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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12802R-2

Symbol CG34360 Full Name -

CG No CG34360 Old CG No CG12802

Synonyms FBgn0085389, CG12802, BcDNA:RE66512, CG33975, CG11676, CG32469, BcDNA:RH63124, CG12804, CG12805, CG34360

Accession No (Link to NCBI) NM_141703.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCACTTTTGTGGTGCCCGGCGA

Primer Seq. 3'

aatctagaggtaccAGGATGTGCCCACTCACCTCA

Predicted Fragment Size

IR fragment full Seq

0001 CACTTTTGTG GTGCCCGGCG AGGCACCGAN TTAATGCCGA TACGCGCTAC CTGGTCCGTT 
0061 TCGATTTCAA GACCGCCGTC GAGTCCCCAA CCGCTACCAC GTCGTCCGCG GCCTCGACCT 
0121 CGTCCACATC CTCCACAGAT CCGTCGGTCA TCGTGGAAAC GCGTCgCGCT GCCAACGTAC 
0181 ACATCAGTCC CGCTCAGGCA CTGCGTCGCA GCGCCATGAT CAAGGAGTTC CGCGAGTCGG 
0241 ATCTTATTGG ACCCGGATTC CGGTCCATCA TGGACACCGA ACTGCAGCCT GGCCAGCGGG 
0301 TCTACTTCAC CTACAATGGA CGCGAGCAGA GCGGCGATGT CGTCAAACAC GACGCTACCA 
0361 AGGATGAGGT GATTGTCAAG ATCACAACAG TTGGAAATGA GGTGAGTGGG CACATCCT

in silico PCR Fragment

0001 CACTTTTGTG GTGCCCGGCG AGGCACCGA- TTAATGCCGA TACGCGCTAC CTGGTCCGTT 
0061 TCGATTTCAA GACCGCCGTC GAGTCCCCAA CCGCTACCAC GTCGTCCGCG GCCTCGACCT 
0121 CGTCCACATC CTCCACAGAT CCGTCGGTCA TCGTGGAAAC GCGTCGCGCT GCCAACGTAC 
0181 ACATCAGTCC CGCTCAGGCA CTGCGTCGCA GCGCCATGAT CAAGGAGTTC CGCGAGTCGG 
0241 ATCTTATTGG ACCCGGATTC CGGTCCATCA TGGACACCGA ACTGCAGCCT GGCCAGCGGG 
0301 TCTACTTCAC CTACAATGGA CGCGAGCAGA GCGGCGATGT CGTCAAACAC GACGCTACCA 
0361 AGGATGAGGT GATTGTCAAG ATCACAACAG TTGGAAATGA GGTGAGTGGG CACATCCT

Assemble Data


12802R-2.IR_full       1   CACTTTTGTGGTGCCCGGCGAGGCACCGANTTAATGCCGATACGCGCTACCTGGTCCGTT 60
                           ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
12802R-2.in silico     1   CACTTTTGTGGTGCCCGGCGAGGCACCGA-TTAATGCCGATACGCGCTACCTGGTCCGTT 60


12802R-2.IR_full       61  TCGATTTCAAGACCGCCGTCGAGTCCCCAACCGCTACCACGTCGTCCGCGGCCTCGACCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12802R-2.in silico     61  TCGATTTCAAGACCGCCGTCGAGTCCCCAACCGCTACCACGTCGTCCGCGGCCTCGACCT 120


12802R-2.IR_full       121 CGTCCACATCCTCCACAGATCCGTCGGTCATCGTGGAAACGCGTCGCGCTGCCAACGTAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12802R-2.in silico     121 CGTCCACATCCTCCACAGATCCGTCGGTCATCGTGGAAACGCGTCGCGCTGCCAACGTAC 180


12802R-2.IR_full       181 ACATCAGTCCCGCTCAGGCACTGCGTCGCAGCGCCATGATCAAGGAGTTCCGCGAGTCGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12802R-2.in silico     181 ACATCAGTCCCGCTCAGGCACTGCGTCGCAGCGCCATGATCAAGGAGTTCCGCGAGTCGG 240


