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Notice:
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Suspension of shipments during Golden Week Holidays: From 29 April to 13 May, deadline 16 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1258R-1 |
| Symbol |
pav |
Full Name |
pavarotti |
| CG No |
CG1258 |
Old CG No |
CG1258 |
| Synonyms |
2A12, Pav-KLP, group D, pav-Klp, Pav-klp, PAV/KLP, PAV-KLP, Pav, KIF23, CG1258, anon2A12, DmKLP1/pav, PAV-MKLP, DmMKLP1, 2A12MEL, 0973/08, 0934/13, Mklp1, anon-EST:fe2A12, pav, Pav KLP |
| Accession No (Link to NCBI) |
NM_057677.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.<br> A Targeted <i>RNAi</i> Screen Reveals <i>Drosophila</i> Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.<br> G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Perkins AD, Lee MJ, Tanentzapf G.<br> The systematic identification of cytoskeletal genes required for Drosophila melanogaster muscle maintenance.<br> Sci Data (2014) 1 140002
[
PubMed ID = 25977760
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAACTCCGCGGGTGCATCAAAC |
| Primer Seq. 3' |
aatctagaggtaccCTTCGGACAGGATCTCGAAGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AACTCCGCGG GTGCATCAAA CGGTGGAGAA ACAGCGACGA GACACCTCCG ACAAGGCCAG
0061 GGATCCAGTG AATGTGTTCT GTCGAGTGCG GCCACTACAA TCCGACGCAG ATCTCACCTC
0121 CCTAAGGGTC AAGAACTCCA CGACGATCGC ACTGAATCCG CAGGATCAGC TGCTGCAGCA
0181 TCACAAGCCA CACAACGGCG CCCAGCGCGA GGNTTCAGTA CATATTCAAG CACGTTTTCC
0241 AGCCGGATGC CACGCAGCAG GATGTGTACG CAGCTGTGGC CCAGCCGTTG GTGGNAGAAC
0301 CTACTGAAAG GCCGAAACAG TCTGCTTTTC AcCTACGGCG TGACTGGAAG TGGAAAAACG
0361 TACACCATGA CCGGCAACCT GCGAcacNGN GGCATCATGC CgNGCTGCCT GGACGTGCTC
0421 TTTCGCACCA TTTCAGATTA CCAGGCCAAG AAGTTCGTCT TCAAGCCGGA CAAGCTTAAT
0481 GGCTTCGAGA TCCTGTCCGA AG |
| in silico PCR Fragment |
0001 AACTCCGCGG GTGCATCAAA CGGTGGAGAA ACAGCGACGA GACACCTCCG ACAAGGCCAG
0061 GGATCCAGTG AATGTGTTCT GTCGAGTGCG GCCACTACAA TCCGACGCAG ATCTCACCTC
0121 CCTAAGGGTC AAGAACTCCA CGACGATCGC ACTGAATCCG CAGGATCAGC TGCTGCAGCA
0181 TCACAAGCCA CACAACGGCG CCCAGCGCGA GG-TTCAGTA CATATTCAAG CACGTTTTCC
0241 AGCCGGATGC CACGCAGCAG GATGTGTACG CAGCTGTGGC CCAGCCGTTG GTGG-AGAAC
0301 CTACTGAAAG GCCGAAACAG TCTGCTTTTC ACCTACGGCG TGACTGGAAG TGGAAAAACG
0361 TACACCATGA CCGGCAACCT GCGACACCGC GGCATCATGC CGCGCTGCCT GGACGTGCTC
0421 TTTCGCACCA TTTCAGATTA CCAGGCCAAG AAGTTCGTCT TCAAGCCGGA CAAGCTTAAT
0481 GGCTTCGAGA TCCTGTCCGA AG |
| Assemble Data |
<PRE>
1258R-1.IR_full 1 AACTCCGCGGGTGCATCAAACGGTGGAGAAACAGCGACGAGACACCTCCGACAAGGCCAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1258R-1.in silico 1 AACTCCGCGGGTGCATCAAACGGTGGAGAAACAGCGACGAGACACCTCCGACAAGGCCAG 60
1258R-1.IR_full 61 GGATCCAGTGAATGTGTTCTGTCGAGTGCGGCCACTACAATCCGACGCAGATCTCACCTC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1258R-1.in silico 61 GGATCCAGTGAATGTGTTCTGTCGAGTGCGGCCACTACAATCCGACGCAGATCTCACCTC 120
1258R-1.IR_full 121 CCTAAGGGTCAAGAACTCCACGACGATCGCACTGAATCCGCAGGATCAGCTGCTGCAGCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1258R-1.in silico 121 CCTAAGGGTCAAGAACTCCACGACGATCGCACTGAATCCGCAGGATCAGCTGCTGCAGCA 180
1258R-1.IR_full 181 TCACAAGCCACACAACGGCGCCCAGCGCGAGG<span class="snp"><tt>N</tt></span>TTCAGTACATATTCAAGCACGTTTTCC 240
||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||
1258R-1.in silico 181 TCACAAGCCACACAACGGCGCCCAGCGCGAGG<span class="snp"><tt>-</tt></span>TTCAGTACATATTCAAGCACGTTTTCC 240
1258R-1.IR_full 241 AGCCGGATGCCACGCAGCAGGATGTGTACGCAGCTGTGGCCCAGCCGTTGGTGG<span class="snp"><tt>N</tt></span>AGAAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|||||
1258R-1.in silico 241 AGCCGGATGCCACGCAGCAGGATGTGTACGCAGCTGTGGCCCAGCCGTTGGTGG<span class="snp"><tt>-</tt></span>AGAAC 300
1258R-1.IR_full 301 CTACTGAAAGGCCGAAACAGTCTGCTTTTCACCTACGGCGTGACTGGAAGTGGAAAAACG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1258R-1.in silico 301 CTACTGAAAGGCCGAAACAGTCTGCTTTTCACCTACGGCGTGACTGGAAGTGGAAAAACG 360
