NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

13057R-4

Symbol

CG33061

Full Name

CG33061

CG No

CG33061

Old CG No

CG13057

Synonyms

CG13057, CG33061

Accession No (Link to NCBI)

NM_080256.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCCATTGTGCTGGTCTCCATC

Primer Seq. 3'

aatctagaggtaccAGTGGTGGCTGCTGGCGAATC

Predicted Fragment Size

IR fragment full Seq

0001 GCCANTTTGT GCTGGTCTCC ATCgGAGCTT CGCATACAGC CAGCTTGGAG TGGCCCTCGA 
0061 ATCTTGTGGC TCTCAGTTCG GTCAAGTCCN TCGCAGCTGC TTCCGATTGC CAGCGAGGAT 
0121 TCCGTTGAGT TGGCCGATGG CAGTTCGGGG TCCGTCAGCT CATCCGCTGC CCAGCCGGNA 
0181 AGATCAATCC CAGGAGGAGG CGGAAGAGCA ACAGGTCTCC TCCGCATCCA GTGGCAGTGC 
0241 CGATCCGATT AGTGGCCGTC TTGTCTCTGC CGGAATTCCC GTCTCTGTGC CTCTTCCTCT 
0301 GATCCTGGCC GCTCGTAATG GACTACGAAC CGTATTGACC ATCCAGGAGC CAGCCGTGGC 
0361 CAAGGTGGGC GAGGTGGTGC AGCACGTGCC CACTGCCGTT TCGCATCAGA CCCAGACGGT 
0421 GGTGCACGAT CACCGCCGAT TGGTCACACC CATTGTGGCA CCCGCTGTTC GCACCACCCA 
0481 GGTGATTCGC CAGCAGCCAC CACT

in silico PCR Fragment

0001 GCCA--TTGT GCTGGTCTCC ATCGGAGCTT CGCATACAGC CAGCTTGGAG TGGCCCTCGA 
0061 ATCTTGTGGC TCTCAGTTCG GTCAAGTCC- TCGCAGCTGC TTCCGATTGC CAGCGAGGAT 
0121 TCCGTTGAGT TGGCCGATGG CAGTTCGGGG TCCGTCAGCT CATCCGCTGC CCAGCCGG-A 
0181 AGATCAATCC CAGGAGGAGG CGGAAGAGCA ACAGGTCTCC TCCGCATCCA GTGGCAGTGC 
0241 CGATCCGATT AGTGGCCGTC TTGTCTCTGC CGGAATTCCC GTCTCTGTGC CTCTTCCTCT 
0301 GATCCTGGCC GCTCGTAATG GACTACGAAC CGTATTGACC ATCCAGGAGC CAGCCGTGGC 
0361 CAAGGTGGGC GAGGTGGTGC AGCACGTGCC CACTGCCGTT TCGCATCAGA CCCAGACGGT 
0421 GGTGCACGAT CACCGCCGAT TGGTCACACC CATTGTGGCA CCCGCTGTTC GCACCACCCA 
0481 GGTGATTCGC CAGCAGCCAC CACT

Assemble Data

<PRE>

13057R-4.IR_full       1   GCCA<span class="snp"><tt>N</tt></span><span class="snp"><tt>T</tt></span>TTGTGCTGGTCTCCATCGGAGCTTCGCATACAGCCAGCTTGGAGTGGCCCTCGA 60
                           ||||<span class="snp"><tt>&nbsp;</tt></span><span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||
13057R-4.in silico     1   GCCA<span class="snp"><tt>-</tt></span><span class="snp"><tt>-</tt></span>TTGTGCTGGTCTCCATCGGAGCTTCGCATACAGCCAGCTTGGAGTGGCCCTCGA 60


13057R-4.IR_full       61  ATCTTGTGGCTCTCAGTTCGGTCAAGTCC<span class="snp"><tt>N</tt></span>TCGCAGCTGCTTCCGATTGCCAGCGAGGAT 120
                           |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
13057R-4.in silico     61  ATCTTGTGGCTCTCAGTTCGGTCAAGTCC<span class="snp"><tt>-</tt></span>TCGCAGCTGCTTCCGATTGCCAGCGAGGAT 120


13057R-4.IR_full       121 TCCGTTGAGTTGGCCGATGGCAGTTCGGGGTCCGTCAGCTCATCCGCTGCCCAGCCGG<span class="snp"><tt>N</tt></span>A 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|
13057R-4.in silico     121 TCCGTTGAGTTGGCCGATGGCAGTTCGGGGTCCGTCAGCTCATCCGCTGCCCAGCCGG<span class="snp"><tt>-</tt></span>A 180


