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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
12301R-1 |
| Symbol |
CG12301 |
Full Name |
CG12301 |
| CG No |
CG12301 |
Old CG No |
CG12301 |
| Synonyms |
CG12301 |
| Accession No (Link to NCBI) |
NM_140524.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
semi-lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Ibaraki K, Nakatsuka M, Ohsako T, Watanabe M, Miyazaki Y, Shirakami M, Karr TL, Sanuki R, Tomaru M, Takano-Shimizu-Kouno T.<br> A cross-species approach for the identification of Drosophila male sterility genes.<br> G3 (Bethesda) (2021) 11(8)
[
PubMed ID = 34849808
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Kamimura K, Koyama T, Habuchi H, Ueda R, Masu M, Kimata K, Nakato H.<br> Specific and flexible roles of heparan sulfate modifications in Drosophila FGF signaling.<br> J Cell Biol (2006) 174(6) 773-8
[
PubMed ID = 16966419
]
[
RRC reference
]
Kobayashi M, Michaut L, Ino A, Honjo K, Nakajima T, Maruyama Y, Mochizuki H, Ando M, Ghangrekar I, Takahashi K, Saigo K, Ueda R, Gehring WJ, Furukubo-Tokunaga K.<br> Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation.<br> Proc Natl Acad Sci U S A (2006) 103(39) 14417-22
[
PubMed ID = 16971484
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAACCAAAGGCACACAAGAAGC |
| Primer Seq. 3' |
aatctagaggtaccCCGTAGTTTACGGTTGTTAGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AACCAAAGGC ACACAAGAAG CTGCTGCAGG CCATCNGcAG TCTGGGCAAG GTGCAGCACA
0061 TCCAGAAGTC CACGCGCGAC GAgcGGCAGT CGCTCCAGGA TGAGTTCCAG CTGGTAAAAG
0121 GTGTTTCGTC AGCGGAAACG GATCATGCCC CCAGAGCCGT GGGTCTGAAC GATCTGGTAG
0181 ACATCCTACG CACCTCCACC AAGCACAGTC AGACGGGCAA GAAGCTCAAG AACATCCATG
0241 GCAGCAAAAA GGTGCTACAG AAGCCGCTGG AGAAACCAGC TGCGGATCGA ATTAAACGGA
0301 CCATTGGCTA CGAGGGAGTT ACCAAGAAAC TCGGTCGTTG GGATGCTGTG GTGGCTCAAC
0361 AGAGATCTGC GGAGACGCAG ATCTTCCCCC TACCATCTGA AACGGTTTAC GTAAATACTG
0421 CGGCAAATGC CAGGCCTCTA AACACCCGGG TCAAGTCCAA TCTTGCTCAA GAACTCGAGG
0481 CTAACAACCG TAAACTACGG |
| in silico PCR Fragment |
0001 AACCAAAGGC ACACAAGAAG CTGCTGCAGG CCATCGGCAG TCTGGGCAAG GTGCAGCACA
0061 TCCAGAAGTC CACGCGCGAC GAGCGGCAGT CGCTCCAGGA TGAGTTCCAG CTGGTAAAAG
0121 GTGTTTCGTC AGCGGAAACG GATCATGCCC CCAGAGCCGT GGGTCTGAAC GATCTGGTAG
0181 ACATCCTACG CACCTCCACC AAGCACAGTC AGACGGGCAA GAAGCTCAAG AACATCCATG
0241 GCAGCAAAAA GGTGCTACAG AAGCCGCTGG AGAAACCAGC TGCGGATCGA ATTAAACGGA
0301 CCATTGGCTA CGAGGGAGTT ACCAAGAAAC TCGGTCGTTG GGATGCTGTG GTGGCTCAAC
0361 AGAGATCTGC GGAGACGCAG ATCTTCCCCC TACCATCTGA AACGGTTTAC GTAAATACTG
0421 CGGCAAATGC CAGGCCTCTA AACACCCGGG TCAAGTCCAA TCTTGCTCAA GAACTCGAGG
0481 CTAACAACCG TAAACTACGG |
| Assemble Data |
<PRE>
12301R-1.IR_full 1 AACCAAAGGCACACAAGAAGCTGCTGCAGGCCATC<span class="snp"><tt>N</tt></span>GCAGTCTGGGCAAGGTGCAGCACA 60
|||||||||||||||||||||||||||||||||||<span class="snp"><tt> </tt></span>||||||||||||||||||||||||
12301R-1.in silico 1 AACCAAAGGCACACAAGAAGCTGCTGCAGGCCATC<span class="snp"><tt>G</tt></span>GCAGTCTGGGCAAGGTGCAGCACA 60
12301R-1.IR_full 61 TCCAGAAGTCCACGCGCGACGAGCGGCAGTCGCTCCAGGATGAGTTCCAGCTGGTAAAAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-1.in silico 61 TCCAGAAGTCCACGCGCGACGAGCGGCAGTCGCTCCAGGATGAGTTCCAGCTGGTAAAAG 120
12301R-1.IR_full 121 GTGTTTCGTCAGCGGAAACGGATCATGCCCCCAGAGCCGTGGGTCTGAACGATCTGGTAG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-1.in silico 121 GTGTTTCGTCAGCGGAAACGGATCATGCCCCCAGAGCCGTGGGTCTGAACGATCTGGTAG 180
12301R-1.IR_full 181 ACATCCTACGCACCTCCACCAAGCACAGTCAGACGGGCAAGAAGCTCAAGAACATCCATG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-1.in silico 181 ACATCCTACGCACCTCCACCAAGCACAGTCAGACGGGCAAGAAGCTCAAGAACATCCATG 240
12301R-1.IR_full 241 GCAGCAAAAAGGTGCTACAGAAGCCGCTGGAGAAACCAGCTGCGGATCGAATTAAACGGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-1.in silico 241 GCAGCAAAAAGGTGCTACAGAAGCCGCTGGAGAAACCAGCTGCGGATCGAATTAAACGGA 300
