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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12562R-2 
 Symbol CG32444  Full Name CG32444 
 CG No CG32444  Old CG No CG12562 
 Synonyms CT3430, CG14576, BcDNA:GH08902, CG12562, CG32444 
 Accession No (Link to NCBI) NM_168930.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGTCGATTACCCTAGAGGAG 
 Primer Seq. 3' 
aatctagaggtaccAACCGATTCTCGTTGTCCAGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGTCGATTA CCCTAGAGGA GGAAATCTTC GGCTTGGCCG TGAATCCGTT CACCAAGTCG 
0061 CCCGAAATGG TGCGCCGGTA CACGCTTAAG AACTCCAAGG GCATGACCGT GTCAGTGATC 
0121 CAACTGGGGG CCATCATCCA GAGCGTCTGC TTGCCGGACG CCTACAAGAA GGTGGATGAT 
0181 GTGTGCCTCG GCTTCGACGA CATCGCCAGC TACTTGGCCA ACAAGGGAGC CTACATCGGT 
0241 GGGACCCTCG GACGAGTGGC CAACCGGGTg GCCAATGGCG AGTATACGTT TAACGACACG 
0301 AAAGTCTCGG TGACAAAAAA TATCCAGGAC AAATTCCAGC TGCACGGTGG CTTCATTGGA 
0361 TTCGATAGCG TCATATGGGA GGTGGTACAG AAGTCGGCCG AGGGAGTGAT ATTTCGGCAC 
0421 GTATCGCCAC ACGGACACGA GGGCTATCCG GGCAAACTGA CGTGCCTCAT CACCTACCAG 
0481 CTGGACAACG AGAATCGGTT  
 in silico PCR Fragment 
0001 ATGTCGATTA CCCTAGAGGA GGAAATCTTC GGCTTGGCCG TGAATCCGTT CACCAAGTCG 
0061 CCCGAAATGG TGCGCCGGTA CACGCTTAAG AACTCCAAGG GCATGACCGT GTCAGTGATC 
0121 CAACTGGGGG CCATCATCCA GAGCGTCTGC TTGCCGGACG CCTACAAGAA GGTGGATGAT 
0181 GTGTGCCTCG GCTTCGACGA CATCGCCAGT TACTTGGCCA ACAAGGGAGC CTACATCGGT 
0241 GGGACCCTCG GACGAGTGGC CAACCGGGTG GCCAATGGCG AGTATACGTT TAACGACACG 
0301 AAAGTCTCGG TGACAAAAAA TATCCAGGAC AAATTCCAGC TGCACGGTGG CTTCATTGGA 
0361 TTCGATAGCG TCATATGGGA GGTGGTACAG AAGTCGGCCG AGGGAGTGAT ATTTCGGCAC 
0421 GTATCGCCAC ACGGACACGA GGGCTATCCG GGCAAACTGA CGTGCCTCAT CACCTACCAG 
0481 CTGGACAACG AGAATCGGTT  
 Assemble Data 
<PRE>

12562R-2.IR_full       1   ATGTCGATTACCCTAGAGGAGGAAATCTTCGGCTTGGCCGTGAATCCGTTCACCAAGTCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12562R-2.in silico     1   ATGTCGATTACCCTAGAGGAGGAAATCTTCGGCTTGGCCGTGAATCCGTTCACCAAGTCG 60


12562R-2.IR_full       61  CCCGAAATGGTGCGCCGGTACACGCTTAAGAACTCCAAGGGCATGACCGTGTCAGTGATC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12562R-2.in silico     61  CCCGAAATGGTGCGCCGGTACACGCTTAAGAACTCCAAGGGCATGACCGTGTCAGTGATC 120


12562R-2.IR_full       121 CAACTGGGGGCCATCATCCAGAGCGTCTGCTTGCCGGACGCCTACAAGAAGGTGGATGAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12562R-2.in silico     121 CAACTGGGGGCCATCATCCAGAGCGTCTGCTTGCCGGACGCCTACAAGAAGGTGGATGAT 180


12562R-2.IR_full       181 GTGTGCCTCGGCTTCGACGACATCGCCAG<span class="snp"><tt>C</tt></span>TACTTGGCCAACAAGGGAGCCTACATCGGT 240
                           |||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||||||||
12562R-2.in silico     181 GTGTGCCTCGGCTTCGACGACATCGCCAG<span class="snp"><tt>T</tt></span>TACTTGGCCAACAAGGGAGCCTACATCGGT 240


