession No (Link to NCBI)

NM_135925.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGTGACCTGTGACATGGAGCA

Primer Seq. 3'

aatctagaggtaccCTACCCGAGTATTGTCGATTC

Predicted Fragment Size

IR fragment full Seq

0001 TGTGACCTGT GACATGGAGC AGTTTCGCGA TCCAAACAAG AAGCGTGCCG CCAACGATGC 
0061 CTGCGAGGAG CTGGACAAGC TGGATGCCCA GGACGAGATC AGTGTGTCGA GTATCATTTC 
0121 GGCGCTATTG AGACCGATTG AGCCCTCGGA CGATCAGTAC AAGGTGTGCG ACTGGGGCAT 
0181 CAATCCCAAG CAGTTTATGC CCACCAAACA GGCGATCATT TTCAAGGAAC AGTTGTTCAT 
0241 ATCGAAGTTG CGGAATACAA ATTGCAAGCT AAATGAAAAT CTAAAAATCT TCTTTGAGCG 
0301 GGAACTTGAG CAGATTGGAC GCTTCTGTTT GAATGTGGAG GAATCCTTTG ATGGCTATGT 
0361 GATCTTCAGC AGCAGCAAAA TGAAGAAGGG CAAGGGTATG ACGGAATGTG GTCACCAGTT 
0421 AAAGGGGAAG TACGATGACA AGTTCCTTCT CATCTGCGAG AAGAGATCGG AGTTCGACCG 
0481 AATCGACAAT ACTCGGGTAG

in silico PCR Fragment

0001 TGTGACCTGT GACATGGAGC AGTTTCGCGA TCCAAACAAG AAGCGTGCCG CCAACGATGC 
0061 CTGCGAGGAG CTGGACAAGC TGGATGCCCA GGACGAGATC AGTGTGTCGA GTATCATTTC 
0121 GGCGCTATTG AGACCGATTG AGCCCTCGGA CGATCAGTAC AAGGTGTGCG ACTGGGGCAT 
0181 CAATCCCAAG CAGTTTATGC CCACCAAACA GGCGATCATT TTCAAGGAAC AGTTGTTCAT 
0241 ATCGAAGTTG CGGAATACAA ATTGCAAGCT AAATGAAAAT CTAAAAATCT TCTTTGAGCG 
0301 GGAACTTGAG CAGATTGGAC GCTTCTGTTT GAATGTGGAG GAATCCTTTG ATGGCTATGT 
0361 GATCTTCAGC AGCAGCAAAA TGAAGAAGGG CAAGGGTATG ACGGAATGTG GTCACCAGTT 
0421 AAAGGGGAAG TACGATGACA AGTTCCTTCT CATCTGCGAG AAGAGATCGG AGTTCGACCG 
0481 AATCGACAAT ACTCGGGTAG

Assemble Data


13243R-1.IR_full       1   TGTGACCTGTGACATGGAGCAGTTTCGCGATCCAAACAAGAAGCGTGCCGCCAACGATGC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-1.in silico     1   TGTGACCTGTGACATGGAGCAGTTTCGCGATCCAAACAAGAAGCGTGCCGCCAACGATGC 60


13243R-1.IR_full       61  CTGCGAGGAGCTGGACAAGCTGGATGCCCAGGACGAGATCAGTGTGTCGAGTATCATTTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-1.in silico     61  CTGCGAGGAGCTGGACAAGCTGGATGCCCAGGACGAGATCAGTGTGTCGAGTATCATTTC 120


13243R-1.IR_full       121 GGCGCTATTGAGACCGATTGAGCCCTCGGACGATCAGTACAAGGTGTGCGACTGGGGCAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-1.in silico     121 GGCGCTATTGAGACCGATTGAGCCCTCGGACGATCAGTACAAGGTGTGCGACTGGGGCAT 180


13243R-1.IR_full       181 CAATCCCAAGCAGTTTATGCCCACCAAACAGGCGATCATTTTCAAGGAACAGTTGTTCAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-1.in silico     181 CAATCCCAAGCAGTTTATGCCCACCAAACAGGCGATCATTTTCAAGGAACAGTTGTTCAT 240


13243R-1.IR_full       241 ATCGAAGTTGCGGAATACAAATTGCAAGCTAAATGAAAATCTAAAAATCTTCTTTGAGCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-1.in silico     241 ATCGAAGTTGCGGAATACAAATTGCAAGCTAAATGAAAATCTAAAAATCTTCTTTGAGCG 300


