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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12313R-2 |
| Symbol |
CG12313 |
Full Name |
CG12313 |
| CG No |
CG12313 |
Old CG No |
CG12313 |
| Synonyms |
CG12313 |
| Accession No (Link to NCBI) |
NM_138179.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGTGGCTGGCCCAAGATTTGC |
| Primer Seq. 3' |
aatctagaggtaccGTCCGCCACATAAGCTGCTGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGTGGCTGGC CCAAGATTTG CCGCAAACTT ATTGTAACGT CGCGGTCGCT GACCTCCGGC
0061 TTGCGGCGGG CACTGGTCAA GCAGCCCCGT AAAGGCGGAG ATGTTGGAAA ACCCGGAATG
0121 GAGCTTGGGC GCNTGTTCGT GCTTTGGACT CCGTGTGAAC CTCAGCAATG CATCCGTCGT
0181 ATACGTTGGC CACCGACGCT ACTCGACGTA CGAGAAGACG TCTACGCAAA TTTTAACGAA
0241 GCTCTTTCCA CAAACCTCTG AGGAGTCCAA CGACGAGGAA AGTCGTGAGC GGCGGGAAAC
0301 TTGAGGAGGA GGAGGAGCAG AAGGAATTGG AGCGGGCATT TAGGCGGATG AAACTGGGCT
0361 TTGGGCTTTT CGGCATAGGT AGCATGTTGT TCTCGTTTTG GGCTATATAT TTCTATGGAA
0421 GGCCGTCCTT GGATGAGCAC GGAAATGAAG TAATCGACGA ATTTAGTTGT CTGCCACAGA
0481 TGCAGCAGCT TATGTGGCGG AC |
| in silico PCR Fragment |
0001 TGTGGCTGGC CCAAGATTTG CCGCAAACTT ATTGTAACGT CGCGGTCGCT GACCTCCGGC
0061 TTGCGGCGGG CACTGGTCAA GCAGCCCCGT AAAGGCGGAG ATGTTGGAAA ACCCGGAATG
0121 GAGCTTGGGC GC-TGTTCGT GCTTTGGACT CCGTGTGAAC CTCAGCAATG CATCCGTCGT
0181 ATACGTTGGC CACCGACGCT ACTCGACGTA CGAGAAGACG TCTACGCAAA TTTTAACGAA
0241 GCTCTTTCCA CAAACCTCTG AGGAGTCCAA CGACGAGGAA AGTCGTGAGC GGC-GGAAAC
0301 TTGAGGAGGA GGAGGAGCAG AAGGAATTGG AGCGGGCATT TAGGCGGATG AAACTGGGCT
0361 TTGGGCTTTT CGGCATAGGT AGCATGTTGT TCTCGTTTTG GGCTATATAT TTCTATGGAA
0421 GGCCGTCCTT GGATGAGCAC GGAAATGAAG TAATCGACGA ATTTAGTTGT CTGCCACAGA
0481 TGCAGCAGCT TATGTGGCGG AC |
| Assemble Data |
<PRE>
12313R-2.IR_full 1 TGTGGCTGGCCCAAGATTTGCCGCAAACTTATTGTAACGTCGCGGTCGCTGACCTCCGGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-2.in silico 1 TGTGGCTGGCCCAAGATTTGCCGCAAACTTATTGTAACGTCGCGGTCGCTGACCTCCGGC 60
12313R-2.IR_full 61 TTGCGGCGGGCACTGGTCAAGCAGCCCCGTAAAGGCGGAGATGTTGGAAAACCCGGAATG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-2.in silico 61 TTGCGGCGGGCACTGGTCAAGCAGCCCCGTAAAGGCGGAGATGTTGGAAAACCCGGAATG 120
12313R-2.IR_full 121 GAGCTTGGGCGC<span class="snp"><tt>N</tt></span>TGTTCGTGCTTTGGACTCCGTGTGAACCTCAGCAATGCATCCGTCGT 180
||||||||||||<span class="snp"><tt> </tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
12313R-2.in silico 121 GAGCTTGGGCGC<span class="snp"><tt>-</tt></span>TGTTCGTGCTTTGGACTCCGTGTGAACCTCAGCAATGCATCCGTCGT 180
12313R-2.IR_full 181 ATACGTTGGCCACCGACGCTACTCGACGTACGAGAAGACGTCTACGCAAATTTTAACGAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-2.in silico 181 ATACGTTGGCCACCGACGCTACTCGACGTACGAGAAGACGTCTACGCAAATTTTAACGAA 240
12313R-2.IR_full 241 GCTCTTTCCACAAACCTCTGAGGAGTCCAACGACGAGGAAAGTCGTGAGCGGC<span class="snp"><tt>G</tt></span>GGAAAC 300
|||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||
12313R-2.in silico 241 GCTCTTTCCACAAACCTCTGAGGAGTCCAACGACGAGGAAAGTCGTGAGCGGC<span class="snp"><tt>-</tt></span>GGAAAC 300
12313R-2.IR_full 301 TTGAGGAGGAGGAGGAGCAGAAGGAATTGGAGCGGGCATTTAGGCGGATGAAACTGGGCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-2.in silico 301 TTGAGGAGGAGGAGGAGCAGAAGGAATTGGAGCGGGCATTTAGGCGGATGAAACTGGGCT 360
