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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1221R-1 |
| Symbol |
miple |
Full Name |
miple |
| CG No |
CG1221 |
Old CG No |
CG1221 |
| Synonyms |
anon-EST:Posey203, CG1221, miple, miple1 |
| Accession No (Link to NCBI) |
NM_138178.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.<br> A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.<br> PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.<br> RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.<br> Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCTGGCATCGCTAGTGACTTG |
| Primer Seq. 3' |
aatctagaggtaccTGTTCTTCCGCTGCTCCTTGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCTGGCATCG CTAGTGACTT GGTCGGGGGT GATGTGCAGC ACGGTACTGG GCACCACAGA
0061 AGGCCAGGAA ACACCTTTGG CCCTTCCTGT GGCGGAGCAG ACGCAGCCCA CTACAGCCAT
0121 ACAAGGAGAA GTATGGGAGG AGGACGATCA CGAAGTGCTT ATAAGAAATG AACGTGGCAC
0181 CAAGAGCGAC GGTCTGTCTT GTCGATATGG CAAAAATCCG TGGACTGAGT GTGACACCAA
0241 AACCAATACG CGTTCAAGAA CATTGACATT AAAGAAGGGC GACCCGGCAT GCGACCAAAC
0301 GCGAACCATT CAGAAGAAAT GTAAAAAAGC CTGTCGATAT GAAAAGGGAT CTTGGTCGGA
0361 ATGTGCAACC GGACAAATGA CTAGAGCTGA TAAGTTGAAA GCGAGCAGTG ATCCGTCCTG
0421 CGAAGCCACG CGGGTCATCA AGAAGAACTG CAAGCCCGGA AAGTCCAAGG ACAAAAGTGC
0481 CAAGGAGCAG CGGAAGAACA |
| in silico PCR Fragment |
0001 CCTGGCATCG CTAGTGACTT GGTCGGGGGT GATGTGCAGC ACGGTACTGG GCACCACAGA
0061 AGGCCAGGAA ACACCTTTGG CCCTTCCTGT GGCGGAGCAG ACGCAGCCCA CTACAGCCAT
0121 ACAAGGAGAA GTATGGGAGG AGGACGATCA CGAAGTGCTT ATAAGAAATG AACGTGGCAC
0181 CAAGAGCGAC GGTCTGTCTT GTCGATATGG CAAAAATCCG TGGACTGAGT GTGACACCAA
0241 AACCAATACG CGTTCAAGAA CATTGACATT AAAGAAGGGC GACCCGGCAT GCGACCAAAC
0301 GCGAACCATT CAGAAGAAAT GTAAAAAAGC CTGTCGATAT GAAAAGGGAT CTTGGTCGGA
0361 ATGTGCAACC GGACAAATGA CTAGAGCTGA TAAGTTGAAA GCGAGCAGTG ATCCGTCCTG
0421 CGAAGCCACG CGGGTCATCA AGAAGAACTG CAAGCCCGGA AAGTCCAAGG ACAAAAGTGC
0481 CAAGGAGCAG CGGAAGAACA |
| Assemble Data |
<PRE>
1221R-1.IR_full 1 CCTGGCATCGCTAGTGACTTGGTCGGGGGTGATGTGCAGCACGGTACTGGGCACCACAGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-1.in silico 1 CCTGGCATCGCTAGTGACTTGGTCGGGGGTGATGTGCAGCACGGTACTGGGCACCACAGA 60
1221R-1.IR_full 61 AGGCCAGGAAACACCTTTGGCCCTTCCTGTGGCGGAGCAGACGCAGCCCACTACAGCCAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-1.in silico 61 AGGCCAGGAAACACCTTTGGCCCTTCCTGTGGCGGAGCAGACGCAGCCCACTACAGCCAT 120
1221R-1.IR_full 121 ACAAGGAGAAGTATGGGAGGAGGACGATCACGAAGTGCTTATAAGAAATGAACGTGGCAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-1.in silico 121 ACAAGGAGAAGTATGGGAGGAGGACGATCACGAAGTGCTTATAAGAAATGAACGTGGCAC 180
1221R-1.IR_full 181 CAAGAGCGACGGTCTGTCTTGTCGATATGGCAAAAATCCGTGGACTGAGTGTGACACCAA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-1.in silico 181 CAAGAGCGACGGTCTGTCTTGTCGATATGGCAAAAATCCGTGGACTGAGTGTGACACCAA 240
1221R-1.IR_full 241 AACCAATACGCGTTCAAGAACATTGACATTAAAGAAGGGCGACCCGGCATGCGACCAAAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-1.in silico 241 AACCAATACGCGTTCAAGAACATTGACATTAAAGAAGGGCGACCCGGCATGCGACCAAAC 300
1221R-1.IR_full 301 GCGAACCATTCAGAAGAAATGTAAAAAAGCCTGTCGATATGAAAAGGGATCTTGGTCGGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-1.in silico 301 GCGAACCATTCAGAAGAAATGTAAAAAAGCCTGTCGATATGAAAAGGGATCTTGGTCGGA 360
1221R-1.IR_full 361 ATGTGCAACCGGACAAATGACTAGAGCTGATAAGTTGAAAGCGAGCAGTGATCCGTCCTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-1.in silico 361 ATGTGCAACCGGACAAATGACTAGAGCTGATAAGTTGAAAGCGAGCAGTGATCCGTCCTG 420