12562R-2.IR_full       241 GGGACCCTCGGACGAGTGGCCAACCGGGTGGCCAATGGCGAGTATACGTTTAACGACACG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12562R-2.in silico     241 GGGACCCTCGGACGAGTGGCCAACCGGGTGGCCAATGGCGAGTATACGTTTAACGACACG 300


12562R-2.IR_full       301 AAAGTCTCGGTGACAAAAAATATCCAGGACAAATTCCAGCTGCACGGTGGCTTCATTGGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12562R-2.in silico     301 AAAGTCTCGGTGACAAAAAATATCCAGGACAAATTCCAGCTGCACGGTGGCTTCATTGGA 360


12562R-2.IR_full       361 TTCGATAGCGTCATATGGGAGGTGGTACAGAAGTCGGCCGAGGGAGTGATATTTCGGCAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12562R-2.in silico     361 TTCGATAGCGTCATATGGGAGGTGGTACAGAAGTCGGCCGAGGGAGTGATATTTCGGCAC 420


12562R-2.IR_full       421 GTATCGCCACACGGACACGAGGGCTATCCGGGCAAACTGACGTGCCTCATCACCTACCAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12562R-2.in silico     421 GTATCGCCACACGGACACGAGGGCTATCCGGGCAAACTGACGTGCCTCATCACCTACCAG 480


12562R-2.IR_full       481 CTGGACAACGAGAATCGGTT 500
                           ||||||||||||||||||||
12562R-2.in silico     481 CTGGACAACGAGAATCGGTT 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  4  NM_168930.1  CG32444-RA (CG32444), mRNA 
0.41   2  4  9  9  NM_139769.1  CG10467-RA (CG10467), mRNA 
0   0  16  51  96  NM_135670.1  CG4988-RA (CG4988), mRNA 
0   0  5  16  27  NM_168931.1  CG32445-RA (CG32445), mRNA 
0   0  2  4  3  NM_139622.1  CG15011-RA (CG15011), mRNA 
0   0  2  3  4  NM_135085.2  CG7382-RA (CG7382), mRNA 
0   0  2  3  2  NM_080490.3  CG6571-RA, transcript variant A (rdgC), mRNA 
0   0  2  3  2  NM_176366.1  CG6571-RB, transcript variant B (rdgC), mRNA 
0   0  2  3  2  NM_176367.1  CG6571-RC, transcript variant C (rdgC), mRNA 
0   0  2  3  2  NM_176368.1  CG6571-RD, transcript variant D (rdgC), mRNA 
0   0  2  2  5  NM_205989.2  CG4551-RB, transcript variant B (smi35A), mRNA 
0   0  2  2  5  NM_078840.4  CG4551-RA, transcript variant A (smi35A), mRNA 
0   0  2  2  5  NM_205988.2  CG4551-RC, transcript variant C (smi35A), mRNA 
0   0  2  2  5  NM_001038818.1  CG4551-RE, transcript variant E (smi35A), mRNA 
0   0  2  2  5  NM_001038817.1  CG4551-RD, transcript variant D (smi35A), mRNA 
0   0  1  3  7  NM_135691.1  CG6734-RA (CG6734), mRNA 
0   0  1  2  6  NM_134851.1  CG15394-RA (CG15394), mRNA 
0   0  1  2  6  NM_139955.2  CG7182-RA (CG7182), mRNA 
0   0  1  2  2  NM_168152.1  CG10480-RC, transcript variant C (Bj1), mRNA 
0   0  0  5  12  NM_058118.3  CG18783-RB, transcript variant B (Kr-h1), mRNA 
0   0  0  5  12  NM_058119.3  CG18783-RA, transcript variant A (Kr-h1), mRNA 
0   0  0  5  8  NM_137703.1  CG15655-RA (CG15655), mRNA 
0   0  0  5  4  NM_166032.1  CG8233-RC, transcript variant C (CG8233), mRNA 
0   0  0  5  4  NM_137083.2  CG8233-RB, transcript variant B (CG8233), mRNA 
0   0  0  5  4  NM_166031.1  CG8233-RA, transcript variant A (CG8233), mRNA 
0   0  0  5  3  NM_165535.1  CG2146-RC, transcript variant C (didum), mRNA 
0   0  0  5  3  NM_141772.1  CG6574-RA (CG6574), mRNA 
0   0  0  5  3  NM_165536.1  CG2146-RB, transcript variant B (didum), mRNA 
0   0  0  5  3  NM_057838.3  CG2146-RA, transcript variant A (didum), mRNA 
0   0  0  5  2  NM_079936.2  CG5165-RA (Pgm), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme00010 : Glycolysis / Gluconeogenesis
dme00052 : Galactose metabolism
dme01100 : Metabolic pathways

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