12301R-1.IR_full 301 CCATTGGCTACGAGGGAGTTACCAAGAAACTCGGTCGTTGGGATGCTGTGGTGGCTCAAC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-1.in silico 301 CCATTGGCTACGAGGGAGTTACCAAGAAACTCGGTCGTTGGGATGCTGTGGTGGCTCAAC 360
12301R-1.IR_full 361 AGAGATCTGCGGAGACGCAGATCTTCCCCCTACCATCTGAAACGGTTTACGTAAATACTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-1.in silico 361 AGAGATCTGCGGAGACGCAGATCTTCCCCCTACCATCTGAAACGGTTTACGTAAATACTG 420
12301R-1.IR_full 421 CGGCAAATGCCAGGCCTCTAAACACCCGGGTCAAGTCCAATCTTGCTCAAGAACTCGAGG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12301R-1.in silico 421 CGGCAAATGCCAGGCCTCTAAACACCCGGGTCAAGTCCAATCTTGCTCAAGAACTCGAGG 480
12301R-1.IR_full 481 CTAACAACCGTAAACTACGG 500
||||||||||||||||||||
12301R-1.in silico 481 CTAACAACCGTAAACTACGG 500
</PRE>
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_140524.2 |
CG12301-RA (CG12301), mRNA |
| 0 |
0 |
3 |
7 |
4 |
NM_140489.2 |
CG6778-RA, transcript variant A (Aats-gly), mRNA |
| 0 |
0 |
3 |
7 |
4 |
NM_168605.1 |
CG6778-RB, transcript variant B (Aats-gly), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_140326.1 |
CG10654-RA (CG10654), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_206262.1 |
CG12734-RB, transcript variant B (CG12734), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_139523.1 |
CG12734-RA, transcript variant A (CG12734), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_144290.1 |
CG12643-RA (CG12643), mRNA |
| 0 |
0 |
1 |
6 |
6 |
NM_169704.1 |
CG32855-RA (CG32855), mRNA |
| 0 |
0 |
1 |
6 |
3 |
NM_132777.1 |
CG9091-RA (RpL37a), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_079615.2 |
CG7756-RA (Hsc70-2), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_139424.1 |
CG11814-RA (CG11814), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079768.2 |
CG6238-RA, transcript variant A (ssh), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_170184.1 |
CG6238-RB, transcript variant B (ssh), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136344.1 |
CG14470-RA (CG14470), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_169583.1 |
CG31319-RA (RhoGAP88C), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_078659.3 |
CG5055-RA, transcript variant A (baz), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001032021.1 |
CG31043-RC, transcript variant C (gukh), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169845.1 |
CG31043-RA, transcript variant A (gukh), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169846.1 |
CG31043-RB, transcript variant B (gukh), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_175968.1 |
CG12787-RF, transcript variant F (hoe1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_175967.1 |
CG12787-RE, transcript variant E (hoe1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164603.1 |
CG12787-RB, transcript variant B (hoe1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_175966.1 |
CG12787-RD, transcript variant D (hoe1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140176.1 |
CG6279-RA (CG6279), mRNA |
| 0 |
0 |
0 |
5 |
10 |
NM_143765.2 |
CG12218-RA (mei-P26), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_001014591.1 |
CG8103-RB, transcript variant B (Mi-2), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_140897.2 |
CG8103-RA, transcript variant A (Mi-2), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_132347.1 |
CG3099-RB (CG3099), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_165232.1 |
CG5674-RB, transcript variant B (CG5674), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_136012.4 |
CG5674-RA, transcript variant A (CG5674), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| ++ |
|
| VM |
 |
| +- |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| |
|
| DB |
|
| ++ |
|
| DC |
|
| ++ |
|
| DM |
|
| ++ |
|
| VM |
|
| + |
|
| VB |
|
| ++ |
|
|
| Dcompartment |
|
Penetrance |
| ++ |
|
|
|
|
|
Pathways (updated: 2024/04/26)
|
| KEGG Pathway |
dme03008
:
Ribosome biogenesis in eukaryotes
|
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|