1221R-1.IR_full 421 CGAAGCCACGCGGGTCATCAAGAAGAACTGCAAGCCCGGAAAGTCCAAGGACAAAAGTGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1221R-1.in silico 421 CGAAGCCACGCGGGTCATCAAGAAGAACTGCAAGCCCGGAAAGTCCAAGGACAAAAGTGC 480
1221R-1.IR_full 481 CAAGGAGCAGCGGAAGAACA 500
||||||||||||||||||||
1221R-1.in silico 481 CAAGGAGCAGCGGAAGAACA 500
</PRE>
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_138178.2 |
CG1221-RA, transcript variant A (miple), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_206225.1 |
CG1221-RB, transcript variant B (miple), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_132114.1 |
CG14442-RA (CG14442), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_134942.2 |
CG17593-RA (CG17593), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001014545.1 |
CG33519-RB (Unc-89), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_139510.2 |
CG11486-RG, transcript variant G (CG11486), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165304.1 |
CG10084-RB, transcript variant B (swm), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_136132.4 |
CG10084-RA, transcript variant A (swm), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167975.1 |
CG11486-RJ, transcript variant J (CG11486), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167978.1 |
CG11486-RN, transcript variant N (CG11486), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167979.1 |
CG11486-RL, transcript variant L (CG11486), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167976.1 |
CG11486-RK, transcript variant K (CG11486), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206260.1 |
CG11486-RH, transcript variant H (CG11486), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206261.1 |
CG11486-RF, transcript variant F (CG11486), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167977.1 |
CG11486-RM, transcript variant M (CG11486), mRNA |
| 0 |
0 |
0 |
9 |
29 |
NM_167319.3 |
CG32656-RA (CG32656), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_080051.2 |
CG1214-RA (ru), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_137128.2 |
CG12864-RA, transcript variant A (Su(var)2-HP2), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_136774.1 |
CG12325-RA (CG12325), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_080160.2 |
CG11719-RA (Mst98Ca), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_079815.2 |
CG18396-RA (Mst98Cb), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001015241.1 |
CG17170-PA.3 (CG17170), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001015240.1 |
CG17170-PB.3 (CG17170), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001015242.1 |
CG17170-PC.3 (CG17170), mRNA |
| 0 |
0 |
0 |
4 |
1 |
NM_140465.2 |
CG9311-RA (CG9311), mRNA |
| 0 |
0 |
0 |
3 |
11 |
NM_132412.1 |
CG9817-RA (CG9817), mRNA |
| 0 |
0 |
0 |
3 |
9 |
NM_136375.1 |
CG3273-RA (sced), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_137621.2 |
CG10444-RA (CG10444), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_140888.2 |
CG8743-RA (CG8743), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_079205.3 |
CG7507-RA, transcript variant A (Dhc64C), mRNA |
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| Wing Disc |
GFP
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Puc
 |
Caspase
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DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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Pathways (updated: 2024/04/26)
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| KEGG Pathway |
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