13057R-4.IR_full       181 AGATCAATCCCAGGAGGAGGCGGAAGAGCAACAGGTCTCCTCCGCATCCAGTGGCAGTGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13057R-4.in silico     181 AGATCAATCCCAGGAGGAGGCGGAAGAGCAACAGGTCTCCTCCGCATCCAGTGGCAGTGC 240


13057R-4.IR_full       241 CGATCCGATTAGTGGCCGTCTTGTCTCTGCCGGAATTCCCGTCTCTGTGCCTCTTCCTCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13057R-4.in silico     241 CGATCCGATTAGTGGCCGTCTTGTCTCTGCCGGAATTCCCGTCTCTGTGCCTCTTCCTCT 300


13057R-4.IR_full       301 GATCCTGGCCGCTCGTAATGGACTACGAACCGTATTGACCATCCAGGAGCCAGCCGTGGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13057R-4.in silico     301 GATCCTGGCCGCTCGTAATGGACTACGAACCGTATTGACCATCCAGGAGCCAGCCGTGGC 360


13057R-4.IR_full       361 CAAGGTGGGCGAGGTGGTGCAGCACGTGCCCACTGCCGTTTCGCATCAGACCCAGACGGT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13057R-4.in silico     361 CAAGGTGGGCGAGGTGGTGCAGCACGTGCCCACTGCCGTTTCGCATCAGACCCAGACGGT 420


13057R-4.IR_full       421 GGTGCACGATCACCGCCGATTGGTCACACCCATTGTGGCACCCGCTGTTCGCACCACCCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13057R-4.in silico     421 GGTGCACGATCACCGCCGATTGGTCACACCCATTGTGGCACCCGCTGTTCGCACCACCCA 480


13057R-4.IR_full       481 GGTGATTCGCCAGCAGCCACCACT 504
                           ||||||||||||||||||||||||
13057R-4.in silico     481 GGTGATTCGCCAGCAGCCACCACT 504

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_080256.2	CG13057-RA (retinin), mRNA
0.62	3	2	4	2	NM_170063.2	CG10868-RD, transcript variant D (orb), mRNA
0.62	3	2	4	2	NM_170062.1	CG10868-RB, transcript variant B (orb), mRNA
0.62	3	2	4	2	NM_079736.2	CG10868-RA, transcript variant A (orb), mRNA
0.2	1	4	7	3	NM_164996.1	CG31760-RA (CG31760), mRNA
0	0	10	2	2	NM_132780.1	CG15029-RA (CG15029), mRNA
0	0	3	3	4	NM_165034.1	CG6043-RA, transcript variant A (CG6043), mRNA
0	0	3	3	4	NM_165037.1	CG6043-RC, transcript variant C (CG6043), mRNA
0	0	3	3	4	NM_165035.1	CG6043-RB, transcript variant B (CG6043), mRNA
0	0	3	3	4	NM_135785.1	CG6043-RD, transcript variant D (CG6043), mRNA
0	0	3	3	3	NM_136125.1	CG10034-RA (tj), mRNA
0	0	2	5	4	NM_001038871.1	CG34020-RA (CG34020), mRNA
0	0	2	3	4	NM_206465.1	CG11992-RD, transcript variant D (Rel), mRNA
0	0	2	3	4	NM_206466.1	CG11992-RC, transcript variant C (Rel), mRNA
0	0	2	3	4	NM_206467.1	CG11992-RB, transcript variant B (Rel), mRNA
0	0	2	3	4	NM_057746.3	CG11992-RA, transcript variant A (Rel), mRNA
0	0	2	3	2	NM_078765.2	CG9115-RA (mtm), mRNA
0	0	2	3	2	NM_079821.2	CG1954-RA (Pkc98E), mRNA
0	0	2	2	2	NM_078956.2	CG12210-RA, transcript variant A (Syb), mRNA
0	0	2	2	2	NM_165756.1	CG12210-RB, transcript variant B (Syb), mRNA
0	0	1	5	17	NM_140866.1	CG9299-RA (CG9299), mRNA
0	0	1	5	2	NM_078779.2	CG5125-RB, transcript variant B (ninaC), mRNA
0	0	1	5	2	NM_164747.1	CG5125-RA, transcript variant A (ninaC), mRNA
0	0	1	4	2	NM_139423.1	CG5690-RA (CG5690), mRNA
0	0	1	4	2	NM_135173.1	CG9501-RA (ppk14), mRNA
0	0	1	3	11	NM_167340.1	CG32648-RA (Pde9), mRNA
0	0	1	3	5	NM_140888.2	CG8743-RA (CG8743), mRNA
0	0	1	3	5	NM_136602.3	CG8083-RA, transcript variant A (CG8083), mRNA
0	0	1	3	5	NM_206060.1	CG8083-RB, transcript variant B (CG8083), mRNA
0	0	1	3	5	NM_206059.1	CG8083-RC, transcript variant C (CG8083), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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