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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

12313R-1

Symbol

CG12313

Full Name

CG12313

CG No

CG12313

Old CG No

CG12313

Synonyms

CG12313

Accession No (Link to NCBI)

NM_138179.2

Inserted Chr.

lll

Insertional Mutation

semi-lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

semi-lethal

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.<br> An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.<br> PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGTGGCTGGCCCAAGATTTGC

Primer Seq. 3'

aatctagaggtaccGTCCGCCACATAAGCTGCTGC

Predicted Fragment Size

IR fragment full Seq

0001 TGTGGCTGGC CCAAGATTTG CCGCAAACTT ATTGTAACGT CGCGGTCGCT GACCTCCGGC 
0061 TTGCGGCGGG CACTGGTCAA GCAGCCCCGT AAAGGCGGAG ATGTTGGAAA ACCCGGAATG 
0121 GAGCTTGGGC GCNTGTTCGT GCTTTGGACT CCGTGTGAAC CTCAGCAATG CATCCGTCGT 
0181 ATACGTTGGC CACCGACGCT ACTCGACGTA CGAGAAGACG TCTACGCAAA TTTTAACGAA 
0241 GCTCTTTCCA CAAACCTCTG AGGAGTCCAA CGACGAGGAA AGTCGTGAGC GGCGGGAAAC 
0301 TTGAGGAGGA GGAGGAGCAG AAGGAATTGG AGCGGGCATT TAGGCGGATG AAACTGGGCT 
0361 TTGGGCTTTT CGGCATAGGT AGCATGTTGT TCTCGTTTTG GGCTATATAT TTCTATGGAA 
0421 GGCCGTCCTT GGATGAGCAC GGAAATGAAG TAATCGACGA ATTTAGTTGT CTGCCACAGA 
0481 TGCAGCAGCT TATGTGGCGG AC

in silico PCR Fragment

0001 TGTGGCTGGC CCAAGATTTG CCGCAAACTT ATTGTAACGT CGCGGTCGCT GACCTCCGGC 
0061 TTGCGGCGGG CACTGGTCAA GCAGCCCCGT AAAGGCGGAG ATGTTGGAAA ACCCGGAATG 
0121 GAGCTTGGGC GC-TGTTCGT GCTTTGGACT CCGTGTGAAC CTCAGCAATG CATCCGTCGT 
0181 ATACGTTGGC CACCGACGCT ACTCGACGTA CGAGAAGACG TCTACGCAAA TTTTAACGAA 
0241 GCTCTTTCCA CAAACCTCTG AGGAGTCCAA CGACGAGGAA AGTCGTGAGC GGC-GGAAAC 
0301 TTGAGGAGGA GGAGGAGCAG AAGGAATTGG AGCGGGCATT TAGGCGGATG AAACTGGGCT 
0361 TTGGGCTTTT CGGCATAGGT AGCATGTTGT TCTCGTTTTG GGCTATATAT TTCTATGGAA 
0421 GGCCGTCCTT GGATGAGCAC GGAAATGAAG TAATCGACGA ATTTAGTTGT CTGCCACAGA 
0481 TGCAGCAGCT TATGTGGCGG AC

Assemble Data

<PRE>

12313R-1.IR_full       1   TGTGGCTGGCCCAAGATTTGCCGCAAACTTATTGTAACGTCGCGGTCGCTGACCTCCGGC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-1.in silico     1   TGTGGCTGGCCCAAGATTTGCCGCAAACTTATTGTAACGTCGCGGTCGCTGACCTCCGGC 60


12313R-1.IR_full       61  TTGCGGCGGGCACTGGTCAAGCAGCCCCGTAAAGGCGGAGATGTTGGAAAACCCGGAATG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-1.in silico     61  TTGCGGCGGGCACTGGTCAAGCAGCCCCGTAAAGGCGGAGATGTTGGAAAACCCGGAATG 120


12313R-1.IR_full       121 GAGCTTGGGCGC<span class="snp"><tt>N</tt></span>TGTTCGTGCTTTGGACTCCGTGTGAACCTCAGCAATGCATCCGTCGT 180
                           ||||||||||||<span class="snp"><tt>&nbsp;</tt></span>|||||||||||||||||||||||||||||||||||||||||||||||
12313R-1.in silico     121 GAGCTTGGGCGC<span class="snp"><tt>-</tt></span>TGTTCGTGCTTTGGACTCCGTGTGAACCTCAGCAATGCATCCGTCGT 180


12313R-1.IR_full       181 ATACGTTGGCCACCGACGCTACTCGACGTACGAGAAGACGTCTACGCAAATTTTAACGAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-1.in silico     181 ATACGTTGGCCACCGACGCTACTCGACGTACGAGAAGACGTCTACGCAAATTTTAACGAA 240


