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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12301R-2 
 Symbol CG12301  Full Name CG12301 
 CG No CG12301  Old CG No CG12301 
 Synonyms CG12301 
 Accession No (Link to NCBI) NM_140524.2 
 Inserted Chr.
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication]
Kamimura K, Koyama T, Habuchi H, Ueda R, Masu M, Kimata K, Nakato H.<br> Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling.<br> J Cell Biol (2006) 174(6) 773-8 [ PubMed ID = 16966419 ] [ RRC reference ]

Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.<br> Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.<br> Proc Natl Acad Sci U S A (2006) 103(39) 14417-22 [ PubMed ID = 16971484 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5'
aaggcctacatggccggaccgAACCAAAGGCACACAAGAAGC 
 Primer Seq. 3'
aatctagaggtaccCCGTAGTTTACGGTTGTTAGC 
 Predicted Fragment Size
500 
 IR fragment full Seq
0001 AACCAAAGGC ACACAAGAAG CTGCTGCAGG CCATCNGcAG TCTGGGCAAG GTGCAGCACA 
0061 TCCAGAAGTC CACGCGCGAC GAgcGGCAGT CGCTCCAGGA TGAGTTCCAG CTGGTAAAAG 
0121 GTGTTTCGTC AGCGGAAACG GATCATGCCC CCAGAGCCGT GGGTCTGAAC GATCTGGTAG 
0181 ACATCCTACG CACCTCCACC AAGCACAGTC AGACGGGCAA GAAGCTCAAG AACATCCATG 
0241 GCAGCAAAAA GGTGCTACAG AAGCCGCTGG AGAAACCAGC TGCGGATCGA ATTAAACGGA 
0301 CCATTGGCTA CGAGGGAGTT ACCAAGAAAC TCGGTCGTTG GGATGCTGTG GTGGCTCAAC 
0361 AGAGATCTGC GGAGACGCAG ATCTTCCCCC TACCATCTGA AACGGTTTAC GTAAATACTG 
0421 CGGCAAATGC CAGGCCTCTA AACACCCGGG TCAAGTCCAA TCTTGCTCAA GAACTCGAGG 
0481 CTAACAACCG TAAACTACGG  
 in silico PCR Fragment
0001 AACCAAAGGC ACACAAGAAG CTGCTGCAGG CCATCGGCAG TCTGGGCAAG GTGCAGCACA 
0061 TCCAGAAGTC CACGCGCGAC GAGCGGCAGT CGCTCCAGGA TGAGTTCCAG CTGGTAAAAG 
0121 GTGTTTCGTC AGCGGAAACG GATCATGCCC CCAGAGCCGT GGGTCTGAAC GATCTGGTAG 
0181 ACATCCTACG CACCTCCACC AAGCACAGTC AGACGGGCAA GAAGCTCAAG AACATCCATG 
0241 GCAGCAAAAA GGTGCTACAG AAGCCGCTGG AGAAACCAGC TGCGGATCGA ATTAAACGGA 
0301 CCATTGGCTA CGAGGGAGTT ACCAAGAAAC TCGGTCGTTG GGATGCTGTG GTGGCTCAAC 
0361 AGAGATCTGC GGAGACGCAG ATCTTCCCCC TACCATCTGA AACGGTTTAC GTAAATACTG 
0421 CGGCAAATGC CAGGCCTCTA AACACCCGGG TCAAGTCCAA TCTTGCTCAA GAACTCGAGG 
0481 CTAACAACCG TAAACTACGG  
 Assemble Data
<PRE>

12301R-2.IR_full       1   AACCAAAGGCACACAAGAAGCTGCTGCAGGCCATC<span class="snp"><tt>N</tt></span>GCAGTCTGGGCAAGGTGCAGCACA 60
                           |||||||||||||||||||||||||||||||||||<span class="snp"><tt>&nbsp;</tt></span>||||||||||||||||||||||||
12301R-2.in silico     1   AACCAAAGGCACACAAGAAGCTGCTGCAGGCCATC<span class="snp"><tt>G</tt></span>GCAGTCTGGGCAAGGTGCAGCACA 60


12301R-2.IR_full       61  TCCAGAAGTCCACGCGCGACGAGCGGCAGTCGCTCCAGGATGAGTTCCAGCTGGTAAAAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-2.in silico     61  TCCAGAAGTCCACGCGCGACGAGCGGCAGTCGCTCCAGGATGAGTTCCAGCTGGTAAAAG 120


