NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

12822R-1

Symbol

CG12822

Full Name

CG12822

CG No

CG12822

Old CG No

CG12822

Synonyms

CG12822

Accession No (Link to NCBI)

NM_136483.2

Inserted Chr.

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCTCAGGCAGCAAGTGCGCAA

Primer Seq. 3'

aatctagaggtaccGTGTGGAAAACACACCCACCC

Predicted Fragment Size

IR fragment full Seq

0001 TCTCAGGCAG CAAGTGCGCA ACCTGCAGCA TGTGCAGCGC AAGGACATTG AGACGATAAC 
0061 CCGCCTGCTG CAGGACTTCC GCTGCGAGGG GTGTGCGAAC AACAACAAAA GCGCCAAGGA 
0121 CAAGGTCGCC GGCGAGAAGC AGGAGCACCA GCACCAACAG CAGACGCAGG ACCAGCAGAC 
0181 GGGCCAAGGT CAGAGCAATT ATACCAATGG TGGGCACTGC TTGGGAGAGN ACTACGCACA 
0241 CTTTCGGCCC ATAGGAGTGA TCCGGACAGC ATTCCCAGAG AAGCGGGCCG TgCCGAGGCA 
0301 GTCAATTGTG GGCAGTCGcC TGCGGGGTAT CATCCAACTG AACGACGGTG TGTTCACCAA 
0361 TCCGGAGCAC TCCTTGGAGG GACTAGAGGA CTTTTCCCAT CTGTGGCTCA TCTATCATTT 
0421 TCACCGCAAC AATTCCCACC CCAAGGCCAA GGTGGCGCCT CCCCGTCTTG GTGGGGAGCG 
0481 GGTGGGTGTG TTTTCCACAC

in silico PCR Fragment

0001 TCTCAGGCAG CAAGTGCGCA ACCTGCAGCA TGTGCAGCGC AAGGACATTG AGACGATAAC 
0061 CCGCCTGCTG CAGGACTTCC GCTGCGAGGG GTGTGCGAAC AACAACAAAA GCGCCAAGGA 
0121 CAAGGTCGCC GGCGAGAAGC AGGAGCACCA GCACCAACAG CAGACGCAGG ACCAGCAGAC 
0181 GGGCCAAGGT CAGAGCAATT ATACCAATGG TGGGCACTGC TTGGGAGAGG ACTACGCACA 
0241 CTTTCGGCCC ATAGGAGTGA TCCGGACAGC ATTCCCAGAG AAGCGGGCCG TGCCGAGGCA 
0301 GTCAATTGTG GGCAGTCGCC TGCGGGGTAT CATCCAACTG AACGACGGTG TGTTCACCAA 
0361 TCCGGAGCAC TCCTTGGAGG GACTAGAGGA CTTTTCCCAT CTGTGGCTCA TCTATCATTT 
0421 TCACCGCAAC AATTCCCACC CCAAGGCCAA GGTGGCGCCT CCCCGTCTTG GTGGGGAGCG 
0481 GGTGGGTGTG TTTTCCACAC

Assemble Data

<PRE>

12822R-1.IR_full       1   TCTCAGGCAGCAAGTGCGCAACCTGCAGCATGTGCAGCGCAAGGACATTGAGACGATAAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12822R-1.in silico     1   TCTCAGGCAGCAAGTGCGCAACCTGCAGCATGTGCAGCGCAAGGACATTGAGACGATAAC 60


12822R-1.IR_full       61  CCGCCTGCTGCAGGACTTCCGCTGCGAGGGGTGTGCGAACAACAACAAAAGCGCCAAGGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12822R-1.in silico     61  CCGCCTGCTGCAGGACTTCCGCTGCGAGGGGTGTGCGAACAACAACAAAAGCGCCAAGGA 120


12822R-1.IR_full       121 CAAGGTCGCCGGCGAGAAGCAGGAGCACCAGCACCAACAGCAGACGCAGGACCAGCAGAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12822R-1.in silico     121 CAAGGTCGCCGGCGAGAAGCAGGAGCACCAGCACCAACAGCAGACGCAGGACCAGCAGAC 180


