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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12736R-1 
 Symbol CG12736  Full Name CG12736 
 CG No CG12736  Old CG No CG12736 
 Synonyms CG12736 
 Accession No (Link to NCBI) NM_136444.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGAAGTTGTGGCGCCAATGTGA 
 Primer Seq. 3' 
aatctagaggtaccTGCCCACTTGTTTTGCCAGAA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GAAGTTGTGG CGCCAATGTG AAGCgGGGCT AGTGCGCCGT ATCAGCCAGG CACTGATTGG 
0061 ACCCGGCGCC AGTCNAGAGC CGGTGGACAG GGACCCGTCT CCTGGCCACC AGTGCGGATA 
0121 AAAATNCCGG CCCCGGGGCT GCGGGAGCTG CCCCACTGCA ATGTGGGCAC CATTGGTCAC 
0181 GTTGACCATG GTAAAACGAC CCTGACCGCC GCCATTACCA GAATCCAGTC ACAGAAGGGT 
0241 CTGGCGGAGT ACCTGTCCTA CGACCAGATT GATCGGGCGC CCGAGGAGAA NGGCGCGTGG 
0301 CNATAACTAT AAATGCTTGC CATATTGGGT ATTCCACTAC AGAACGCACC TACGCTCACA 
0361 CGGATTGCCC GGGTCATGCG GACTACATAA AGAACATGAT CTCGGGCGCC TCCCAAATGG 
0421 ATGGCGCCAT TCTAGTGGTG GCTGCCACCG ATGGTCAAAT GCCCCAGACT CGCGAGCACC 
0481 TTCTTCTGGC AAAACAANGT GGGCA 
 in silico PCR Fragment 
0001 GAAGTTGTGG CGCCAATGTG AAGCGGGGCT AGTGCGCCGT ATCAGCCAGG CACTGATTGG 
0061 ACCCGGCGCC AGTC-AGAGC CGGTGGACAG GGACCCGTCT CCTGGCCACC AGTGCGGATA 
0121 AAAAT-CCGG CCCCGGGGCT GCGGGAGCTG CCCCACTGCA ATGTGGGCAC CATTGGTCAC 
0181 GTTGACCATG GTAAAACGAC CCTGACCGCC GCCATTACCA GAATCCAGTC ACAGAAGGGT 
0241 CTGGCGGAGT ACCTGTCCTA CGACCAGATT GATCGGGCGC CCGAGGAGAA -GGCGCGTGG 
0301 C-ATAACTAT AAATGCTTGC CATATTGGGT ATTCCACTAC AGAACGCACC TACGCTCACA 
0361 CGGATTGCCC GGGTCATGCG GACTACATAA AGAACATGAT CTCGGGCGCC TCCCAAATGG 
0421 ATGGCGCCAT TCTAGTGGTG GCTGCCACCG ATGGTCAAAT GCCCCAGACT CGCGAGCACC 
0481 TTCTTCTGGC AAAACAA-GT GGGCA 
 Assemble Data 
<PRE>

12736R-1.IR_full       1   GAAGTTGTGGCGCCAATGTGAAGCGGGGCTAGTGCGCCGTATCAGCCAGGCACTGATTGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12736R-1.in silico     1   GAAGTTGTGGCGCCAATGTGAAGCGGGGCTAGTGCGCCGTATCAGCCAGGCACTGATTGG 60


12736R-1.IR_full       61  ACCCGGCGCCAGTC<span class="snp"><tt>N</tt></span>AGAGCCGGTGGACAGGGACCCGTCTCCTGGCCACCAGTGCGGATA 120
                           ||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||
12736R-1.in silico     61  ACCCGGCGCCAGTC<span class="snp"><tt>-</tt></span>AGAGCCGGTGGACAGGGACCCGTCTCCTGGCCACCAGTGCGGATA 120


12736R-1.IR_full       121 AAAAT<span class="snp"><tt>N</tt></span>CCGGCCCCGGGGCTGCGGGAGCTGCCCCACTGCAATGTGGGCACCATTGGTCAC 180
                           |||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||
12736R-1.in silico     121 AAAAT<span class="snp"><tt>-</tt></span>CCGGCCCCGGGGCTGCGGGAGCTGCCCCACTGCAATGTGGGCACCATTGGTCAC 180


12736R-1.IR_full       181 GTTGACCATGGTAAAACGACCCTGACCGCCGCCATTACCAGAATCCAGTCACAGAAGGGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12736R-1.in silico     181 GTTGACCATGGTAAAACGACCCTGACCGCCGCCATTACCAGAATCCAGTCACAGAAGGGT 240


