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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12753R-5

Symbol CG12753 Full Name CG12753

CG No CG12753 Old CG No CG12753

Synonyms CG12753

Accession No (Link to NCBI) NM_142285.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCACCTTAAGTACCTGTACAGC

Primer Seq. 3'

aatctagaggtaccGCAATTCTCACCATTGACTCG

Predicted Fragment Size

IR fragment full Seq

0001 CACCTTAAGT ACCTGTACAG CATTCTGGAG AAGAACACCA CCGTGTCGNA AAGTAATCGT 
0061 GGCCTGCTGG TGGAGTCGCT GCGCTGCATC GCAGAGNATC CTGATTTGGG GCGACCAGCA 
0121 TGACTCGCTG GTGTTTGACT TCTTCTTGGA GAAGAATATG CTATCGTATT TCCTGCACAT 
0181 AATGCGACAA AAGAGTGGAG GCTCGAGTTT CGTCTGCGTG CAGCTCCTTC AGACTCTCAA 
0241 CATCCTCTTT GAGAACATAC GCAACGAGAC GTCCCTGTAT TATCTGTTGA GCAACAACCA 
0301 TGTAAATTCT ATCATGGTGC ACAAGTTTGA CTTCTCCGAC GAGGATGTGA TGGGCTATTA 
0361 TATACTTTTT TTAAAGACGC TAAGTCTAAA GTTAAACACT CACACAATAC ACTTCTTTTA 
0421 CAATGAGCAC ACAAATGACT TTCCCTTGTA TACAGAGGCT ATTAAGTTCT TTAATCATCC 
0481 CGAGTCAATG GTGAGAANTG C

in silico PCR Fragment

0001 CACCTTAAGT ACCTGTACAG CATTCTGGAG AAGAACACCA CCGTGTCGGA AAGTAATCGT 
0061 GGCCTGCTGG TGGAGTCGCT GCGCTGCATC GCAGAG-ATC CTGATTTGGG GCGACCAGCA 
0121 TGACTCGCTG GTGTTTGACT TCTTCTTGGA GAAGAATATG CTATCGTATT TCCTGCACAT 
0181 AATGCGACAA AAGAGTGGAG GCTCGAGTTT CGTCTGCGTG CAGCTCCTTC AGACTCTCAA 
0241 CATCCTCTTT GAGAACATAC GCAACGAGAC GTCCCTGTAT TATCTGTTGA GCAACAACCA 
0301 TGTAAATTCT ATCATGGTGC ACAAGTTTGA CTTCTCCGAC GAGGATGTGA TGGGCTATTA 
0361 TATACTTTTT TTAAAGACGC TAAGTCTAAA GTTAAACACT CACACAATAC ACTTCTTTTA 
0421 CAATGAGCAC ACAAATGACT TTCCCTTGTA TACAGAGGCT ATTAAGTTCT TTAATCATCC 
0481 CGAGTCAATG GTGAGAATTG C

Assemble Data


12753R-5.IR_full       1   CACCTTAAGTACCTGTACAGCATTCTGGAGAAGAACACCACCGTGTCGNAAAGTAATCGT 60
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
12753R-5.in silico     1   CACCTTAAGTACCTGTACAGCATTCTGGAGAAGAACACCACCGTGTCGGAAAGTAATCGT 60


12753R-5.IR_full       61  GGCCTGCTGGTGGAGTCGCTGCGCTGCATCGCAGAGNATCCTGATTTGGGGCGACCAGCA 120
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
12753R-5.in silico     61  GGCCTGCTGGTGGAGTCGCTGCGCTGCATCGCAGAG-ATCCTGATTTGGGGCGACCAGCA 120


12753R-5.IR_full       121 TGACTCGCTGGTGTTTGACTTCTTCTTGGAGAAGAATATGCTATCGTATTTCCTGCACAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12753R-5.in silico     121 TGACTCGCTGGTGTTTGACTTCTTCTTGGAGAAGAATATGCTATCGTATTTCCTGCACAT 180


12753R-5.IR_full       181 AATGCGACAAAAGAGTGGAGGCTCGAGTTTCGTCTGCGTGCAGCTCCTTCAGACTCTCAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12753R-5.in silico     181 AATGCGACAAAAGAGTGGAGGCTCGAGTTTCGTCTGCGTGCAGCTCCTTCAGACTCTCAA 240


