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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

12607R-1

Symbol

CG12607

Full Name

CG12607

CG No

CG12607

Old CG No

CG12607

Synonyms

BcDNA:RE67729, CG12607

Accession No (Link to NCBI)

NM_139646.1

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTTTGCTATTGGCTTTGGCCC

Primer Seq. 3'

aatctagaggtaccTTCGTTATAGACTGGACGGCG

Predicted Fragment Size

IR fragment full Seq

0001 TTTTGCTATT GGCTTTGGcC CTTGTGGGCA TTGCTGCTGG AGCTCAGCTT CCTGACTCCG 
0061 CCACCCAGGG ACCCAATCCT CAGGATATTG CCACCCCGGA GCCGGAGTAC ATTGATATCG 
0121 ACGAACCTGC ACCGGTGGCT GCCGCACCTG CTCCTCGTCC TGTGGCCGCT GCTCCCCGTC 
0181 CCGTCTTCGC CGCCCCTGCT CCCATCGCTC GGCCAGTTGC TCATCCAGTG GCTCGCCCCG 
0241 TGGTTGTGGC CCAATCCTTC GTCCAGCAGC CCGTCCAGCA GCAGATTGTC CAGAGGGCTC 
0301 AGTACGTGGC GCCGGTGGCT CAGCAGGTGG TGTTGCCGCA ACAGCAGCTG GTGGGACACA 
0361 CCTACAACAG CAGGGCTGGA TACCAGTACC GCCGTCCAGT CTATAACGAA

in silico PCR Fragment

0001 TTTTGCTATT GGCTTTGGCC CTTGTGGGCA TTGCTGCTGG AGCTCAGCTT CCTGACTCCG 
0061 CCACCCAGGG ACCCAATCCT CAGGATATTG CCACCCCGGA GCCGGAGTAC ATTGATATCG 
0121 ACGAACCTGC ACCGGTGGCT GCCGCACCTG CTCCTCGTCC TGTGGCCGCT GCTCCCCGTC 
0181 CCGTCTTCGC CGCCCCTGCT CCCATCGCTC GGCCAGTTGC TCATCCAGTG GCTCGCCCCG 
0241 TGGTTGTGGC CCAATCCTTC GTCCAGCAGC CCGTCCAGCA GCAGATTGTC CAGAGGGCTC 
0301 AGTACGTGGC GCCGGTGGCT CAGCAGGTGG TGTTGCCGCA ACAGCAGCTG GTGGGACACA 
0361 CCTACAACAG CAGGGCTGGA TACCAGTACC GCCGTCCAGT CTATAACGAA

Assemble Data

<PRE>

12607R-1.IR_full       1   TTTTGCTATTGGCTTTGGCCCTTGTGGGCATTGCTGCTGGAGCTCAGCTTCCTGACTCCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12607R-1.in silico     1   TTTTGCTATTGGCTTTGGCCCTTGTGGGCATTGCTGCTGGAGCTCAGCTTCCTGACTCCG 60


12607R-1.IR_full       61  CCACCCAGGGACCCAATCCTCAGGATATTGCCACCCCGGAGCCGGAGTACATTGATATCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12607R-1.in silico     61  CCACCCAGGGACCCAATCCTCAGGATATTGCCACCCCGGAGCCGGAGTACATTGATATCG 120


12607R-1.IR_full       121 ACGAACCTGCACCGGTGGCTGCCGCACCTGCTCCTCGTCCTGTGGCCGCTGCTCCCCGTC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12607R-1.in silico     121 ACGAACCTGCACCGGTGGCTGCCGCACCTGCTCCTCGTCCTGTGGCCGCTGCTCCCCGTC 180


12607R-1.IR_full       181 CCGTCTTCGCCGCCCCTGCTCCCATCGCTCGGCCAGTTGCTCATCCAGTGGCTCGCCCCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12607R-1.in silico     181 CCGTCTTCGCCGCCCCTGCTCCCATCGCTCGGCCAGTTGCTCATCCAGTGGCTCGCCCCG 240