12822R-1.IR_full       181 GGGCCAAGGTCAGAGCAATTATACCAATGGTGGGCACTGCTTGGGAGAG<span class="snp"><tt>N</tt></span>ACTACGCACA 240
                           |||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||
12822R-1.in silico     181 GGGCCAAGGTCAGAGCAATTATACCAATGGTGGGCACTGCTTGGGAGAG<span class="snp"><tt>G</tt></span>ACTACGCACA 240


12822R-1.IR_full       241 CTTTCGGCCCATAGGAGTGATCCGGACAGCATTCCCAGAGAAGCGGGCCGTGCCGAGGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12822R-1.in silico     241 CTTTCGGCCCATAGGAGTGATCCGGACAGCATTCCCAGAGAAGCGGGCCGTGCCGAGGCA 300


12822R-1.IR_full       301 GTCAATTGTGGGCAGTCGCCTGCGGGGTATCATCCAACTGAACGACGGTGTGTTCACCAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12822R-1.in silico     301 GTCAATTGTGGGCAGTCGCCTGCGGGGTATCATCCAACTGAACGACGGTGTGTTCACCAA 360


12822R-1.IR_full       361 TCCGGAGCACTCCTTGGAGGGACTAGAGGACTTTTCCCATCTGTGGCTCATCTATCATTT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12822R-1.in silico     361 TCCGGAGCACTCCTTGGAGGGACTAGAGGACTTTTCCCATCTGTGGCTCATCTATCATTT 420


12822R-1.IR_full       421 TCACCGCAACAATTCCCACCCCAAGGCCAAGGTGGCGCCTCCCCGTCTTGGTGGGGAGCG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12822R-1.in silico     421 TCACCGCAACAATTCCCACCCCAAGGCCAAGGTGGCGCCTCCCCGTCTTGGTGGGGAGCG 480


12822R-1.IR_full       481 GGTGGGTGTGTTTTCCACAC 500
                           ||||||||||||||||||||
12822R-1.in silico     481 GGTGGGTGTGTTTTCCACAC 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_136483.2	CG12822-RA, transcript variant A (CG12822), mRNA
100	482	0	0	0	NM_206052.1	CG12822-RB, transcript variant B (CG12822), mRNA
90.04	434	3	1	1	NM_165566.1	CG18853-RA (CG18853), mRNA
0.2	1	2	4	4	NM_140697.2	CG7853-RA (CG7853), mRNA
0	0	5	8	10	NM_143223.1	CG14238-RA (CG14238), mRNA
0	0	4	2	45	NM_001014459.1	CG33516-RA (dpr3), mRNA
0	0	4	2	3	NM_168811.1	CG32209-RB (CG32209), mRNA
0	0	3	12	9	NM_140203.1	CG6163-RA (CG6163), mRNA
0	0	3	10	17	NM_140096.3	CG18177-RB, transcript variant B (CG18177), mRNA
0	0	3	10	17	NM_206310.1	CG18177-RA, transcript variant A (CG18177), mRNA
0	0	3	8	63	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
0	0	3	8	63	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
0	0	3	8	37	NM_167597.1	CG32494-RA (CG32494), mRNA
0	0	3	4	29	NM_167834.1	CG17090-RB, transcript variant B (CG17090), mRNA
0	0	3	4	29	NM_138194.2	CG17090-RA, transcript variant A (CG17090), mRNA
0	0	3	3	3	NM_137944.2	CG9891-RA (yellow-d2), mRNA
0	0	3	2	4	NM_132198.2	CG1530-RA (CG1530), mRNA
0	0	2	44	436	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	2	44	436	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	2	44	436	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	2	20	173	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
0	0	2	20	173	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
0	0	2	15	108	NM_078797.2	CG13109-RA (tai), mRNA
0	0	2	13	82	NM_139799.3	CG10107-RA, transcript variant A (CG10107), mRNA
0	0	2	13	82	NM_206286.1	CG10107-RC, transcript variant C (CG10107), mRNA
0	0	2	13	39	NM_079504.3	CG1133-RA (opa), mRNA
0	0	2	13	39	NM_139536.1	CG11505-RB, transcript variant B (CG11505), mRNA
0	0	2	13	39	NM_168007.1	CG11505-RA, transcript variant A (CG11505), mRNA
0	0	2	12	29	NM_080253.2	CG5067-RA (cic), mRNA
0	0	2	12	24	NM_168551.1	CG32130-RA, transcript variant A (stv), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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