1258R-1.IR_full 361 TACACCATGACCGGCAACCTGCGACAC<span class="snp"><tt>N</tt></span>G<span class="snp"><tt>N</tt></span>GGCATCATGCCG<span class="snp"><tt>N</tt></span>GCTGCCTGGACGTGCTC 420
|||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>|<span class="snp"><tt> </tt></span>||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||
1258R-1.in silico 361 TACACCATGACCGGCAACCTGCGACAC<span class="snp"><tt>C</tt></span>G<span class="snp"><tt>C</tt></span>GGCATCATGCCG<span class="snp"><tt>C</tt></span>GCTGCCTGGACGTGCTC 420
1258R-1.IR_full 421 TTTCGCACCATTTCAGATTACCAGGCCAAGAAGTTCGTCTTCAAGCCGGACAAGCTTAAT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1258R-1.in silico 421 TTTCGCACCATTTCAGATTACCAGGCCAAGAAGTTCGTCTTCAAGCCGGACAAGCTTAAT 480
1258R-1.IR_full 481 GGCTTCGAGATCCTGTCCGAAG 502
||||||||||||||||||||||
1258R-1.in silico 481 GGCTTCGAGATCCTGTCCGAAG 502
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
4 |
6 |
NM_057677.3 |
CG1258-RA (pav), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_169197.2 |
CG31187-RA (CG31187), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_142205.2 |
CG6130-RA (CG6130), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_078851.2 |
CG4192-RA (kek3), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_176097.1 |
CG33131-RA (SCAP), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_136798.1 |
CG30021-RA (skf), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080014.2 |
CG3064-RB (futsch), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_164652.1 |
CG14011-RC, transcript variant C (CG14011), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_135099.1 |
CG14011-RA, transcript variant A (CG14011), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_164651.1 |
CG14011-RB, transcript variant B (CG14011), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_078914.2 |
CG12840-RA (Tsp42El), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_168787.1 |
CG9739-RB, transcript variant B (fz2), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_079431.2 |
CG9739-RA, transcript variant A (fz2), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_142364.1 |
CG17283-RA (CG17283), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132292.2 |
CG7246-RA (CG7246), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_080257.2 |
CG6815-RA (bor), mRNA |
| 0 |
0 |
1 |
3 |
10 |
NM_078879.2 |
CG10363-RA (TepIV), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_170412.1 |
CG2010-RA, transcript variant A (CG2010), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_143439.2 |
CG2010-RB, transcript variant B (CG2010), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_138220.1 |
CG13889-RA (CG13889), mRNA |
| 0 |
0 |
1 |
2 |
15 |
NM_130616.2 |
CG4290-RA (CG4290), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169783.1 |
CG31243-RB, transcript variant B (cpo), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169781.1 |
CG31243-RE, transcript variant E (cpo), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_080105.2 |
CG31243-RF, transcript variant F (cpo), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169782.1 |
CG31243-RA, transcript variant A (cpo), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_137854.2 |
CG4373-RA (Cyp6d2), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_167620.2 |
CG32547-RA (CG32547), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169022.1 |
CG31536-RA, transcript variant A (Cdep), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043211.1 |
CG31536-RE, transcript variant E (Cdep), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_169023.1 |
CG31536-RB, transcript variant B (Cdep), mRNA |
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme04814
:
Motor proteins
|
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