12736R-1.IR_full       241 CTGGCGGAGTACCTGTCCTACGACCAGATTGATCGGGCGCCCGAGGAGAA<span class="snp"><tt>N</tt></span>GGCGCGTGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||
12736R-1.in silico     241 CTGGCGGAGTACCTGTCCTACGACCAGATTGATCGGGCGCCCGAGGAGAA<span class="snp"><tt>-</tt></span>GGCGCGTGG 300


12736R-1.IR_full       301 C<span class="snp"><tt>N</tt></span>ATAACTATAAATGCTTGCCATATTGGGTATTCCACTACAGAACGCACCTACGCTCACA 360
                           |<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12736R-1.in silico     301 C<span class="snp"><tt>-</tt></span>ATAACTATAAATGCTTGCCATATTGGGTATTCCACTACAGAACGCACCTACGCTCACA 360


12736R-1.IR_full       361 CGGATTGCCCGGGTCATGCGGACTACATAAAGAACATGATCTCGGGCGCCTCCCAAATGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12736R-1.in silico     361 CGGATTGCCCGGGTCATGCGGACTACATAAAGAACATGATCTCGGGCGCCTCCCAAATGG 420


12736R-1.IR_full       421 ATGGCGCCATTCTAGTGGTGGCTGCCACCGATGGTCAAATGCCCCAGACTCGCGAGCACC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12736R-1.in silico     421 ATGGCGCCATTCTAGTGGTGGCTGCCACCGATGGTCAAATGCCCCAGACTCGCGAGCACC 480


12736R-1.IR_full       481 TTCTTCTGGCAAAACAA<span class="snp"><tt>N</tt></span>GTGGGCA 505
                           |||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||
12736R-1.in silico     481 TTCTTCTGGCAAAACAA<span class="snp"><tt>-</tt></span>GTGGGCA 505

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_136444.2  CG12736-RA (CG12736), mRNA 
0.41   2  23  21  46  NM_080013.2  CG6050-RA (EfTuM), mRNA 
0   0  2  3  4  NM_136533.1  CG11641-RA (pdm3), mRNA 
0   0  2  3  4  NM_079012.2  CG8085-RB, transcript variant B (RN-tre), mRNA 
0   0  2  3  3  NM_167094.1  CG4532-RE, transcript variant E (pod1), mRNA 
0   0  2  3  3  NM_132124.4  CG4532-RD, transcript variant D (pod1), mRNA 
0   0  2  3  3  NM_167093.1  CG4532-RC, transcript variant C (pod1), mRNA 
0   0  2  3  3  NM_167092.1  CG4532-RB, transcript variant B (pod1), mRNA 
0   0  2  3  3  NM_167091.1  CG4532-RA, transcript variant A (pod1), mRNA 
0   0  2  2  2  NM_001038751.1  CG2081-RC, transcript variant C (Vago), mRNA 
0   0  2  2  2  NM_132452.2  CG2081-RB, transcript variant B (Vago), mRNA 
0   0  1  6  2  NM_139532.1  CG14967-RA (CG14967), mRNA 
0   0  1  5  5  NM_132598.2  CG3989-RA (ade5), mRNA 
0   0  1  5  3  NM_142646.1  CG5483-RA (CG5483), mRNA 
0   0  1  4  3  NM_143149.1  CG5071-RB, transcript variant B (CG5071), mRNA 
0   0  1  4  3  NM_170232.1  CG5071-RA, transcript variant A (CG5071), mRNA 
0   0  1  3  4  NM_080251.2  CG12833-RB, transcript variant B (esn), mRNA 
0   0  1  3  3  NM_165507.1  CG12833-RA, transcript variant A (esn), mRNA 
0   0  1  3  2  NM_133093.1  CG6873-RA (CG6873), mRNA 
0   0  1  2  3  NM_080341.2  CG12737-RA, transcript variant A (Crag), mRNA 
0   0  1  2  3  NM_167164.2  CG12737-RB, transcript variant B (Crag), mRNA 
0   0  1  2  3  NM_139375.1  CG12105-RA (CG12105), mRNA 
0   0  0  7  2  NM_141854.2  CG14722-RA (CG14722), mRNA 
0   0  0  5  9  NM_134770.1  CG17657-RA (CG17657), mRNA 
0   0  0  5  6  NM_165777.2  CG12891-RB, transcript variant B (CPTI), mRNA 
0   0  0  5  6  NM_078961.3  CG12891-RA, transcript variant A (CPTI), mRNA 
0   0  0  5  4  NM_136390.2  CG3420-RA (CG3420), mRNA 
0   0  0  4  5  NM_139423.1  CG5690-RA (CG5690), mRNA 
0   0  0  4  3  NM_166316.1  CG12758-RC, transcript variant C (sano), mRNA 
0   0  0  4  3  NM_079064.2  CG12758-RD, transcript variant D (sano), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

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