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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

13037R-2

Symbol

CG33257

Full Name

CG33257

CG No

CG33257

Old CG No

CG13037

Synonyms

CG13037, CG33257

Accession No (Link to NCBI)

NM_079380.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCACCACAGACTTTCGCGGCAA

Primer Seq. 3'

aatctagaggtaccGTTTCGGCTGGCCCACACCAA

Predicted Fragment Size

IR fragment full Seq

0001 CACCACAGAC TTTCGCGGCA ACACGCTGTG GGAGCTGGTC TCAAACCTGC CCAACTGGGG 
0061 AGTGGGCCGC CTGCTAATAC GTAACATGTT CCAGCGATAT NCCGGAGCCC TGCTACATGC 
0121 GGATTCTGAA GGTGCAGTCC GTGGACGAGC AGCCCGGCGA GATCCGCAAG GTGCGGGTCA 
0181 CTGTGGAGAA AACGTGGCGC GGCGTAACGC AGCCAAAGCC TGTGGAAATC TACAGCACCA 
0241 GCTATAAGGC GGACTACGAA CTGGTGCCGC AGGATCAGGA AGCAAAGTAT CTGAACAACA 
0301 AGAAGAAGGT TGAACCAGTG ATACTGCCCA CCAAGATTGA ACTTNCCGCC TCTGCTCCGC 
0361 GAACTGGTAT CGGAGGAAAC GGGCAACCCC AATCCCCAGA TGAAGGTCCA CTACAAACTG 
0421 ACCGACAACA AAATGGCCAG ATTAGCCAAG GATGGAGAAA AGCCAACTGT TAACTTTGCC 
0481 CTTGGTGTGG GCCAGcCGAA AC

in silico PCR Fragment

0001 CACCACAGAC TTTCGCGGCA ACACGCTGTG GGAGCTGGTC TCAAACCTGC CCAACTGGGG 
0061 TGTGGGCCGT CTGCTAATAC GTAACATGTT CCAGCGATAT -CCGGAGCCC TGCTACATGC 
0121 GGATTCTGAA GGTGCAGTCC GTGGACGAGC AGCCCGGCGA GATCCGCAAG GTGCGGGTCA 
0181 CTGTGGAGAA AACGTGGCGC GGCGTAACGC AGCCAAAGCC TGTGGAAATC TACAGCACCA 
0241 GCTATAAGGC GGACTACGAA CTGGTGCCGC AGGATCAGGA AGCAAAGTAT CTGAACAACA 
0301 AGAAGAAGGT TGAACCAGTG ATACTGCCCA CCAAGATTGA ACTT-CCGCC TCTGCTCCGC 
0361 GAACTGGTAT CGGAGGAAAC GGGCAACCCC AATCCCCAGA TGAAGGTCCA CTACAAACTG 
0421 ACCGACAACA AAATGGCCAG ATTAGCCAAG GATGGAGAAA AGCCAACTGT TAACTTTGCC 
0481 CTTGGTGTGG GCCAGCCGAA AC

Assemble Data

<PRE>

13037R-2.IR_full       1   CACCACAGACTTTCGCGGCAACACGCTGTGGGAGCTGGTCTCAAACCTGCCCAACTGGGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13037R-2.in silico     1   CACCACAGACTTTCGCGGCAACACGCTGTGGGAGCTGGTCTCAAACCTGCCCAACTGGGG 60


13037R-2.IR_full       61  <span class="snp"><tt>A</tt></span>GTGGGCCG<span class="snp"><tt>C</tt></span>CTGCTAATACGTAACATGTTCCAGCGATAT<span class="snp"><tt>N</tt></span>CCGGAGCCCTGCTACATGC 120
                           <span class="snp"><tt>&nbsp;</tt></span>||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||
13037R-2.in silico     61  <span class="snp"><tt>T</tt></span>GTGGGCCG<span class="snp"><tt>T</tt></span>CTGCTAATACGTAACATGTTCCAGCGATAT<span class="snp"><tt>-</tt></span>CCGGAGCCCTGCTACATGC 120


13037R-2.IR_full       121 GGATTCTGAAGGTGCAGTCCGTGGACGAGCAGCCCGGCGAGATCCGCAAGGTGCGGGTCA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13037R-2.in silico     121 GGATTCTGAAGGTGCAGTCCGTGGACGAGCAGCCCGGCGAGATCCGCAAGGTGCGGGTCA 180


13037R-2.IR_full       181 CTGTGGAGAAAACGTGGCGCGGCGTAACGCAGCCAAAGCCTGTGGAAATCTACAGCACCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13037R-2.in silico     181 CTGTGGAGAAAACGTGGCGCGGCGTAACGCAGCCAAAGCCTGTGGAAATCTACAGCACCA 240