12301R-2.IR_full       121 GTGTTTCGTCAGCGGAAACGGATCATGCCCCCAGAGCCGTGGGTCTGAACGATCTGGTAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-2.in silico     121 GTGTTTCGTCAGCGGAAACGGATCATGCCCCCAGAGCCGTGGGTCTGAACGATCTGGTAG 180


12301R-2.IR_full       181 ACATCCTACGCACCTCCACCAAGCACAGTCAGACGGGCAAGAAGCTCAAGAACATCCATG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-2.in silico     181 ACATCCTACGCACCTCCACCAAGCACAGTCAGACGGGCAAGAAGCTCAAGAACATCCATG 240


12301R-2.IR_full       241 GCAGCAAAAAGGTGCTACAGAAGCCGCTGGAGAAACCAGCTGCGGATCGAATTAAACGGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-2.in silico     241 GCAGCAAAAAGGTGCTACAGAAGCCGCTGGAGAAACCAGCTGCGGATCGAATTAAACGGA 300


12301R-2.IR_full       301 CCATTGGCTACGAGGGAGTTACCAAGAAACTCGGTCGTTGGGATGCTGTGGTGGCTCAAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-2.in silico     301 CCATTGGCTACGAGGGAGTTACCAAGAAACTCGGTCGTTGGGATGCTGTGGTGGCTCAAC 360


12301R-2.IR_full       361 AGAGATCTGCGGAGACGCAGATCTTCCCCCTACCATCTGAAACGGTTTACGTAAATACTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-2.in silico     361 AGAGATCTGCGGAGACGCAGATCTTCCCCCTACCATCTGAAACGGTTTACGTAAATACTG 420


12301R-2.IR_full       421 CGGCAAATGCCAGGCCTCTAAACACCCGGGTCAAGTCCAATCTTGCTCAAGAACTCGAGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-2.in silico     421 CGGCAAATGCCAGGCCTCTAAACACCCGGGTCAAGTCCAATCTTGCTCAAGAACTCGAGG 480


12301R-2.IR_full       481 CTAACAACCGTAAACTACGG 500
                           ||||||||||||||||||||
12301R-2.in silico     481 CTAACAACCGTAAACTACGG 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  NM_140524.2  CG12301-RA (CG12301), mRNA 
0   NM_140489.2  CG6778-RA, transcript variant A (Aats-gly), mRNA 
0   NM_168605.1  CG6778-RB, transcript variant B (Aats-gly), mRNA 
0   NM_140326.1  CG10654-RA (CG10654), mRNA 
0   NM_206262.1  CG12734-RB, transcript variant B (CG12734), mRNA 
0   NM_139523.1  CG12734-RA, transcript variant A (CG12734), mRNA 
0   NM_144290.1  CG12643-RA (CG12643), mRNA 
0   NM_169704.1  CG32855-RA (CG32855), mRNA 
0   NM_132777.1  CG9091-RA (RpL37a), mRNA 
0   NM_079615.2  CG7756-RA (Hsc70-2), mRNA 
0   NM_139424.1  CG11814-RA (CG11814), mRNA 
0   NM_079768.2  CG6238-RA, transcript variant A (ssh), mRNA 
0   NM_170184.1  CG6238-RB, transcript variant B (ssh), mRNA 
0   NM_136344.1  CG14470-RA (CG14470), mRNA 
0   10  NM_169583.1  CG31319-RA (RhoGAP88C), mRNA 
0   NM_078659.3  CG5055-RA, transcript variant A (baz), mRNA 
0   NM_001032021.1  CG31043-RC, transcript variant C (gukh), mRNA 
0   NM_169845.1  CG31043-RA, transcript variant A (gukh), mRNA 
0   NM_169846.1  CG31043-RB, transcript variant B (gukh), mRNA 
0   NM_175968.1  CG12787-RF, transcript variant F (hoe1), mRNA 
0   NM_175967.1  CG12787-RE, transcript variant E (hoe1), mRNA 
0   NM_164603.1  CG12787-RB, transcript variant B (hoe1), mRNA 
0   NM_175966.1  CG12787-RD, transcript variant D (hoe1), mRNA 
0   NM_140176.1  CG6279-RA (CG6279), mRNA 
0   10  NM_143765.2  CG12218-RA (mei-P26), mRNA 
0   NM_001014591.1  CG8103-RB, transcript variant B (Mi-2), mRNA 
0   NM_140897.2  CG8103-RA, transcript variant A (Mi-2), mRNA 
0   NM_132347.1  CG3099-RB (CG3099), mRNA 
0   NM_165232.1  CG5674-RB, transcript variant B (CG5674), mRNA 
0   NM_136012.4  CG5674-RA, transcript variant A (CG5674), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway dme03008 : Ribosome biogenesis in eukaryotes

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