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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

12699R-1

Symbol

CG12699

Full Name

CG12699

CG No

CG12699

Old CG No

CG12699

Synonyms

BEST:GH14656, bs24f01.y1, NEST:bs24f01, anon-WO0140519.144, CG12699

Accession No (Link to NCBI)

NM_137358.2

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGTCAGAAGAAGAAGCCCCA

Primer Seq. 3'

aatctagaggtaccGCAGTCAGTAGAGACTTCGAT

Predicted Fragment Size

IR fragment full Seq

0001 ATGTCAGAAG AAGAAGCCCC AGGATCTCCG GGTGAGGCTG TTGAAGAAGA AGATACCACT 
0061 ACATCCGATG CTGATACAAC CACAGCTGGT GCCCGCATGA TGATGATCCA CCCATGGCTG 
0121 ACGGCCGCCG CAGTGGCCAT GTATGCCACC AAGGTCATGT GGGTCAAGTT CCGTGAGATC 
0181 GGCCTGGCTA AACAGGAAAA ACGGCTCAAA AATCAACTGG CGAAGCGGTC TAAGCCCTTG 
0241 CAGACTATTG ATGAAGATGT TTCCGAATCT GATGCAGAAT Atgatgcaga atcTGATGCA 
0301 GAATCTGATG CAGAATCAGA TGCAGAGGAC GAAGACGTCG TCGGGCCCCA AGTCGAAGAT 
0361 GCAGCAGAGG GACCGGGATT CGAGCCAAAC CGTGTGGATA TATGCCATCG CCCGATCGAA 
0421 GTCTCTACTG ACTGC

in silico PCR Fragment

0001 ATGTCAGAAG AAGAAGCCCC AGGATCTCCG GGTGAGGCTG TTGAAGAAGA AGATACCACT 
0061 ACATCCGATG CTGATACAAC CACAGCTGGT GCCCGCATGA TGATGATCCA CCCATGGCTG 
0121 ACGGCCGCCG CAGTGGCCAT GTATGCCACC AAGGTCATGT GGGTCAAGTT CCGTGAGATC 
0181 GGCCTGGCTA AACAGGAAAA ACGGCTCAAA AATCAACTGG CGAAGCAGTC TAAGCCCTTG 
0241 CAGACTATTG ATGAAGATGT TTCCGAATCT GATGCAGAAT ATGATGCAGA ATCTGATGCA 
0301 GAATCTGATG CAGAATCAGA TGCAGAGGAC GAAGACGTCG TCGGGCCCCA AGTCGAAGAT 
0361 GCAGCAGAGG GACCGGGATT CGAGCCAAAC CGTGTGGATA TATGCCATCG CCCGATCGAA 
0421 GTCTCTACTG ACTGC

Assemble Data

<PRE>

12699R-1.IR_full       1   ATGTCAGAAGAAGAAGCCCCAGGATCTCCGGGTGAGGCTGTTGAAGAAGAAGATACCACT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12699R-1.in silico     1   ATGTCAGAAGAAGAAGCCCCAGGATCTCCGGGTGAGGCTGTTGAAGAAGAAGATACCACT 60


12699R-1.IR_full       61  ACATCCGATGCTGATACAACCACAGCTGGTGCCCGCATGATGATGATCCACCCATGGCTG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12699R-1.in silico     61  ACATCCGATGCTGATACAACCACAGCTGGTGCCCGCATGATGATGATCCACCCATGGCTG 120


12699R-1.IR_full       121 ACGGCCGCCGCAGTGGCCATGTATGCCACCAAGGTCATGTGGGTCAAGTTCCGTGAGATC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12699R-1.in silico     121 ACGGCCGCCGCAGTGGCCATGTATGCCACCAAGGTCATGTGGGTCAAGTTCCGTGAGATC 180


12699R-1.IR_full       181 GGCCTGGCTAAACAGGAAAAACGGCTCAAAAATCAACTGGCGAAGC<span class="snp"><tt>G</tt></span>GTCTAAGCCCTTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||
12699R-1.in silico     181 GGCCTGGCTAAACAGGAAAAACGGCTCAAAAATCAACTGGCGAAGC<span class="snp"><tt>A</tt></span>GTCTAAGCCCTTG 240


