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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 13160R-4

Symbol CG13160 Full Name CG13160

CG No CG13160 Old CG No CG13160

Synonyms CG13160

Accession No (Link to NCBI) NM_165890.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTCGGGGTCTGAGAACCTAAT

Primer Seq. 3'

aatctagaggtaccAACCCGGCTTGGTATCGTAGT

Predicted Fragment Size

IR fragment full Seq

0001 TTCGGGGTCT GAGAACCTAA TAATCGAGGT GGATGAGGAC AAGATCCGTT GCAAGCAGTA 
0061 TTCCTGGTAC TTTGCCCCCA CCTACCTACT GTTCTGGGTG GGTCTCTTCT TCGCCGTCAT 
0121 CTATCCACTT TTCCAGGCCT TGCCCACCGG AATCAAAATC TCCGAGGAGG CGGATAAACC 
0181 GGGACAATTC GTGGCCGAGA GAGCTCAGGA AATACTGCTG AAAATAAGTC GATTGGGACC 
0241 CCGTGTGGTG GGTGATGTTG ATAATGAAGT CACAGTTGTG AATTTGCTGC TCGCTGAAAT 
0301 CGAAAAAGTG CGCCAGGTGC TGCGAGACGA TGTCTATGAA ATGGAGGTGG AGGTGCAACG 
0361 GGCATCCGGA TCCTACTTGA TCAAAGGCCT AACGAATCAC TATCAGGGTG TGCAGAATGT 
0421 GATCGTTAGA CTGAGTACGA AGAGCTCAAA TAGCACCTCC TACCTTTTGG TCAATAGTCA 
0481 CTACGATACC AAGCCGGGTT

in silico PCR Fragment

0001 TTCGGGGTCT GAGAACCTAA TAATCGAGGT GGATGAGGAC AAGATCCGTT GCAAGCAGTA 
0061 TTCCTGGTAC TTTGCCCCCA TCTACCTCCT GTTCTGGGTG GGTCTCTTCT TCGCCGTCAT 
0121 ATATCCACTT TTCCAGGCCT TGCCCACCGG AATCAAAATC TCCGAGGAGG CGGATAAACC 
0181 GGGACAATTC GTGGCCGAGA GAGCTCAGGA AATACTGCTG CAAATAAGTC GATTGGGACC 
0241 CCGTGTGGTG GGTGATGTTG ATAATGAAGT CACAGTTGTG AATTTGCTGC TCGCTGAAAT 
0301 CGAAAAAGTG CGCCAGGTGC TGCGAGACGA TGTCTATGAA ATGGAGGTGG AGGTGCAACG 
0361 GGCATCCGGA TCCTACTTGA TCAAAGGCCT AACGAATCAC TATCAGGGTG TGCAGAATGT 
0421 GATCGTTAGA CTGAGTACGA AGAGCTCAAA TAGCACCTCC TACCTTTTGG TCAATAGTCA 
0481 CTACGATACC AAGCCGGGTT

Assemble Data


13160R-4.IR_full       1   TTCGGGGTCTGAGAACCTAATAATCGAGGTGGATGAGGACAAGATCCGTTGCAAGCAGTA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13160R-4.in silico     1   TTCGGGGTCTGAGAACCTAATAATCGAGGTGGATGAGGACAAGATCCGTTGCAAGCAGTA 60


13160R-4.IR_full       61  TTCCTGGTACTTTGCCCCCACCTACCTACTGTTCTGGGTGGGTCTCTTCTTCGCCGTCAT 120
                           |||||||||||||||||||| |||||| ||||||||||||||||||||||||||||||||
13160R-4.in silico     61  TTCCTGGTACTTTGCCCCCATCTACCTCCTGTTCTGGGTGGGTCTCTTCTTCGCCGTCAT 120


13160R-4.IR_full       121 CTATCCACTTTTCCAGGCCTTGCCCACCGGAATCAAAATCTCCGAGGAGGCGGATAAACC 180
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13160R-4.in silico     121 ATATCCACTTTTCCAGGCCTTGCCCACCGGAATCAAAATCTCCGAGGAGGCGGATAAACC 180


13160R-4.IR_full       181 GGGACAATTCGTGGCCGAGAGAGCTCAGGAAATACTGCTGAAAATAAGTCGATTGGGACC 240
                           |||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
13160R-4.in silico     181 GGGACAATTCGTGGCCGAGAGAGCTCAGGAAATACTGCTGCAAATAAGTCGATTGGGACC 240