12313R-2.IR_full 361 TTGGGCTTTTCGGCATAGGTAGCATGTTGTTCTCGTTTTGGGCTATATATTTCTATGGAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-2.in silico 361 TTGGGCTTTTCGGCATAGGTAGCATGTTGTTCTCGTTTTGGGCTATATATTTCTATGGAA 420
12313R-2.IR_full 421 GGCCGTCCTTGGATGAGCACGGAAATGAAGTAATCGACGAATTTAGTTGTCTGCCACAGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-2.in silico 421 GGCCGTCCTTGGATGAGCACGGAAATGAAGTAATCGACGAATTTAGTTGTCTGCCACAGA 480
12313R-2.IR_full 481 TGCAGCAGCTTATGTGGCGGAC 502
||||||||||||||||||||||
12313R-2.in silico 481 TGCAGCAGCTTATGTGGCGGAC 502
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
9 |
NM_138179.2 |
CG12313-RA (CG12313), mRNA |
| 0.62 |
3 |
2 |
3 |
17 |
NM_168278.1 |
CG32352-RB, transcript variant B (CG32352), mRNA |
| 0.62 |
3 |
2 |
3 |
17 |
NM_168279.2 |
CG32352-RA, transcript variant A (CG32352), mRNA |
| 0.62 |
3 |
2 |
3 |
17 |
NM_170629.2 |
CG32352-RC, transcript variant C (CG32352), mRNA |
| 0.41 |
2 |
3 |
2 |
7 |
NM_130495.2 |
CG13367-RA (CG13367), mRNA |
| 0.2 |
1 |
3 |
4 |
16 |
NM_166957.1 |
CG32793-RA (CG32793), mRNA |
| 0 |
0 |
6 |
10 |
12 |
NM_166047.1 |
CG8603-RB, transcript variant B (CG8603), mRNA |
| 0 |
0 |
6 |
10 |
12 |
NM_137108.2 |
CG8603-RA, transcript variant A (CG8603), mRNA |
| 0 |
0 |
5 |
21 |
53 |
NM_132012.1 |
CG15776-RA (CG15776), mRNA |
| 0 |
0 |
5 |
21 |
40 |
NM_132903.1 |
CG9906-RA (CG9906), mRNA |
| 0 |
0 |
5 |
18 |
20 |
NM_132529.2 |
CG2025-RA (CG2025), mRNA |
| 0 |
0 |
5 |
12 |
44 |
NM_132457.1 |
CG11122-RA (CG11122), mRNA |
| 0 |
0 |
5 |
11 |
13 |
NM_079295.2 |
CG6539-RA (Dhh1), mRNA |
| 0 |
0 |
5 |
10 |
19 |
NM_132392.1 |
CG12641-RA (CG12641), mRNA |
| 0 |
0 |
5 |
10 |
14 |
NM_132225.1 |
CG18262-RA (CG18262), mRNA |
| 0 |
0 |
4 |
24 |
30 |
NM_141658.3 |
CG8301-RA (CG8301), mRNA |
| 0 |
0 |
4 |
14 |
16 |
NM_206356.2 |
CG33261-RB, transcript variant B (Trl), mRNA |
| 0 |
0 |
4 |
14 |
15 |
NM_001038926.1 |
CG33261-RI, transcript variant I (Trl), mRNA |
| 0 |
0 |
4 |
10 |
15 |
NM_132512.2 |
CG2448-RA (FucT6), mRNA |
| 0 |
0 |
4 |
8 |
13 |
NM_132724.2 |
CG1810-RA (mRNA-capping-enzyme), mRNA |
| 0 |
0 |
4 |
5 |
15 |
NM_137763.3 |
CG17922-RA (CG17922), mRNA |
| 0 |
0 |
4 |
4 |
19 |
NM_134505.1 |
CG14230-RA (CG14230), mRNA |
| 0 |
0 |
4 |
4 |
10 |
NM_001015181.1 |
CG12459-PA (CG12459), mRNA |
| 0 |
0 |
4 |
2 |
6 |
NM_141133.1 |
CG11449-RA (CG11449), mRNA |
| 0 |
0 |
3 |
52 |
41 |
NM_167524.2 |
CG9819-RB, transcript variant B (CanA-14F), mRNA |
| 0 |
0 |
3 |
52 |
41 |
NM_167523.2 |
CG9819-RA, transcript variant A (CanA-14F), mRNA |
| 0 |
0 |
3 |
18 |
16 |
NM_134510.1 |
CG12703-RA (CG12703), mRNA |
| 0 |
0 |
3 |
12 |
21 |
NM_132962.1 |
CG8945-RC (CG8945), mRNA |
| 0 |
0 |
3 |
9 |
17 |
NM_134680.1 |
CG13691-RA (BBS8), mRNA |
| 0 |
0 |
3 |
8 |
20 |
NM_132247.2 |
CG11190-RA (CG11190), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| +- |
|
| DB |
 |
| + |
|
| DC |
 |
| + |
|
| DM |
 |
| +- |
|
| VM |
 |
| +- |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| ++ |
|
| DM |
|
| + |
|
| VM |
|
| |
|
| VB |
|
| |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
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