12802R-2.IR_full       241 ATCTTATTGGACCCGGATTCCGGTCCATCATGGACACCGAACTGCAGCCTGGCCAGCGGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12802R-2.in silico     241 ATCTTATTGGACCCGGATTCCGGTCCATCATGGACACCGAACTGCAGCCTGGCCAGCGGG 300


12802R-2.IR_full       301 TCTACTTCACCTACAATGGACGCGAGCAGAGCGGCGATGTCGTCAAACACGACGCTACCA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12802R-2.in silico     301 TCTACTTCACCTACAATGGACGCGAGCAGAGCGGCGATGTCGTCAAACACGACGCTACCA 360


12802R-2.IR_full       361 AGGATGAGGTGATTGTCAAGATCACAACAGTTGGAAATGAGGTGAGTGGGCACATCCT 418
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12802R-2.in silico     361 AGGATGAGGTGATTGTCAAGATCACAACAGTTGGAAATGAGGTGAGTGGGCACATCCT 418

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	399	0	0	1	NM_141703.1	CG12802-RA (CG12802), mRNA
0.5	2	4	2	2	NM_142072.2	CG9922-RA (CG9922), mRNA
0	0	4	4	3	NM_168546.1	CG10732-RA, transcript variant A (CG10732), mRNA
0	0	2	2	2	NM_138011.2	CG3803-RA (CG3803), mRNA
0	0	1	5	5	NM_079016.3	CG8523-RA (Mdr50), mRNA
0	0	1	3	4	NM_166415.1	CG3216-RB, transcript variant B (CG3216), mRNA
0	0	1	3	4	NM_137688.1	CG3216-RA, transcript variant A (CG3216), mRNA
0	0	1	3	4	NM_001038895.1	CG33966-RA (CG33966), mRNA
0	0	1	3	4	NM_080342.2	CG1787-RA (Hexo2), mRNA
0	0	1	3	3	NM_169833.1	CG7717-RB, transcript variant B (Mekk1), mRNA
0	0	1	3	3	NM_142493.2	CG7717-RA, transcript variant A (Mekk1), mRNA
0	0	1	2	5	NM_137806.2	CG5465-RA (MED16), mRNA
0	0	1	2	3	NM_080307.1	CG3206-RA (Or2a), mRNA
0	0	1	2	2	NR_001647.1	CG3206-RA (Or2a), mRNA, snRNA
0	0	0	6	23	NM_001032053.1	CG33715-RB, transcript variant B (Msp-300), mRNA
0	0	0	6	23	NM_001032051.1	CG33715-RD, transcript variant D (Msp-300), mRNA
0	0	0	6	6	NM_142463.2	CG7678-RA (CG7678), mRNA
0	0	0	6	4	NM_167654.1	CG3400-RF, transcript variant F (Pfrx), mRNA
0	0	0	6	4	NM_167651.1	CG3400-RA, transcript variant A (Pfrx), mRNA
0	0	0	6	4	NM_167653.1	CG3400-RE, transcript variant E (Pfrx), mRNA
0	0	0	6	4	NM_167656.1	CG3400-RI, transcript variant I (Pfrx), mRNA
0	0	0	6	4	NM_058104.4	CG3400-RB, transcript variant B (Pfrx), mRNA
0	0	0	6	4	NM_167655.1	CG3400-RH, transcript variant H (Pfrx), mRNA
0	0	0	6	4	NM_167652.1	CG3400-RD, transcript variant D (Pfrx), mRNA
0	0	0	6	4	NM_058103.2	CG3400-RG, transcript variant G (Pfrx), mRNA
0	0	0	5	4	NM_135111.1	CG11029-RA (CG11029), mRNA
0	0	0	5	2	NM_139361.1	CG12090-RB, transcript variant B (CG12090), mRNA
0	0	0	5	2	NM_167888.1	CG12090-RC, transcript variant C (CG12090), mRNA
0	0	0	5	2	NM_167889.1	CG12090-RA, transcript variant A (CG12090), mRNA
0	0	0	4	11	NM_144331.1	CG12508-RA (CG12508), mRNA

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