13037R-2.IR_full       241 GCTATAAGGCGGACTACGAACTGGTGCCGCAGGATCAGGAAGCAAAGTATCTGAACAACA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13037R-2.in silico     241 GCTATAAGGCGGACTACGAACTGGTGCCGCAGGATCAGGAAGCAAAGTATCTGAACAACA 300


13037R-2.IR_full       301 AGAAGAAGGTTGAACCAGTGATACTGCCCACCAAGATTGAACTT<span class="snp"><tt>N</tt></span>CCGCCTCTGCTCCGC 360
                           ||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||
13037R-2.in silico     301 AGAAGAAGGTTGAACCAGTGATACTGCCCACCAAGATTGAACTT<span class="snp"><tt>-</tt></span>CCGCCTCTGCTCCGC 360


13037R-2.IR_full       361 GAACTGGTATCGGAGGAAACGGGCAACCCCAATCCCCAGATGAAGGTCCACTACAAACTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13037R-2.in silico     361 GAACTGGTATCGGAGGAAACGGGCAACCCCAATCCCCAGATGAAGGTCCACTACAAACTG 420


13037R-2.IR_full       421 ACCGACAACAAAATGGCCAGATTAGCCAAGGATGGAGAAAAGCCAACTGTTAACTTTGCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
13037R-2.in silico     421 ACCGACAACAAAATGGCCAGATTAGCCAAGGATGGAGAAAAGCCAACTGTTAACTTTGCC 480


13037R-2.IR_full       481 CTTGGTGTGGGCCAGCCGAAAC 502
                           ||||||||||||||||||||||
13037R-2.in silico     481 CTTGGTGTGGGCCAGCCGAAAC 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_079380.2	CG13037-RA (mRpS34), mRNA
0.41	2	2	2	3	NM_131988.1	CG3309-RA (CG3309), mRNA
0	0	3	4	2	NM_168820.1	CG32221-RA (CG32221), mRNA
0	0	2	4	4	NM_139485.1	CG12187-RA (CG12187), mRNA
0	0	2	4	2	NM_143927.2	CG9603-RA (CG9603), mRNA
0	0	1	6	3	NM_141769.2	CG4511-RA (CG4511), mRNA
0	0	1	5	2	NM_138098.2	CG3565-RA (CG3565), mRNA
0	0	1	3	6	NM_141962.1	CG6753-RA (CG6753), mRNA
0	0	1	3	4	NM_136623.1	CG8014-RA (Rme-8), mRNA
0	0	1	3	3	NM_079070.2	CG9291-RB, transcript variant B (Elongin-C), mRNA
0	0	1	3	3	NM_166354.1	CG9291-RA, transcript variant A (Elongin-C), mRNA
0	0	1	3	2	NM_138115.2	CG30421-RA (CG30421), mRNA
0	0	1	2	5	NM_140801.2	CG4076-RA (Nufip), mRNA
0	0	1	2	3	NM_140925.2	CG7323-RA (CG7323), mRNA
0	0	1	2	3	NM_139711.2	CG10626-RA (Lkr), mRNA
0	0	1	2	3	NM_169390.2	CG31374-RB, transcript variant B (CG31374), mRNA
0	0	1	2	3	NM_176460.1	CG31374-RC, transcript variant C (CG31374), mRNA
0	0	1	2	3	NM_080245.3	CG17437-RA (wds), mRNA
0	0	1	2	2	NM_136682.1	CG1671-RA (CG1671), mRNA
0	0	1	2	2	NM_140732.1	CG7571-RA (Oatp74D), mRNA
0	0	1	2	2	NM_137618.1	CG9864-RA (CG9864), mRNA
0	0	0	7	6	NM_079692.2	CG4204-RA (Elongin-B), mRNA
0	0	0	7	4	NM_137099.2	CG8494-RA (CG8494), mRNA
0	0	0	7	2	NM_131961.1	CG2861-RA, transcript variant A (CG2861), mRNA
0	0	0	5	16	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	0	4	7	NM_139491.1	CG1241-RA (Atg2), mRNA
0	0	0	4	6	NM_169883.1	CG4836-RA, transcript variant A (CG4836), mRNA
0	0	0	4	5	NM_169884.1	CG31210-RA (CG31210), mRNA
0	0	0	4	5	NM_142599.1	CG4836-RC, transcript variant C (CG4836), mRNA
0	0	0	4	5	NM_169882.1	CG4836-RB, transcript variant B (CG4836), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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