13243R-1.IR_full       301 GGAACTTGAGCAGATTGGACGCTTCTGTTTGAATGTGGAGGAATCCTTTGATGGCTATGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-1.in silico     301 GGAACTTGAGCAGATTGGACGCTTCTGTTTGAATGTGGAGGAATCCTTTGATGGCTATGT 360


13243R-1.IR_full       361 GATCTTCAGCAGCAGCAAAATGAAGAAGGGCAAGGGTATGACGGAATGTGGTCACCAGTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-1.in silico     361 GATCTTCAGCAGCAGCAAAATGAAGAAGGGCAAGGGTATGACGGAATGTGGTCACCAGTT 420


13243R-1.IR_full       421 AAAGGGGAAGTACGATGACAAGTTCCTTCTCATCTGCGAGAAGAGATCGGAGTTCGACCG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13243R-1.in silico     421 AAAGGGGAAGTACGATGACAAGTTCCTTCTCATCTGCGAGAAGAGATCGGAGTTCGACCG 480


13243R-1.IR_full       481 AATCGACAATACTCGGGTAG 500
                           ||||||||||||||||||||
13243R-1.in silico     481 AATCGACAATACTCGGGTAG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135925.2	CG13243-RA (CG13243), mRNA
0.2	1	4	3	3	NM_167637.1	CG32544-RA (CG32544), mRNA
0	0	5	10	9	NM_170379.2	CG31048-RA (CG31048), mRNA
0	0	4	3	2	NM_165479.1	CG15845-RA, transcript variant A (Adf1), mRNA
0	0	4	3	2	NM_206028.1	CG15845-RC, transcript variant C (Adf1), mRNA
0	0	4	3	2	NM_165480.1	CG15845-RB, transcript variant B (Adf1), mRNA
0	0	3	2	2	NM_139632.1	CG1319-RA (CG1319), mRNA
0	0	2	4	5	NM_138047.2	CG4065-RA, transcript variant A (CG4065), mRNA
0	0	2	4	5	NM_001014546.1	CG4065-RB, transcript variant B (CG4065), mRNA
0	0	2	3	5	NM_143492.2	CG15522-RA (CG15522), mRNA
0	0	2	3	4	NM_140291.1	CG17152-RA (CG17152), mRNA
0	0	2	2	3	NM_132375.1	CG2972-RA (CG2972), mRNA
0	0	2	2	2	NM_135005.1	CG11929-RA (CG11929), mRNA
0	0	2	2	2	NM_001031870.1	CG15892-RA, transcript variant A (CG15892), mRNA
0	0	2	2	2	NM_001031869.1	CG15892-RB, transcript variant B (CG15892), mRNA
0	0	2	2	2	NM_167069.1	CG15891-RA, transcript variant A (CG15891), mRNA
0	0	1	6	3	NM_206435.1	CG33324-RA (CG33324), mRNA
0	0	1	5	14	NM_079919.2	CG5700-RB (prc), mRNA
0	0	1	5	2	NM_132009.2	CG11473-RA (CG11473), mRNA
0	0	1	4	6	NM_001038909.1	CG33205-RI, transcript variant I (CG33205), mRNA
0	0	1	3	9	NM_139819.2	CG8638-RA (CG8638), mRNA
0	0	1	3	8	NM_166874.1	CG3638-RA, transcript variant A (CG3638), mRNA
0	0	1	3	8	NM_130541.3	CG3638-RC, transcript variant C (CG3638), mRNA
0	0	1	3	8	NM_166872.1	CG3638-RD, transcript variant D (CG3638), mRNA
0	0	1	3	8	NM_166873.1	CG3638-RB, transcript variant B (CG3638), mRNA
0	0	1	3	6	NM_078866.2	CG6549-RA, transcript variant A (fws), mRNA
0	0	1	3	6	NM_165221.1	CG6549-RC, transcript variant C (fws), mRNA
0	0	1	3	5	NM_057262.2	CG1828-RA, transcript variant A (dre4), mRNA
0	0	1	3	5	NM_167922.1	CG1828-RB, transcript variant B (dre4), mRNA
0	0	1	3	4	NM_136727.2	CG18408-RA, transcript variant A (CAP), mRNA

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