National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock discarded request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1221R-6 
 Symbol miple  Full Name miple 
 CG No CG1221  Old CG No CG1221 
 Synonyms anon-EST:Posey203, CG1221, miple, miple1 
 Accession No (Link to NCBI) NM_138178.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCTGGCATCGCTAGTGACTTG 
 Primer Seq. 3' 
aatctagaggtaccTGTTCTTCCGCTGCTCCTTGG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCTGGCATCG CTAGTGACTT GGTCGGGGGT GATGTGCAGC ACGGTACTGG GCACCACAGA 
0061 AGGCCAGGAA ACACCTTTGG CCCTTCCTGT GGCGGAGCAG ACGCAGCCCA CTACAGCCAT 
0121 ACAAGGAGAA GTATGGGAGG AGGACGATCA CGAAGTGCTT ATAAGAAATG AACGTGGCAC 
0181 CAAGAGCGAC GGTCTGTCTT GTCGATATGG CAAAAATCCG TGGACTGAGT GTGACACCAA 
0241 AACCAATACG CGTTCAAGAA CATTGACATT AAAGAAGGGC GACCCGGCAT GCGACCAAAC 
0301 GCGAACCATT CAGAAGAAAT GTAAAAAAGC CTGTCGATAT GAAAAGGGAT CTTGGTCGGA 
0361 ATGTGCAACC GGACAAATGA CTAGAGCTGA TAAGTTGAAA GCGAGCAGTG ATCCGTCCTG 
0421 CGAAGCCACG CGGGTCATCA AGAAGAACTG CAAGCCCGGA AAGTCCAAGG ACAAAAGTGC 
0481 CAAGGAGCAG CGGAAGAACA  
 in silico PCR Fragment 
0001 CCTGGCATCG CTAGTGACTT GGTCGGGGGT GATGTGCAGC ACGGTACTGG GCACCACAGA 
0061 AGGCCAGGAA ACACCTTTGG CCCTTCCTGT GGCGGAGCAG ACGCAGCCCA CTACAGCCAT 
0121 ACAAGGAGAA GTATGGGAGG AGGACGATCA CGAAGTGCTT ATAAGAAATG AACGTGGCAC 
0181 CAAGAGCGAC GGTCTGTCTT GTCGATATGG CAAAAATCCG TGGACTGAGT GTGACACCAA 
0241 AACCAATACG CGTTCAAGAA CATTGACATT AAAGAAGGGC GACCCGGCAT GCGACCAAAC 
0301 GCGAACCATT CAGAAGAAAT GTAAAAAAGC CTGTCGATAT GAAAAGGGAT CTTGGTCGGA 
0361 ATGTGCAACC GGACAAATGA CTAGAGCTGA TAAGTTGAAA GCGAGCAGTG ATCCGTCCTG 
0421 CGAAGCCACG CGGGTCATCA AGAAGAACTG CAAGCCCGGA AAGTCCAAGG ACAAAAGTGC 
0481 CAAGGAGCAG CGGAAGAACA  
 Assemble Data 
<PRE>

1221R-6.IR_full       1   CCTGGCATCGCTAGTGACTTGGTCGGGGGTGATGTGCAGCACGGTACTGGGCACCACAGA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-6.in silico     1   CCTGGCATCGCTAGTGACTTGGTCGGGGGTGATGTGCAGCACGGTACTGGGCACCACAGA 60


1221R-6.IR_full       61  AGGCCAGGAAACACCTTTGGCCCTTCCTGTGGCGGAGCAGACGCAGCCCACTACAGCCAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-6.in silico     61  AGGCCAGGAAACACCTTTGGCCCTTCCTGTGGCGGAGCAGACGCAGCCCACTACAGCCAT 120


1221R-6.IR_full       121 ACAAGGAGAAGTATGGGAGGAGGACGATCACGAAGTGCTTATAAGAAATGAACGTGGCAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-6.in silico     121 ACAAGGAGAAGTATGGGAGGAGGACGATCACGAAGTGCTTATAAGAAATGAACGTGGCAC 180


1221R-6.IR_full       181 CAAGAGCGACGGTCTGTCTTGTCGATATGGCAAAAATCCGTGGACTGAGTGTGACACCAA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-6.in silico     181 CAAGAGCGACGGTCTGTCTTGTCGATATGGCAAAAATCCGTGGACTGAGTGTGACACCAA 240


1221R-6.IR_full       241 AACCAATACGCGTTCAAGAACATTGACATTAAAGAAGGGCGACCCGGCATGCGACCAAAC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-6.in silico     241 AACCAATACGCGTTCAAGAACATTGACATTAAAGAAGGGCGACCCGGCATGCGACCAAAC 300


