NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

1259R-1

Symbol

Cpr64Ad

Full Name

Cuticular protein 64Ad

CG No

CG1259

Old CG No

CG1259

Synonyms

Cpr64Ad, CG1259

Accession No (Link to NCBI)

NM_139618.2

Inserted Chr.

lll

Insertional Mutation

lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

lethal

Map Viewer

Reference
[Please submit your publication]

Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.<br> An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.<br> Zoolog Sci (2016) 33(6) 583-591 [ PubMed ID = 27927092 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTCTGGGTGCTCCTCTGGCTTA

Primer Seq. 3'

aatctagaggtaccCGTTGATGGAGTCGGCGTAGT

Predicted Fragment Size

IR fragment full Seq

0001 TCTGGGTGCT CCTCTGGCTT ACTCCGCCCC TCTGGGACCT GCTCCATACT TCgNCCCGGC 
0061 TGCGTACTCC GCCCCTCTGG GTTATGCGGC TCCTTTGGGA TACAACGCCC CTCTGGTGGC 
0121 GGGACCTGCT CCCCTGGTGT CCAAGACCTA CGCTGCCGCT CCTTTCGCCG CTCCCTTCGC 
0181 TGCTCCAGTT GCCCCAGTTG CTGCTCGCCT GGCTGCCCCA GTCGCCGCAC CTCTCGCTGC 
0241 CCCAGTTGCT CCAGTTGCCG CTCCCCTGGC TGCTCCAGTT GCTGCTCCCC TGGCTGCTCC 
0301 AGTTGCTGCT CCCATCGCCA CCGAGATCGT GGATGCCCAC CCACAGTACA AGTTCGCCTA 
0361 CGATGTCCAG GATACGCTGA CCGGTGACTC CAAGACCCAG GAGGAGACTC GTGATGGTGA 
0421 TGTCGTCCGT GGATCCTACT CTCTGATCGA GCCCGATGGT TCCCGTCGTA TTGTCAGCTA 
0481 CTACGCCGAC TCCATCAACG

in silico PCR Fragment

0001 TCTGGGTGCT CCTCTGGCTT ACTCCGCCCC TCTGGGACCT GCTCCATACT TCGCCCCGGC 
0061 TGCGTACTCC GCCCCTCTGG GCTACGCGGC TCCTTTGGGA TACAACGCCC CTCTGGTGGC 
0121 GGGACCTGCT CCCCTGGTGT CCAAGACCTA CGCTGCCGCT CCTTTCGCCG CTCCCTTCGC 
0181 TGCTCCAGTT GCCCCAGTTG CTGCTCGCCT GGCTGCCCCA GTCGCCGCTC CTCTCGCTGC 
0241 CCCAGTTGCT CCAGTAGCCG CTCCCCTGGC TGCTCCAGTT GCTGCTCCCC TGGCTGCTCC 
0301 AGTTGCTGCT CCCATCGCCA CCGAGATCGT GGATGCCCAC CCACAGTACA AGTTCGCCTA 
0361 CGATGTCCAG GATACGCTAA CCGGTGACTC CAAGACCCAG GAGGAGACTC GTGATGGTGA 
0421 TGTCGTCCGT GGATCCTACT CTCTGATCGA GCCCGATGGT TCCCGTCGTA TTGTCAGCTA 
0481 CTACGCCGAC TCCATCAACG

Assemble Data

<PRE>

1259R-1.IR_full       1   TCTGGGTGCTCCTCTGGCTTACTCCGCCCCTCTGGGACCTGCTCCATACTTCG<span class="snp"><tt>N</tt></span>CCCGGC 60
                          |||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||
1259R-1.in silico     1   TCTGGGTGCTCCTCTGGCTTACTCCGCCCCTCTGGGACCTGCTCCATACTTCG<span class="snp"><tt>C</tt></span>CCCGGC 60


1259R-1.IR_full       61  TGCGTACTCCGCCCCTCTGGG<span class="snp"><tt>T</tt></span>TA<span class="snp"><tt>T</tt></span>GCGGCTCCTTTGGGATACAACGCCCCTCTGGTGGC 120
                          |||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||
1259R-1.in silico     61  TGCGTACTCCGCCCCTCTGGG<span class="snp"><tt>C</tt></span>TA<span class="snp"><tt>C</tt></span>GCGGCTCCTTTGGGATACAACGCCCCTCTGGTGGC 120


1259R-1.IR_full       121 GGGACCTGCTCCCCTGGTGTCCAAGACCTACGCTGCCGCTCCTTTCGCCGCTCCCTTCGC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1259R-1.in silico     121 GGGACCTGCTCCCCTGGTGTCCAAGACCTACGCTGCCGCTCCTTTCGCCGCTCCCTTCGC 180


1259R-1.IR_full       181 TGCTCCAGTTGCCCCAGTTGCTGCTCGCCTGGCTGCCCCAGTCGCCGC<span class="snp"><tt>A</tt></span>CCTCTCGCTGC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||
1259R-1.in silico     181 TGCTCCAGTTGCCCCAGTTGCTGCTCGCCTGGCTGCCCCAGTCGCCGC<span class="snp"><tt>T</tt></span>CCTCTCGCTGC 240