12313R-1.IR_full       241 GCTCTTTCCACAAACCTCTGAGGAGTCCAACGACGAGGAAAGTCGTGAGCGGC<span class="snp"><tt>G</tt></span>GGAAAC 300
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||
12313R-1.in silico     241 GCTCTTTCCACAAACCTCTGAGGAGTCCAACGACGAGGAAAGTCGTGAGCGGC<span class="snp"><tt>-</tt></span>GGAAAC 300


12313R-1.IR_full       301 TTGAGGAGGAGGAGGAGCAGAAGGAATTGGAGCGGGCATTTAGGCGGATGAAACTGGGCT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-1.in silico     301 TTGAGGAGGAGGAGGAGCAGAAGGAATTGGAGCGGGCATTTAGGCGGATGAAACTGGGCT 360


12313R-1.IR_full       361 TTGGGCTTTTCGGCATAGGTAGCATGTTGTTCTCGTTTTGGGCTATATATTTCTATGGAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-1.in silico     361 TTGGGCTTTTCGGCATAGGTAGCATGTTGTTCTCGTTTTGGGCTATATATTTCTATGGAA 420


12313R-1.IR_full       421 GGCCGTCCTTGGATGAGCACGGAAATGAAGTAATCGACGAATTTAGTTGTCTGCCACAGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12313R-1.in silico     421 GGCCGTCCTTGGATGAGCACGGAAATGAAGTAATCGACGAATTTAGTTGTCTGCCACAGA 480


12313R-1.IR_full       481 TGCAGCAGCTTATGTGGCGGAC 502
                           ||||||||||||||||||||||
12313R-1.in silico     481 TGCAGCAGCTTATGTGGCGGAC 502

</PRE>

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	9	NM_138179.2	CG12313-RA (CG12313), mRNA
0.62	3	2	3	17	NM_168278.1	CG32352-RB, transcript variant B (CG32352), mRNA
0.62	3	2	3	17	NM_168279.2	CG32352-RA, transcript variant A (CG32352), mRNA
0.62	3	2	3	17	NM_170629.2	CG32352-RC, transcript variant C (CG32352), mRNA
0.41	2	3	2	7	NM_130495.2	CG13367-RA (CG13367), mRNA
0.2	1	3	4	16	NM_166957.1	CG32793-RA (CG32793), mRNA
0	0	6	10	12	NM_166047.1	CG8603-RB, transcript variant B (CG8603), mRNA
0	0	6	10	12	NM_137108.2	CG8603-RA, transcript variant A (CG8603), mRNA
0	0	5	21	53	NM_132012.1	CG15776-RA (CG15776), mRNA
0	0	5	21	40	NM_132903.1	CG9906-RA (CG9906), mRNA
0	0	5	18	20	NM_132529.2	CG2025-RA (CG2025), mRNA
0	0	5	12	44	NM_132457.1	CG11122-RA (CG11122), mRNA
0	0	5	11	13	NM_079295.2	CG6539-RA (Dhh1), mRNA
0	0	5	10	19	NM_132392.1	CG12641-RA (CG12641), mRNA
0	0	5	10	14	NM_132225.1	CG18262-RA (CG18262), mRNA
0	0	4	24	30	NM_141658.3	CG8301-RA (CG8301), mRNA
0	0	4	14	16	NM_206356.2	CG33261-RB, transcript variant B (Trl), mRNA
0	0	4	14	15	NM_001038926.1	CG33261-RI, transcript variant I (Trl), mRNA
0	0	4	10	15	NM_132512.2	CG2448-RA (FucT6), mRNA
0	0	4	8	13	NM_132724.2	CG1810-RA (mRNA-capping-enzyme), mRNA
0	0	4	5	15	NM_137763.3	CG17922-RA (CG17922), mRNA
0	0	4	4	19	NM_134505.1	CG14230-RA (CG14230), mRNA
0	0	4	4	10	NM_001015181.1	CG12459-PA (CG12459), mRNA
0	0	4	2	6	NM_141133.1	CG11449-RA (CG11449), mRNA
0	0	3	52	41	NM_167524.2	CG9819-RB, transcript variant B (CanA-14F), mRNA
0	0	3	52	41	NM_167523.2	CG9819-RA, transcript variant A (CanA-14F), mRNA
0	0	3	18	16	NM_134510.1	CG12703-RA (CG12703), mRNA
0	0	3	12	21	NM_132962.1	CG8945-RC (CG8945), mRNA
0	0	3	9	17	NM_134680.1	CG13691-RA (BBS8), mRNA
0	0	3	8	20	NM_132247.2	CG11190-RA (CG11190), mRNA

Pathways (updated: 2024/04/26)

KEGG Pathway

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