1221R-6.IR_full       301 GCGAACCATTCAGAAGAAATGTAAAAAAGCCTGTCGATATGAAAAGGGATCTTGGTCGGA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-6.in silico     301 GCGAACCATTCAGAAGAAATGTAAAAAAGCCTGTCGATATGAAAAGGGATCTTGGTCGGA 360


1221R-6.IR_full       361 ATGTGCAACCGGACAAATGACTAGAGCTGATAAGTTGAAAGCGAGCAGTGATCCGTCCTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-6.in silico     361 ATGTGCAACCGGACAAATGACTAGAGCTGATAAGTTGAAAGCGAGCAGTGATCCGTCCTG 420


1221R-6.IR_full       421 CGAAGCCACGCGGGTCATCAAGAAGAACTGCAAGCCCGGAAAGTCCAAGGACAAAAGTGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-6.in silico     421 CGAAGCCACGCGGGTCATCAAGAAGAACTGCAAGCCCGGAAAGTCCAAGGACAAAAGTGC 480


1221R-6.IR_full       481 CAAGGAGCAGCGGAAGAACA 500
                          ||||||||||||||||||||
1221R-6.in silico     481 CAAGGAGCAGCGGAAGAACA 500

</PRE>
 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_138178.2  CG1221-RA, transcript variant A (miple), mRNA 
100   482  0  0  0  NM_206225.1  CG1221-RB, transcript variant B (miple), mRNA 
0   0  2  5  2  NM_132114.1  CG14442-RA (CG14442), mRNA 
0   0  1  3  2  NM_134942.2  CG17593-RA (CG17593), mRNA 
0   0  1  2  4  NM_001014545.1  CG33519-RB (Unc-89), mRNA 
0   0  1  2  2  NM_139510.2  CG11486-RG, transcript variant G (CG11486), mRNA 
0   0  1  2  2  NM_165304.1  CG10084-RB, transcript variant B (swm), mRNA 
0   0  1  2  2  NM_136132.4  CG10084-RA, transcript variant A (swm), mRNA 
0   0  1  2  2  NM_167975.1  CG11486-RJ, transcript variant J (CG11486), mRNA 
0   0  1  2  2  NM_167978.1  CG11486-RN, transcript variant N (CG11486), mRNA 
0   0  1  2  2  NM_167979.1  CG11486-RL, transcript variant L (CG11486), mRNA 
0   0  1  2  2  NM_167976.1  CG11486-RK, transcript variant K (CG11486), mRNA 
0   0  1  2  2  NM_206260.1  CG11486-RH, transcript variant H (CG11486), mRNA 
0   0  1  2  2  NM_206261.1  CG11486-RF, transcript variant F (CG11486), mRNA 
0   0  1  2  2  NM_167977.1  CG11486-RM, transcript variant M (CG11486), mRNA 
0   0  0  9  29  NM_167319.3  CG32656-RA (CG32656), mRNA 
0   0  0  4  4  NM_080051.2  CG1214-RA (ru), mRNA 
0   0  0  4  3  NM_137128.2  CG12864-RA, transcript variant A (Su(var)2-HP2), mRNA 
0   0  0  4  3  NM_136774.1  CG12325-RA (CG12325), mRNA 
0   0  0  4  3  NM_080160.2  CG11719-RA (Mst98Ca), mRNA 
0   0  0  4  3  NM_079815.2  CG18396-RA (Mst98Cb), mRNA 
0   0  0  4  2  NM_001015241.1  CG17170-PA.3 (CG17170), mRNA 
0   0  0  4  2  NM_001015240.1  CG17170-PB.3 (CG17170), mRNA 
0   0  0  4  2  NM_001015242.1  CG17170-PC.3 (CG17170), mRNA 
0   0  0  4  1  NM_140465.2  CG9311-RA (CG9311), mRNA 
0   0  0  3  11  NM_132412.1  CG9817-RA (CG9817), mRNA 
0   0  0  3  9  NM_136375.1  CG3273-RA (sced), mRNA 
0   0  0  3  8  NM_137621.2  CG10444-RA (CG10444), mRNA 
0   0  0  3  7  NM_140888.2  CG8743-RA (CG8743), mRNA 
0   0  0  3  7  NM_079205.3  CG7507-RA, transcript variant A (Dhc64C), mRNA 
 Pathways (updated: 2024/04/26)
 KEGG Pathway

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.