12607R-1.IR_full       241 TGGTTGTGGCCCAATCCTTCGTCCAGCAGCCCGTCCAGCAGCAGATTGTCCAGAGGGCTC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12607R-1.in silico     241 TGGTTGTGGCCCAATCCTTCGTCCAGCAGCCCGTCCAGCAGCAGATTGTCCAGAGGGCTC 300


12607R-1.IR_full       301 AGTACGTGGCGCCGGTGGCTCAGCAGGTGGTGTTGCCGCAACAGCAGCTGGTGGGACACA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12607R-1.in silico     301 AGTACGTGGCGCCGGTGGCTCAGCAGGTGGTGTTGCCGCAACAGCAGCTGGTGGGACACA 360


12607R-1.IR_full       361 CCTACAACAGCAGGGCTGGATACCAGTACCGCCGTCCAGTCTATAACGAA 410
                           ||||||||||||||||||||||||||||||||||||||||||||||||||
12607R-1.in silico     361 CCTACAACAGCAGGGCTGGATACCAGTACCGCCGTCCAGTCTATAACGAA 410

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	392	0	0	5	NM_139646.1	CG12607-RB (CG12607), mRNA
0	0	4	4	5	NM_164497.1	CG2903-RA, transcript variant A (Hrs), mRNA
0	0	4	4	5	NM_080360.3	CG2903-RB, transcript variant B (Hrs), mRNA
0	0	4	4	5	NM_164496.1	CG2903-RC, transcript variant C (Hrs), mRNA
0	0	4	3	17	NM_167544.1	CG9623-RA, transcript variant A (if), mRNA
0	0	4	3	17	NM_078654.2	CG9623-RB, transcript variant B (if), mRNA
0	0	3	4	4	NM_141591.1	CG11966-RA (CG11966), mRNA
0	0	3	3	2	NM_166435.1	CG10069-RC, transcript variant C (CG10069), mRNA
0	0	3	3	2	NM_166434.1	CG10069-RB, transcript variant B (CG10069), mRNA
0	0	3	3	2	NM_137726.2	CG10069-RA, transcript variant A (CG10069), mRNA
0	0	2	5	12	NM_078797.2	CG13109-RA (tai), mRNA
0	0	2	5	3	NM_140557.3	CG6114-RA (CG6114), mRNA
0	0	2	4	14	NM_140605.1	CG13047-RA (CG13047), mRNA
0	0	2	4	4	NM_057638.3	CG14472-RA (poe), mRNA
0	0	2	3	13	NM_140792.1	CG6896-RA (MYPT-75D), mRNA
0	0	2	3	5	NM_142684.2	CG5745-RA (CG5745), mRNA
0	0	2	3	5	NM_137247.1	CG8434-RA (lbk), mRNA
0	0	2	3	3	NM_135121.2	CG8965-RA (CG8965), mRNA
0	0	2	3	2	NM_132817.1	CG9215-RA (CG9215), mRNA
0	0	2	2	11	NM_141993.2	CG7518-RB, transcript variant B (CG7518), mRNA
0	0	2	2	11	NM_169489.1	CG7518-RA, transcript variant A (CG7518), mRNA
0	0	2	2	6	NM_139519.2	CG14962-RA (CG14962), mRNA
0	0	2	2	6	NM_137750.1	CG10321-RA (CG10321), mRNA
0	0	2	2	5	NM_132719.2	CG32604-RA, transcript variant A (l(1)G0007), mRNA
0	0	2	2	4	NM_132393.2	CG2221-RA (l(1)G0289), mRNA
0	0	1	8	7	NM_132660.2	CG1716-RA (CG1716), mRNA
0	0	1	6	5	NM_137763.3	CG17922-RA (CG17922), mRNA
0	0	1	5	10	NM_169785.1	CG7223-RC, transcript variant C (htl), mRNA
0	0	1	5	10	NM_169784.1	CG7223-RA, transcript variant A (htl), mRNA
0	0	1	5	10	NM_079670.2	CG7223-RB, transcript variant B (htl), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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