13160R-4.IR_full       241 CCGTGTGGTGGGTGATGTTGATAATGAAGTCACAGTTGTGAATTTGCTGCTCGCTGAAAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13160R-4.in silico     241 CCGTGTGGTGGGTGATGTTGATAATGAAGTCACAGTTGTGAATTTGCTGCTCGCTGAAAT 300


13160R-4.IR_full       301 CGAAAAAGTGCGCCAGGTGCTGCGAGACGATGTCTATGAAATGGAGGTGGAGGTGCAACG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13160R-4.in silico     301 CGAAAAAGTGCGCCAGGTGCTGCGAGACGATGTCTATGAAATGGAGGTGGAGGTGCAACG 360


13160R-4.IR_full       361 GGCATCCGGATCCTACTTGATCAAAGGCCTAACGAATCACTATCAGGGTGTGCAGAATGT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13160R-4.in silico     361 GGCATCCGGATCCTACTTGATCAAAGGCCTAACGAATCACTATCAGGGTGTGCAGAATGT 420


13160R-4.IR_full       421 GATCGTTAGACTGAGTACGAAGAGCTCAAATAGCACCTCCTACCTTTTGGTCAATAGTCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13160R-4.in silico     421 GATCGTTAGACTGAGTACGAAGAGCTCAAATAGCACCTCCTACCTTTTGGTCAATAGTCA 480


13160R-4.IR_full       481 CTACGATACCAAGCCGGGTT 500
                           ||||||||||||||||||||
13160R-4.in silico     481 CTACGATACCAAGCCGGGTT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_165890.2	CG13160-RA (CG13160), mRNA
0.41	2	2	2	2	NM_166988.3	CG2947-RA, transcript variant A (CG2947), mRNA
0.41	2	2	2	2	NM_001014719.1	CG2947-RC, transcript variant C (CG2947), mRNA
0.41	2	2	2	2	NM_166989.3	CG2947-RB, transcript variant B (CG2947), mRNA
0.41	2	2	2	2	NM_130718.3	CG32789-RA (CG32789), mRNA
0	0	3	19	29	NM_176155.1	CG33012-RB, transcript variant B (CG33012), mRNA
0	0	3	19	29	NM_176154.1	CG33012-RA, transcript variant A (CG33012), mRNA
0	0	3	3	11	NM_137572.2	CG10062-RA (CG10062), mRNA
0	0	3	2	10	NM_206360.1	CG33261-RF, transcript variant F (Trl), mRNA
0	0	3	2	9	NM_206357.2	CG33261-RD, transcript variant D (Trl), mRNA
0	0	3	2	9	NM_001038926.1	CG33261-RI, transcript variant I (Trl), mRNA
0	0	3	2	9	NM_206356.2	CG33261-RB, transcript variant B (Trl), mRNA
0	0	3	2	9	NM_206358.2	CG33261-RA, transcript variant A (Trl), mRNA
0	0	3	2	3	NM_080051.2	CG1214-RA (ru), mRNA
0	0	2	3	5	NM_144047.1	CG18679-RA (CG18679), mRNA
0	0	2	3	2	NM_206573.1	CG31094-RB, transcript variant B (LpR1), mRNA
0	0	2	3	2	NM_170240.2	CG31094-RA, transcript variant A (LpR1), mRNA
0	0	1	5	3	NM_136126.3	CG10195-RA (CG10195), mRNA
0	0	1	3	2	NM_080057.2	CG2708-RA (Tom34), mRNA
0	0	1	2	8	NM_166342.1	CG11961-RA, transcript variant A (CG11961), mRNA
0	0	1	2	8	NM_137569.3	CG11961-RB, transcript variant B (CG11961), mRNA
0	0	1	2	7	NM_206096.1	CG13207-RD, transcript variant D (nompA), mRNA
0	0	1	2	7	NM_165822.2	CG13207-RA, transcript variant A (nompA), mRNA
0	0	1	2	7	NM_165821.2	CG13207-RC, transcript variant C (nompA), mRNA
0	0	1	2	7	NM_080092.2	CG13207-RB, transcript variant B (nompA), mRNA
0	0	1	2	3	NM_057245.2	CG6189-RA (l(1)1Bi), mRNA
0	0	0	8	23	NM_165889.1	CG30043-RA (CG30043), mRNA
0	0	0	6	3	NM_132796.2	CG11655-RA (CG11655), mRNA
0	0	0	5	21	NM_165888.2	CG30049-RA (CG30049), mRNA
0	0	0	5	13	NM_165887.1	CG30047-RA (CG30047), mRNA

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