12699R-1.IR_full       241 CAGACTATTGATGAAGATGTTTCCGAATCTGATGCAGAATATGATGCAGAATCTGATGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12699R-1.in silico     241 CAGACTATTGATGAAGATGTTTCCGAATCTGATGCAGAATATGATGCAGAATCTGATGCA 300


12699R-1.IR_full       301 GAATCTGATGCAGAATCAGATGCAGAGGACGAAGACGTCGTCGGGCCCCAAGTCGAAGAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12699R-1.in silico     301 GAATCTGATGCAGAATCAGATGCAGAGGACGAAGACGTCGTCGGGCCCCAAGTCGAAGAT 360


12699R-1.IR_full       361 GCAGCAGAGGGACCGGGATTCGAGCCAAACCGTGTGGATATATGCCATCGCCCGATCGAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12699R-1.in silico     361 GCAGCAGAGGGACCGGGATTCGAGCCAAACCGTGTGGATATATGCCATCGCCCGATCGAA 420


12699R-1.IR_full       421 GTCTCTACTGACTGC 435
                           |||||||||||||||
12699R-1.in silico     421 GTCTCTACTGACTGC 435

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
102.87	429	70	56	31	NM_137358.2	CG12699-RA (CG12699), mRNA
0.95	4	2	2	3	NM_138989.2	CG17174-RA (ACXB), mRNA
0	0	3	3	3	NM_058088.3	CG8730-RA (drosha), mRNA
0	0	2	10	34	NM_057675.3	CG8169-RA (Pms2), mRNA
0	0	2	6	9	NM_142339.1	CG5169-RA (CG5169), mRNA
0	0	2	6	7	NM_134684.2	CG4184-RA (MED15), mRNA
0	0	2	5	20	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	1	4	2	NM_140973.1	CG5955-RA (CG5955), mRNA
0	0	1	3	6	NM_176565.1	CG31092-RB, transcript variant B (LpR2), mRNA
0	0	1	3	6	NM_170239.2	CG31092-RA, transcript variant A (LpR2), mRNA
0	0	1	3	3	NM_165046.1	CG9239-RB, transcript variant B (B4), mRNA
0	0	1	3	3	NM_057977.3	CG9239-RA, transcript variant A (B4), mRNA
0	0	1	2	8	NM_169929.1	CG17838-RF, transcript variant F (CG17838), mRNA
0	0	1	2	5	NM_135082.2	CG6604-RA (H15), mRNA
0	0	1	2	4	NM_142913.1	CG10254-RA, transcript variant A (CG10254), mRNA
0	0	1	2	4	NM_170081.1	CG10254-RB, transcript variant B (CG10254), mRNA
0	0	1	2	2	NM_136069.2	CG10600-RA (CG10600), mRNA
0	0	1	2	2	NM_079942.3	CG10295-RB, transcript variant B (Pak), mRNA
0	0	1	2	2	NM_166506.1	CG13502-RB, transcript variant B (CG13502), mRNA
0	0	1	2	2	NM_137793.1	CG13502-RA, transcript variant A (CG13502), mRNA
0	0	1	2	2	NM_169137.1	CG10295-RC, transcript variant C (Pak), mRNA
0	0	1	2	2	NM_169136.1	CG10295-RA, transcript variant A (Pak), mRNA
0	0	0	9	36	NR_001929.1	CR32314, miscRNA
0	0	0	9	13	NM_135786.1	CG6108-RA (CG6108), mRNA
0	0	0	8	35	NM_137357.3	CG18469-RA (CG18469), mRNA
0	0	0	8	14	NM_135611.1	CG18561-RA (CG18561), mRNA
0	0	0	6	15	NM_079383.2	CG4531-RA (argos), mRNA
0	0	0	5	5	NM_166664.1	CG4057-RA, transcript variant A (tamo), mRNA
0	0	0	5	5	NM_138045.2	CG4057-RB, transcript variant B (tamo), mRNA
0	0	0	5	3	NM_142663.3	CG15695-RA (CG15695), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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