1259R-1.IR_full       241 CCCAGTTGCTCCAGT<span class="snp"><tt>T</tt></span>GCCGCTCCCCTGGCTGCTCCAGTTGCTGCTCCCCTGGCTGCTCC 300
                          |||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||||||||||||||||
1259R-1.in silico     241 CCCAGTTGCTCCAGT<span class="snp"><tt>A</tt></span>GCCGCTCCCCTGGCTGCTCCAGTTGCTGCTCCCCTGGCTGCTCC 300


1259R-1.IR_full       301 AGTTGCTGCTCCCATCGCCACCGAGATCGTGGATGCCCACCCACAGTACAAGTTCGCCTA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1259R-1.in silico     301 AGTTGCTGCTCCCATCGCCACCGAGATCGTGGATGCCCACCCACAGTACAAGTTCGCCTA 360


1259R-1.IR_full       361 CGATGTCCAGGATACGCT<span class="snp"><tt>G</tt></span>ACCGGTGACTCCAAGACCCAGGAGGAGACTCGTGATGGTGA 420
                          ||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||
1259R-1.in silico     361 CGATGTCCAGGATACGCT<span class="snp"><tt>A</tt></span>ACCGGTGACTCCAAGACCCAGGAGGAGACTCGTGATGGTGA 420


1259R-1.IR_full       421 TGTCGTCCGTGGATCCTACTCTCTGATCGAGCCCGATGGTTCCCGTCGTATTGTCAGCTA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1259R-1.in silico     421 TGTCGTCCGTGGATCCTACTCTCTGATCGAGCCCGATGGTTCCCGTCGTATTGTCAGCTA 480


1259R-1.IR_full       481 CTACGCCGACTCCATCAACG 500
                          ||||||||||||||||||||
1259R-1.in silico     481 CTACGCCGACTCCATCAACG 500

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
104.97	506	24	121	251	NM_139618.2	CG1259-RB (CG1259), mRNA
2.28	11	4	7	14	NM_141421.1	CG1331-RA (Ccp84Af), mRNA
1.45	7	15	62	111	NM_168657.1	CG13049-RA, transcript variant A (CG13049), mRNA
1.45	7	15	62	111	NM_140603.1	CG13049-RB, transcript variant B (CG13049), mRNA
0	0	12	45	93	NM_140855.1	CG14095-RA (CG14095), mRNA
0	0	10	33	63	NM_168795.1	CG32214-RA (CG32214), mRNA
0	0	9	37	70	NM_168796.1	CG32208-RA (825-Oak), mRNA
0	0	9	37	70	NM_168797.1	CG32213-RA (CG32213), mRNA
0	0	8	31	47	NM_140857.2	CG12519-RA (CG12519), mRNA
0	0	8	26	66	NM_140858.2	CG18294-RA (CG18294), mRNA
0	0	8	24	36	NM_140856.1	CG14096-RA (CG14096), mRNA
0	0	7	48	158	NM_140605.1	CG13047-RA (CG13047), mRNA
0	0	7	10	13	NM_141424.2	CG1327-RA (Ccp84Ac), mRNA
0	0	6	4	12	NM_001042891.1	CG31761-RE, transcript variant E (bru-2), mRNA
0	0	6	4	10	NM_135715.2	CG31761-RA, transcript variant A (bru-2), mRNA
0	0	6	4	10	NM_176025.1	CG31761-RD, transcript variant D (bru-2), mRNA
0	0	6	4	10	NM_165007.1	CG31761-RC, transcript variant C (bru-2), mRNA
0	0	6	2	23	NM_140610.2	CG13044-RA (CG13044), mRNA
0	0	5	16	26	NM_141423.2	CG2341-RA (Ccp84Ad), mRNA
0	0	5	14	17	NM_132038.1	CG4052-RA (CG4052), mRNA
0	0	4	32	52	NM_206398.1	CG33255-RA (CG33255), mRNA
0	0	4	31	40	NR_001975.1	CR32205, miscRNA
0	0	4	10	15	NM_078517.2	CG2175-RA, transcript variant A (dec-1), mRNA
0	0	4	10	15	NM_167133.1	CG2175-RC, transcript variant C (dec-1), mRNA
0	0	4	10	15	NM_167132.1	CG2175-RB, transcript variant B (dec-1), mRNA
0	0	4	9	23	NM_206286.1	CG10107-RC, transcript variant C (CG10107), mRNA
0	0	4	9	23	NM_139799.3	CG10107-RA, transcript variant A (CG10107), mRNA
0	0	4	9	8	NM_176292.1	CG10107-RB, transcript variant B (CG10107), mRNA
0	0	4	8	26	NM_078717.2	CG3696-RA, transcript variant A (kis), mRNA
0	0	4	6	4	NM_165248.1	CG31787-RA (CG31787), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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