NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

13039R-3

Symbol

CG13039

Full Name

CG13039

CG No

CG13039

Old CG No

CG13039

Synonyms

BcDNA:AT27312, CG13039

Accession No (Link to NCBI)

NM_140619.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGTCGAATACCAGTCGTCGC

Primer Seq. 3'

aatctagaggtaccGGTGCTGGATCAGGTTCTCGT

Predicted Fragment Size

IR fragment full Seq

0001 ATGTCGAATA CCAGTCGTCG CGATATTTGG GTGCCCAGTC CCGCCATTAT ACTGCATCAG 
0061 ATGCAATCCA ACGAGGATGT GTCCGATGAA TCAACTCTGG AATACCATGA GGAATCCAAC 
0121 CAGGCGTGGC GCGAACAGTA TATGGCCAAA AACGTGGCCA GNAATGGGAG TCGGTTTCGA 
0181 GTTCGTGAAC TCAACCTCTC GCTACATGAG CTCCTTGAGC AGCACCCAGG ATGACGATAC 
0241 AGAGAACCAC GTGATTGGTA ATTCATCTTC CAGCACCACC TATCGAGTGG GGCCCACCAT 
0301 CCGAATCCCC CGTCTCGAAA TTCAGTCTTA CAGTTCCGGG TCGGATGACG AGAACCTGAT 
0361 CCAGCACC

in silico PCR Fragment

0001 ATGTCGAATA CCAGTCGTCG CGATATTTGG GTGCCCAGTC CCGCCATTAT ACTGCATCAG 
0061 ATGCAATCCA ACGAGGATGT GTCCGATGAA TCAACTCTGG AATACCATGA GGAATCCAAC 
0121 CAGGCGTGGC GCGAACAGTA TATGGCCAAA AACGTGGCCA G-AATGGGAG TCGGTTTCGA 
0181 GTTCGTGAAC TCAACCTCTC GCTACATGAG CTCCTTGAGC AGCACCCAGG ATGACGATAC 
0241 AGAGAACCAC GTGATTGGTA ATTCATCTTC CAGCACCACC TATCGAGTGG GGCCCACCAT 
0301 CCGAATCCCC CGTCTCGAAA TTCAGTCTTA CAGTTCCGGG TCGGATGACG AGAACCTGAT 
0361 CCAGCACC

Assemble Data

<PRE>

13039R-3.IR_full       1   ATGTCGAATACCAGTCGTCGCGATATTTGGGTGCCCAGTCCCGCCATTATACTGCATCAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13039R-3.in silico     1   ATGTCGAATACCAGTCGTCGCGATATTTGGGTGCCCAGTCCCGCCATTATACTGCATCAG 60


13039R-3.IR_full       61  ATGCAATCCAACGAGGATGTGTCCGATGAATCAACTCTGGAATACCATGAGGAATCCAAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13039R-3.in silico     61  ATGCAATCCAACGAGGATGTGTCCGATGAATCAACTCTGGAATACCATGAGGAATCCAAC 120


13039R-3.IR_full       121 CAGGCGTGGCGCGAACAGTATATGGCCAAAAACGTGGCCAG<span class="snp"><tt>N</tt></span>AATGGGAGTCGGTTTCGA 180
                           |||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||
13039R-3.in silico     121 CAGGCGTGGCGCGAACAGTATATGGCCAAAAACGTGGCCAG<span class="snp"><tt>-</tt></span>AATGGGAGTCGGTTTCGA 180


13039R-3.IR_full       181 GTTCGTGAACTCAACCTCTCGCTACATGAGCTCCTTGAGCAGCACCCAGGATGACGATAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13039R-3.in silico     181 GTTCGTGAACTCAACCTCTCGCTACATGAGCTCCTTGAGCAGCACCCAGGATGACGATAC 240


13039R-3.IR_full       241 AGAGAACCACGTGATTGGTAATTCATCTTCCAGCACCACCTATCGAGTGGGGCCCACCAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13039R-3.in silico     241 AGAGAACCACGTGATTGGTAATTCATCTTCCAGCACCACCTATCGAGTGGGGCCCACCAT 300


13039R-3.IR_full       301 CCGAATCCCCCGTCTCGAAATTCAGTCTTACAGTTCCGGGTCGGATGACGAGAACCTGAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13039R-3.in silico     301 CCGAATCCCCCGTCTCGAAATTCAGTCTTACAGTTCCGGGTCGGATGACGAGAACCTGAT 360


13039R-3.IR_full       361 CCAGCACC 368
                           ||||||||
13039R-3.in silico     361 CCAGCACC 368

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	349	0	0	0	NM_140619.2	CG13039-RA (CG13039), mRNA
0	0	1	3	2	NM_136135.2	CG10132-RA (CG10132), mRNA
0	0	1	3	2	NM_165604.1	CG30366-RA (CG30366), mRNA
0	0	1	3	2	NM_166924.1	CG4199-RF, transcript variant F (CG4199), mRNA
0	0	1	3	2	NM_166921.1	CG4199-RE, transcript variant E (CG4199), mRNA
0	0	1	3	2	NM_166923.1	CG4199-RC, transcript variant C (CG4199), mRNA
0	0	1	3	2	NM_130620.2	CG4199-RD, transcript variant D (CG4199), mRNA
0	0	1	3	2	NM_166922.1	CG4199-RB, transcript variant B (CG4199), mRNA
0	0	1	3	2	NM_166925.1	CG4199-RA, transcript variant A (CG4199), mRNA
0	0	1	2	4	NM_170145.1	CG31128-RA (CG31128), mRNA
0	0	0	12	20	NM_135596.2	CG7300-RB, transcript variant B (CG7300), mRNA
0	0	0	7	21	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	0	7	4	NM_080535.2	CG9774-RA (rok), mRNA
0	0	0	5	3	NM_135514.2	CG4908-RA, transcript variant A (CG4908), mRNA
0	0	0	5	3	NM_164892.1	CG4908-RB, transcript variant B (CG4908), mRNA
0	0	0	5	2	NM_079395.2	CG9695-RA (Dab), mRNA
0	0	0	4	6	NM_137876.1	CG30264-RA (CG30264), mRNA
0	0	0	4	3	NM_169752.1	CG31262-RA (CG31262), mRNA
0	0	0	3	5	NM_132794.2	CG18210-RA (CG18210), mRNA
0	0	0	3	4	NM_132831.1	CG8184-RB (CG8184), mRNA
0	0	0	3	4	NM_141951.1	CG5608-RA (CG5608), mRNA
0	0	0	3	4	NM_131995.1	CG15784-RA (CG15784), mRNA
0	0	0	3	3	NM_001014545.1	CG33519-RB (Unc-89), mRNA
0	0	0	3	3	NM_057941.3	CG5227-RA, transcript variant A (sdk), mRNA
0	0	0	3	3	NM_137847.2	CG4207-RA (bonsai), mRNA
0	0	0	3	3	NM_137977.2	CG11299-RA (CG11299), mRNA
0	0	0	3	2	NM_142098.1	CG8538-RA (CG8538), mRNA
0	0	0	3	2	NM_137502.3	CG5323-RA (CG5323), mRNA
0	0	0	3	2	NM_144103.1	CG13946-RA (CG13946), mRNA
0	0	0	3	2	NM_134697.1	CG12506-RA (CG12506), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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