12753R-5.IR_full       241 CATCCTCTTTGAGAACATACGCAACGAGACGTCCCTGTATTATCTGTTGAGCAACAACCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12753R-5.in silico     241 CATCCTCTTTGAGAACATACGCAACGAGACGTCCCTGTATTATCTGTTGAGCAACAACCA 300


12753R-5.IR_full       301 TGTAAATTCTATCATGGTGCACAAGTTTGACTTCTCCGACGAGGATGTGATGGGCTATTA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12753R-5.in silico     301 TGTAAATTCTATCATGGTGCACAAGTTTGACTTCTCCGACGAGGATGTGATGGGCTATTA 360


12753R-5.IR_full       361 TATACTTTTTTTAAAGACGCTAAGTCTAAAGTTAAACACTCACACAATACACTTCTTTTA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12753R-5.in silico     361 TATACTTTTTTTAAAGACGCTAAGTCTAAAGTTAAACACTCACACAATACACTTCTTTTA 420


12753R-5.IR_full       421 CAATGAGCACACAAATGACTTTCCCTTGTATACAGAGGCTATTAAGTTCTTTAATCATCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12753R-5.in silico     421 CAATGAGCACACAAATGACTTTCCCTTGTATACAGAGGCTATTAAGTTCTTTAATCATCC 480


12753R-5.IR_full       481 CGAGTCAATGGTGAGAANTGC 501
                           ||||||||||||||||| |||
12753R-5.in silico     481 CGAGTCAATGGTGAGAATTGC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_001038969.1	CG12753-RB, transcript variant B (CG12753), mRNA
100	482	0	0	2	NM_142285.1	CG12753-RA, transcript variant A (CG12753), mRNA
0	0	3	2	2	NM_057825.3	CG2928-RA (Reg-5), mRNA
0	0	2	2	4	NM_143614.1	CG1499-RA, transcript variant A (CG1499), mRNA
0	0	2	2	4	NM_170552.1	CG1499-RB, transcript variant B (CG1499), mRNA
0	0	1	4	8	NM_135091.2	CG31989-RA (Cap-D3), mRNA
0	0	1	4	2	NM_176544.1	CG33193-RA (sav), mRNA
0	0	1	3	10	NM_057377.3	CG9949-RA, transcript variant A (sina), mRNA
0	0	1	3	8	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	1	3	8	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	1	3	8	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	1	3	5	NM_079669.2	CG7665-RA, transcript variant A (Fsh), mRNA
0	0	1	3	5	NM_169777.1	CG7665-RB, transcript variant B (Fsh), mRNA
0	0	1	3	5	NM_078831.1	CG5006-RA (Or33c), mRNA
0	0	1	3	3	NM_140498.1	CG7003-RA (CG7003), mRNA
0	0	1	2	6	NM_057353.3	CG9668-RA (Rh4), mRNA
0	0	1	2	5	NM_136603.1	CG11778-RA (CG11778), mRNA
0	0	1	2	3	NM_168474.1	CG32087-RA (CG32087), mRNA
0	0	1	2	3	NM_164555.1	CG31959-RB (CG31959), mRNA
0	0	1	2	2	NM_132584.2	CG32654-RC (CG32654), mRNA
0	0	0	6	3	NM_135313.2	CG14534-RA (CG14534), mRNA
0	0	0	5	10	NM_057531.3	CG6863-RA, transcript variant A (tok), mRNA
0	0	0	5	10	NM_170168.1	CG6863-RB, transcript variant B (tok), mRNA
0	0	0	5	6	NM_079751.3	CG31136-RA (Syx1A), mRNA
0	0	0	4	5	NM_169871.1	CG6184-RC, transcript variant C (CG6184), mRNA
0	0	0	4	5	NM_142567.1	CG6184-RA, transcript variant A (CG6184), mRNA
0	0	0	4	5	NM_169870.1	CG6184-RB, transcript variant B (CG6184), mRNA
0	0	0	4	5	NM_137721.1	CG18375-RA, transcript variant A (CG18375), mRNA
0	0	0	4	5	NM_176243.1	CG18375-RB, transcript variant B (CG18375), mRNA
0	0	0	4	4	NM_058119.3	CG18783-RA, transcript variant A (Kr-h1), mRNA

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